目的:将脐带华通胶间充质干细胞(WJMSCs)移植到子宫切口瘢痕缺陷(PCSD)模型大鼠体内,探讨其治疗效果。方法:取雌性SD大鼠50只、将其随机分为3组:正常手术组(N-O组)10只、模型对照组(M-C组)20只和模型治疗组(M-T组)20只。孕第19天N-O组...目的:将脐带华通胶间充质干细胞(WJMSCs)移植到子宫切口瘢痕缺陷(PCSD)模型大鼠体内,探讨其治疗效果。方法:取雌性SD大鼠50只、将其随机分为3组:正常手术组(N-O组)10只、模型对照组(M-C组)20只和模型治疗组(M-T组)20只。孕第19天N-O组剖宫取胎后连续缝合子宫切口;M-C组与M-T组剖宫产术后子宫肌壁注射脂多糖(LPS)联合电凝(功率为10 W)子宫切缘,间断缝合切口。M-T组分A、B亚组(各10只),A组静脉注射0.5 m L+阴道灌注0.5 m L(1×10~9/L)WJMSCs,B组阴道灌注1 m L同浓度WJMSCs,每周1次,共3次;M-C亦分A、B亚组(各10只),与M-T组同样方法处理,仅将生理盐水替代WJMSCs。各组治疗后第1和4周,分别处死6和4只,留双侧子宫进行HE染色、Masson染色、肌动蛋白(actin)和细胞角蛋白免疫组化染色,通过体视学分析,检测子宫肌层和内膜的修复情况。结果:WJMSCs治疗后第1和4周,M-C组子宫肌层厚度和actin体积密度(Vv)均显著低于N-O组,肌层胶原纤维Vv和内膜缺陷率高于N-O组(P<0.05);WJMSCs治疗后第1和4周,M-T的A和B组的肌层厚度和actin Vv均显著高于M-C组,肌层胶原纤维Vv低于M-C组;M-C组内膜缺陷率高于其它组(P<0.05)。结论:WJMSCs可促进PCSD大鼠子宫肌层结构和功能的修复。静脉+局部联合治疗和单纯阴道治疗效果无明显差别。展开更多
Objective:To excavate the medical pattern of hyperlipidemic acute pancreatitis based on Chinese Medicine Inheritance Assistant Platform software,hope to offer ideas for the treatment of the disease.Methods:Inpatient c...Objective:To excavate the medical pattern of hyperlipidemic acute pancreatitis based on Chinese Medicine Inheritance Assistant Platform software,hope to offer ideas for the treatment of the disease.Methods:Inpatient cases admitted to the Department of Gastroenterology of Shenzhen Hospital of Traditional Chinese Medicine for HLAP from September 2013 to June 2020 were collected,information on evidence patterns and formulations was extracted,an Excel database was established and professional terms were standardized,and data mining methods such as association rules and entropy clustering of complex systems were used to analyze medical patterns.Results:The main symbols of HLAP include abdominal pain,abdominal distinction,vomiting,fever,etc.The common used drags include Immature Orange Fruit,Baical Skullcap Root,Rhubarb and Chinese Thorowax Root,etc.The main drug pairs based on association rule analysis are"Baical Skullcap Root,Immature Orange Fruit","Rhubarb,Immature Orange Fruit","Baical Skullcap Root,Rhubarb,Immature Orange Fruit",etc,and 14 drug core combinations and 7 new formulations were extracted.Conclusion:The main synthesis of HLAP are solid-heat knots in the internal organs and dam-heat in the liver and gallbladder,and the formula is based on Da-chai-hu Decoction,the clinical treatment is based on attacking stagnation,activating blood circulation and removing dampness,which can offer reference for the clinical treatment of the disease.展开更多
文摘目的:将脐带华通胶间充质干细胞(WJMSCs)移植到子宫切口瘢痕缺陷(PCSD)模型大鼠体内,探讨其治疗效果。方法:取雌性SD大鼠50只、将其随机分为3组:正常手术组(N-O组)10只、模型对照组(M-C组)20只和模型治疗组(M-T组)20只。孕第19天N-O组剖宫取胎后连续缝合子宫切口;M-C组与M-T组剖宫产术后子宫肌壁注射脂多糖(LPS)联合电凝(功率为10 W)子宫切缘,间断缝合切口。M-T组分A、B亚组(各10只),A组静脉注射0.5 m L+阴道灌注0.5 m L(1×10~9/L)WJMSCs,B组阴道灌注1 m L同浓度WJMSCs,每周1次,共3次;M-C亦分A、B亚组(各10只),与M-T组同样方法处理,仅将生理盐水替代WJMSCs。各组治疗后第1和4周,分别处死6和4只,留双侧子宫进行HE染色、Masson染色、肌动蛋白(actin)和细胞角蛋白免疫组化染色,通过体视学分析,检测子宫肌层和内膜的修复情况。结果:WJMSCs治疗后第1和4周,M-C组子宫肌层厚度和actin体积密度(Vv)均显著低于N-O组,肌层胶原纤维Vv和内膜缺陷率高于N-O组(P<0.05);WJMSCs治疗后第1和4周,M-T的A和B组的肌层厚度和actin Vv均显著高于M-C组,肌层胶原纤维Vv低于M-C组;M-C组内膜缺陷率高于其它组(P<0.05)。结论:WJMSCs可促进PCSD大鼠子宫肌层结构和功能的修复。静脉+局部联合治疗和单纯阴道治疗效果无明显差别。
文摘利用大肠埃希菌(E.coli O111:B4)中提取精制的内毒素(Control standard endotoxin, CSE)为研究对象,以MnO2/石墨烯(MnO2/G)纳米复合材料为基质,建立了一种基于MnO2/G纳米材料的表面辅助激光解吸电离质谱(Surface-assisted laser desorption ionization mass spectrometry,SALDI-MS)的内毒素检测新方法.利用SALDI-MS方法可实现对不同注射液和饮用水中内毒素的快速鉴定与定量分析.与传统的鲎试剂检测方法相比,基于MnO2/G纳米复合材料的SALDI-MS方法具有操作简便、灵敏度高、分辨率高、检测速度快、高通量和耐盐性好等优点,有望应用于更多食品和药品中细菌内毒素的高通量快速筛查.
基金Huang Bin Guangdong Famous Chinese Medicine Studio(Guangdong Chinese Medicine Office Letter[2020]No.1)。
文摘Objective:To excavate the medical pattern of hyperlipidemic acute pancreatitis based on Chinese Medicine Inheritance Assistant Platform software,hope to offer ideas for the treatment of the disease.Methods:Inpatient cases admitted to the Department of Gastroenterology of Shenzhen Hospital of Traditional Chinese Medicine for HLAP from September 2013 to June 2020 were collected,information on evidence patterns and formulations was extracted,an Excel database was established and professional terms were standardized,and data mining methods such as association rules and entropy clustering of complex systems were used to analyze medical patterns.Results:The main symbols of HLAP include abdominal pain,abdominal distinction,vomiting,fever,etc.The common used drags include Immature Orange Fruit,Baical Skullcap Root,Rhubarb and Chinese Thorowax Root,etc.The main drug pairs based on association rule analysis are"Baical Skullcap Root,Immature Orange Fruit","Rhubarb,Immature Orange Fruit","Baical Skullcap Root,Rhubarb,Immature Orange Fruit",etc,and 14 drug core combinations and 7 new formulations were extracted.Conclusion:The main synthesis of HLAP are solid-heat knots in the internal organs and dam-heat in the liver and gallbladder,and the formula is based on Da-chai-hu Decoction,the clinical treatment is based on attacking stagnation,activating blood circulation and removing dampness,which can offer reference for the clinical treatment of the disease.
