Two transcripts of T gene, T1170 and T1243, were obtained from the mitochondrial cDNA of tuber mus- tard CMS line. T1243 was a transcript with an intron unspliced, which has the basic characteristics of type Ⅱ intron...Two transcripts of T gene, T1170 and T1243, were obtained from the mitochondrial cDNA of tuber mus- tard CMS line. T1243 was a transcript with an intron unspliced, which has the basic characteristics of type Ⅱ intron. The expressions of the two transcripts were analyzed by reverse transcription PCR (RT-PCR). The results showed that, at seedling stage, the expression of T gene was mainly in the form of T1170 but decreased with the development gradually, while the expression abundance of another tran- script, T1243, increased gradually. The T1243 was prevalent at the profuse flowering stage. The expression pattern was confirmed by Northern blot analysis. These results suggested that the alternative spliced mitochondrial T gene transcripts were related to CMS of tuber mustard.展开更多
文摘Two transcripts of T gene, T1170 and T1243, were obtained from the mitochondrial cDNA of tuber mus- tard CMS line. T1243 was a transcript with an intron unspliced, which has the basic characteristics of type Ⅱ intron. The expressions of the two transcripts were analyzed by reverse transcription PCR (RT-PCR). The results showed that, at seedling stage, the expression of T gene was mainly in the form of T1170 but decreased with the development gradually, while the expression abundance of another tran- script, T1243, increased gradually. The T1243 was prevalent at the profuse flowering stage. The expression pattern was confirmed by Northern blot analysis. These results suggested that the alternative spliced mitochondrial T gene transcripts were related to CMS of tuber mustard.
