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Transcriptomics integrated with metabolomics reveals the mechanism of CaCl_(2)-HCl electrolyzed water-induced glucosinolate biosynthesis in broccoli sprouts
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作者 cui li Shuhui Song +1 位作者 Yanan He Haijie liu 《Food Science and Human Wellness》 SCIE CSCD 2024年第2期801-812,共12页
Glucosinolates are important phytochemicals in Brassicaceae.We investigated the effect of CaCl_(2)-HCl electrolyzed water(CHEW)on glucosinolates biosynthesis in broccoli sprouts.The results showed that CHEW treatment ... Glucosinolates are important phytochemicals in Brassicaceae.We investigated the effect of CaCl_(2)-HCl electrolyzed water(CHEW)on glucosinolates biosynthesis in broccoli sprouts.The results showed that CHEW treatment significantly decreased reactive oxygen species(ROS)and malondialdeh yde(MDA)contents in broccoli sprouts.On the the 8^(th)day,compared to tap water treatment,the the total glucosinolate content of broccoli sprouts with CHEW treatment increased by 10.6%and calcium content was dramatically enhanced from 14.4 mg/g DW to 22.7 mg/g DW.Comparative transcriptome and metabolome analyses revealed that CHEW treatment activated ROS and calcium signaling transduction pathways in broccoli sprouts and they interacted through MAPK cascades.Besides,CHEW treatment not only promoted the biosynthesis of amino acids,but also enhanced the expression of structural genes in glucosinolate synthesis through transcription factors(MYBs,bHLHs,WRKYs,etc.).The results of this study provided new insights into the regulatory network of glucosinolates biosynthesis in broccoli sprouts under CHEW treatment. 展开更多
关键词 Broccoli sprouts CaCl_(2)-HCl electrolyzed water GLUCOSINOLATES TRANSCRIPTOMICS Metabolomics
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LiOH/MgO新型固体碱的制备及催化合成米糠油甲酯
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作者 王嘉诚 李盼 +2 位作者 黄巍 崔励 张江华 《大连工业大学学报》 CAS 2024年第5期359-363,共5页
以浸渍法制备了LiOH/MgO固体碱催化剂,并用于催化合成米糠油甲酯,研究了LiOH/MgO催化剂的制备工艺,采用X射线衍射法、热重分析法等对催化剂进行表征,采用气相色谱外标法分析米糠油甲酯成分及质量分数。通过响应面实验优化米糠油甲酯的... 以浸渍法制备了LiOH/MgO固体碱催化剂,并用于催化合成米糠油甲酯,研究了LiOH/MgO催化剂的制备工艺,采用X射线衍射法、热重分析法等对催化剂进行表征,采用气相色谱外标法分析米糠油甲酯成分及质量分数。通过响应面实验优化米糠油甲酯的合成工艺:当LiOH负载率为载体质量的23.8%、醇油摩尔比为14.3∶1、催化剂用量为米糠油质量的7.5%、反应时间为4h、反应温度为100℃时,收率可达87.90%。结果表明,LiOH/MgO固体碱催化剂对米糠油甲酯化反应具有较好的催化活性,有利于米糠油这一农副产物得到充分利用。 展开更多
关键词 米糠油 米糠油甲酯 固体碱 响应面法
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血液透析患者移植物动静脉内瘘并发症预防与管理的证据总结 被引量:2
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作者 张彬彬 魏丽丽 +3 位作者 崔莉 郭菁 郝小磊 徐向朋 《中国护理管理》 CSCD 北大核心 2024年第1期95-101,共7页
目的 :检索、评价及总结维持性血液透析患者移植物动静脉内瘘并发症预防与管理的相关证据,为临床减少血液透析患者移植物动静脉内瘘相关并发症的发生提供循证依据。方法 :系统检索UpToDate、BMJ最佳临床实践、澳大利亚乔安娜布里格斯研... 目的 :检索、评价及总结维持性血液透析患者移植物动静脉内瘘并发症预防与管理的相关证据,为临床减少血液透析患者移植物动静脉内瘘相关并发症的发生提供循证依据。方法 :系统检索UpToDate、BMJ最佳临床实践、澳大利亚乔安娜布里格斯研究所(JBI)循证卫生保健中心、美国指南网、苏格兰学院间指南网站、PubMed、中国知网、中华医学期刊全文数据库、医脉通、万方数据库、维普数据库等与血液透析患者移植物动静脉内瘘并发症预防与管理相关的文献,包括临床决策、证据总结、指南、专家共识、系统评价、随机对照试验等,检索时限为建库至2023年6月30日。由专业循证护理人员对文献进行质量评价及证据提取、汇总。结果 :共纳入15篇文献,包括临床决策7篇、指南5篇、专家共识1篇、队列研究1篇、随机对照试验1篇,结合临床专业人员意见汇总了5个主题,包括围手术期管理、监测与筛查、使用与维护、人员与培训及健康教育,共33条证据。结论 :该研究总结的维持性血液透析患者移植物动静脉内瘘并发症预防与管理的证据具有全面性和科学性,为临床医护人员规范移植物动静脉内瘘的管理提供循证依据。 展开更多
关键词 血透透析 移植物动静脉内瘘 并发症 证据总结 循证护理学
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Arbuscular mycorrhizal fungi combined with exogenous calcium improves the growth of peanut(Arachis hypogaea L.)seedlings under continuous cropping 被引量:10
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作者 cui li GUO Feng +6 位作者 ZHANG Jia-lei YANG Sha MENG Jing-jing GENG Yun WANG Quan li Xin-guo WAN Shu-bo 《Journal of Integrative Agriculture》 SCIE CAS CSCD 2019年第2期407-416,共10页
The growth and yield of peanut are negatively affected by continuous cropping.Arbuscular mycorrhizal fungi(AMF)and calcium ions(Ca^(2+))have been used to improve stress resistance in other plants,but little is known a... The growth and yield of peanut are negatively affected by continuous cropping.Arbuscular mycorrhizal fungi(AMF)and calcium ions(Ca^(2+))have been used to improve stress resistance in other plants,but little is known about their roles in peanut seedling growth under continuous cropping.This study investigated the possible roles of the AMF Glomus mosseae combined with exogenous Ca^(2+)in improving the physiological responses of peanut seedlings under continuous cropping.G.mosseae combined with exogenous Ca^(2+)can enhance plant biomass,Ca^(2+)level,and total chlorophyll content.Under exogenous Ca^(2+)application,the F_v/F_m in arbuscular mycorrhizal(AM)plant leaves was higher than that in the control plants when they were exposed to high irradiance levels.The peroxidase,superoxide dismutase,and catalase activities in AM plant leaves also reached their maximums,and accordingly,the malondialdehyde content was the lowest compared to other treatments.Additionally,root activity,and content of total phenolics and flavonoids were significantly increased in AM plant roots treated by Ca^(2+)compared to either G.mosseae inoculation or Ca^(2+)treatment alone.Transcription levels of AhCaM,AhCDPK,AhRAM1,and AhRAM2 were significantly improved in AM plant roots under exogenous Ca^(2+)treatment.This implied that exogenous Ca^(2+)might be involved in the regulation of G.mosseae colonization of peanut plants,and in turn,AM symbiosis might activate the Ca^(2+)signal transduction pathway.The combination of AMF and Ca^(2+)benefitted plant growth and development under continuous cropping,suggesting that it is a promising method to cope with the stress caused by continuous cropping. 展开更多
关键词 ARACHIS HYPOGAEA L. ARBUSCULAR MYCORRHIZAL fungi continuous CROPPING exogenous calcium
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Inhibition of LOX-1 alleviates the proinflammatory effects of high-mobility group box 1 in Aspergillus fumigatus keratitis 被引量:9
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作者 Jia-Qian Jiang cui li +7 位作者 Cong-Xian cui Yu-Na Ma Gui-Qiu Zhao Xu-Dong Peng Qiang Xu Qian Wang Guo-Qiang Zhu Chen-Yu li 《International Journal of Ophthalmology(English edition)》 SCIE CAS 2019年第6期898-903,共6页
AIM: To investigate the inflammatory amplification effect of high-mobility group box 1(HMGB1) in Aspergillus fumigatus(A. fumigatus) keratitis and the relationship between lectin-like oxidized low-density lipoprotein ... AIM: To investigate the inflammatory amplification effect of high-mobility group box 1(HMGB1) in Aspergillus fumigatus(A. fumigatus) keratitis and the relationship between lectin-like oxidized low-density lipoprotein receptor 1(LOX-1) and HMGB1 in keratitis immune responses.METHODS: Phosphate buffer saline(PBS), and Boxb were injected into BALB/c mice subconjunctivally before the corneas were infected with A. fumigatus. RAW264.7 macrophages and neutrophils were pretreated with PBS and Boxb to determine the HMGB1 inflammatory amplification effects. Abdominal cavity extracted macrophages were pretreated with Boxb and Poly(I)(a LOX-1 inhibitor) before A. fumigatus hyphae stimulation to prove the the relationship between the two molecules. LOX-1, interleukin-1β(IL-1β), tumor necrosis factor-α(TNF-α), macrophage inflammatory protein-2(MIP-2) and IL-10 were assessed by polymerase chain reaction and Western blot.RESULTS: Pretreatment with Boxb exacerbated corneal inflammation. In macrophages and neutrophils, A. fumigatus induced LOX-1, IL-1β, TNF-α and MIP-2 expression in Boxb group was higher than those in PBS group. Poly(I) treatments before infection alleviated the proinflammatory effects of Boxb in abdominal cavity extracted macrophages. Pretreatment with Boxb did not influence Dectin-1 mRNA levels in macrophages and neutrophils.CONCLUSION: In fungal keratitis, HMGB1 is a proinflammatory factor in the first line of immune response. HMGB1 mainly stimulates neutrophils and macrophages to produce inflammatory cytokines and chemokines during the immune response. LOX-1 participates in HMGB1 induced inflammatory exacerbation in A. fumigatus keratitis. 展开更多
关键词 Aspergillus FUMIGATUS KERATITIS HIGH-MOBILITY GROUP BOX 1 LOX-1
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Production of interleukin-1β related to mammalian target of rapamycin/Toll-like receptor 4 signaling pathway during Aspergillus fumigatus infection of the mouse cornea 被引量:6
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作者 Rui Xu Jing lin +4 位作者 Gui-Qiu Zhao cui li Cheng-Ye Che Qiang Xu Min liu 《International Journal of Ophthalmology(English edition)》 SCIE CAS 2018年第5期712-718,共7页
AIM:To elucidate the effect of rapamycin on regulating the production of interleukin(IL)-1β in Aspergillus fumigatus(A.fumigatus)-induced keratitis and to verify whether the expression of IL-1β in A.fumigatus k... AIM:To elucidate the effect of rapamycin on regulating the production of interleukin(IL)-1β in Aspergillus fumigatus(A.fumigatus)-induced keratitis and to verify whether the expression of IL-1β in A.fumigatus keratitis is associated with the mammalian target of rapamycin(mT OR)/Toll-like receptor 4(TLR4) signaling pathway.METHODS:Fungal keratitis mouse models of susceptible C57 BL/6 mice were established using A.fumigatus.The mice were subsequently treated with rapamycin.The protein levels of p-mT OR,TLR4,and IL-1β in normal and infected corneal tissue were measured by Western blot.The TLR4 and IL-1β m RNA levels were determined by real-time polymerase chain reaction(PCR).RESULTS:In C57 BL/6 mice,rapamycin treatment decreased the clinical scores and production of the pro-inflammatory cytokine,IL-1β.The expression of TLR4,stimulated by A.fumigatus,was reduced as well when the mT OR signaling pathway was suppressed by rapamycin.CONCLUSION:Rapamycin is beneficial for the outcome of fungal keratitis and has an inhibitory effect expression of the inflammatory cytokine IL-1β.The inhibitory effect on IL-1β expression can be associated with the mT OR/TLR4 signaling pathway in A.fumigatus infection in mice. 展开更多
关键词 KERATITIS INTERLEUKIN-1Β mammalian target of rapamycin Toll-like receptor 4 mice
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Expression of dectin-1 during fungus infection in human corneal epithelial cells 被引量:6
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作者 cui li Gui-Qiu Zhao +6 位作者 Cheng-Ye Che Na li Jing lin Qiang Xu Qian Wang Ying liu Sheng Qiu 《International Journal of Ophthalmology(English edition)》 SCIE CAS 2014年第1期34-37,共4页
AIM: To evaluate the expression of dendritic cell-associated C-type lectin-1(dectin-1) in human corneal epithelial(HCE) cells infected by fungus. · METHODS: A total of 20 cases of healthy donor corneas were group... AIM: To evaluate the expression of dendritic cell-associated C-type lectin-1(dectin-1) in human corneal epithelial(HCE) cells infected by fungus. · METHODS: A total of 20 cases of healthy donor corneas were group A,and 20 patients(20 eyes) suffered from fungal keratitis(FK) composed group B. Real-time qPCR and immunohistochemistry were applied to detect dectin-1 expression in corneal epithelium of both groups. HCE cells were cultured with aspergillus fumigatus(AF) antigens in vitro. The expression of dectin-1 mRNA was measured by real-time qPCR at the stimulation of 0,4,8 and 24h separately. Dectin-1 protein was detected by immunocytochemistry at 0 and 24h separately. ·RESULTS: Dectin-1 expressed in corneal epithelium of normal persons and FK patients. Vitro cellular experiment showed that the expression of dectin-1 mRNA in HCE cells began to increase after stimulation of AF antigens at 4h,and dectin-1 protein expression increased after stimulation at 24h. · CONCLUSION: Dectin-1 expressed in corneal epithelium of normal persons. AF antigens stimulation can elevate the expression of dectin-1 in HCE cells in vitro. 展开更多
关键词 DECTIN-1 corneal epithelial cells fungal keratitis HUMAN
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Role of the IL-33/ST2/p38 signaling pathway in the immune response of corneal epithelial cells to Aspergillus fumigatus infection 被引量:4
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作者 Jia You Jing lin +6 位作者 Yi-Fan Zhou Xu-Dong Peng Hong He cui li Guo-Qiang Zhu Xue-Qi Zhao Gui-Qiu Zhao 《International Journal of Ophthalmology(English edition)》 SCIE CAS 2019年第4期549-556,共8页
AIM: To investigate the expression of interleukin(IL)-33 in the cornea and human corneal epithelial cells(HCECs) exposed to Aspergillus fumigatus(A. fumigatus), and to determine the function of IL-33/ST2/p38 signaling... AIM: To investigate the expression of interleukin(IL)-33 in the cornea and human corneal epithelial cells(HCECs) exposed to Aspergillus fumigatus(A. fumigatus), and to determine the function of IL-33/ST2/p38 signaling pathway in the immune response of corneal epithelial cells to A. fumigatus infection.METHODS: The mRNA and protein expression of IL-33 in HCECs and mice corneas were examined by quantitative real-time reverse transcription polymerase chain reaction(q RT-PCR) and Western blot analysis, respectively. IL-33 expression was also detected in cornea samples from healthy donors and patients with fungal keratitis with immunohistochemistry. The cultured HCECs were treated with inactive A. fumigatus hyphae at various concentrations with or without recombinant human IL-33 protein, soluble recombinant ST2 protein, specific ST2 neutralizing antibody, or the mitogen-activated protein kinase(MAPK) p38 inhibitor SB203580 for evaluation of the expression and activation of IL-33/ST2/p38 signaling in the regulation of proinflammatory cytokines. The production levels of IL-6 and IL-1β were determined by qR T-PCR and enzymelinked immunosorbent assay(ELISA). The proliferation of HCECs was determined by a Cell Counting Kit-8(CCK8) assay and cell count.RESULTS: IL-33 expression levels increased in the corneal tissues of patients with fungal keratitis and in mice corneas of experimental A. fumigatus infection,as well as in HCECs with infection of A. fumigatus. A. fumigatus strongly stimulated HCECs-generated proinflammatory cytokine(IL-6 and IL-1β) production at both the mRNA and protein levels. This production of proinflammatory mediators stimulated by A. fumigatus was further stimulated by IL-33 and was prevented by soluble ST2 protein or ST2 neutralizing antibody. Moreover, IL-33 naturally promoted the p38 phosphorylation induced by A. fumigatus, which was suppressed by soluble ST2 protein. The MAPK p38 inhibitor SB203580 also inhibited the A. fumigatus-induced proinflammatory cytokine production. IL-33 administration for 48 h and 72 h promoted the proliferation of HCECs, which was attenuated by treatment with soluble recombinant human ST2 protein.CONCLUSION: A. fumigatus elevates IL-33 expression in human and mice corneas and HCECs. Thus, IL-33/ST2/p38 signaling may play an important role in amplifying the immune response of corneal epithelial cells to A. fumigatus infection. Besides, IL-33 promotes the cell proliferation of HCECs via its receptor ST2. These findings suggest a novel autocrine mechanism of amplification of the fungalinduced inflammatory response in the corneal epithelium, highlighting a potential therapeutic target for fungal keratitis. 展开更多
关键词 ST2 INTERLEUKIN 33 Aspergillus FUMIGATUS CORNEA P38 MITOGEN-ACTIVATED protein kinase proliferation
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Effects of different drying methods on quality,bacterial viability and storage stability of probiotic enriched apple snacks 被引量:5
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作者 cui li NIU li-ying +4 位作者 li Da-jing liU Chun-quan liU Ying-ping liU Chun-ju SONG Jiang-feng 《Journal of Integrative Agriculture》 SCIE CAS CSCD 2018年第1期247-255,共9页
Effects of four different drying methods on the colour, texture, sensory quality, microstructure, bacterial viability and storage stability of probiotic-enriched apple snacks were assessed. The drying methods were air... Effects of four different drying methods on the colour, texture, sensory quality, microstructure, bacterial viability and storage stability of probiotic-enriched apple snacks were assessed. The drying methods were air drying (AD), freeze drying (FD), freeze drying followed by microwave vacuum drying (FD+MVD) and air drying followed by explosion puffing drying (AD+EPD). Overall, FD+MVD can be used as a suitable drying method for the development of probiotic enriched apple snacks in consideration of colour, texture, sensory quality, bacterial viability and storage stability. Probiotic bacteria in FD+MVD-dried samples remained above 1×106 CFU g 1 for 120 days at 25℃C. Interestingly, bacterial viability in FD+MVD-dried samples turned out to be significantly higher than FD-dried samples during storage for 120 days. 展开更多
关键词 DRYING apple snacks probiotic viability microwave vacuum
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Sulforaphane attenuates dextran sodium sulphate induced intestinal inflammation via IL-10/STAT3 signaling mediated macrophage phenotype switching 被引量:6
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作者 Yuyang Sun Jiqing Tang +2 位作者 cui li Jun liu Haijie liu 《Food Science and Human Wellness》 SCIE 2022年第1期129-142,共14页
Innate immunity,particularly macrophages,is critical for intestinal homeostasis.Sulforaphane,a dietary isothiocyanate from cruciferous vegetables,has been reported to protect against intestinal inflammation.However,th... Innate immunity,particularly macrophages,is critical for intestinal homeostasis.Sulforaphane,a dietary isothiocyanate from cruciferous vegetables,has been reported to protect against intestinal inflammation.However,the role of macrophages in sulforaphane mediated intestinal inflammation and the underlying molecular mechanisms have not been studied yet.In this study,sulforaphane effectively attenuated dextran sodium sulphate(DSS)induced intestinal inflammation in murine model.Of note,sulforaphane skewed the switching from classically(M1)to alternatively(M2)activated phenotype both in intestinal and bone marrow-derived macrophages(BMDMs).The expression levels of M1 associated maker genes induced by DSS or lipopolysaccharide(LPS)plus interferon gamma-γ(IFN-γ)were suppressed by sulforaphane while M2 marker gene expression levels were improved.This resulted in alteration of inflammatory mediators,particularly interleukin-10(IL-10),both in colon tissues and culture medium of BMDMs.Subsequently,IL-10 was found to mediate the sulforaphane induced M2 phenotype switching of BMDMs through the activation of STAT3 signaling.This was confirmed by immunofluorescence analysis with increased number of p-STAT3-positive cells in the colon sections.Moreover,anti-IL-10 neutralizing antibody significantly interfered M2 phenotyping of BMDMs induced by sulforaphane with reduced STAT3 phosphorylation.Findings here introduced a potential utilization of sulforaphane for intestinal inflammation treatment with macrophages as the therapeutic targets. 展开更多
关键词 BMDMs Intestinal inflammation IL-10 Macrophage phenotype STAT3 SULFORAPHANE
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Dectin-1 expression at early period of Aspergillus fumigatus infection in rat's corneal epithelium 被引量:4
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作者 Cheng-Ye Che cui li +5 位作者 Ang Gao Jing lin li-li Zhang Qiang Xu Qian Wang Gui-Qiu Zhao 《International Journal of Ophthalmology(English edition)》 SCIE CAS 2013年第1期30-33,共4页
AIM:To investigate the expression of dendritic cell-associated C-type lectin-1 (dectin-1) at the early period of Aspergillus fumigatus infection in rat’s corneal epithelium. ·METHODS:A total of 72 Wistar rats we... AIM:To investigate the expression of dendritic cell-associated C-type lectin-1 (dectin-1) at the early period of Aspergillus fumigatus infection in rat’s corneal epithelium. ·METHODS:A total of 72 Wistar rats were randomly divided into three groups:A, B and C. The right eyes were chosen as experimental eyes. Group A was control group. Rats in group B were not inoculated with Aspergillus fumigatus. Group C was taken as Aspergillus fumigatus keratitis model. Rats in group B and C (six from each group) were executed randomly at 4, 8, 16 and 24 hours after experimental model being established to assess the expression of dectin-1 mRNA through real-time PCR. Another six rats in group B and C were executed randomly at 24 hours to assess the expression of dectin-1 protein through immunohistochemistry. ·RESULTS:The results of real-time PCR indicated that dectin-1 mRNA expression was low in corneal epithelium of normal rats’. There was no significantly difference of dectin-1 mRNA expression in group A and B (P 】0.05). The expression of Aspergillus fumigatus infected corneal epithelium increased gradually after 8 hours in group C. The synchronous expression of group A and C had significant difference (P 【0.01). Immunohistochemisty discovered that dectin-1 receptor existed in normal rat’s corneal epithelium . Dectin-1 protein increased after 24 hours in group C. There was a significant difference of synchronous expression in group B and C(P【0.01). · CONCLUSION:Dectin-1 exists in rat’s cornealepithelium and its expression significantly increases at the early period of Aspergillus fumigatus infection. Dectin-1 is a pattern recognition receptor that expresses in corneal epithelium and involves in immune response to Aspergillus fungal keratitis. 展开更多
关键词 KERATITIS Aspergillus fumigatus DECTIN-1 RAT
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Effect of Processing on Taste Quality and Health-Relevant Functionality of Sweet Potato Tips 被引量:2
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作者 cui li liU Chun-quan +1 位作者 li Da-jing SONG Jiang-feng 《Agricultural Sciences in China》 CAS CSCD 2011年第3期456-462,共7页
The effects of fermenting, boiling, and steaming processes on the levels of free amino acids, organic acids, antioxidant properties (FRAP and DPPH), phenolic compounds, and angiotensine converting enzyme (ACE) inh... The effects of fermenting, boiling, and steaming processes on the levels of free amino acids, organic acids, antioxidant properties (FRAP and DPPH), phenolic compounds, and angiotensine converting enzyme (ACE) inhibition of sweet potato tips were investigated. Seventeen free amino acids were found in the tips. Fermenting, boiling, and steaming processes significantly reduced total free amino acid contents and steaming processes caused smaller losses than fermenting and boiling processes. Five organic acids were investigated in the tips. In comparison with the total organic acid content of unprocessed sweet potato tips, steaming and boiling processes significantly reduced it, while fermenting process increased it. Chlorogenic acid, rutin, and quercetin were investigated in the tips. The total phenolic compounds contents of sweet potato tips significantly increased by steaming and boiling processes, but a substantial loss was caused by fermenting process. A similar result was shown in FRAP value. The result of ACE inhibitory activity indicated that boiling, steaming, and fermenting processes significantly increased ACE inhibitory activity. 展开更多
关键词 sweet potato tips amino acids phenolic compounds organic acids ACE antioxidant capacities
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High-mobility group box1 as an amplifier of immune response and target for treatment in Aspergillus fumigatus keratitis 被引量:2
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作者 Meng-Qi Wu cui li +7 位作者 li-Na Zhang Jing lin Kun He Ya-Wen Niu Cheng-Ye Che Nan Jiang Jia-Qian Jiang Gui-Qiu Zhao 《International Journal of Ophthalmology(English edition)》 SCIE CAS 2020年第5期708-717,共10页
AIM:To determine the roles of high-mobility group box1(HMGB1)in pro-inflammation,host immune response and its potential target for treatment in Aspergillus fumigatus(A.fumigatus)keratitis.METHODS:Expression of HMGB1 w... AIM:To determine the roles of high-mobility group box1(HMGB1)in pro-inflammation,host immune response and its potential target for treatment in Aspergillus fumigatus(A.fumigatus)keratitis.METHODS:Expression of HMGB1 was tested in C57 BL/6 normal and infected corneas.Dual immunostaining tested coexpression of HMGB1 with TLR4 or LOX-1.C57 BL/6 mice were pretreated with Box A or PBS and then infected.Clinical scores,polymerase chain reaction,ELISA,and MPO assay were used to assess the disease response.Flow cytometry were used to test the effect of Box A on reactive oxygen species(ROS)expression after A.fumigatus stimulation in polymorphonuclear neutrophilic leukocytes(PMN).C57 BL/6 peritoneal macrophages were pretreated with Box B before A.fumigatus stimulation,and MIP-2,IL-1β,TNF-α,HMGB1 and LOX-1 were measured.Macrophages were pretreated with Box B or Box B combined with Poly(I)(an inhibitor of LOX-1)before stimulating with A.fumigatus,and MIP-2,IL-1β,TNF-α,LOX-1,p38-MAPK,p-p38-MAPK were measured.RESULTS:HMGB1 levels were elevated in C57 BL/6 mice after infection.HMGB1 co-expressed with TLR4,and LOX-1 in infiltrated cells.Box A vs PBS treated C57 BL/6 mice had lower clinical scores and down-regulated corneal HMGB1,MIP-2,IL-1βexpression and neutrophil influx.Box B treatment amplified expression of MIP-2,IL-1β,TNF-α,HMGB1 and LOX-1 that induced by A.fumigatus in macrophage.Compared to the treatment of Box B only,the protein expression of IL-1β,TNF-αshowed inhibition of Box B combined with Poly(I),which also reduced the A.fumigatusevoked protein level of LOX-1 and phosphorylation level of p38-MAPK.The production of A.fumigatus-stimulated ROS was significantly declined after Box A pretreatment in PMN.CONCLUSION:Blocking HMGB1 reduces the disease response in C57 BL/6 mice.HMGB1 can amplify the host immune response through p38-MAPK,and is a target for treatment of A.fumigatus keratitis. 展开更多
关键词 fungal keratitis HMGB1 mice MACROPHAGE polymorphonuclear neutrophilic leukocytes
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Regulation of lipoxygenase-1 and Dectin-1 on interleukin-10 in mouse Aspergillus fumigatus keratitis 被引量:2
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作者 Cheng-Ye Che Ke-Lan Yuan +4 位作者 Gui-Qiu Zhao cui li Jing lin Guo-Qiang Zhu Min liu 《International Journal of Ophthalmology(English edition)》 SCIE CAS 2018年第6期905-909,共5页
AIM: To investigate the regulation of lipoxygenase (LOX)-1 and Dectin-1 on interleukin-10 (IL-10) production in mice with Aspergillus fumigatus (A. fumigatus) keratitis. METHODS: The corneas of C57BL/6 mice we... AIM: To investigate the regulation of lipoxygenase (LOX)-1 and Dectin-1 on interleukin-10 (IL-10) production in mice with Aspergillus fumigatus (A. fumigatus) keratitis. METHODS: The corneas of C57BL/6 mice were pretreated with LOX-1 inhibitor Poly(I) or Dectin-1 siRNA separately before the infection of A. fumigatus. Polymerase chain reaction (PCR) and Western blot were used to detect the expression of IL-10. RESULTS: The mRNA and protein expressions of IL-10 were significantly increased in mice with A. fumigatus keratitis. Compared with the group pretreated with sterile water before infection, Poly(I) pretreatment suppressed IL-10 expression significantly. Compared with the group pretreated with scrambled siRNA before infection, Dectin-1 siRNA pretreatment significantly reduced IL-10 expression in response to A. fumigatus infection. CONCLUSION: LOX-1 and Dectin-1 regulate IL-10 production in mouse A. fumigatus keratitis. 展开更多
关键词 KERATITIS Aspergillus fumigatus DECTIN-1 lipoxygenase- 1 interleukin- 10
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Boxb mediate BALB/c mice corneal inflammation through a TLR4/MyD88-dependent signaling pathway in Aspergillus fumigatus keratitis 被引量:11
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作者 Min liu cui li +6 位作者 Gui-Qiu Zhao Jing lin Cheng-Ye Che Qiang Xu Qian Wang Rui Xu Ya-Wen Niu 《International Journal of Ophthalmology(English edition)》 SCIE CAS 2018年第4期548-552,共5页
AIM: To investigate whether high-mobility group box 1(HMGB1) Boxb exacerbates BALB/c mice corneal immune responses and inflammatory through the Toll-like receptor 4(TLR4)/myeloid differentiation primary response... AIM: To investigate whether high-mobility group box 1(HMGB1) Boxb exacerbates BALB/c mice corneal immune responses and inflammatory through the Toll-like receptor 4(TLR4)/myeloid differentiation primary response 88(My D88)-dependent signaling pathway in Aspergillus fumigatus(A. fumigatus) keratitis.METHODS: The mice corneas were pretreated with phosphate buffer saline(PBS), Boxb before A. fumigatus infection. The abdominal cavity extracted macrophages were pretreated with PBS, Boxb, TLR4 inhibitor(CLI-095), Dimethyl sulfoxide(DMSO) separately before A. fumigatus hyphae stimulation. HMGB1 was detected in normal and infected mice corneas and macrophages by real-time reverse transcriptase polymerase chain reaction(RT-PCR), the TLR4, My D88, interleukin-1β(IL-1β), tumor necrosis factor-α(TNF-α) were detected by Western blot and PCR.RESULTS: In BALB/c mice corneas, the expressions of TLR4, HMGB1, IL-1β, TNF-α were increased after A. fumigatus infection. While pretreatment with Boxb significantly increased the expressions of TLR4, HMGB1, My D88, IL-1β, TNF-α compared with PBS control after infection. In BALB/c mice abdominal cavity extracted macrophages, pretreatment with Boxb increased the expressions of TLR4, HMGB1, My D88, IL-1β, TNF-α, while pretreatment with CLI-095 and Boxb significantly decreased the expressions of TLR4, HMGB1, My D88, IL-1β, TNF-α. CONCLUSION: In A. fumigatus keratitis, Boxb play a proinflammatory role in corneal anti-fungi immune response through the HMGB1-TLR4-My D88 signal pathway. 展开更多
关键词 high-mobility group box 1 Aspergillus fumigatus keratitis Toll-like receptor 4
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新时期河北省粮食综合生产能力现状与对策研究 被引量:1
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作者 张新仕 王亚楠 +3 位作者 王晓夕 王桂荣 崔栗 谭鑫 《农业科技管理》 2024年第2期12-15,29,共5页
保障粮食安全是社会经济发展的根基。文章阐述了河北省粮食产业发展现状,如粮食播种面积趋稳回升、粮食单产稳步增长、粮食总产量呈上升趋势、粮食供给储备总量充足等,分析了河北省粮食生产的制约因素,并从加强高标准农田建设、加强京... 保障粮食安全是社会经济发展的根基。文章阐述了河北省粮食产业发展现状,如粮食播种面积趋稳回升、粮食单产稳步增长、粮食总产量呈上升趋势、粮食供给储备总量充足等,分析了河北省粮食生产的制约因素,并从加强高标准农田建设、加强京津冀合作、强化科学防灾减灾、加强经营主体培育等方面提出了促进河北省粮食生产的对策和建议。 展开更多
关键词 河北省 粮食产业 增产潜力 制约因素 路径研究
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考虑充放电激励机制的电动汽车聚合商参与能量-调频市场多阶段运营策略 被引量:1
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作者 葛乐 王庆园 +3 位作者 王明深 崔莉 贾鹏振 陈析孟 《电力自动化设备》 EI CSCD 北大核心 2024年第6期176-184,共9页
完善电动汽车(EV)主动参与充放电双向互动调频的激励机制,综合考虑EV充放电行为在能量-调频市场中的多阶段交互影响,有利于准确评估聚合商参与电力市场的收益。提出了EV聚合商参与多品种市场的多阶段运营策略,以实现聚合商的运营收益最... 完善电动汽车(EV)主动参与充放电双向互动调频的激励机制,综合考虑EV充放电行为在能量-调频市场中的多阶段交互影响,有利于准确评估聚合商参与电力市场的收益。提出了EV聚合商参与多品种市场的多阶段运营策略,以实现聚合商的运营收益最大化。基于充电行为特征的统计数据,建立单体EV的功率及能量响应边界,并考虑用户参与充电和放电控制的差异性,结合用户自身偏好、电池损耗,提出EV集群的充放电激励机制,并在此基础上建立EV集群功率及能量的响应边界约束。基于EV充放电调节控制的时序性和多阶段市场的调频需求,建立考虑多阶段时序交互的多品种市场运营收益优化模型。结合PJM市场的运营案例,验证所提充放电激励机制和运营策略的有效性,结果表明其能在保障用户充电需求的基础上,有效提升聚合商参与能量-调频市场的收益。 展开更多
关键词 电动汽车 聚合商 能量-调频市场 充放电激励机制 多阶段运营
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NADPH oxidase 2 plays a protective role in experimental Aspergillus fumigatus keratitis in mice through killing fungi and limiting the degree of inflammation 被引量:1
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作者 Qiang Xu Qian Wang +5 位作者 li-Ting Hu Jing lin Nan Jiang Xu-Dong Peng cui li Gui-Qiu Zhao 《International Journal of Ophthalmology(English edition)》 SCIE CAS 2022年第7期1044-1052,共9页
AIM: To explore whether nicotinamide adenine dinucleotide phosphate(NADPH) oxidase 2(NOX2) is expressed in fungal keratitis in mice and investigate its role in this disease.METHODS: NOX2 expression was detected in C57... AIM: To explore whether nicotinamide adenine dinucleotide phosphate(NADPH) oxidase 2(NOX2) is expressed in fungal keratitis in mice and investigate its role in this disease.METHODS: NOX2 expression was detected in C57 BL/6 mice. After testing the inhibitory effect of diphenyleneiodonium chloride(DPI) on NOX2, its impact on clinical performance, myeloperoxidase levels, the number of colonies forming units, the level of H3, the generation of reactive oxygen species(ROS) and the release of cytokines [NF-κB, interleukin-17 A(IL-17 A), interleukin-6(IL-6), tumor necrosis factor-α(TNF-α), Nrf2, IL-10, and TGF-β] were compared. A one-way ANOVA and an unpaired, two-tailed Student’s t-test was used to determine the statistical significance.RESULTS: NOX2 expression was significantly increased after Aspergillus fumigatus injection in corneas and that this increase could be reduced by treatment with DPI. DPI treatment produced more severe inflammation and resulted in higher clinical scores, more neutrophils infiltration, a weakened ability to clear fungi, the release of fewer ROS and the formation of neutrophil extracellular traps. Treatment with DPI increased the expression of the proinflammatory cytokines NF-κB, IL-17 A, IL-6, and TNF-α and decreased the expression of the anti-inflammatory cytokines Nrf2, IL-10 and TGF-β compared to their expression levels without DPI treatment.CONCLUSION: NOX2 plays an important role against Aspergillus fumigatus in the mouse cornea through killing fungi and limiting the degree of inflammation. 展开更多
关键词 NOX2 CORNEA Aspergil us fumigatus MICE
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我国设施园艺病虫害发生特点与绿色防控策略
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作者 吴圣勇 张梦迪 +11 位作者 徐进 王恩东 崔丽 雷仲仁 史晓斌 谢学文 王少丽 王甦 魏书军 马中正 何莉梅 高玉林 《中国生物防治学报》 CSCD 北大核心 2024年第5期1169-1180,共12页
我国设施园艺发展迅速,对促进农业现代化、乡村振兴和都市农业发展具有重要作用。本文结合我国设施园艺产业的发展现状,总结设施环境下病虫害发生特点,并从农业防治、物理防治、生物防治、生态调控、生物技术和科学用药等方面概述了病... 我国设施园艺发展迅速,对促进农业现代化、乡村振兴和都市农业发展具有重要作用。本文结合我国设施园艺产业的发展现状,总结设施环境下病虫害发生特点,并从农业防治、物理防治、生物防治、生态调控、生物技术和科学用药等方面概述了病虫害防治技术,最后对设施园艺病虫害防治提出建议,以期为我国设施园艺的健康可持续发展提供参考。 展开更多
关键词 园艺产业 保护地栽培 病虫害 绿色防控
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胡桃醌F127/TPGS混合胶束的制备及其对溃疡性结肠炎的治疗作用
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作者 崔莉 刘芮奇 +2 位作者 孙娥 钟荣玲 魏兰福 《西北药学杂志》 CAS 2024年第3期70-76,共7页
目的优化胡桃醌普郎尼克F127(F127)/聚乙二醇琥珀酸酯(D-alpha tocopheryl polythylene glycol succinate,TPGS)混合胶束的制备工艺,并对该混合胶束进行表征和体内药效学研究。方法用薄膜分散法制备胡桃醌F127/TPGS混合胶束,以包封率为... 目的优化胡桃醌普郎尼克F127(F127)/聚乙二醇琥珀酸酯(D-alpha tocopheryl polythylene glycol succinate,TPGS)混合胶束的制备工艺,并对该混合胶束进行表征和体内药效学研究。方法用薄膜分散法制备胡桃醌F127/TPGS混合胶束,以包封率为指标,用单因素和3因素3水平响应面实验优化胡桃醌混合胶束的制备工艺。用透射电镜和马尔文粒度仪对混合胶束进行表征。用透析袋研究其体外释放度。通过疾病活动指数(DAI)和结肠HE切片研究胡桃醌F127/TPGS混合胶束体内抗溃疡性结肠炎作用。结果胡桃醌F127/TPGS混合胶束的最佳制备工艺:药物∶载体=1∶50、F127∶FPGS=4∶5、水化体积为10 mL、水化时间为1 h、乙醇体积为1 mL。对最优处方进行验证,得到胡桃醌的平均包封率为90.53%,与预测值(85.32%)偏差较小。透射电镜下混合胶束呈球形,形态完整且分布均匀。平均电位为(−3.86±3.05)mV,平均粒径为(13.91±0.15)nm,PDI为(0.092±0.009)。体外释放度实验结果显示,胡桃醌F127/TPGS混合胶束的累积释放度大于胡桃醌混悬液。胡桃醌F127/TPGS混合胶束干预后,小鼠DAI值下降,结肠组织病理损伤得到缓解。结论经响应面优化的胡桃醌F127/TPGS混合胶束制备工艺条件稳定,且可增加胡桃醌溶解度,增强胡桃醌抗溃疡性结肠炎的作用。 展开更多
关键词 胡桃醌 Box-Behnken响应面法 F127 聚乙二醇琥珀酸酯 混合胶束
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