In the pilot study of treating the purified terephthalic acid(PTA) wastewater with the functional Strain Fhhh in the carrier activated sludge process(CASP), the ratio of COD∶TN∶TP and the concentrations of Cu, Mn, S...In the pilot study of treating the purified terephthalic acid(PTA) wastewater with the functional Strain Fhhh in the carrier activated sludge process(CASP), the ratio of COD∶TN∶TP and the concentrations of Cu, Mn, Se and Zn were controlled to improve the manganese peroxidase(MnP) levels for increasing the treatment efficiency. When the ratio of COD∶TN∶TP was 100∶0.36∶0.15 and the concentrations of Cu, Mn, Se and Zn were 0.54, 5.07, 0.00 and 0.08 mg/L, the MnP specific activity(MnP-SA) reached 689 U/L, and the sludge loading rate to COD(SLRC) was 1.09 d -1, which was 4—7 fold of that in other processes reported. The data indicated that improving MnP level could enhance the degradability of Fhhh. And the potentials of Fhhh and CASP will be also discussed in this paper.展开更多
There were 6 target DNA fragments of the three parental strains existing in the cell of GEMs(genetically engineered microorganism strain) Fhhh measured in this research by PCR(polymerase chain reaction). The determina...There were 6 target DNA fragments of the three parental strains existing in the cell of GEMs(genetically engineered microorganism strain) Fhhh measured in this research by PCR(polymerase chain reaction). The determination showed that GEMs Fhhh contained all the 6 target DNA fragments, mnp 1, mnp 2、 lip 1、 lip 2, FLO 1 and 16S rDNA, and had the molecular genetic stability. Meanwhile the PCR production of each parental strain could only had its target DNA fragments and was different from each other. It may illustrate that the technique of the inter kingdom protoplast fusion for the construction of GEMs Fhhh through the process of intercellular gene recombination could be used as a reliable bioengineering technique to create the specific functional stain for the pollution control.展开更多
基金Foundation item: The National Hi Tech Research and Development Program(863) of China(2001AA214191) and PhD Fund of the National Education Ministry of China(No. 20030284038)
文摘In the pilot study of treating the purified terephthalic acid(PTA) wastewater with the functional Strain Fhhh in the carrier activated sludge process(CASP), the ratio of COD∶TN∶TP and the concentrations of Cu, Mn, Se and Zn were controlled to improve the manganese peroxidase(MnP) levels for increasing the treatment efficiency. When the ratio of COD∶TN∶TP was 100∶0.36∶0.15 and the concentrations of Cu, Mn, Se and Zn were 0.54, 5.07, 0.00 and 0.08 mg/L, the MnP specific activity(MnP-SA) reached 689 U/L, and the sludge loading rate to COD(SLRC) was 1.09 d -1, which was 4—7 fold of that in other processes reported. The data indicated that improving MnP level could enhance the degradability of Fhhh. And the potentials of Fhhh and CASP will be also discussed in this paper.
文摘There were 6 target DNA fragments of the three parental strains existing in the cell of GEMs(genetically engineered microorganism strain) Fhhh measured in this research by PCR(polymerase chain reaction). The determination showed that GEMs Fhhh contained all the 6 target DNA fragments, mnp 1, mnp 2、 lip 1、 lip 2, FLO 1 and 16S rDNA, and had the molecular genetic stability. Meanwhile the PCR production of each parental strain could only had its target DNA fragments and was different from each other. It may illustrate that the technique of the inter kingdom protoplast fusion for the construction of GEMs Fhhh through the process of intercellular gene recombination could be used as a reliable bioengineering technique to create the specific functional stain for the pollution control.