Flower color as the important economic indicator for ornamental plants has become the primary target for ornamental improving and breeding.Heavy ion mutation technology as a unique and efficient mutagen has been widel...Flower color as the important economic indicator for ornamental plants has become the primary target for ornamental improving and breeding.Heavy ion mutation technology as a unique and efficient mutagen has been widely used in germplasm innovation and plant breeding.In our study,150 young shoots of Pelargonium were exposed to 80 MeV/u carbon ions and finally one stable genetic flower color mutant was obtained at dosage of 30 Gy.Comparing with wild type,the physiological indexes of mutant displayed significant differences.The color of petal in mutant was changed from red to light pink,and the color of peduncle and torus was changed from green to red,as well as the pistils and stamens’color were changed from yellow green to purplish red.展开更多
Since the whole genome sequence of Arabidopsis thaliana ecotype Columbia (Col-0) was published in 2 000, the focus of genomics study had turned from structural genomics to functional genomics era[1]. In the post genom...Since the whole genome sequence of Arabidopsis thaliana ecotype Columbia (Col-0) was published in 2 000, the focus of genomics study had turned from structural genomics to functional genomics era[1]. In the post genomics era, mutants play an important role for gene function exploring. Therefore, acquiring various variants and studying on them are useful for understanding the mechanism of plant development and molecular design for plant breeding.展开更多
To investigate the DNA polymorphism of M2 plants of Lotus japonicus induced by heavy ion beam, dry seeds were irradiated by carbon ion beams accelerated by Heavy Ion Research Facility in Lanzhou (HIRFL). Inter simple ...To investigate the DNA polymorphism of M2 plants of Lotus japonicus induced by heavy ion beam, dry seeds were irradiated by carbon ion beams accelerated by Heavy Ion Research Facility in Lanzhou (HIRFL). Inter simple sequence repeat (ISSR) assay were utilized to explore the DNA variation of M2 plants irradiated by 400 Gy carbon ion beams. ISSR technique is an effective molecular marker method based on polymerase chain reaction amplification of template DNA by a single primer with 1626 bp[1]. ISSR can detect the differences of genetic structure of various life materials rapidly.展开更多
Arabidopsis thaliana is one of the most commonly used model plants due to its short life cycle and concise genome.As a novel physical mutagen, heavy ion beams have been widely used in plant breeding. The most signific...Arabidopsis thaliana is one of the most commonly used model plants due to its short life cycle and concise genome.As a novel physical mutagen, heavy ion beams have been widely used in plant breeding. The most significant advantages of heavy ion beams are the higher linear energy transfer (LET) and relative biological effectiveness (RBE), inducing a broad spectrum of mutation phenotypes even at low irradiation[1]. In order to investigate the effects on DNA behavior of Arabidopsis thaliana mutants induced by carbon ion irradiations, inter-simple sequence repeat (ISSR), a DNA-based molecular marker technique, was performed to assess the genetic polymorphism.展开更多
In recent years,heavy ion beams have been recognized as an effective and efficient physical mutagen due to their capacity to induce mutations with high frequency and broad spectrum.Nowadays,it is not so difficult to p...In recent years,heavy ion beams have been recognized as an effective and efficient physical mutagen due to their capacity to induce mutations with high frequency and broad spectrum.Nowadays,it is not so difficult to produce various mutants in plants.However,to mine the responsible mutated genes has been an important challenge.For mutation isolation,map-based cloning is one of the major traditional ways to isolate the mutant genes that control traits of interest in forward genetics studies.However,the process of map-based cloning is usually complicated and time-consuming.展开更多
In recent years,with the development of robotics,sensors and kinds of imaging apparatuses,researchers have developed varieties of automation,high precision and throughput phenotype analysis platforms by ceaselessly ha...In recent years,with the development of robotics,sensors and kinds of imaging apparatuses,researchers have developed varieties of automation,high precision and throughput phenotype analysis platforms by ceaselessly hard working and exploring[1,2].Although these platforms have improved the development of phenomics to some degree,there are still some problems in need of resolution.For example,researchers have not yet carried out an effective evaluation of the relationship between plant phenotype and genotype.Certainly,it is the key to solve these problems that how to analyze thoroughly and availably the“big data”obtained from the“high throughput”of the phenotypic analysis platform[3].Based on these issues,we attempted to analyze the huge amounts of data produced by Scanalyzer HTS,and summed up a set of research methods to analyze phenomics data.展开更多
目的系统整合癌症患者及照护者有效疼痛管理障碍的质性研究,为患者及照护者安全有效地实施癌症疼痛管理提供参考。方法计算机检索PubMed、Web of Science、Cochrane Library、Embase、CINAHL、中国知网、中国生物医学文献数据库、维普...目的系统整合癌症患者及照护者有效疼痛管理障碍的质性研究,为患者及照护者安全有效地实施癌症疼痛管理提供参考。方法计算机检索PubMed、Web of Science、Cochrane Library、Embase、CINAHL、中国知网、中国生物医学文献数据库、维普数据库、万方数据库中收录的关于癌症患者及照护者参与癌症疼痛管理时经历和障碍的质性研究,检索时限为建库至2023年9月,根据澳大利亚乔安娜布里格斯研究所循证卫生保健中心质性研究质量评价标准评价文献质量。采用汇集性整合进行Meta整合。结果共纳入9篇文献,提炼出46个研究结果,形成14个类别,进一步归纳为4个整合结果:与患者相关的疼痛管理障碍、与照护者相关的疼痛管理障碍、与医护人员相关的疼痛管理障碍、与医疗保健系统相关的疼痛管理障碍。结论多方因素造成癌症患者及照护者参与疼痛管理存在障碍,医护人员应深入了解患者的疼痛困扰,探索适用于癌症患者的疼痛管理计划和干预措施,从而降低癌症疼痛管理障碍程度。展开更多
文摘Flower color as the important economic indicator for ornamental plants has become the primary target for ornamental improving and breeding.Heavy ion mutation technology as a unique and efficient mutagen has been widely used in germplasm innovation and plant breeding.In our study,150 young shoots of Pelargonium were exposed to 80 MeV/u carbon ions and finally one stable genetic flower color mutant was obtained at dosage of 30 Gy.Comparing with wild type,the physiological indexes of mutant displayed significant differences.The color of petal in mutant was changed from red to light pink,and the color of peduncle and torus was changed from green to red,as well as the pistils and stamens’color were changed from yellow green to purplish red.
文摘Since the whole genome sequence of Arabidopsis thaliana ecotype Columbia (Col-0) was published in 2 000, the focus of genomics study had turned from structural genomics to functional genomics era[1]. In the post genomics era, mutants play an important role for gene function exploring. Therefore, acquiring various variants and studying on them are useful for understanding the mechanism of plant development and molecular design for plant breeding.
文摘To investigate the DNA polymorphism of M2 plants of Lotus japonicus induced by heavy ion beam, dry seeds were irradiated by carbon ion beams accelerated by Heavy Ion Research Facility in Lanzhou (HIRFL). Inter simple sequence repeat (ISSR) assay were utilized to explore the DNA variation of M2 plants irradiated by 400 Gy carbon ion beams. ISSR technique is an effective molecular marker method based on polymerase chain reaction amplification of template DNA by a single primer with 1626 bp[1]. ISSR can detect the differences of genetic structure of various life materials rapidly.
文摘Arabidopsis thaliana is one of the most commonly used model plants due to its short life cycle and concise genome.As a novel physical mutagen, heavy ion beams have been widely used in plant breeding. The most significant advantages of heavy ion beams are the higher linear energy transfer (LET) and relative biological effectiveness (RBE), inducing a broad spectrum of mutation phenotypes even at low irradiation[1]. In order to investigate the effects on DNA behavior of Arabidopsis thaliana mutants induced by carbon ion irradiations, inter-simple sequence repeat (ISSR), a DNA-based molecular marker technique, was performed to assess the genetic polymorphism.
文摘In recent years,heavy ion beams have been recognized as an effective and efficient physical mutagen due to their capacity to induce mutations with high frequency and broad spectrum.Nowadays,it is not so difficult to produce various mutants in plants.However,to mine the responsible mutated genes has been an important challenge.For mutation isolation,map-based cloning is one of the major traditional ways to isolate the mutant genes that control traits of interest in forward genetics studies.However,the process of map-based cloning is usually complicated and time-consuming.
文摘In recent years,with the development of robotics,sensors and kinds of imaging apparatuses,researchers have developed varieties of automation,high precision and throughput phenotype analysis platforms by ceaselessly hard working and exploring[1,2].Although these platforms have improved the development of phenomics to some degree,there are still some problems in need of resolution.For example,researchers have not yet carried out an effective evaluation of the relationship between plant phenotype and genotype.Certainly,it is the key to solve these problems that how to analyze thoroughly and availably the“big data”obtained from the“high throughput”of the phenotypic analysis platform[3].Based on these issues,we attempted to analyze the huge amounts of data produced by Scanalyzer HTS,and summed up a set of research methods to analyze phenomics data.
文摘目的系统整合癌症患者及照护者有效疼痛管理障碍的质性研究,为患者及照护者安全有效地实施癌症疼痛管理提供参考。方法计算机检索PubMed、Web of Science、Cochrane Library、Embase、CINAHL、中国知网、中国生物医学文献数据库、维普数据库、万方数据库中收录的关于癌症患者及照护者参与癌症疼痛管理时经历和障碍的质性研究,检索时限为建库至2023年9月,根据澳大利亚乔安娜布里格斯研究所循证卫生保健中心质性研究质量评价标准评价文献质量。采用汇集性整合进行Meta整合。结果共纳入9篇文献,提炼出46个研究结果,形成14个类别,进一步归纳为4个整合结果:与患者相关的疼痛管理障碍、与照护者相关的疼痛管理障碍、与医护人员相关的疼痛管理障碍、与医疗保健系统相关的疼痛管理障碍。结论多方因素造成癌症患者及照护者参与疼痛管理存在障碍,医护人员应深入了解患者的疼痛困扰,探索适用于癌症患者的疼痛管理计划和干预措施,从而降低癌症疼痛管理障碍程度。
