Using laser scanning confocal microscopy, we have found that the in cells loaded with fluo-3/AM, highest intracellular Ca(2+) in the perinuclear region is associated with the Golgi apparatus. The spatiotemporal subcel...Using laser scanning confocal microscopy, we have found that the in cells loaded with fluo-3/AM, highest intracellular Ca(2+) in the perinuclear region is associated with the Golgi apparatus. The spatiotemporal subcellu lar distribution of Ca(2+) in living human fibroblasts exposing to calcium-free medium in response to agonists has been investigated. PDGF, which releases Ca(2+) from intracellular stores by inositol(1, 4, 5)-trisphosphate pathway,produced a biphasic transient rise in intracellular calcium.The initial rise was resulted from a direct release of calcium from the Golgi apparatus. Calcium could be also released from and reaccumulated into the Golgi apparatus by the stimulation of thapsigargin, an inhibitor of the Ca(2+) transport ATPase of intracellular calcium store. Permeablizing the plasma membrane by 10 μM digitonin resulted in the calcium release from the Golgi apparatus and depletion of the internal calcium store. These results suggest that the Golgi apparatus plays a role in Ca(2+) regulation in signal transduction.展开更多
In this paper, the quaternary Delsarte-Goethals code D(m,δ) and its dual code G(m,δ) are discussed. The type and the trace representation are given for D(m,δ), while the type and the minimum Lee weight are determin...In this paper, the quaternary Delsarte-Goethals code D(m,δ) and its dual code G(m,δ) are discussed. The type and the trace representation are given for D(m,δ), while the type and the minimum Lee weight are determined for G(m,δ). The shortened codes of D(m,δ) and G(m,δ) are proved to be 4-cyclic. The binary image of D(m,δ) is proved to be the binary Delsarte-Goethals code DG(m+1,δ),and the essential difference between the binary image of G(m,δ) and the binary Goethals-Delsarte codeGD(m+1,δ) is exhibited. Finally, the decoding algorithms of D■(m,δ) and G(m,δ) are discussed.展开更多
文摘Using laser scanning confocal microscopy, we have found that the in cells loaded with fluo-3/AM, highest intracellular Ca(2+) in the perinuclear region is associated with the Golgi apparatus. The spatiotemporal subcellu lar distribution of Ca(2+) in living human fibroblasts exposing to calcium-free medium in response to agonists has been investigated. PDGF, which releases Ca(2+) from intracellular stores by inositol(1, 4, 5)-trisphosphate pathway,produced a biphasic transient rise in intracellular calcium.The initial rise was resulted from a direct release of calcium from the Golgi apparatus. Calcium could be also released from and reaccumulated into the Golgi apparatus by the stimulation of thapsigargin, an inhibitor of the Ca(2+) transport ATPase of intracellular calcium store. Permeablizing the plasma membrane by 10 μM digitonin resulted in the calcium release from the Golgi apparatus and depletion of the internal calcium store. These results suggest that the Golgi apparatus plays a role in Ca(2+) regulation in signal transduction.
文摘In this paper, the quaternary Delsarte-Goethals code D(m,δ) and its dual code G(m,δ) are discussed. The type and the trace representation are given for D(m,δ), while the type and the minimum Lee weight are determined for G(m,δ). The shortened codes of D(m,δ) and G(m,δ) are proved to be 4-cyclic. The binary image of D(m,δ) is proved to be the binary Delsarte-Goethals code DG(m+1,δ),and the essential difference between the binary image of G(m,δ) and the binary Goethals-Delsarte codeGD(m+1,δ) is exhibited. Finally, the decoding algorithms of D■(m,δ) and G(m,δ) are discussed.
