A Blockchain and CP-ABE Based Access Control Scheme with Fine-Grained Revocation of Attributes in Cloud Health

The Access control scheme is an effective method to protect user data privacy.The access control scheme based on blockchain and ciphertext policy attribute encryption(CP–ABE)can solve the problems of single—point of failure and lack of trust in the centralized system.However,it also brings new problems to the health information in the cloud storage environment,such as attribute leakage,low consensus efficiency,complex permission updates,and so on.This paper proposes an access control scheme with fine-grained attribute revocation,keyword search,and traceability of the attribute private key distribution process.Blockchain technology tracks the authorization of attribute private keys.The credit scoring method improves the Raft protocol in consensus efficiency.Besides,the interplanetary file system(IPFS)addresses the capacity deficit of blockchain.Under the premise of hiding policy,the research proposes a fine-grained access control method based on users,user attributes,and file structure.It optimizes the data-sharing mode.At the same time,Proxy Re-Encryption(PRE)technology is used to update the access rights.The proposed scheme proved to be secure.Comparative analysis and experimental results show that the proposed scheme has higher efficiency and more functions.It can meet the needs of medical institutions.

TiO_(2)/TiN纳米管异质结用于光电催化CO_(2)还原:氮辅助活性氢机制

利用太阳能将CO_(2)转换为高附加值的化学品是解决化石燃料消耗过快与CO_(2)排放过度问题的可行性方案.光电催化CO_(2)还原可以模拟自然光合作用将CO_(2)还原为多碳产物(C^(2+)).然而,光电催化剂的带隙与太阳辐射光谱不匹配以及载流子的快速复合是限制人工光合作用效率的关键因素.前期研究表明,缺陷工程可有效地增加催化剂活性位点,减小半导体的带隙并增强对光子的捕获能力;而异质结的构筑则可有效提升载流子的分离效率.因此,构建具有较好可见光响应的高效半导体异质结催化剂有望实现催化材料对CO_(2)还原能力和产物选择性的提升.本文通过对金属钛板进行电化学阳极氧化,氨气气氛煅烧得到TiN,然后原位进行部分氧化构筑出结构新颖的TiO_(2)/TiN纳米管异质结材料,再进行配体和钯量子点修饰,得到更加高效的催化电极材料Pd/R-TiO_(2)/TiN,并在三电极系统中研究了其光电催化CO_(2)还原的性能.通过扫描隧道电子显微镜、透射电子显微镜、X射线粉末衍射、X射线光电子能谱、电子顺磁共振谱、X射线吸收近边结构光谱及莫特肖特基曲线等系统考察了催化剂的结构与光电催化性能,证明了n-n同型异质结的成功构筑,且纳米管状的异质结含有丰富的Ti^(3+)和氧空位,具有较好的太阳光捕获能力,保留了间隙金属材料TiN良好的电荷传输能力,提高了光生载流子的分离效率.光电催化CO_(2)还原结果表明,纳米管状Pd/R-TiO_(2)/TiN异质结材料具有较好的CO_(2)还原能力.其中Pd/R-TiO_(2)/TiN-30电极的碳基化合物的产率高达115.9μmolL^(–1)h^(-1)cm^(-2),是纳米颗粒状Pd/R-TiO_(2)/TiN-30异质结材料的2.2倍,且具有最高的C_(2)选择性(65.7%),说明纳米管状结构为反应提供的有限空间有利于C–C耦联.另外,光电联合催化的产率是纯光催化的3.4倍,纯电催化的3.3倍,说明光电协同催化的重要作用.此外,该电极获得的总电池效率(Φ’)为6.0%,远超过植物细胞光合作用的0.4%,并通过^(13)CO_(2)同位素标记实验证实了所有产物均来自CO_(2)气体.原位实况傅里叶变换红外光谱分析结果表明,*COOH和*CHO活性物种是主要中间体.需要强调的是,在氩气饱和(无CO_(2)参与)的条件下检测到了Ti–H振动吸收谱带,且与密度泛函理论计算结果一致.实验和计算相互印证表明,高活性氢原子可以附着在异质结表面与Ti^(3+)位点形成Ti–H物种.此外,计算结果还表明,氮掺杂对催化剂的影响很大,当N物种在晶格中存在可促使晶格氧发生迁移,形成新的氧空位,氧空位有利于CO_(2)分子和中间体的吸附,两者结合从而促进了中间体的生成,加之纳米管的有限空间结构,有助于C–C耦联反应的发生.相反,无N掺杂的纯TiO_(2)锐钛矿不能促进最终Ti–H物种的形成,其反应机理有利于生成CO.结合中间体检测、理论计算结果以及H_(2)^(18)O同位素标记实验,提出了一种氮辅助活性氢反应机理.综上,本研究为理解CO_(2)还原机理提供了有益的参考,也为设计高活性和高C_(2+)选择性的CO_(2)还原光电催化剂提供了思路.

Genetic variation in GmCRP contributes to nodulation in soybean(Glycine max Merr.)

Symbiosis between soybean and rhizobia contributes to soybean yield and quality. Although secreted rhizobial type Ⅲ effectors are known to regulate infection and promote nitrogen fixation, much about them remains unknown. Mutation of NopC, a type Ⅲ effector from Sinorhizobium fredii HH103, reduced nodule numbers and dry weights in 310 soybean accessions, and expression of NopC in soybean hairy roots promoted symbiosis. Based on observed differences in nodule traits between Suinong 14 and Zyd 00,006inoculated with HH103 and the NopC mutant, 11 QTL associated with rhizobia were identified in chromosome segment substitution lines(CSSLs) derived from Suinong 14 and Zyd 00006. Using chromosome fragment insertion, whole-genome sequencing of Suinong 14 and Zyd 00006, and qRT-PCR,Glyma.19G176300(GmCRP) was identified as a candidate gene associated with NopC, and GmCRP was found to be induced by NopC to positively regulate nodulation. SNPs located in the regulatory regions of GmCRP influenced its expression response to NopC, with SNPs contributing to nodulation having been selected during domestication. Our findings reveal the function of a soybean gene encoding a rhizobial type Ⅲ effector that contributes to symbiosis, and will facilitate the practical application of symbiotic nitrogen fixation in molecular breeding.

免疫治疗在局部晚期宫颈癌中的研究进展

宫颈癌是女性最常见的生殖系统恶性肿瘤,高危型人乳头状瘤病毒持续感染是其主要致病因素。传统治疗方法包括手术、放疗和化疗,但对晚期宫颈癌患者的预后并不理想。近年来治疗性疫苗、免疫检查点抑制剂和过继性T细胞疗法等免疫治疗在宫颈癌中应用广泛,显著改善了患者的生存期。本文将对免疫治疗在局部晚期宫颈癌中的临床研究及相关进展进行综述。

反应时间调控的电化学吲哚氧化偶联/偶联环化反应实验设计

吲哚类杂环化合物相关转化是有机化学基础知识。本实验以近年来快速发展的有机电合成技术为手段,以电子为清洁的反应试剂,实现了3-芳基吲哚的脱氢氧化偶联,进而仅延长反应时间即可获得偶联-环化的产物。实验涉及脱氢偶联、串联环化反应等,是重要的有机转化反应。本实验反应现象丰富,所用试剂安全,涉及操作丰富,有助于学生对实验架设、实验观察、实验处理、结果分析的全流程训练。实验经多人多次均可以相近产率重现,产物为固体,可由硅胶柱色谱纯化,结构可通过核磁共振波谱和质谱进行解析,产物具有丰富的荧光性质。该实验立足吲哚转化有机化学基础知识,利用电合成策略,开发含氮杂环化合物的实用合成方法,引导学生注重学习有机化学与物理化学交叉知识,建立学科交叉知识认知,关注学科发展前沿。

Identification of Interlayer in Strong Bottom Water Reservoir and Its Influence on Development Effect

X oilfield is located in Bohai Sea area, in which G oil formation is a typical drape anticline structure, which is composed of multiple sets of thick sandy conglomerate and multiple sets of argillaceous intercalation. From the perspective of development effect, muddy interlayer has a great impact on the oilfield. In this paper, through core identification and well logging identification, the electrical discrimination standard is summarized to identify the interlayer. Through statistics and analysis of the production performance of actual wells, the influence of muddy interlayer on the development performance of oil wells is summarized. This study provides guidance for the development of strong bottom water reservoirs with interlayer.

Numerical Simulation of Residual Oil Displacement in Offshore Strong Bottom Water Reservoir

C oilfield is located in Bohai Bay Basin, a typical strong bottom water reservoir. There is a large amount of remaining oil in the plane and vertical direction, which cannot be used. Therefore, it is urgent to explore the feasibility of changing the development mode of typical sand bodies and displacement mining. In this study, the residual distribution of the bottom water reservoir is studied through numerical simulation. According to the distribution of remaining oil in the middle of the two sand bodies and the local structure of the sand body, it is divided into top remaining oil, edge remaining oil, and inter well-remaining oil. The effects of different angular permeability laws and differential and injected medium on the development effect are simulated and analyzed. It is found that the effect of gas injection is affected by a high mobility ratio, which is easy to break through early, and the effect of gas channeling is limited. Using active water and air-water alternate (ammonia foam) can achieve relatively good results. This experimental study guides tapping the remaining oil potential in the offshore strong bottom water reservoir.

JMJ14 is an H3K4 demethylase regulating flowering time in Arabidopsis

Histone lysine methylation plays an essential role in regulating chromatin functions such as transcription and heterochromatin formation. Histone H3 lysine 4 （H3K4） methylation is linked to active transcription [1, 2]. Recent findings in mammals have demonstrated that histone methylation is reversible by a family of Jumonji C （JmjC） domain-containing proteins. KDM5/ JARID1 family proteins have been shown to be able to demethylate H3K4mel,2,3 in mammals [3]. Previously, we identified six proteins in Arabidopsis showing high sequence similarity to KDM5/JARIDI family proteins [4]. Here we demonstrate that one such protein, JMJ14, is an active histone H3K4 demethylase and is involved in flowering time regulation.

PADI3 induces cell cycle arrest via the Sirt2/AKT/p21 pathway and acts as a tumor suppressor gene in colon cancer

Objective:As a member of the peptidyl arginine deiminase(PAD)family,PADI3 is weakly expressed in colon cancer tissues and highly expressed in adjacent colon cancer tissues.However,the role of PADI3 in colon cancer is unclear.In this study,we investigated the function and molecular mechanism of PADI3 in colon cancer tumorigenesis.Methods:Western blot and real-time PCR were used to detect the expression levels of several genes.CCK-8,flow cytometry(FCM)and colony formation assays were used to examine cell proliferation,the cell cycle and colony formation ability.RNAsequencing analysis was used to study the molecular mechanism of PADI3 in tumorigenesis.A truncation mutation experiment was performed to determine the key functional domain of PADI3.Results:PADI3 overexpression inhibited cell proliferation and colony formation and led to G1 phase arrest in both HCT116(originating from primary colon cancer)and LoVo(originating from metastatic tumor nodules of colon cancer)cells.PADI3-expressing HCT116 cells had a lower tumor formation rate and produced smaller tumors than control cells.PADI3 significantly decreased Sirtuin2(Sirt2)and Snail expression and AKT phosphorylation and increased p21 expression,and Sirt2 overexpression partly reversed the effects induced by PADI3 overexpression.Immunocytochemistry showed that PADI3 is mainly localized in the cytoplasm.Truncation mutation experiments showed that the C-domain is the key domain involved in the antitumor activity of PADI3.Conclusions:PADI3 suppresses Snail expression and AKT phosphorylation and promotes p21 expression by downregulating Sirt2 expression in the cytoplasm,and the C-domain is the key domain for its antitumor activity.

基于微生物灭活芽孢的矿冶粉尘清洁皂的制备及性能研究

矿冶粉尘给一线工人皮肤健康带来严重威胁。通过添加微生物灭活芽孢于清洁皂中,研究了不同芽孢添加量对清洁皂去污能力的影响,对比了硅藻土、活性炭和芽孢添加皂在粉尘去污和重金属去除方面的异同,并通过感官评价测试了三种皂在皮肤清洁方面的效果。结果表明,添加硅藻土可以达到80%的粉尘去除率。而活性炭尽管比表面积高,但微粒细小同样易于吸附于手掌纹理中,造成二次污染,故去除率最低。在基础皂中添加1%(w/w)灭活芽孢对粉尘污染的去污率达到了95%,二次洗涤可将重金属去除率从90%提高到95%以上。感官评价中,黑色活性炭的吸附作用造成了清洗后的手掌依然不够干净,观感最差;硅藻土皂的清洁效果接近日常家用皂,观感居中;而芽孢皂的清洁效果最明显,观感最佳。

Diversity of metabolite accumulation patterns in inner and outer seed coats of pomegranate:exploring their relationship with genetic mechanisms of seed coat development

The expanded outer seed coat and the rigid inner seed coat of pomegranate seeds,both affect the sensory qualities of the fruit and its acceptability to consumers.Pomegranate seeds are also an appealing model for the study of seed coat differentiation and development.We conducted nontarget metabolic profiling to detect metabolites that contribute to the morphological differentiation of the seed coats along with transcriptomic profiling to unravel the genetic mechanisms underlying this process.Comparisons of metabolites in the lignin biosynthetic pathway accumulating in seed coat layers at different developmental stages revealed that monolignols,including coniferyl alcohol and sinapyl alcohol,greatly accumulated in inner seed coats and monolignol glucosides greatly accumulated in outer seed coats.Strong expression of genes involved in monolignol biosynthesis and transport might explain the spatial patterns of biosynthesis and accumulation of these metabolites.Hemicellulose constituents and flavonoids in particular accumulated in the inner seed coat,and candidate genes that might be involved in their accumulation were also identified.Genes encoding transcription factors regulating monolignol,cellulose,and hemicellulose metabolism were chosen by coexpression analysis.These results provide insights into metabolic factors influencing seed coat differentiation and a reference for studying seed coat developmental biology and pomegranate genetic improvement.

Mycorrhiza improves plant growth and photosynthetic characteristics of tea plants in response to drought stress

Tea plants are sensitive to soil moisture deficit,with the level of soil water being a critical factor affecting their growth and quality.Arbuscular mycorrhizal fungi(AMF)can improve water and nutrient absorption,but it is not clear whether AMF can improve the photosynthetic characteristics of tea plants.A potted study was conducted to determine the effects of Claroideoglomus etunicatum on plant growth,leaf water status,pigment content,gas exchange,and chlorophyll fluorescence parameters in Camellia sinensis cv.Fuding Dabaicha under well-watered(WW)and drought stress(DS)conditions.Root mycorrhizal colonization and soil hyphal length were significantly reduced by the eightweek DS treatment.AMF inoculation displayed a significant increase in shoot and root biomass production.The relative water content,leaf water potential,nitrogen balance index,pigment content,maximum photometric effect(Fv/Fm,QY_max),and steady-state photometric effect Y(II)(QY_Lss)decreased dramatically,while the leaf water saturation deficit and steady-state non-photochemical fluorescence quenching(NPQ_Lss)generally increased under DS conditions.Mycorrhizal treatment induced significantly higher relative water content,leaf water potential,nitrogen balance index,pigment(chlorophyll,flavonoid,and anthocyanin)content,net photosynthesis rate,transpiration rate,stomatal conductance,intercellular CO_(2)concentration,QY_max,and QY_Lss;however,it resulted in a lower leaf water saturation deficit and NPQ_Lss under both WW and DS conditions,as compared with nonmycorrhizal plants.These results imply that AMF promoted tea plant growth and alleviated negative effects of DS by promoting gas exchange,regulating the water status of leaves,and regulating photosynthetic parameters.

Revisiting the Concentration Observations and Source Apportionment of Atmospheric Ammonia

While China’s Air Pollution Prevention and Control Action Plan on particulate matter since 2013 has reduced sulfate significantly,aerosol ammonium nitrate remains high in East China.As the high nitrate abundances are strongly linked with ammonia,reducing ammonia emissions is becoming increasingly important to improve the air quality of China.Although satellite data provide evidence of substantial increases in atmospheric ammonia concentrations over major agricultural regions,long-term surface observation of ammonia concentrations are sparse.In addition,there is still no consensus on whether agricultural or non-agricultural emissions dominate the urban ammonia budget.Identifying the ammonia source by nitrogen isotope helps in designing a mitigation strategy for policymakers,but existing methods have not been well validated.Revisiting the concentration measurements and identifying source apportionment of atmospheric ammonia is thus an essential step towards reducing ammonia emissions.

GmGPDH12,a mitochondrial FAD-GPDH from soybean, increases salt and osmotic stress resistance by modulating redox state and respiration

In plants,glycerol-3-phosphate dehydrogenase(GPDH)catalyzes the interconversion of glycerol-3-phosphate(G3P)and dihydroxyacetone phosphate(DHAP)coupled to the reduction/oxidation of the nicotinamide adenine dinucleotide(NADH)pool,and plays a central role in glycerolipid metabolism and stress response.Previous studies have focused mainly on the NAD+-dependent GPDH isoforms,neglecting the role of flavin adenine dinucleotide(FAD)-dependent GPDHs.We isolated and characterized three mitochondrialtargeted FAD-GPDHs in soybean,of which one isoform(GmGPDH12)showed a significant transcriptional response to NaCl and mannitol treatments,suggesting the existence of a major FAD-GPDH isoform acting in soybean responses to salt and osmotic stress.An enzyme kinetic assay showed that the purified GmGPDH12 protein possessed the capacity to oxidize G3P to DHAP in the presence of FAD.Overexpression and RNA interference of GmGPDH12 in soybean hairy roots resulted in elevated tolerance and sensitivity to salt and osmotic stress,respectively.G3P contents were significantly lower in GmGPDH12-overexpressing hair roots and higher in knockdown hair roots,indicating that GmGPDH12 was essential for G3P catabolism.A significant perturbation in redox status of NADH,ascorbic acid(ASA)and glutathione(GSH)pools was observed in GmGPDH12-knockdown plants under stress conditions.The impaired redox balance was manifested by higher reactive oxygen species generation and consequent cell damage or death;however,overexpressing plants showed the opposite results for these traits.GmGPDH12 overexpression contributed to maintaining constant respiration rates under salt or osmotic stress by regulating mRNA levels of key mitochondrial respiratory enzymes.This study provides new evidence for the roles of mitochondria-localized GmGPDH12 in conferring resistance to salt or osmotic stress by maintaining cellular redox homeostasis,protecting cells and respiration from oxidative injury.

Production of reactive oxygen species by PuRBOHF is critical for stone cell development in pear fruit

The production of reactive oxygen species(ROS)by NADPH oxidase,which is also referred to as respiratory burst oxidase homolog(RBOH),affects several processes in plants.However,the role of RBOHs in cell wall lignification is not well understood.In this study,we show that PuRBOHF,an RBOH isoform,plays an important role in secondary wall formation in pear stone cells.ROS were closely associated with lignin deposition and stone cell formation according to microscopy data.In addition,according to the results of an in situ hybridization analysis,the stage-specific expression of PuRBOHF was higher in stone cells than in cells of other flesh tissues.Inhibitors of RBOH activity suppressed ROS accumulation and stone cell lignification in pear fruit.Moreover,transient overexpression of PuRBOHF caused significant changes in the amount of ROS and lignin that accumulated in pear fruit and flesh calli.We further showed that PuMYB169 regulates PuRBOHF expression,while PuRBOHF-derived ROS induces the transcription of PuPOD2 and PuLAC2.The findings of this study indicate that PuRBOHF-mediated ROS production,which is regulated by a lignin-related transcriptional network,is essential for monolignol polymerization and stone cell formation in pear fruit.

SOME METRIC AND TOPOLOGICAL PROPERTIES OF NEARLY STRONGLY AND NEARLY VERY CONVEX SPACES

We obtain characterizations of nearly strong convexity and nearly very convexity by using the dual concept of S and WS points,related to the so-called Rolewicz’s property(α).We give a characterization of those points in terms of continuity properties of the identity mapping.The connection between these two geometric properties is established,and finally an application to approximative compactness is given.

A soybean NAC homolog contributes to resistance to Phytophthora sojae mediated by dirigent proteins

Phytophthora sojae infection severely impairs soybean production. We previously identified a dirigent protein, Gm DRR1(Glycine max Disease Resistant Response 1), that increases soybean resistance to P.sojae. However, the molecular basis of Gm DRR1 function remained largely uncharacterized. In the present study, analysis of Gm DRR1-RNAi, Gm DRR1-overexpressing, and CRISPR/Cas9-derived Gmdrr1 mutant lines revealed that Gm DRR1 expression significantly restricted P. sojae growth. Combining coimmunoprecipitation with liquid chromatography–tandem mass spectrometry revealed a Gm DRR1-interacting protein, Gm DRR2, which is homologous to Gm DRR1. An E-coniferyl alcohol coupling assay indicated that Gm DRR1 promotes the synthesis of(+)-pinoresinol, which helps to protect plants from P. sojae. The Gm NAC1(Glyma.05 G025500) transcription factor bound to the Gm DRR1 promoter both in vitro and in vivo to upregulate Gm DRR1 expression. Soybean resistance to P. sojae was increased by overexpression of Gm NAC1. Our findings suggest a novel signaling pathway involving a NAC transcription factor that mediates soybean resistance to P. sojae. Specifically, Gm NAC1 directly induces Gm DRR1 expression to increase resistance of soybean plants to P. sojae.

The Forgotten Nutrient——The Role of Nitrogen in Permafrost Soils of Northern China

1.Introduction:permafrost carbon and nitrogen feedback to climate change Permafrost refers to any ground,including soils,sediments and rocks,with a temperature at or below the freezing point of water(0℃)for two or more consecutive years(Biskaborn et al.,2019).

Effect of activation of PPARγ on cell cycle progression in human gastric carcinoma cells

Objective： To investigate the effect of activation of peroxisome proliferator-activated receptor gamma （PPARy） on cell cycle arrest of gastric carcinoma cell line MGC803. Methods： The inhibitory of pioglitazone （PGZ） on proliferation of MGC803 cells was analyzed by MTT assay. Cell cycle was detected by flow cytometry （FCM）. The expressions of PPARy, cyclin D1 and cell cycle protein-dependent kinase CDK4 in MGC803 cells were detected by reverse transcriptase-polymerase chain reaction （RT-PCR）. Results： Treatment with 0.1-10 μmol/L PGZ for 96 h significantly inhibited cell proliferation. The proportion of MGC803 cells at G1 phase was significantly increased when treated with 10 μmol/L PGZ for 48, 72 and 96 h, and showed an apparent G1 phase arrest. The expression of PPARy was at a low level in MGC803 cells and significantly up-regulated when treated with 10 μmol/L PGZ for 48 h （P〈0.01）. The expression of CDK4 in MGC803 cells was remarkably down-regulated when treated with 10 μmol/L PGZ for 48 h and the expression of cyclin D1 was slightly down-regulated （P 〈 0.01）. Conclusion： Activation of PPARy significantly induced G1 phase arrest, which was associated with down-regulation of the expressions of CDK4 and cyclin DI.

Primary Report on Influences of the Meteorological Factors on High-quality Summer Millet Production in North Henan Province

[ Objective] The research aimed to study influences of meteorological factors on high-quality summer millet production in north Henan Province. [ Method] By using summer millet yield and corresponding meteorological data in Anyang of Henan, influence of rainfall on yield of new high-quality summer millet vadety during 2003 -2012 was analyzed. [Result]Rainfall during May -July had the maximum influence on summer millet yield. Correlation regression equation was Y = 0.738 8X ＋ 159.991 （ R = 0.982 04）, reaching extremely significant level. [ Conclusion ] The re- search could provide scientific basis for sufficiently using local meteorological condition, breeding high-quality summer millet variety and its high yield.