Objective:The study objective was to investigate the effects of luteinizing hormone(LH)supplementation on ovarian response and assisted reproductive technology(ART)outcomes in in vitro fertilization/intracytoplasmic s...Objective:The study objective was to investigate the effects of luteinizing hormone(LH)supplementation on ovarian response and assisted reproductive technology(ART)outcomes in in vitro fertilization/intracytoplasmic sperm injection cycles with a gonadotropin(Gn)-releasing hormone antagonist protocol.Methods:This is a meta-analysis,and nine published randomized controlled trials(1,685 patients)were included.Continuous data were extracted in the form of mean±standard deviation and population size,whereas dichotomous data were extracted in the form of odds ratio.Results:The total amount of follicle-stimulating hormone(FSH)used,the duration of stimulation(DOS),the number of eggs in MII stage,the total number of formed embryos,the clinical pregnancy rate,and live birth rates were similar between groups,but the estrogen level on the day of human chorionic Gn(hCG)administration was slightly higher in the LH supplementation group.On subgroup analysis,it was reported that the addition of LH could significantly increase estrogen levels on the day of hCG administration in patients older than 35 years,and LH supplementation starting on the day of FSH administration may slightly extend the DOS.Moreover,regardless of the timing of LH supplementation,an increase in estrogen levels was found on the day of hCG administration.Conclusions:LH supplementation of an antagonist protocol increases estrogen levels on the day of hCG administration,but does not increase the number of mature oocytes retrieved,and also fails to improve ART outcomes.展开更多
Objective To investigate the expression and the distribution of Dickkopf-likel （Dkkll） protein during the development of mouse testis. Methods Testes eDNA samples from BALB/c mice in different postnatal days were hy...Objective To investigate the expression and the distribution of Dickkopf-likel （Dkkll） protein during the development of mouse testis. Methods Testes eDNA samples from BALB/c mice in different postnatal days were hybridized with mouse whole genome affymetrix chip to screen the spermatogenesisrelated genes. The characteristics of the selected genes were analyzed by various bioinformatics tools. The mRNA expression of Dkkll at different stages of testis development and different tissues in mouse were analyzed by RT-PCR. The protein localization of Dkkll in mouse testis was assesed by immunohistoehemistry. Results By analyzing the gene chip signals of mouse testis aged 4 d, 9 d, 18 d, 35 d, 54 d and 6 months, Dkkll was identified with a differential expression in the develop- mental stages of testis. RT-PCR analysis showed that the expression of Dkkll mRNA was firstly detected on 15 d testis tissue and gradually upregulated during the testis developing to the adult stage. The Dkkll protein was predominantly located in spermatocytes and round spermatids in mouse testis. Conclusion The expression of Dkkll is gradually upregulated during the development of mouse testis and corresponds to the mouse spermatogenesis. It may play a critical role in male mammalian spermatogenesis.展开更多
文摘Objective:The study objective was to investigate the effects of luteinizing hormone(LH)supplementation on ovarian response and assisted reproductive technology(ART)outcomes in in vitro fertilization/intracytoplasmic sperm injection cycles with a gonadotropin(Gn)-releasing hormone antagonist protocol.Methods:This is a meta-analysis,and nine published randomized controlled trials(1,685 patients)were included.Continuous data were extracted in the form of mean±standard deviation and population size,whereas dichotomous data were extracted in the form of odds ratio.Results:The total amount of follicle-stimulating hormone(FSH)used,the duration of stimulation(DOS),the number of eggs in MII stage,the total number of formed embryos,the clinical pregnancy rate,and live birth rates were similar between groups,but the estrogen level on the day of human chorionic Gn(hCG)administration was slightly higher in the LH supplementation group.On subgroup analysis,it was reported that the addition of LH could significantly increase estrogen levels on the day of hCG administration in patients older than 35 years,and LH supplementation starting on the day of FSH administration may slightly extend the DOS.Moreover,regardless of the timing of LH supplementation,an increase in estrogen levels was found on the day of hCG administration.Conclusions:LH supplementation of an antagonist protocol increases estrogen levels on the day of hCG administration,but does not increase the number of mature oocytes retrieved,and also fails to improve ART outcomes.
基金supported by the Medical Research Foundation of Guangdong Province(B2014426)the Qingyuan Foundation of Science&Technology(2012B011204127)+1 种基金the National Natural Science Foundation of China(No.81170613,No.81270740)Shenzhen Basic Research Funds for Distinguished Young Scientists(JC201005260216A)
文摘Objective To investigate the expression and the distribution of Dickkopf-likel （Dkkll） protein during the development of mouse testis. Methods Testes eDNA samples from BALB/c mice in different postnatal days were hybridized with mouse whole genome affymetrix chip to screen the spermatogenesisrelated genes. The characteristics of the selected genes were analyzed by various bioinformatics tools. The mRNA expression of Dkkll at different stages of testis development and different tissues in mouse were analyzed by RT-PCR. The protein localization of Dkkll in mouse testis was assesed by immunohistoehemistry. Results By analyzing the gene chip signals of mouse testis aged 4 d, 9 d, 18 d, 35 d, 54 d and 6 months, Dkkll was identified with a differential expression in the develop- mental stages of testis. RT-PCR analysis showed that the expression of Dkkll mRNA was firstly detected on 15 d testis tissue and gradually upregulated during the testis developing to the adult stage. The Dkkll protein was predominantly located in spermatocytes and round spermatids in mouse testis. Conclusion The expression of Dkkll is gradually upregulated during the development of mouse testis and corresponds to the mouse spermatogenesis. It may play a critical role in male mammalian spermatogenesis.
