To investigate the mechanical properties of ice-saturated frozen soil, a series of triaxial tests under various confining pressures（0.5 to 9.0 MPa） on ice-saturated frozen loess with ice content of 23.7% were carrie...To investigate the mechanical properties of ice-saturated frozen soil, a series of triaxial tests under various confining pressures（0.5 to 9.0 MPa） on ice-saturated frozen loess with ice content of 23.7% were carried out at a temperature-6 °C, and at 1.25 mm/min of loading rate. The triaxial tests include two loading modes, one with monotonic loading（i.e., triaxial compression）, and another with static cycle loading. The test results under triaxial compression show that the strength and deformation behaviors of ice-saturated frozen loess are affected by confining pressure. According to the test results of triaxial loading-unloading cycle test, the elastic modulus evolution with the number of cycles under different confining pressures are analyzed.展开更多
Traumatic spinal cord injury(SCI) is a major challenge in the clinic. In this study, we sought to examine the synergistic effects of linear ordered collagen scaffold(LOCS) and human placenta-derived mesenchymal stem c...Traumatic spinal cord injury(SCI) is a major challenge in the clinic. In this study, we sought to examine the synergistic effects of linear ordered collagen scaffold(LOCS) and human placenta-derived mesenchymal stem cells(hPMSCs) when transplanted into completely transected beagle dogs. After 36 weeks observation, we found that LOCS+hPMSCs implants promoted better hindlimb locomotor recovery than was observed in the non-treatment(control) group and LOCS group. Histological analysis showed that the regenerated tissue after treatment was well integrated with the host tissue, and dramatically reduced the volume of cystic and chondroitin sulfate proteoglycans(CSPGs) expression. Furthermore, the LOCS+hPMSCs group also showed more neuron-specific βIII-tubulin(Tuj-1)-and NeuN-positive neurons in the lesion area, as well as axonal regeneration, remyelination and synapse formation in the lesion site. Additionally, dogs in the LOCS+hPMSCs group experienced enhanced sprouting of both ascending(CGRP-positive) sensory fibers and descending(5-HT-and TH-positive) motor fibers at the lesion area. All these data together suggested that the combined treatment had beneficial effects on neuronal regeneration and functional improvement in a canine complete transection model. Therefore, LOCS+hPMSCs implantation holds a great promise for bridging the nerve defect and may be clinically useful in the near future.展开更多
基金supported by the National Natural Science Foundation of China (41301072, 41230630,41101068)National Key Basic Research Program of China (973 Program No. 2012CB026102)+2 种基金Natural Science Foundation of Inner Mongolia (2013MS0702)the Program of Higher-level talents of Inner Mongolia University (30105-125146)the Open Project Program of the State Key Laboratory of Frozen Soil Engineering (SKLFSE201208)
文摘To investigate the mechanical properties of ice-saturated frozen soil, a series of triaxial tests under various confining pressures（0.5 to 9.0 MPa） on ice-saturated frozen loess with ice content of 23.7% were carried out at a temperature-6 °C, and at 1.25 mm/min of loading rate. The triaxial tests include two loading modes, one with monotonic loading（i.e., triaxial compression）, and another with static cycle loading. The test results under triaxial compression show that the strength and deformation behaviors of ice-saturated frozen loess are affected by confining pressure. According to the test results of triaxial loading-unloading cycle test, the elastic modulus evolution with the number of cycles under different confining pressures are analyzed.
基金supported by the "Strategic Priority Research Program of the Chinese Academy of Sciences" (XDA01030000)the key Research Program of the Chinese Academy of Sciences (ZDRW-ZS-2016-2)+3 种基金the National Natural Science Foundation of China (81572131, 81571213)the Natural Science Foundation of Jiangsu Province (BL2012004, BK20151210)the Priority Academic Program Development of Jiangsu Higher Education Institutionsthe key Research and Development Program of Ministry of Science and Technology (2016YFC1101500)
文摘Traumatic spinal cord injury(SCI) is a major challenge in the clinic. In this study, we sought to examine the synergistic effects of linear ordered collagen scaffold(LOCS) and human placenta-derived mesenchymal stem cells(hPMSCs) when transplanted into completely transected beagle dogs. After 36 weeks observation, we found that LOCS+hPMSCs implants promoted better hindlimb locomotor recovery than was observed in the non-treatment(control) group and LOCS group. Histological analysis showed that the regenerated tissue after treatment was well integrated with the host tissue, and dramatically reduced the volume of cystic and chondroitin sulfate proteoglycans(CSPGs) expression. Furthermore, the LOCS+hPMSCs group also showed more neuron-specific βIII-tubulin(Tuj-1)-and NeuN-positive neurons in the lesion area, as well as axonal regeneration, remyelination and synapse formation in the lesion site. Additionally, dogs in the LOCS+hPMSCs group experienced enhanced sprouting of both ascending(CGRP-positive) sensory fibers and descending(5-HT-and TH-positive) motor fibers at the lesion area. All these data together suggested that the combined treatment had beneficial effects on neuronal regeneration and functional improvement in a canine complete transection model. Therefore, LOCS+hPMSCs implantation holds a great promise for bridging the nerve defect and may be clinically useful in the near future.
