Effect of naphthalene acetic acid on proliferation and apoptosis of mice hepatocytes

Objective To investigate the effect of naphthalene acetic acid(NAA) on proliferation and apoptosis of mice hepatocytes.Methods Fifty mice were randomly divided into five groups: positive control,normal control,low,middle and high dose of NAA groups.Mice in low,middle and high dose of naphthalene acetic acid groups were fed with 200 mg/kg,1 000 mg/kg and 5 000 mg/kg NAA animal food,respectively.Mice in positive control,normal control groups were fed with usual animal food.After 4 weeks,mice were sacrificed and liver was separated.Three days before scarification,mice in positive control group were given 20 mg/kg ascyclophosphami daily by intraperitoneal injection.The proliferative activity of hepatocytes was detected by MTT assay.The expressions of PCNA protein and Caspase-3 were measured by immunohistochemistry method.Results The proliferative activities of mice hepatocytes in high dose group and normal control group were 0.794±0.019 and 1.055±0.019, respectively.The expressions of PCNA protein and Caspase-3 in high dose group were 11.2% and 37.9%,and those in the normal group were 33.1% and 6.3%, respectively.All differences were significant(P<0.01).Conclusion High dose of NAA acid could significantly inhibit proliferation and increase apoptosis of hepatocytes in mice.