Equilibrium constants for the binding of azide to ferri-cytochrome c at temperature range of 305—325 K were determined at pH=7 by using ~1H double resonance method.Thermodynamic values(⊿H^o=-34.5 kJ/mol,⊿S^o=-100 J...Equilibrium constants for the binding of azide to ferri-cytochrome c at temperature range of 305—325 K were determined at pH=7 by using ~1H double resonance method.Thermodynamic values(⊿H^o=-34.5 kJ/mol,⊿S^o=-100 J/mol)were obtained from van't Hoff's relation and were compared with those for azide binding to other ferric hemeproteins.The reason of lower affinity of cytochrome c for azide was discussed.展开更多
文摘Equilibrium constants for the binding of azide to ferri-cytochrome c at temperature range of 305—325 K were determined at pH=7 by using ~1H double resonance method.Thermodynamic values(⊿H^o=-34.5 kJ/mol,⊿S^o=-100 J/mol)were obtained from van't Hoff's relation and were compared with those for azide binding to other ferric hemeproteins.The reason of lower affinity of cytochrome c for azide was discussed.
