为了创制辣椒耐冷材料与选育耐冷品种,本研究以分属于5个栽培种的14份辣椒自交系为试验材料,进行4℃冷胁迫处理,测定了超氧化物歧化酶(SOD)活性、过氧化物酶(POD)活性、丙二醛(MDA)含量、脯氨酸(Pro)含量、过氧化氢(H 2 O 2)含量、氧自...为了创制辣椒耐冷材料与选育耐冷品种,本研究以分属于5个栽培种的14份辣椒自交系为试验材料,进行4℃冷胁迫处理,测定了超氧化物歧化酶(SOD)活性、过氧化物酶(POD)活性、丙二醛(MDA)含量、脯氨酸(Pro)含量、过氧化氢(H 2 O 2)含量、氧自由基(OFR)产生速率,并对辣椒的耐冷性进行了综合评价。结果表明:在4℃±1℃冷胁迫条件下,POD与SOD、MDA、H 2 O 2间,以及Pro与MDA、H 2 O 2间均存在显著相关性;在4℃胁迫12 h时,SOD、MDA、Pro的变异系数均达到最大值,因此12 h为辣椒冷胁迫处理的适宜时间。利用隶属函数法和聚类分析法,在欧氏距离0.09处将14份辣椒材料分为耐冷(Ⅰ)、中等(Ⅱ)、冷敏(Ⅲ)3个类别,其中5份材料隶属于类别Ⅰ,分别为C.annuum的‘P70’、‘A20’和‘A136’,C.chinense的‘PI241650’,以及C.frutescens‘PI439512’。展开更多
The growths of algae under different concentrations and treatments of wheat bran were studied in order to investigate the feasibility of wheat bran as the sole nutrient source of Platymonas helgolandica var.tsingtaoen...The growths of algae under different concentrations and treatments of wheat bran were studied in order to investigate the feasibility of wheat bran as the sole nutrient source of Platymonas helgolandica var.tsingtaoensis.The results showed that adding wheat bran directly to the algal fluid can promote algal growth.The algal growth was inhibited for the first 10 d and accelerated dramatically thereafter.For the comparison between the algae whose growth was modified by using wheat bran packed in a teabag or boulting cloth bag,and that by using loose wheat bran only,results showed that,at the same concentration,the algae grew faster and without lag phase when the wheat bran was packed in a teabag or a boulting cloth bag than when loose wheat bran was used only.In addition,when the wheat bran was packed in a boulting bag,the algal growth was slightly better than when the wheat bran was packed in a teabag.When the concentration of wheat bran ranged from 0.5 to 4 g/L,the cell density rose with the increase of the concentration of the wheat bran,however,the algal growth was inhibited completely at 8 g/L.At a concentration of 4 g/L,the fast-growth phase of the algae with the wheat bran aerobic pretreatment started 6 d earlier than that with wheat bran packed in a teabag,whereas there was no significant difference between them in final cell density.In terms of cell growth,the algal growth at 1 g/L wheat bran was close to that at f/2 medium during the experimental period.The study showed that it is feasible to use wheat bran as the sole nutrient source for the culture of Platymonas helgolandica var.tsingtaoensis.展开更多
文摘为了创制辣椒耐冷材料与选育耐冷品种,本研究以分属于5个栽培种的14份辣椒自交系为试验材料,进行4℃冷胁迫处理,测定了超氧化物歧化酶(SOD)活性、过氧化物酶(POD)活性、丙二醛(MDA)含量、脯氨酸(Pro)含量、过氧化氢(H 2 O 2)含量、氧自由基(OFR)产生速率,并对辣椒的耐冷性进行了综合评价。结果表明:在4℃±1℃冷胁迫条件下,POD与SOD、MDA、H 2 O 2间,以及Pro与MDA、H 2 O 2间均存在显著相关性;在4℃胁迫12 h时,SOD、MDA、Pro的变异系数均达到最大值,因此12 h为辣椒冷胁迫处理的适宜时间。利用隶属函数法和聚类分析法,在欧氏距离0.09处将14份辣椒材料分为耐冷(Ⅰ)、中等(Ⅱ)、冷敏(Ⅲ)3个类别,其中5份材料隶属于类别Ⅰ,分别为C.annuum的‘P70’、‘A20’和‘A136’,C.chinense的‘PI241650’,以及C.frutescens‘PI439512’。
文摘The growths of algae under different concentrations and treatments of wheat bran were studied in order to investigate the feasibility of wheat bran as the sole nutrient source of Platymonas helgolandica var.tsingtaoensis.The results showed that adding wheat bran directly to the algal fluid can promote algal growth.The algal growth was inhibited for the first 10 d and accelerated dramatically thereafter.For the comparison between the algae whose growth was modified by using wheat bran packed in a teabag or boulting cloth bag,and that by using loose wheat bran only,results showed that,at the same concentration,the algae grew faster and without lag phase when the wheat bran was packed in a teabag or a boulting cloth bag than when loose wheat bran was used only.In addition,when the wheat bran was packed in a boulting bag,the algal growth was slightly better than when the wheat bran was packed in a teabag.When the concentration of wheat bran ranged from 0.5 to 4 g/L,the cell density rose with the increase of the concentration of the wheat bran,however,the algal growth was inhibited completely at 8 g/L.At a concentration of 4 g/L,the fast-growth phase of the algae with the wheat bran aerobic pretreatment started 6 d earlier than that with wheat bran packed in a teabag,whereas there was no significant difference between them in final cell density.In terms of cell growth,the algal growth at 1 g/L wheat bran was close to that at f/2 medium during the experimental period.The study showed that it is feasible to use wheat bran as the sole nutrient source for the culture of Platymonas helgolandica var.tsingtaoensis.
