Lectins are sugar-specific binding proteins or glycoproteins that play important physiological roles in cellular recognition and regulation. And they are also valuable in medicine and pharmacy. Tubeworm is the represe...Lectins are sugar-specific binding proteins or glycoproteins that play important physiological roles in cellular recognition and regulation. And they are also valuable in medicine and pharmacy. Tubeworm is the representative species around the hydrothermal vent in the deep sea. They have developed unique mechanisms to adapt to the harsh environment. In this study, a 1 092 bp cDNA, designed as rpgal, was first cloned and characterized from the tubeworm Ridgeia piscesae. Sequence analysis showed that RPGAL had low homology with the known galectin. And it had two homologous carbohydrate-recognition domains, which is the characteristic of the tandem-repeat type galectins. The RPGAL was successfully recombinant expressed in Escherichia coli and purified. Analysis of biological activity revealed that RPGAL was metal ion independent and it could agglutinate all the vertebrate erythrocytes tested. It was stable at 10-50℃ and pH 5-10. And the hemagglutinating activity of RPGAL was strongly inhibited by D-Lactose and lipopolysaccharide. Although RPGAL had no effect on the microorganisms tested, it showed anti-tumor activity towards HeLa cells and HT1080 cells, which was accomplished by apoptosis. The study demonstrated that RPGAL was a novel galectin and provided a potential candidate for therapy of anti-tumor.展开更多
[ Objective] The paper was to understand the natural infection status of avian leukemia in some indigenous chicken breeds of China. [ Method ] Using ELISA assay and virus isolation method, epidemiological investigatio...[ Objective] The paper was to understand the natural infection status of avian leukemia in some indigenous chicken breeds of China. [ Method ] Using ELISA assay and virus isolation method, epidemiological investigation of ALV-AB and ALV-J avian leukemia of 10 indigenous chicken breeds were conducted. ALV dynamics were monitored in F2 generation of four chicken lines. ALV pollution of attenuated live vaccines used in raising process was also inspected through sampling method. [ Result] The positive rate of ALV-P27 antigen was 0 -62.1% ; the positive rate of ALV-AB antibody was 0 -25.0% ; the positive rate of ALV-J antibody was 0 - 59.0% ; the positive rate of virus isolation was 0 - 22.0%. The positive rate of ALV-P＇27 antigen in 1 -day-old chick mecunium of four lines was 6.0% - 67.0%. The positive rate of virus isolation in 6-week-old chickens was 2.0% - 34.3%. Two kinds of vaccines from two hatches pro- duced by a manufacturer were polluted by ALV. [ Conclusion] Most indigenous chickens were infected by ALV. There were great differences among different breeds of indigenous chicken, which might be related to ALV genetic resistance of different indigenous chickens. The ALV positive rates of F： chicks were slightly enhanced in some lines, which might be related to vaccine pollution.展开更多
基金The Major State Basic Research Development Program of China(973 Program)under contract No.2015CB755906China Ocean Mineral Resources R&D Association under contract No.DYXM-115-02-2-16
文摘Lectins are sugar-specific binding proteins or glycoproteins that play important physiological roles in cellular recognition and regulation. And they are also valuable in medicine and pharmacy. Tubeworm is the representative species around the hydrothermal vent in the deep sea. They have developed unique mechanisms to adapt to the harsh environment. In this study, a 1 092 bp cDNA, designed as rpgal, was first cloned and characterized from the tubeworm Ridgeia piscesae. Sequence analysis showed that RPGAL had low homology with the known galectin. And it had two homologous carbohydrate-recognition domains, which is the characteristic of the tandem-repeat type galectins. The RPGAL was successfully recombinant expressed in Escherichia coli and purified. Analysis of biological activity revealed that RPGAL was metal ion independent and it could agglutinate all the vertebrate erythrocytes tested. It was stable at 10-50℃ and pH 5-10. And the hemagglutinating activity of RPGAL was strongly inhibited by D-Lactose and lipopolysaccharide. Although RPGAL had no effect on the microorganisms tested, it showed anti-tumor activity towards HeLa cells and HT1080 cells, which was accomplished by apoptosis. The study demonstrated that RPGAL was a novel galectin and provided a potential candidate for therapy of anti-tumor.
基金Supported by Special Fund for Agro-scientific Research in the Public Interest(201203055)Agricultural Three-new Engineering Project of Jiangsu Province(SXGC[2014]288)+1 种基金Natural Science Foundation of Jiangsu Province(20151317)Science and Technology Project of Yangzhou City(YZ2014144)
文摘[ Objective] The paper was to understand the natural infection status of avian leukemia in some indigenous chicken breeds of China. [ Method ] Using ELISA assay and virus isolation method, epidemiological investigation of ALV-AB and ALV-J avian leukemia of 10 indigenous chicken breeds were conducted. ALV dynamics were monitored in F2 generation of four chicken lines. ALV pollution of attenuated live vaccines used in raising process was also inspected through sampling method. [ Result] The positive rate of ALV-P27 antigen was 0 -62.1% ; the positive rate of ALV-AB antibody was 0 -25.0% ; the positive rate of ALV-J antibody was 0 - 59.0% ; the positive rate of virus isolation was 0 - 22.0%. The positive rate of ALV-P＇27 antigen in 1 -day-old chick mecunium of four lines was 6.0% - 67.0%. The positive rate of virus isolation in 6-week-old chickens was 2.0% - 34.3%. Two kinds of vaccines from two hatches pro- duced by a manufacturer were polluted by ALV. [ Conclusion] Most indigenous chickens were infected by ALV. There were great differences among different breeds of indigenous chicken, which might be related to ALV genetic resistance of different indigenous chickens. The ALV positive rates of F： chicks were slightly enhanced in some lines, which might be related to vaccine pollution.
