Resurrection plants, which are the ＂gifts＂ of natural evolution, are ideal models for studying the genetic basis of plant desiccation tolerance. Here, we report a high-quality genome assembly of 301 Mb for the diplo...Resurrection plants, which are the ＂gifts＂ of natural evolution, are ideal models for studying the genetic basis of plant desiccation tolerance. Here, we report a high-quality genome assembly of 301 Mb for the diploid spike moss Selaginella tamariscina, a primitive vascular resurrection plant. We predicated 27 761 protein-coding genes from the assembled S. tarnariscina genome, 11.38% （2363） of which showed signifi- cant expression changes in response to desiccation. Approximately 60.58% of the S. tamariscina genome was annotated as repetitive DNA, which is an almost 2-fold increase of that in the genome of desiccation- sensitive Selaginella moellendorffii. Genomic and transcriptomic analyses highlight the unique evolution and complex regulations of the desiccation response in S. tamariscina, including species-specific expan- sion of the oleosin and pentatricopeptide repeat gene families, unique genes and pathways for reactive oxygen species generation and scavenging, and enhanced abscisic acid （ABA） biosynthesis and potentially distinct regulation of ABA signaling and response. Comparative analysis of chloroplast genomes of several Selaginella species revealed a unique structural rearrangement and the complete loss of chloroplast NAD（P） H dehydrogenase （NDH） genes in S. tamariscina, suggesting a link between the absence of the NDH com- plex and desiccation tolerance. Taken together, our comparative genomic and transcriptomic analyses reveal common and species-specific desiccation tolerance strategies in S. tamariscina, providing signifi- cant insights into the desiccation tolerance mechanism and the evolution of resurrection plants.展开更多
A total of 21 compounds were isolated from the seeds of Capparis masaikai and identified as oxazolidine-2-thione(1), succinimide(2), catechol(3), octathiocane(4), monoethanolamine(5), 3-hydroxypropanenitrile(6), L-ara...A total of 21 compounds were isolated from the seeds of Capparis masaikai and identified as oxazolidine-2-thione(1), succinimide(2), catechol(3), octathiocane(4), monoethanolamine(5), 3-hydroxypropanenitrile(6), L-arabinose(7), 1,2,3-propanetriol(8), drummondol(9), spionoside B(10), adenosine(11), corchoionoside C(12), coniferin(13), syringin(14), cis-syringin(15), dihydrosyringin(16), indole-3-carboxylic acid(17), β-D-glucopyranosyl indole-3-carboxylic acid(18), 6-hydroxyindole-3-carboxylic acid β-D-glucopyranosyl ester(19), linoleis monoglyceride(20), and triolein(21). Their structures were identified based on physicochemical property and spectroscopic analysis, including MS, NMR, and single-crystal X-ray crystallographic data. Compounds 2–10 and 12–21 were isolated from C. masaikai for the first time.展开更多
文摘Resurrection plants, which are the ＂gifts＂ of natural evolution, are ideal models for studying the genetic basis of plant desiccation tolerance. Here, we report a high-quality genome assembly of 301 Mb for the diploid spike moss Selaginella tamariscina, a primitive vascular resurrection plant. We predicated 27 761 protein-coding genes from the assembled S. tarnariscina genome, 11.38% （2363） of which showed signifi- cant expression changes in response to desiccation. Approximately 60.58% of the S. tamariscina genome was annotated as repetitive DNA, which is an almost 2-fold increase of that in the genome of desiccation- sensitive Selaginella moellendorffii. Genomic and transcriptomic analyses highlight the unique evolution and complex regulations of the desiccation response in S. tamariscina, including species-specific expan- sion of the oleosin and pentatricopeptide repeat gene families, unique genes and pathways for reactive oxygen species generation and scavenging, and enhanced abscisic acid （ABA） biosynthesis and potentially distinct regulation of ABA signaling and response. Comparative analysis of chloroplast genomes of several Selaginella species revealed a unique structural rearrangement and the complete loss of chloroplast NAD（P） H dehydrogenase （NDH） genes in S. tamariscina, suggesting a link between the absence of the NDH com- plex and desiccation tolerance. Taken together, our comparative genomic and transcriptomic analyses reveal common and species-specific desiccation tolerance strategies in S. tamariscina, providing signifi- cant insights into the desiccation tolerance mechanism and the evolution of resurrection plants.
基金National Science and Technology Major Project(Major New Drug Development,Grant No.2016ZX09101069)。
文摘A total of 21 compounds were isolated from the seeds of Capparis masaikai and identified as oxazolidine-2-thione(1), succinimide(2), catechol(3), octathiocane(4), monoethanolamine(5), 3-hydroxypropanenitrile(6), L-arabinose(7), 1,2,3-propanetriol(8), drummondol(9), spionoside B(10), adenosine(11), corchoionoside C(12), coniferin(13), syringin(14), cis-syringin(15), dihydrosyringin(16), indole-3-carboxylic acid(17), β-D-glucopyranosyl indole-3-carboxylic acid(18), 6-hydroxyindole-3-carboxylic acid β-D-glucopyranosyl ester(19), linoleis monoglyceride(20), and triolein(21). Their structures were identified based on physicochemical property and spectroscopic analysis, including MS, NMR, and single-crystal X-ray crystallographic data. Compounds 2–10 and 12–21 were isolated from C. masaikai for the first time.
