[目的]明确痰瘀同治方对心肌缺血区和动脉粥样硬化(atherosclerosis,AS)斑块内血管新生的双向调节作用,探讨自噬在其中的调控机制。[方法]选取载脂蛋白E基因敲除(Apolipoprotein E gene knockout,ApoE-/-)小鼠20只,随机分为模型对照组...[目的]明确痰瘀同治方对心肌缺血区和动脉粥样硬化(atherosclerosis,AS)斑块内血管新生的双向调节作用,探讨自噬在其中的调控机制。[方法]选取载脂蛋白E基因敲除(Apolipoprotein E gene knockout,ApoE-/-)小鼠20只,随机分为模型对照组和痰瘀同治(Tanyu Tongzhi,TYTZ)组,每组10只,饲喂高脂饲料;另取C57BL/6J小鼠10只为正常对照组,饲喂基础饲料。饲喂4周后,模型对照组和TYTZ组皮下注射异丙肾上腺素针100mg/kg,连续2d,正常对照组皮下注射等量0.9%氯化钠注射液。造模成功后4周,TYTZ组给予TYTZ方流浸膏2145mg/(kg·d),以蒸馏水稀释成0.4mL灌胃,模型对照组和正常对照组给予等量蒸馏水灌胃。给药6周后,用CD31免疫组化染色观察心肌缺血区和主动脉的新生血管密度。Western blot检测心肌组织和主动脉微管相关蛋白1轻链3(microtubule-associated protein 1 light chain 3,LC3)Ⅱ/Ⅰ蛋白表达。[结果]免疫组化染色结果显示,模型对照组心肌组织和主动脉组织CD31表达均显著高于正常对照组(P<0.05);TYTZ组心肌组织CD31表达明显高于模型对照组(P<0.05),而主动脉组织CD31表达则明显低于模型对照组(P<0.05)。Western blot结果显示,模型对照组心肌组织和主动脉组织LC3Ⅱ/Ⅰ蛋白表达水平均明显高于正常对照组(P<0.05);TYTZ组心肌组织LC3Ⅱ/Ⅰ蛋白表达水平显著高于模型对照组(P<0.05),而主动脉组织LC3Ⅱ/Ⅰ蛋白表达则明显低于模型对照组(P<0.05)。[结论]TYTZ方在抑制AS斑块内血管新生的同时能够促进心肌缺血区的血管新生,该方对自噬水平的双向调节作用可能是其中的调控机制。展开更多
The Yantai Development Zone belongs to Yantai City in Shandong Province, one of China’s first 14 open coastal cities. The district is situated on the blue sea northward and nestles among the green pine hills southwar...The Yantai Development Zone belongs to Yantai City in Shandong Province, one of China’s first 14 open coastal cities. The district is situated on the blue sea northward and nestles among the green pine hills southward. Its construction area is 20 square kilometers, half of which has been built into a new industrial district, following ten years of construction. Mr. Hu Ping, Director of the Special Zone Office of the State Council, has inspected the area several times in recent years. He said, "Among all the development zones in China, Yantai is next only to Dalian in terms of construction."展开更多
In recent years, Yentai City has witnessed an annual growth of 30 percent in exports since it began to handle its own products. As a result it earned US$220 million in 1993. Xu Zhihqiang, director of the Yentai Foreig...In recent years, Yentai City has witnessed an annual growth of 30 percent in exports since it began to handle its own products. As a result it earned US$220 million in 1993. Xu Zhihqiang, director of the Yentai Foreign Trade Bureau, said that after some of the foreign trade corporations in Yentai obtained the right to handle exports in1989, they sent over 300 trade展开更多
文摘The Yantai Development Zone belongs to Yantai City in Shandong Province, one of China’s first 14 open coastal cities. The district is situated on the blue sea northward and nestles among the green pine hills southward. Its construction area is 20 square kilometers, half of which has been built into a new industrial district, following ten years of construction. Mr. Hu Ping, Director of the Special Zone Office of the State Council, has inspected the area several times in recent years. He said, "Among all the development zones in China, Yantai is next only to Dalian in terms of construction."
文摘In recent years, Yentai City has witnessed an annual growth of 30 percent in exports since it began to handle its own products. As a result it earned US$220 million in 1993. Xu Zhihqiang, director of the Yentai Foreign Trade Bureau, said that after some of the foreign trade corporations in Yentai obtained the right to handle exports in1989, they sent over 300 trade
