目的运用生物信息学分析铜死亡CDKN2A基因在肺腺癌中的表达水平、预后价值、免疫细胞浸润、相关基因筛选、功能富集分析及潜在中药预测,以期为临床治疗提供参考。方法使用GEPIA2和UALCAN,研究CDKN2A在肺腺癌患者中的表达情况,研究它与...目的运用生物信息学分析铜死亡CDKN2A基因在肺腺癌中的表达水平、预后价值、免疫细胞浸润、相关基因筛选、功能富集分析及潜在中药预测,以期为临床治疗提供参考。方法使用GEPIA2和UALCAN,研究CDKN2A在肺腺癌患者中的表达情况,研究它与患者的年龄、性别和种族等之间存在的相关性;使用KM-plotter来评估预后价值;使用TIMER 2.0进行免疫浸润相关性分析;利用LinkedOmics数据库筛选相关基因,进行功能富集分析;利用COREMINE数据库进行中药预测,TCMSP数据库、HERB数据库及Swiss ADME平台筛选出上述中药有效活性成分,Cytoscape软件绘制“中药-活性成分”网络图。结果按照P<0.05检验水平,CDKN2A在肺腺癌组织中显著高表达,与患者吸烟、种族、TP53突变有关,与年龄、性别、肿瘤分级、肿瘤分期无关。CDKN2A高表达患者预后差(HR=1.73;P=0.00024)。CDKN2A与多种免疫细胞浸润相关。基因本体(gene ontology,GO)富集分析显示正相关基因主要参与细胞周期、ncRNA 3′-末端处理、核酸磷酸二酯键水解过程,表达于含细胞蛋白复合物、染色体组织,基因组百科全书(Kyoto encyclopedia of genes and genomes,KEGG)通路分析显示正相关基因参与细胞周期与DNA复制过程。预测出虎刺、蜂房、兰花根(春兰根)等9味中药,其四气以温、平为主,五味以甘、辛、苦为主,归经主要归肝、肺、脾经,有效活性成分有73种。结论CDKN2A在肺腺癌组织中显著高表达,高表达患者预后差。虎刺、蜂房、兰花根等9味中药可能是治疗CDKN2A基因突变的肺腺癌患者的潜在药物。展开更多
Silica-phosphatic nodules are abundant in black shales of the Yanjiahe Formation in the Three Gorges Area of South China,which is correlated to the Fortunian Stage,Terreneuvian Series,Cambrian System.The nodules are r...Silica-phosphatic nodules are abundant in black shales of the Yanjiahe Formation in the Three Gorges Area of South China,which is correlated to the Fortunian Stage,Terreneuvian Series,Cambrian System.The nodules are rich in small shelly fossils and hence attract the attention of numerous paleontologists and sedimentary geologists.However,the genesis of the nodules and the preservation of the small shelly fossils are poorly understood.Here we analyze morphological,structural,mineralogical and chemical features of the nodules in multiscale using a combination of micro-X-ray fluorescence spectroscopy,X-ray diffraction,scanning electron microscopy and confocal laser Raman spectroscopy.Results reveal that nodules are concentric in chemical and mineralogical compositions,comprising a silica-phosphatic core encrusted sequentially by a phosphatic zone,a siliceous zone and a very thin pyrite outer rim.The black shales hosting the nodules demonstrate a laminated texture of alternating clayey and silty laminae,which were respectively deposited in sulfidic/less sulfidic,high/low production,intense/weak chemical weathering conditions.The phosphogenesis of the nodules resulted in the phosphatization of small shelly fossils,which prevented the fossils from being dissolved during diagenesis,whereas the silica encrustation sealed the fossils within the nodules and thus protected the fossils from alteration by deep burial and surface weathering.展开更多
文摘目的运用生物信息学分析铜死亡CDKN2A基因在肺腺癌中的表达水平、预后价值、免疫细胞浸润、相关基因筛选、功能富集分析及潜在中药预测,以期为临床治疗提供参考。方法使用GEPIA2和UALCAN,研究CDKN2A在肺腺癌患者中的表达情况,研究它与患者的年龄、性别和种族等之间存在的相关性;使用KM-plotter来评估预后价值;使用TIMER 2.0进行免疫浸润相关性分析;利用LinkedOmics数据库筛选相关基因,进行功能富集分析;利用COREMINE数据库进行中药预测,TCMSP数据库、HERB数据库及Swiss ADME平台筛选出上述中药有效活性成分,Cytoscape软件绘制“中药-活性成分”网络图。结果按照P<0.05检验水平,CDKN2A在肺腺癌组织中显著高表达,与患者吸烟、种族、TP53突变有关,与年龄、性别、肿瘤分级、肿瘤分期无关。CDKN2A高表达患者预后差(HR=1.73;P=0.00024)。CDKN2A与多种免疫细胞浸润相关。基因本体(gene ontology,GO)富集分析显示正相关基因主要参与细胞周期、ncRNA 3′-末端处理、核酸磷酸二酯键水解过程,表达于含细胞蛋白复合物、染色体组织,基因组百科全书(Kyoto encyclopedia of genes and genomes,KEGG)通路分析显示正相关基因参与细胞周期与DNA复制过程。预测出虎刺、蜂房、兰花根(春兰根)等9味中药,其四气以温、平为主,五味以甘、辛、苦为主,归经主要归肝、肺、脾经,有效活性成分有73种。结论CDKN2A在肺腺癌组织中显著高表达,高表达患者预后差。虎刺、蜂房、兰花根等9味中药可能是治疗CDKN2A基因突变的肺腺癌患者的潜在药物。
基金financially supported by the National Natural Science Foundation of China(grant nos.41890843 and 41920104009)the Innovation Group Project of Southern Marine Science and Engineering Guangdong Laboratory(Zhuhai)(Grant No.311021003)。
文摘Silica-phosphatic nodules are abundant in black shales of the Yanjiahe Formation in the Three Gorges Area of South China,which is correlated to the Fortunian Stage,Terreneuvian Series,Cambrian System.The nodules are rich in small shelly fossils and hence attract the attention of numerous paleontologists and sedimentary geologists.However,the genesis of the nodules and the preservation of the small shelly fossils are poorly understood.Here we analyze morphological,structural,mineralogical and chemical features of the nodules in multiscale using a combination of micro-X-ray fluorescence spectroscopy,X-ray diffraction,scanning electron microscopy and confocal laser Raman spectroscopy.Results reveal that nodules are concentric in chemical and mineralogical compositions,comprising a silica-phosphatic core encrusted sequentially by a phosphatic zone,a siliceous zone and a very thin pyrite outer rim.The black shales hosting the nodules demonstrate a laminated texture of alternating clayey and silty laminae,which were respectively deposited in sulfidic/less sulfidic,high/low production,intense/weak chemical weathering conditions.The phosphogenesis of the nodules resulted in the phosphatization of small shelly fossils,which prevented the fossils from being dissolved during diagenesis,whereas the silica encrustation sealed the fossils within the nodules and thus protected the fossils from alteration by deep burial and surface weathering.
