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比较基因组学分析不同来源罗伊氏乳杆菌基因多样性及生境适应性 被引量:4
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作者 安晓娜 李伟程 +4 位作者 于洁 潘琳 莫蓝馨 姚彩青 张和平 《微生物学报》 CAS CSCD 北大核心 2020年第5期875-886,共12页
【目的】研究34株不同来源罗伊氏乳杆菌(Lactobacillus reuteri)基因多样性及生境适应性机制,了解该菌株在肠外生境及肠内生境中适应性的异同,为L.reuteri优良菌株的开发提供理论基础。【方法】基于二代测序平台对11株源于发酵食品(酸... 【目的】研究34株不同来源罗伊氏乳杆菌(Lactobacillus reuteri)基因多样性及生境适应性机制,了解该菌株在肠外生境及肠内生境中适应性的异同,为L.reuteri优良菌株的开发提供理论基础。【方法】基于二代测序平台对11株源于发酵食品(酸马奶、酸粥)的L.reuteri进行测序,并应用比较基因组学将其与发酵食品、酸面团、食草动物L.reuteri分离株基因组进行比较分析。【结果】分离自酸马奶、酸粥L.reuteri基因组大小平均为2.14 Mb,GC含量平均为38.77%,且同种来源分离株系统发育关系距离较近。泛-核心基因集分别包含7242个、969个基因家族,其中酸马奶分离株特异性基因最多为459个。功能注释结果显示不同来源菌株碳水化合物、氨基酸相关基因数量及种类差异较大,仅在发酵食品和食草动物株中发现抗生素耐药性基因。注释到的碳水化合物活性酶中仅出现在发酵食品与食草动物分离株的为GH3(β-葡萄糖苷酶等)和GH43(β-木糖苷酶等),特有的分别为AA3(纤维二糖脱氢酶等)和GH66(葡萄糖转移酶等)。【结论】不同来源L.reuteri具有广泛的基因多样性,与生存环境密切相关。发酵食品分离株具有部分食草动物肠道菌株特性且具有其独特的环境适应性特征,体现了与宿主有关的环境适应能力,可加深对食品发酵和肠内环境中细菌生境适应性的理解。 展开更多
关键词 罗伊氏乳杆菌 比较基因组学 发酵食品 生境适应性
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Changes in chemical composition,structural and functional microbiome during alfalfa(Medicago sativa)ensilage with Lactobacillus plantarum PS-8 被引量:5
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作者 Lijun You Weichen Bao +6 位作者 caiqing yao Feiyan Zhao Hao Jin Weiqiang Huang Bohai Li Lai-Yu Kwok Wenjun Liu 《Animal Nutrition》 SCIE CSCD 2022年第2期100-109,共10页
Improving silage production by adding exogenous microorganisms not only maximizes nutrient preservation,but also extends product shelf life.Herein,changes in the quality and quantity of Lactobacillus plantarum PS-8(PS... Improving silage production by adding exogenous microorganisms not only maximizes nutrient preservation,but also extends product shelf life.Herein,changes in the quality and quantity of Lactobacillus plantarum PS-8(PS-8)-inoculated alfalfa(Medicago sativa)during silage fermentation were monitored at d 0,7,14,and 28(inoculum dose of PS-8 was 1105 colony forming units[cfu]/g fresh weight;50 kg per bag;10 bags for each time point)by reconstructing metagenomic-assembled genomes(MAG)and Growth Rate InDex(GRiD).Our results showed that the exogenous starter bacterium,PS-8 inoculation,became the most dominating strain by d 7,and possibly played a highly active role throughout the fermentation process.The pH value of the silage decreased greatly,accompanied by the growth of acidproducing microorganisms namely PS-8,which inhibited the growth of harmful microorganisms like molds(4.18 vs.1.42 log cfu/g)and coliforms(4.95 vs.0.66 log most probable number[MPN]/g).The content of neutral detergent fiber(NDF)decreased significantly(41.6%vs.37.6%;dry matter basis).In addition,the abundance and diversity of genes coding microbial carbohydrate-active enzymes(CAZymes)increased significantly and desirably throughout the fermentation,particularly the genes responsible for degrading starch,arabino-xylan,and cellulose.Overall,our results showed that PS-8 was replicating rapidly and consistently during early-and mid-fermentation phases,promoting the growth of beneficial lactic acid bacteria and inhibiting undesirable microbes,ultimately improving the quality of silage. 展开更多
关键词 Lactobacillus plantarum PS-8 Alfalfa silage Lactic acid bacteria Microbiota community Metagenomic-assembled genome
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