Great uncertainty exists in reservoir models built for blocks where well spacing is uneven or large. The uncertainty in reservoir models can be significantly reduced by using Coordinate Cokriging Sequential Gaussian S...Great uncertainty exists in reservoir models built for blocks where well spacing is uneven or large. The uncertainty in reservoir models can be significantly reduced by using Coordinate Cokriging Sequential Gaussian Simulation technology, in combination with the restriction of seismic characteristic data. Satisfactory reservoir parameter interpolation results, which are more accurate than those derived only from borehole data, are obtained, giving rise to a reasonable combination of widespread and dense-sampled seismic (soft data) data with borehole data (hard data). A significant effect has been made in reservoir parameter modeling in the Chegu 201 block of the Futai Oilfield by using this technology.展开更多
The promoter region (BCSP666) of b-conglycinin a-subunit gene from the genomic DNA of soybean Jilin 43 was isolatedby PCR method. Sequencing analysis showed that the cloned fragment BCSP666 had the similar structure ...The promoter region (BCSP666) of b-conglycinin a-subunit gene from the genomic DNA of soybean Jilin 43 was isolatedby PCR method. Sequencing analysis showed that the cloned fragment BCSP666 had the similar structure to the soybeanseed-specific promoter b-conglycinin a'-subunit gene promoter and b-conglycinin b-subunit gene promoter, and it alsocontains many motifs that contribute to the seed-specific promoter activity. Based on this sequencing analysis, wededuced that promoter fragment BCSP666 had the seed-sepecific promoter activity. And then we constructed the seed-specific expression vector pBMI666 with the promoter fragment BCSP666 and D6-fatty acid desaturase gene fromMortierella isabellina. The D6-fatty acid desaturase is the rate-limiting enzyme of the desaturation of linoleic acid in theproduction of a human essential fatty acid, g-linolenic acid(GLA). The production of g-linolenic acid(GLA) was observedin soybean callus cells, which were transformed with this vector. This confirmed the activity of the activity fragmentBCSP666.展开更多
文摘Great uncertainty exists in reservoir models built for blocks where well spacing is uneven or large. The uncertainty in reservoir models can be significantly reduced by using Coordinate Cokriging Sequential Gaussian Simulation technology, in combination with the restriction of seismic characteristic data. Satisfactory reservoir parameter interpolation results, which are more accurate than those derived only from borehole data, are obtained, giving rise to a reasonable combination of widespread and dense-sampled seismic (soft data) data with borehole data (hard data). A significant effect has been made in reservoir parameter modeling in the Chegu 201 block of the Futai Oilfield by using this technology.
基金supported by the National Natural Science Foundation of China(30200176)the Key Foundation of Tianjin of China(013802511)
文摘The promoter region (BCSP666) of b-conglycinin a-subunit gene from the genomic DNA of soybean Jilin 43 was isolatedby PCR method. Sequencing analysis showed that the cloned fragment BCSP666 had the similar structure to the soybeanseed-specific promoter b-conglycinin a'-subunit gene promoter and b-conglycinin b-subunit gene promoter, and it alsocontains many motifs that contribute to the seed-specific promoter activity. Based on this sequencing analysis, wededuced that promoter fragment BCSP666 had the seed-sepecific promoter activity. And then we constructed the seed-specific expression vector pBMI666 with the promoter fragment BCSP666 and D6-fatty acid desaturase gene fromMortierella isabellina. The D6-fatty acid desaturase is the rate-limiting enzyme of the desaturation of linoleic acid in theproduction of a human essential fatty acid, g-linolenic acid(GLA). The production of g-linolenic acid(GLA) was observedin soybean callus cells, which were transformed with this vector. This confirmed the activity of the activity fragmentBCSP666.
