"Hu Huan-yong Line(Hu Line)"depicts a geographical pattern of China’s population distribution.Its essence is the regionality of humanland relationship and reflects basic characteristics and laws of human be..."Hu Huan-yong Line(Hu Line)"depicts a geographical pattern of China’s population distribution.Its essence is the regionality of humanland relationship and reflects basic characteristics and laws of human beings’adaptation to the natural environment.With the development of the times and the progress of modern science and technology,especially the rapid urbanization and construction of transportation network system in China,the connection between economic and geographical space has been continuously strengthened.The geographical transition zones from mountain areas to plains,i.e.,transitional geographical space,have promoted the changes in human-land relationships through population migration and agglomeration.Taking Sichuan-Yunnan provinces at the southern end of Hu Line as study area,this study analyzed the spatial correlation between population distribution and economy in this region,explored the pattern of geographical agglomeration and deagglomeration,and explained the changing characteristics of humanland relationship in transitional geographic space using global Moran’s I index,global regression model(GRM)and geographically weighted regression(GWR).The results show that population and Gross Domestic Product(GDP)have significant spatial dependence to this region,with obvious aggregation in geographical distribution and positive autocorrelation;comparing with the general least square model,the GWR model incorporating spatial effect was more suitable for revealing the distribution characteristics of geographical elements,with fine results and better fitting;the spatial model of population and GDP as well as the spatio-temporal evolution model of their changes,all of them strongly indicated that Normalized Difference Vegetation Index(NDVI)and road density were important factors governing the spatial differentiation of population and economy;under the rapid development of regional economy and continuous evolution of urban-rural relations,rural transformation and spatial reconstruction promoted the change of population migration and agglomeration.展开更多
OBJECTIVE To examine whether long-form phosphodiesterase-4(PDE4)knockdown by lentiviral RNA construct containing a speci fi c micro RNA/mi RNAmir hairpin structure reversed depression-like symptoms caused by chronic u...OBJECTIVE To examine whether long-form phosphodiesterase-4(PDE4)knockdown by lentiviral RNA construct containing a speci fi c micro RNA/mi RNAmir hairpin structure reversed depression-like symptoms caused by chronic unpredictable mild stress(CUMS)in mice.METHODS In this research,the study was performed on adult male C57 mice,weighing(25±5)g,kept in a controlled environment.CUMS animal model was used recapitulate a multiple of behavioral characteristics and biochemical states of depression in human.The forced swimming test(FST)and the tail suspension test(TST)were used to detectthe state of depression.Western blotting analysis was used to assess protein levels of c AMP response element binding protein(CREB,unphosphorylated and phosphorylated[p CREB])to explore the neurochemical mechanisms.RESULTS CUMS decreased c AMP levels(P<0.01)and produced depression-like symptoms in FST(P<0.01)and TST(P<0.01).Microinfusions of lentiviruses reversed CUMS-induced c AMP decline(P<0.05)and depression-like symptoms.Moreover,CUMS caused a significant reduction in protein kinase A and CREB phosphorylation,and brain-derived neurotrophic factor transcription,both of which were partially attenuated by lentivirus-mediated knockdown of PDE4D.Also,the phosphorylation of extracellular signal-regulated kinase 1/2 was reduced in CUMS-exposed mice,which was reversed by 4Dmi RNA treatment.Taken together,this study demonstrated that PDE4Dmi RNA improved the CUMS-induced depressionlike symptoms that might be related to the increase in hippocampal c AMP and p CREB expression.CONCLUSION Hence,PDE4D inhibitors can serve as potential antidepressants,and their antidepressant activity is partially mediated by the activation of c AMP signaling pathway in the hippocampus.In other words,long-form PDE4D knockdown may offer a promising treatment for major depression disorder.展开更多
A 2D layer Cu(Ⅱ) coordination polymer [Cu(npth)(H_(2)O)]n(1) was crystallized from a mixture of 3-nitrophthalic acid and Cu(OAc)_(2)·H_(2)O in water under room temperature and structurally characterized by singl...A 2D layer Cu(Ⅱ) coordination polymer [Cu(npth)(H_(2)O)]n(1) was crystallized from a mixture of 3-nitrophthalic acid and Cu(OAc)_(2)·H_(2)O in water under room temperature and structurally characterized by single-crystal X-ray diffraction, FT-IR and TGA. Compound 1 was applied to make a nanocomposite with graphene oxide(GO). A highly dispersible and stable nanocomposite of Cu(npth)-GO was successfully synthesized by a simple ultrasonication method. SEM, TEM, UV-vis, FT-IR and TGA were used to characterize the morphology and structure of the prepared composite. In accordance with the characterization results, we suspected that the binding mechanism of Cu(npth) and GO was assigned to be the cooperative interaction of Cu–O coordination, π-π stacking and hydrogen bonding.展开更多
基金funded by the National Natural Science Foundation of China(41930651,41971226,41871357)Science and Technology Service Network Program(STS)Project of Chinese Academy of Sciences(Y8R2020022)。
文摘"Hu Huan-yong Line(Hu Line)"depicts a geographical pattern of China’s population distribution.Its essence is the regionality of humanland relationship and reflects basic characteristics and laws of human beings’adaptation to the natural environment.With the development of the times and the progress of modern science and technology,especially the rapid urbanization and construction of transportation network system in China,the connection between economic and geographical space has been continuously strengthened.The geographical transition zones from mountain areas to plains,i.e.,transitional geographical space,have promoted the changes in human-land relationships through population migration and agglomeration.Taking Sichuan-Yunnan provinces at the southern end of Hu Line as study area,this study analyzed the spatial correlation between population distribution and economy in this region,explored the pattern of geographical agglomeration and deagglomeration,and explained the changing characteristics of humanland relationship in transitional geographic space using global Moran’s I index,global regression model(GRM)and geographically weighted regression(GWR).The results show that population and Gross Domestic Product(GDP)have significant spatial dependence to this region,with obvious aggregation in geographical distribution and positive autocorrelation;comparing with the general least square model,the GWR model incorporating spatial effect was more suitable for revealing the distribution characteristics of geographical elements,with fine results and better fitting;the spatial model of population and GDP as well as the spatio-temporal evolution model of their changes,all of them strongly indicated that Normalized Difference Vegetation Index(NDVI)and road density were important factors governing the spatial differentiation of population and economy;under the rapid development of regional economy and continuous evolution of urban-rural relations,rural transformation and spatial reconstruction promoted the change of population migration and agglomeration.
基金The project supported by National Natural Science Foundation of China(81301099,81373384)Natural Science Foundation of Guangdong Province(S2013040014202)+1 种基金China Postdoctoral Science Foundation(2013M542192)National Science and Technology Major Projects for"Major New Drugs Innovation and Development"(2012ZX09J1211003C)
文摘OBJECTIVE To examine whether long-form phosphodiesterase-4(PDE4)knockdown by lentiviral RNA construct containing a speci fi c micro RNA/mi RNAmir hairpin structure reversed depression-like symptoms caused by chronic unpredictable mild stress(CUMS)in mice.METHODS In this research,the study was performed on adult male C57 mice,weighing(25±5)g,kept in a controlled environment.CUMS animal model was used recapitulate a multiple of behavioral characteristics and biochemical states of depression in human.The forced swimming test(FST)and the tail suspension test(TST)were used to detectthe state of depression.Western blotting analysis was used to assess protein levels of c AMP response element binding protein(CREB,unphosphorylated and phosphorylated[p CREB])to explore the neurochemical mechanisms.RESULTS CUMS decreased c AMP levels(P<0.01)and produced depression-like symptoms in FST(P<0.01)and TST(P<0.01).Microinfusions of lentiviruses reversed CUMS-induced c AMP decline(P<0.05)and depression-like symptoms.Moreover,CUMS caused a significant reduction in protein kinase A and CREB phosphorylation,and brain-derived neurotrophic factor transcription,both of which were partially attenuated by lentivirus-mediated knockdown of PDE4D.Also,the phosphorylation of extracellular signal-regulated kinase 1/2 was reduced in CUMS-exposed mice,which was reversed by 4Dmi RNA treatment.Taken together,this study demonstrated that PDE4Dmi RNA improved the CUMS-induced depressionlike symptoms that might be related to the increase in hippocampal c AMP and p CREB expression.CONCLUSION Hence,PDE4D inhibitors can serve as potential antidepressants,and their antidepressant activity is partially mediated by the activation of c AMP signaling pathway in the hippocampus.In other words,long-form PDE4D knockdown may offer a promising treatment for major depression disorder.
基金Supported by the National Natural Science Foundation of China (No.22001064)the Natural Science Foundation of Hunan Province (No.2020JJ4155)+1 种基金the 2020 Hunan Province College Students’Innovation Entrepreneurship Training Program (No.3373)the Scientific Research Project of Hunan Province Department of Education (No.20B105)。
文摘A 2D layer Cu(Ⅱ) coordination polymer [Cu(npth)(H_(2)O)]n(1) was crystallized from a mixture of 3-nitrophthalic acid and Cu(OAc)_(2)·H_(2)O in water under room temperature and structurally characterized by single-crystal X-ray diffraction, FT-IR and TGA. Compound 1 was applied to make a nanocomposite with graphene oxide(GO). A highly dispersible and stable nanocomposite of Cu(npth)-GO was successfully synthesized by a simple ultrasonication method. SEM, TEM, UV-vis, FT-IR and TGA were used to characterize the morphology and structure of the prepared composite. In accordance with the characterization results, we suspected that the binding mechanism of Cu(npth) and GO was assigned to be the cooperative interaction of Cu–O coordination, π-π stacking and hydrogen bonding.
