Correlation of SphK1, FAK, Musashi 1 and CA199 expression with angiogenesis and epithelial-mesenchymal transition in surgically removed colon cancer lesions

Objective: To investigate the correlation of SphK1, FAK, Musashi 1 and CA199 expression with angiogenesis and epithelial-mesenchymal transition in surgically removed colon cancer lesions. Methods: A total of 60 patients with colon cancer who underwent radical operation in our hospital between August 2015 and August 2017 were selected, intraoperative colon cancer tissue samples were collected as colon cancer group, and normal tissue specimens adjacent to carcinoma were collected as adjacent tissue group. Fluorescence quantitative PCR was adopted to determine the expression levels of SphK1, FAK, Musashi 1, CA199 as well as the genes related to angiogenesis and epithelial-mesenchymal transition in colon tissues with different properties. Results: SphK1, FAK, Musashi 1 and CA199 mRNA expression in colon cancer group were higher than those in adjacent tissue group;angiogenesis-related genes ANGPTL4, Apelin-13, DLL1, VEGF and HIF-α mRNA expression were higher than those in adjacent tissue group whereas TSP-1 mRNA expression was lower than that in adjacent tissue group;epithelial-mesenchymal transition-related gene E-cadherin mRNA expression was lower than that in adjacent tissue group whereas Vimentin, N-cadherin, Twist and Snail mRNA expression were higher than those in adjacent tissue group. Correlation analysis showed that the SphK1, FAK, Musashi 1 and CA199 expression in colon cancer tissues were directly correlated with the angiogenesis genes and epithelial-mesenchymal transition genes. Conclusion: SphK1, FAK, Musashi 1 and CA199 genes are abnormally expressed in colon cancer tissues and their expression levels are directly correlated with tumor angiogenesis and epithelial-mesenchymal transition process.

Bone metabolism and trauma degree of magnetic-guided intramedullary nail fixation for femoral shaft fracture

Objective:To explore the effect of magnetic-guided intramedullary nail fixation on bone metabolism and trauma degree in patients with femoral shaft fracture.Methods: A total of 128 patients with femoral shaft fractures who received surgical treatment in the hospital between April 2016 and November 2017 were divided into control group (n=64) and study group (n=64) according to the random number table method. Control group received the traditional intramedullary nail treatment, and study group received magnetic-guided intramedullary nail treatment. The differences in serum levels of bone metabolism indexes and inflammatory factors were compared between the two groups 48 h after surgery.Results: 48 h after surgery, serum bone formation indexes BGP, PⅠNP, PⅠCP and BAP levels of study group were higher than those of control group whereas bone resorption indexesβ-CTX, TRACP5b and NTX levels were lower than those of control group;serum inflammatory factors TGF-β, hs-CRP, IL-1β, IL-6 and IL-17 levels were lower than those of control group.Conclusion:Compared with traditional intramedullary nail therapy, magnetic-guided intramedullary nail fixation can more effectively balance the bone metabolism status and reduce the fracture end trauma in patients with femoral shaft fracture.