OBJECTIVE:To explore the mechanisms of dorsal root ganglia and spinal microglia cascade cross in electroacupuncture(EA)analgesia in the treatment of lumbar disc herniation.METHODS:A rat model of lumbar disc herniation...OBJECTIVE:To explore the mechanisms of dorsal root ganglia and spinal microglia cascade cross in electroacupuncture(EA)analgesia in the treatment of lumbar disc herniation.METHODS:A rat model of lumbar disc herniation(LDH)was established,EA was administered at Huantiao(GB30)acupoint 30 min once a day,for 3 d.Before and after modeling,and after EA,mechanical allodynia thresholds were detected.Hyperpolarization-activated cyclic nucleotide-gated 2(HCN2)in dorsal root ganglia was detected by quantitative polymerase chain reaction(qPCR)and Western blot.C-X3-C motif chemokine ligand 1(CX3CL1)and activity of microglia in spinal cord was observed separately via qPCR and immunofluorescence staining.RESULTS:The mechanical allodynia threshold of the right planta of model rats was significantly reduced(P<0.01),EA increased the mechanical pain threshold of rats(P<0.01),and decreased HCN2 mRNA,and protein expression,reduced the expression of CX3CL1 and the activation of microglia.ZD7288(a blocker of HCN channel)reduced the analgesic effect of EA from 1.83±0.84 to 0.74±0.20(P<0.05),and the expression of CX3CL1 in the spinal cord decreased from 0.52±0.11 to 0.15±0.05(P<0.01).CONCLUSION:EA analgesia on the radicular pain of LDH is definite.EA reduced the expression of HCN2 channel in the dorsal root ganglion,thereby decreasing the noxious stimulation entered to microglia in spinal dorsal horn.Our work supports EA is an effective treatment for radicular pain of LDH.展开更多
基金Supported by Inheritance and Innovation in TCM“Hundred-Thousand-Ten Thousand”Talent Project(Qinhuang Project)(No.F119090038)the National Natural Science Foundation of China(Based on the Cascade Reaction of Microglia-Astrocyte research exosomal mi RNA mechanisms of the inhibitory transition from acute to chronic pain of LDH by electroacupuncture,No.82074529)Scientific Research Projects of Traditional Chinese Medicine Bureau of Guangdong Province(Based on the cAMP-PKA-HCN2 pathway the mechanism of electro-acupuncture to prevent the development of pain in lumbar disc herniation,No.20211254)
文摘OBJECTIVE:To explore the mechanisms of dorsal root ganglia and spinal microglia cascade cross in electroacupuncture(EA)analgesia in the treatment of lumbar disc herniation.METHODS:A rat model of lumbar disc herniation(LDH)was established,EA was administered at Huantiao(GB30)acupoint 30 min once a day,for 3 d.Before and after modeling,and after EA,mechanical allodynia thresholds were detected.Hyperpolarization-activated cyclic nucleotide-gated 2(HCN2)in dorsal root ganglia was detected by quantitative polymerase chain reaction(qPCR)and Western blot.C-X3-C motif chemokine ligand 1(CX3CL1)and activity of microglia in spinal cord was observed separately via qPCR and immunofluorescence staining.RESULTS:The mechanical allodynia threshold of the right planta of model rats was significantly reduced(P<0.01),EA increased the mechanical pain threshold of rats(P<0.01),and decreased HCN2 mRNA,and protein expression,reduced the expression of CX3CL1 and the activation of microglia.ZD7288(a blocker of HCN channel)reduced the analgesic effect of EA from 1.83±0.84 to 0.74±0.20(P<0.05),and the expression of CX3CL1 in the spinal cord decreased from 0.52±0.11 to 0.15±0.05(P<0.01).CONCLUSION:EA analgesia on the radicular pain of LDH is definite.EA reduced the expression of HCN2 channel in the dorsal root ganglion,thereby decreasing the noxious stimulation entered to microglia in spinal dorsal horn.Our work supports EA is an effective treatment for radicular pain of LDH.
