LncRNAs unraveling their sponge role in glioblastoma and potential therapeutic applications

Glioblastoma multiforme(GBM),the most common and aggressive primary brain tumor in adults,is the most malignant and still has no cure.However,the novel role of long non-coding RNAs(lncRNAs)in the pathogenesis of glioblastoma is attracting extensive attention.LncRNAs are transcribed RNA molecules over 200 nucleotides long that do not encode proteins.Unlike small non-coding RNAs,such as microRNAs(miRNAs),lncRNAs have more complex secondary and tertiary structures that enable them to interact with DNA,RNA,and proteins and perform multiple regulatory functions.LncRNAs act as molecular sponges,absorbing and sequestering other biomolecules,particularly miRNAs,thereby preventing these molecules from performing their normal functions.LncRNAs influence glioblastoma through gene expression regulation,molecular sponge capacity,epigenetic modulation,and signaling pathway interactions.In glioblastoma,a large number of lncRNAs have been found to be abnormally expressed,affecting tumor growth,invasion and resistance to treatment.Due to its regulatory role and disease-specific expression patterns,lncRNA has become a potential biomarker for glioblastoma and a promising new therapeutic target.This paper discusses the spongy role of lncRNAs in glioblastoma and its potential therapeutic applications,which will lay a foundation for our understanding of glioblastoma biology and the development of new diagnostic and therapeutic strategies in the future.

Salen-Cu(Ⅱ)@MIL-101(Cr)as an efficient heterogeneous catalyst for cycloaddition of CO2 to epoxides under mild conditions

A double active center system, namely Salen-Cu（Ⅱ）@MIL-101（Cr）, was successfully synthesized via the"ship in a bottle" approach, which acted as a bifunctional material for both capture and conversion of COin a single process. For the first time, Salen-Cu（Ⅱ）@MIL-101（Cr） catalyst was developed for the synthesis of propylene carbonate from COand propylene oxide under room temperature and ambient pressure with a yield of 87.8% over 60 h. Furthermore, the reaction mechanism was also discussed.

Nanopore ultra-long sequencing and adaptive sampling spur plant complete telomere-totelomere genome assembly

The pursuit of complete telomere-to-telomere(T2T)genome assembly in plants,challenged by genomic complexity,has been advanced by Oxford Nanopore Technologies(ONT),which offers ultra-long,realtime sequencing.Despite its promise,sequencing length and gap filling remain significant challenges.This study optimized DNA extraction and library preparation,achieving DNA lengths exceeding 485 kb;average N50 read lengths of 80.57 kb,reaching up to 440 kb;and maximum reads of 5.83 Mb.Importantly,we demonstrated that combining ultra-long sequencing and adaptive sampling can effectively fill gaps during assembly,evidenced by successfully filling the remaining gaps of a near-complete Arabidopsis genome assembly and resolving the sequence of an unknown telomeric region in watermelon genome.Collectively,our strategies improve the feasibility of complete T2T genomic assemblies across various plant species,enhancing genome-based research in diverse fields.