Applying iodine fertilizers to cultivate iodine-rich crops for daily intake is an effective approach for iodine supplementation,especially for aromatic rice.Field experiments were conducted during the early growing se...Applying iodine fertilizers to cultivate iodine-rich crops for daily intake is an effective approach for iodine supplementation,especially for aromatic rice.Field experiments were conducted during the early growing seasons of 2021 and 2022 to evaluate the impacts of foliar application of iodine fertilizer on aromatic rice and to explore the optimal iodine fertilizer concentration.At the full heading stage,six different concentrations of sodium iodide solutions of 0%(CK),0.010%(T1),0.025%(T2),0.050%(T3),0.075%(T4),and 0.100%(T5)were applied to indica aromatic rice cultivars Meixiangzhan 2 and Xiangyaxiangzhan.The results showed that sodium iodide treatments significantly increased the iodine and sodium contents in both leaves and grains.Compared with the CK,the T1 and T2 treatments increased the 2-acetyl-1-pyrroline(2-AP)content in mature grains by 8.41%-101.66%and 13.58%-74.60%,respectively.Improvements in the contents of 1-pyrroline-5-carboxylic acid,proline,1-pyrroline,and methylglyoxal,as well as the activity of proline dehydrogenase were also detected.Additionally,sodium iodide treatments remarkably decreased the chalky grain rate,chalkiness area,and chalkiness degree of aromatic rice,with the T2 treatment exhibiting a 17.79%-47.42%decrease in chalkiness degree compared with the CK.Meanwhile,T1 and T2 treatments showed beneficial impacts on chlorophyll content,photosynthetic characteristics,and yield components,while T3,T4,and T5 treatments exhibited adverse effects on leaf and grain yields.The linear discriminant analysis revealed significant differences between treatments.The correlation analysis and piecewise structural equation modeling showed that the iodine and sodium influenced the photosynthetic characteristics and chlorophyll content of the leaves,thereby regulating the 2-AP biosynthesis and yield components,ultimately affecting the 2-AP content and yield.Overall,this study suggests that foliar application of 0.025%sodium iodide is an effective method to enrich the iodine content in rice grains,improve the grain aroma and appearance quality of aromatic rice,without detrimental effects on grain yield.展开更多
基金supported by the Natural Science Foundation of Hunan Province(2021JJ30399)the Key Project of Health Commission of Hunan Province(202104122479),China。
文摘目的:黑色素瘤具有高度恶性和异质性。开发特定的黑色素瘤预后预测模型对提高患者的生存率和选择治疗策略至关重要。最近,铁死亡已被证明是一种由过度脂质过氧化诱导的铁依赖性程序性细胞死亡形式。然而,铁死亡相关基因(ferroptosis-related genes,FRGs)与黑色素瘤预后的相关性仍不清晰。本研究评估FRGs在黑色素瘤中的作用,开发一种新的预后模型,旨在为黑色素瘤的个性化治疗及疗效改善提供新思路。方法:首先通过系统地表征癌症基因组图谱(The Cancer Genome Atlas,TCGA)-皮肤黑色素瘤(skin cutaneous melanoma,SKCM)中73个FRGs的遗传改变和mRNA表达。同时通过反转录聚合酶链反应和蛋白质印迹法验证筛选的特定靶基因。随后使用TCGASKCM队列构建多基因特征模型。根据特征模型将黑色素瘤患者分为高风险和低风险组,对铁死亡相关的特征模型与免疫特征、免疫治疗的疗效或药物反应进行相关分析。结果:通过分析TCGA-SKCM数据集中的黑色素瘤样本,发现FRGs在基因变异和拷贝数变异方面表现出高频率,这些变化显著影响了基因的表达。此外,与正常皮肤组织相比,在黑色素瘤组织中发现了30个显著差异表达的基因。随后使用LASSO Cox回归方法构建的FRGs相关预后模型成功识别了13个与患者总体生存预后相关的FRGs,并通过外部数据集验证了该模型的有效性。最后,功能富集和免疫响应结果分析进一步表明高风险和低风险组之间存在免疫细胞浸润、突变负担和低氧状态的显著差异,且该模型能有效预测免疫治疗响应和药物敏感性。结论:本研究建立了一种强预后预测模型,可为黑色素瘤患者的靶向治疗和免疫治疗提供新的方向。
基金supported by the National Natural Science Foundation of China(Grant No.31971843)the Technology System of Modern Agricultural Industry in Guangdong Province,China(Grant No.2020KJ105)+1 种基金the Guangzhou Science and Technology Project,Guangdong Province,China(Grant No.202103000075)the Special Rural Revitalization Funds of Guangdong Province,China(Grant No.2021KJ382)。
文摘Applying iodine fertilizers to cultivate iodine-rich crops for daily intake is an effective approach for iodine supplementation,especially for aromatic rice.Field experiments were conducted during the early growing seasons of 2021 and 2022 to evaluate the impacts of foliar application of iodine fertilizer on aromatic rice and to explore the optimal iodine fertilizer concentration.At the full heading stage,six different concentrations of sodium iodide solutions of 0%(CK),0.010%(T1),0.025%(T2),0.050%(T3),0.075%(T4),and 0.100%(T5)were applied to indica aromatic rice cultivars Meixiangzhan 2 and Xiangyaxiangzhan.The results showed that sodium iodide treatments significantly increased the iodine and sodium contents in both leaves and grains.Compared with the CK,the T1 and T2 treatments increased the 2-acetyl-1-pyrroline(2-AP)content in mature grains by 8.41%-101.66%and 13.58%-74.60%,respectively.Improvements in the contents of 1-pyrroline-5-carboxylic acid,proline,1-pyrroline,and methylglyoxal,as well as the activity of proline dehydrogenase were also detected.Additionally,sodium iodide treatments remarkably decreased the chalky grain rate,chalkiness area,and chalkiness degree of aromatic rice,with the T2 treatment exhibiting a 17.79%-47.42%decrease in chalkiness degree compared with the CK.Meanwhile,T1 and T2 treatments showed beneficial impacts on chlorophyll content,photosynthetic characteristics,and yield components,while T3,T4,and T5 treatments exhibited adverse effects on leaf and grain yields.The linear discriminant analysis revealed significant differences between treatments.The correlation analysis and piecewise structural equation modeling showed that the iodine and sodium influenced the photosynthetic characteristics and chlorophyll content of the leaves,thereby regulating the 2-AP biosynthesis and yield components,ultimately affecting the 2-AP content and yield.Overall,this study suggests that foliar application of 0.025%sodium iodide is an effective method to enrich the iodine content in rice grains,improve the grain aroma and appearance quality of aromatic rice,without detrimental effects on grain yield.
