Genetic analysis of rolled leaf is important to rice ideotype breeding. To detect loci controlling rolled leaf of japonica restorer lines, SSR marker genotypes and phenotypes of flag leaf rolling index （LRI） were in...Genetic analysis of rolled leaf is important to rice ideotype breeding. To detect loci controlling rolled leaf of japonica restorer lines, SSR marker genotypes and phenotypes of flag leaf rolling index （LRI） were investigated in Xiushui 79 （P1, a japonica rice variety）, C Bao （P2, a japonica restorer line） and 254 recombinant inbred lines derived from the cross between P1 and P2 , and in two environments. A genetic map of this cross was constructed, QTLs for LRI were detected and their interactions with environments were analyzed. Among 818 pairs of SSR primers, 90 primers showed polymorphism between P1 and P2, and 12 markers showed highly significant correlation with LRI in both environments based on single marker regression analysis. The genetic map containing 74 information loci has a total distance of 744.6 cM, with an average of 10.1 cM between two adjacent loci. Three QTLs （qRL-1, qRL-7 and qRL-8-1） were detected with two softwares： WinQTLCart 2.5 and QTLNetwork2.0. qRL-8-1 was a new locus, accounting for 15.5% and 12.8% of phenotypic variations in the two environments, respectively. The phenotypic variation explained by additive effect was 6.6%. No interaction was found between qRL-8-1 genotype and environments.展开更多
目的评价高血压患者在睡前与晨起服用小剂量阿司匹林对血压的影响。方法通过计算机检索中国知网、维普数据库、中国生物医学文献数据库、万方数据库、Cochrane Library、Pubmed、Web of Science数据库,检索时限至2018年10月,按照纳入和...目的评价高血压患者在睡前与晨起服用小剂量阿司匹林对血压的影响。方法通过计算机检索中国知网、维普数据库、中国生物医学文献数据库、万方数据库、Cochrane Library、Pubmed、Web of Science数据库,检索时限至2018年10月,按照纳入和排除标准由2名评价者独立筛选并提取资料,采用Cochrane 5.1手册提供的偏椅风险评估方法,对纳入研究进行治疗评价后,采用RevMan 5.3软件进行Meta分析。结果共纳入7篇文献,共1102例。Meta分析结果显示:睡前与晨起服用小剂量阿司匹林对收缩压的影响,进行异质性检验结果示(χ~2=57.50,P<0.00001,I^2=90%)。采用随机效应模型,结果示(MD-3.74,95%CI(-7.11,-0.38),Z=2.18,P=0.03)。剔除丘文山^([7])、董军亚^([8])、廖承广^([9])等研究后,采用固定效应模型进行Meta分析,结果示(MD-2.09,95%CI(-3.84,-0.33),Z=2.33,P=0.02),睡前服用小剂量阿司匹林降低高血压患者收缩压优于晨起服用(P<0.05);睡前与晨起服用小剂量阿司匹林对舒张压的影响,进行异质性检验结果示(χ~2=40.21,P<0.00001,I^2=85%),采用随机效应模型,结果示(MD-2.63,95%CI(-4.71,-0.55),Z=2.48,P=0.01)。剔除董军亚^([8])、廖承广^([9])研究后,采用固定效应模型进行分析,睡前与晨起服用小剂量阿司匹林对高血压患者舒张压影响比较,差异无统计学意义(MD-0.85,95%CI(-1.91,0.21),Z=1.57,P=0.12)。结论睡前服用小剂量阿司匹林较晨起服用可能降低高血压患者的收缩压,尚不能确定是否降低舒张压。鉴于研究样本量少及部分文献质量限制,论证强度受到一定限制,因此尚需进一步开展高质量、大样本随机对照试验对本研究结果予以验证,从而为临床提供更可靠的证据。展开更多
基金supported by the Program of Introducing Talents of Discipline to University in China (Grant No.B08025)
文摘Genetic analysis of rolled leaf is important to rice ideotype breeding. To detect loci controlling rolled leaf of japonica restorer lines, SSR marker genotypes and phenotypes of flag leaf rolling index （LRI） were investigated in Xiushui 79 （P1, a japonica rice variety）, C Bao （P2, a japonica restorer line） and 254 recombinant inbred lines derived from the cross between P1 and P2 , and in two environments. A genetic map of this cross was constructed, QTLs for LRI were detected and their interactions with environments were analyzed. Among 818 pairs of SSR primers, 90 primers showed polymorphism between P1 and P2, and 12 markers showed highly significant correlation with LRI in both environments based on single marker regression analysis. The genetic map containing 74 information loci has a total distance of 744.6 cM, with an average of 10.1 cM between two adjacent loci. Three QTLs （qRL-1, qRL-7 and qRL-8-1） were detected with two softwares： WinQTLCart 2.5 and QTLNetwork2.0. qRL-8-1 was a new locus, accounting for 15.5% and 12.8% of phenotypic variations in the two environments, respectively. The phenotypic variation explained by additive effect was 6.6%. No interaction was found between qRL-8-1 genotype and environments.
