NONINVASIVE OPTICAL IMAGING OF STAPHYLOCOCCUS AUREUS INFECTION IN VIVO USING AN ANTIMICROBIAL PEPTIDE FRAGMENT BASED NEAR-INFRARED FLUORESCENT PROBES

The diagnosis of bacterial infections remains a major challenge in medicine.Optical imaging of bacterial infection in living animals is usually conducted with genetic reporters such as lightemitting encymes or fluorescent proteins.However,there are many circumstances where genetic reporters are not applicable,and there is an urgent need for exogenous synthetic probes that can selectively target bacteria.Optical imaging of bacteria in vivo is much less developed than methods such as ndioimaging and MRI.Furthermore near infrared(NIR)dyes with emision wavelengths in the region of 650-900 rm can propagate through two or more centimeters of tissue and may enable deeper tisue imaging if sensitive detection techniques are employed.Here we constructed an antimicrobial peptide fragment UBI29-41-based near infrared fuorescent imaging probe.The probe is composed of UBI2941 conjugated to a near infraured dye ICG Der-02.UBI29-41 is a cationic antimicrobial peptide that targets the anionic surfaces of bacterial cells.The probe allows detection of Staphylococcus aureus infection(5×10^(7)cells)in a mouse local infection model using whole animal nearinfrared fuorescence imaging.Furthermore,we demonstrate that the UBI29-41-based imaging probe can selectively accumulate within bacteria.The significantly higher accumulation in bacterial infection suggests that UBI29-41-based imaging probe may be a promising imaging agent to detect bacterial infections.

A Novel Method of Auxiliary Power Supply Used in Wide-Range High Voltage Input DC-DC Converter

In high voltage input DC-DC converter, auxiliary winding of isolation transformer is usually used to supply power for control circuit. Due to the wide-range of input voltage, the variable output voltage of auxiliary winding will cause a series of problems, such as variable drive pulse amplitude, increased driver switching devices loss and drive transformer core saturation. This paper analyzes the influence of variable output voltage of auxiliary winding in detail. A novel method is proposed to solve the problem of large variation range of auxiliary winding output voltage, which is adding a buck converter between the auxiliary winding and the control circuit. A dual switch forward convert has been designed with 300 V - 800 V input and 24 V/5 A output. The results show that this method is effective by comparing the different results of using buck converter or not.

Study on Isolation and Degradation Characteristics of an Aniline-degrading Strain

[Objective] The research aimed to screen out an efficient aniline-degrading strain and study its degradation characteristics. [Method] By domesticated enrichment culture, an efficient aniline-degrading strain named as DA-K was isolated from activated sludge sample collected from a chemical plant in Henan. DA-K could use aniline as the sole carbon and nitrogen sources. The strain was carried out physiological and biochemi- cal identification, and its biological degradation characteristics were studied. [ Result] DA-K strain was G - and red-shaped, and its colony color was off-white. It was initially determined as Acinetobacter sp. By measuring, the optimum growth temperature and pH for DA-K were respectively 30 ~C and 6.0. DA-K strain could grow well in inorganic salt medium with aniline of 2 500 mg/L. After shaking for 96 h under the conditions of pH 6.0, 30 ℃, 180 r/min and 1 000 mg/L of aniline, the degradation rate of the aniline by DA-K could reach nearly 80%. [ Conclusion] The DA-K strain had a higher aniline degradation efficiency and actual treatment capability of the aniline wastewater, which laid foundation for establishing gene engineerincl strain.

Identification and characterization of human hematopoietic mesoderm

The embryonic mesoderm comprises heterogeneous cell subpopulations with distinct lineage biases.It is unclear whether a bias for the human hematopoietic lineage emerges at this early developmental stage.In this study,we integrated single-cell transcriptomic analyses of human mesoderm cells from embryonic stem cells and embryos,enabling us to identify and define the molecular features of human hematopoietic mesoderm(HM)cells biased towards hematopoietic lineages.We discovered that BMP4 plays an essential role in HM specification and can serve as a marker for HM cells.Mechanistically,BMP4 acts as a downstream target of HDAC1,which modulates the expression of BMP4 by deacetylating its enhancer.Inhibition of HDAC significantly enhances HM specification and promotes subsequent hematopoietic cell differentiation.In conclusion,our study identifies human HM cells and describes new mechanisms for human hematopoietic development.

共底贮箱内液体晃动动力学特性研究及实验验证

本文通过数值方法、实验方法和等效力学模型方法研究了共底贮箱内液体晃动的动力学特性.研究结果表明,一阶晃动频率随着充液比的增加而增大.然而,二阶晃动频率随着充液比的增加而减小.通过两个算例来验证改进的移动脉动球模型,其结果与数值和实验结果非常吻合.此外,通过CFD研究了CBH槽内非线性液体晃动的复杂多模态或高阶模态特征,结果表明,在强迫激励下能明显观察到高阶晃动模态.最后,通过实验揭示了共底贮箱内的旋转晃动和对称旋转晃动现象.发现由于激励振幅和激励频率的数值变化,旋转晃动会变得不稳定,并发展为对称旋转晃动.对称旋转晃动将以特定的节奏间歇性地开始,这取决于激励的频率.

Numerical Simulation of Flow and Heat Transfer from Slot Jets Impinging on a Cylindrical Convex Surface

Flow and heat transfer characteristics of slot jets impingement to a cylindrical convex surface are numerically investigated.Suitable turbulence models have been determined through comparison with the experimental data.Flow structures are described and impingement heat transfer characteristics are discussed.The effects of Re,H/B and D/B on single-slot jets impingement heat transfer are analyzed and heat transfer characteristics of multiple-slot jets are investigated.The results show that:Gas flows along the convex surface and boundary layer separation occurs in both single and multiple-slot jets impingement.A maximum stagnation Nu appears at H/B=8 and the local Nu decreases with increasing H/B in the region far away from the stagnation.The Nu in the stagnation region decreases with increasing D/B but the Nu is nearly the same in the region far away from the stagnation.Pressure gradient is an important factor on heat transfer enhancement.Correlations of the Num for single-slot,double-slot and quadric-slot jets impinging on a convex surface are obtained.It indicates the effects of Re and D/B on Num could become more important in less slot jets impingement.