Carbohydrate represents an important part of the soil labile organic carbon pool. Water soluble carbohydrate drives the C cycle in forest soil by affecting microbial activity and hot water extractable car- bohydrate i...Carbohydrate represents an important part of the soil labile organic carbon pool. Water soluble carbohydrate drives the C cycle in forest soil by affecting microbial activity and hot water extractable car- bohydrate is thought related to soil carbon sequestration due to the asso- ciation with soil aggregation. In a temperate forest region of northeast China, Changbai Mountain, we investigated the abundance, spatial dis- tribution, and seasonal dynamics of cool and hot-water extractable car- bohydrate in soils under mixed broad-leaved Korean pine forest. The concentrations of cool-water extractable carbohydrate （CWECH） in three soil layers （0-5, 5-10, 10-20 cm） ranged from 4.1 to 193.3 g.kg-1 dry soil, decreasing rapidly with soil depth. On an annual average, the CWECH concentrations in soils at depths of 5-10 and 10-20 cm were 54.2% and 24.0%, respectively, of that in the 0-5 cm soil layer. CWECH showed distinct seasonal dynamics with the highest concentrations in early spring, lowest in summer, and increasing concentrations in autumn. Hot-water extractable carbohydrate （HWECH） concentrations in three soil layers ranged from 121.4 to 2026.2 g.kgq dry soil, which were about one order of magnitude higher than CWECH. The abundance of HWECH was even more profile-dependent than CWECH, and decreased more rapidly with soil depth. On an annual average, the HWECH concentration in soils 10-20 cm deep was about one order of magnitude lower than that in the top 0-5 cm soil. The seasonality of HWECH roughly tracked that of CWECH but with seasonal fluctuations of smaller amplitude. The car- bohydrate concentrations in cool/hot water extracts of soil were positively correlated with UV254 and UV2s0 of the same solution, which has implications for predicting the leaching loss of water soluble organic carbon.展开更多
采用土壤细菌16S r DNA高通量测序方法研究了大兴安岭汗马自然保护区落叶松林不同演替阶段的土壤细菌群落结构和功能.结果表明:不同演替阶段落叶松林土壤细菌优势门为变形菌门、酸杆菌门、疣微菌门、拟杆菌门、放线菌门、浮霉菌门和绿...采用土壤细菌16S r DNA高通量测序方法研究了大兴安岭汗马自然保护区落叶松林不同演替阶段的土壤细菌群落结构和功能.结果表明:不同演替阶段落叶松林土壤细菌优势门为变形菌门、酸杆菌门、疣微菌门、拟杆菌门、放线菌门、浮霉菌门和绿弯菌门,随着演替的进行,酸杆菌门相对丰度逐渐增加,绿弯菌门相对丰度逐渐减少,优势门相对丰度在不同演替阶段不同.细菌群落α多样性在不同演替阶段间无显著差异,但其群落结构分别在落叶松幼龄林与中龄林、幼龄林与过熟林、近熟林与成熟林之间存在显著差异.土壤氧化还原电位、土壤p H和有效磷是影响细菌群落结构的主要环境因素,其中土壤氧化还原电位对微生物群落结构影响最大.随着演替的进行,细菌参与的固氮作用、反硝化作用、氨氧化作用、木质素降解作用呈逐渐减弱的趋势,硫酸盐异化还原作用呈先降后升的趋势,碳固定作用呈先升后降的趋势,碱性磷酸酶没有明显的变化规律,影响土壤功能的主要因素有土壤有效磷和氧化还原电位等.展开更多
基金supported by made possible through National Key Basic Research Foundation, China (grants 2011CB403202)the National Natural Science Foundation of China (40930107)Program for Changjiang Scholars and Innovative Research Team in University (IRT1054)
文摘Carbohydrate represents an important part of the soil labile organic carbon pool. Water soluble carbohydrate drives the C cycle in forest soil by affecting microbial activity and hot water extractable car- bohydrate is thought related to soil carbon sequestration due to the asso- ciation with soil aggregation. In a temperate forest region of northeast China, Changbai Mountain, we investigated the abundance, spatial dis- tribution, and seasonal dynamics of cool and hot-water extractable car- bohydrate in soils under mixed broad-leaved Korean pine forest. The concentrations of cool-water extractable carbohydrate （CWECH） in three soil layers （0-5, 5-10, 10-20 cm） ranged from 4.1 to 193.3 g.kg-1 dry soil, decreasing rapidly with soil depth. On an annual average, the CWECH concentrations in soils at depths of 5-10 and 10-20 cm were 54.2% and 24.0%, respectively, of that in the 0-5 cm soil layer. CWECH showed distinct seasonal dynamics with the highest concentrations in early spring, lowest in summer, and increasing concentrations in autumn. Hot-water extractable carbohydrate （HWECH） concentrations in three soil layers ranged from 121.4 to 2026.2 g.kgq dry soil, which were about one order of magnitude higher than CWECH. The abundance of HWECH was even more profile-dependent than CWECH, and decreased more rapidly with soil depth. On an annual average, the HWECH concentration in soils 10-20 cm deep was about one order of magnitude lower than that in the top 0-5 cm soil. The seasonality of HWECH roughly tracked that of CWECH but with seasonal fluctuations of smaller amplitude. The car- bohydrate concentrations in cool/hot water extracts of soil were positively correlated with UV254 and UV2s0 of the same solution, which has implications for predicting the leaching loss of water soluble organic carbon.
文摘采用土壤细菌16S r DNA高通量测序方法研究了大兴安岭汗马自然保护区落叶松林不同演替阶段的土壤细菌群落结构和功能.结果表明:不同演替阶段落叶松林土壤细菌优势门为变形菌门、酸杆菌门、疣微菌门、拟杆菌门、放线菌门、浮霉菌门和绿弯菌门,随着演替的进行,酸杆菌门相对丰度逐渐增加,绿弯菌门相对丰度逐渐减少,优势门相对丰度在不同演替阶段不同.细菌群落α多样性在不同演替阶段间无显著差异,但其群落结构分别在落叶松幼龄林与中龄林、幼龄林与过熟林、近熟林与成熟林之间存在显著差异.土壤氧化还原电位、土壤p H和有效磷是影响细菌群落结构的主要环境因素,其中土壤氧化还原电位对微生物群落结构影响最大.随着演替的进行,细菌参与的固氮作用、反硝化作用、氨氧化作用、木质素降解作用呈逐渐减弱的趋势,硫酸盐异化还原作用呈先降后升的趋势,碳固定作用呈先升后降的趋势,碱性磷酸酶没有明显的变化规律,影响土壤功能的主要因素有土壤有效磷和氧化还原电位等.
