Herbal extraction residues(HERs)cause serious environmental pollution and resource waste.In this study,a novel green route was designed for the comprehensive reutilization of all components in HERs,taking Magnolia off...Herbal extraction residues(HERs)cause serious environmental pollution and resource waste.In this study,a novel green route was designed for the comprehensive reutilization of all components in HERs,taking Magnolia officinalis residues(MOR)as an example.The reluctant structure of MOR was first destroyed by alkali pretreatment to release the functional ingredients(magnolol and honokiol)originally remaining in MOR and to make MOR more accessible for hydrolysis.A metal–organic frame material MIL-101(Cr)with a maximum absorption capacity of 255.64 mg g^(-1)was synthesized to absorb the released honokiol and magnolol from the pretreated MOR solutions,and 40 g L^(-1)reducing sugars were obtained with 81.8%enzymatic hydrolysis rate at 10%MOR solid loading.Finally,382 mg L-1β-amyrin was produced from MOR hydrolysates by an engineered yeast strain.In total,1 kg honokiol,8 kg magnolol,and 7.64 kg β-amyrin could produce from 1 ton MOR by this cleaner process with a total economic output of 170,700 RMB.展开更多
The rapid spread of viral zoonoses can cause severe consequences,including huge economic loss,public health problems or even global crisis of society.Clinical detection technology plays a very important role in the pr...The rapid spread of viral zoonoses can cause severe consequences,including huge economic loss,public health problems or even global crisis of society.Clinical detection technology plays a very important role in the prevention and control of such zoonoses.The rapid and accurate detection of the pathogens of the diseases can directly lead to the early report and early successful control of the diseases.With the advantages of being easy to use,fast,portable,multiplexing and cost-effective,semiconductor biosensors are kinds of detection devices that play an important role in preventing epidemics,and thus have become one of the research hotspots.Here,we summarized the advances of semiconductor biosensors in viral zoonoses detection.By discussing the major principles and applications of each method for different pathogens,this review proposed the directions of designing semiconductor biosensors for clinical application and put forward perspectives in diagnostic of viral zoonoses.展开更多
Azadirachtin,a complex tetratriterpenoid limonin with potent insecticidal properties,is the most widely used biological pesticide worldwide.Its versatile pharmacological applications include the inhibition of tumor gr...Azadirachtin,a complex tetratriterpenoid limonin with potent insecticidal properties,is the most widely used biological pesticide worldwide.Its versatile pharmacological applications include the inhibition of tumor growth and anti-malarial,anti-bacterial,and anti-inflammatory properties.Azadirachtin plays a pivotal role in pest control and novel drug development.The primary source of azadirachtin is the neem tree(Azadirachta indica A.Juss),with an azadirachtin content ranging from 0.3%to 0.5%.Despite the market demand for botanical pesticides reaching approximately 100,000 tons per year,the annual neem production in China is only 1.14 tons.Although azadirachtin can be obtained through plant extraction or chemical synthesis,the quantity obtained does not meet the market demand in China.The sluggish pace of azadirachtin biosynthesis results from the limited availability of genetic information and the complexity of the synthetic pathway.Recent advancements in azadirachtin biosynthesis hold promise as an efficient collection method.In this study,we explored the physicochemical properties,biological activities,mechanisms of action,and acquisition methods of azadirachtin.We also delved into recent progress in azadirachtin biosynthesis and assessed potential future usage challenges.This study aims to establish a theoretical foundation for the scientific application and efficient synthesis of azadirachtin,offering valuable reference information to the industry.展开更多
The productivity and health of seagrass depend on the combined inputs of nutrients from the water and sediments in which they grow and the microbiota with which they live intimately.However,little is known about the c...The productivity and health of seagrass depend on the combined inputs of nutrients from the water and sediments in which they grow and the microbiota with which they live intimately.However,little is known about the composition and diversity pattern of single-celled benthic eukaryotes in seagrass meadows.Here,we investigated how the structure and diversity of the benthic microeukaryotic community vary with respect to season,location,and seagrass colonization,by applying 18S rRNA gene amplicon sequencing for 96 surface sediment samples that were collected from three different seagrass habitats through four seasons.We found that benthic microeukaryotic communities associated with seagrass Zostera japonica exhibited remarkable spatial and seasonal variations,as well as differences between vegetated and unvegetated sediments.Diatoms and dinoflagellates predominated in the benthic microeukaryotic communities,but they were inversely correlated and displaced each other as the dominant microbial group in different seasons or habitats.Mucoromycota was more prevalent in vegetated sediments,whereas Lobulomycetales and Chytridiales had higher proportions in unvegetated sites.Total organic carbon and total organic nitrogen were the most important environmental factors in driving the microeukaryotic assemblages and diversity.Our study expands the available knowledge on the biogeographic distribution patterns and niche preferences for benthic microeukaryotes in seagrass systems.展开更多
Harmful algal bloom(HAB)is an ecological disaster to local mariculture.At present,its impact on macrophytes has not been well studied.In this study,we cultivated sexually propagated embryos of S argassum fusiformis-an...Harmful algal bloom(HAB)is an ecological disaster to local mariculture.At present,its impact on macrophytes has not been well studied.In this study,we cultivated sexually propagated embryos of S argassum fusiformis-an edible seaweed-in Prorocentrum donghaiense suspensions at different cell densities(0,0.50×10^(5),0.75×10^(5),1.00×10^(5),and 1.50×10^(5) cells/mL)for 10 days,during which growth and photosynthetic activities of the embryos were determined,and a monocultivation was set up for comparison.Results show that the relative growth rate and photosynthetic activities of the embryos co-cultivated with P.donghaiense were inhibited mostly and significantly in the cell densities of 0.75×10^(5),1.00×10^(5),and 1.50×10^(5) cells/mL,and the inhibitory effects increased in overall with increased cell densities.The maximum relative electron transport rates(rETR max)and apparent photosynthetic efficiency(a)of co-cultivated embryos were all significantly lower than monocultivation ones on the 10 th day.Furthermore,the photosynthetic activity detected by chlorophyll-a fluorescence transient(i.e.,OJIP),the electron transport among electron transfer accepters of PSII(photosystem II)and that from PSII to PSI(photosystem I)was restricted,which is probably responsible for the decreases of rETR max andain the co-cultivated embryos.In addition,parts of the photosynthetic reaction centers of PSII in the co-cultivated embryos were inactivated.Therefore,P.donghaiense bloom could restrain the development and photosynthetic activities of S.fusiformis embryos,reduce the seedlings stock,and eventually hinder the development of S.fusiformis production industry.展开更多
Uncovering the role of environmental factors and finding critical factors which harbor significant fractions in governing microbial communities remain key questions in coastal marine systems.To detect the interactions...Uncovering the role of environmental factors and finding critical factors which harbor significant fractions in governing microbial communities remain key questions in coastal marine systems.To detect the interactions between environmental factors and distributions of virio-and bacterioplankton in trophic coastal areas,we used flow cytometry to investigate the abundance of virio-and bacterioplankton covering 31 stations in the Bohai Sea of China.Our results suggested that the average abundance of total virus(TV)in winter(~2.29×108 particles/mL)was slightly lower than in summer(~3.83×108 particles/mL).The mean total bacterial abundance(TB)was much lower in winter(~2.54×107 particles/mL)than in summer(~5.43×107 particles/mL).Correlation analysis via redundancy analysis(RDA)and network analysis among virioplankton,bacterioplankton and environmental factors revealed that the abundances of viral and bacterial subpopulations depend on environmental factors.In winter,only temperature significantly influenced the abundances of virio-and bacterioplankton.In summer,in addition to temperature,both salinity and nutrient(SiO2)had a remarkable impact on the distribution of virioand bacterioplankton.Our results showed a clear seasonal and trophic pattern throughout the whole water system,which revealed that temperature and eutrophication may play crucial roles in microbial distribution pattern.展开更多
Currently,people pay more and more attention to the transitional resettlement of victims after various natural disasters.There is an urgent need for a large number of temporary houses to resettle the victims after nat...Currently,people pay more and more attention to the transitional resettlement of victims after various natural disasters.There is an urgent need for a large number of temporary houses to resettle the victims after natural disasters.Disaster-relief temporary houses(DTHs)played an important role in the post-disaster resettlement in the past,which can not only be produced on a large scale,but also can be quickly and conveniently erected,which were the main means to solve the problem of transitional resettlement.However,due to their temporary nature,there was no extra energy consuming system installed in the DTHs generally.Hence the indoor thermal environment inside the DTHs was severe in summer.In this study,combined with the field experimental tests of the DTHs in Wenchuan Earthquake and Lushan Earthquake and the experimental study of the full-size DTH,it found that the thermal environment inside the DTH was intolerably high in summer.It had negative impact on victims.In order to improve the thermal environment inside DTHs during post-disaster period which lacked of extra energy resources,this study used the method of combining phase change materials(PCMs)with walls of the DTH to explore its feasibility and effectiveness.The results showed that PCMs could effectively improve the thermal environment inside the DTH in summer.Furthermore,the difference of the composite positions between PCMs and the wall affected the improvement effect.The energy release rate of the PCMs assembly system(PAS)varied according to the positions of the PCMs.展开更多
Increasing evidence supports the hypothesis of autologous immune attack in severe aplastic anemia(SAA):the predominant role of activated cytotoxic T cells(CTL)expressing-interferon in inhibiting the growth of bone mar...Increasing evidence supports the hypothesis of autologous immune attack in severe aplastic anemia(SAA):the predominant role of activated cytotoxic T cells(CTL)expressing-interferon in inhibiting the growth of bone marrow(BM)cells,putative autoantigens,and oligoclonal expansion of CD8+T cells.1 For SAA patients,the definitive therapies are immunosuppressive therapy(IST)or hematopoietic stem transplantation(HSCT).展开更多
Glioblastoma(GBM)is the most common and aggressive malignant brain tumor in adults and is poorly controlled.Previous studies have shown that both macrophages and angiogenesis play significant roles in GBM progression,...Glioblastoma(GBM)is the most common and aggressive malignant brain tumor in adults and is poorly controlled.Previous studies have shown that both macrophages and angiogenesis play significant roles in GBM progression,and co-targeting of CSF1R and VEGFR is likely to be an effective strategy for GBM treatment.Therefore,this study developed a novel and selective inhibitor of CSFIR and VEGFR,SYHA1813,possessing potent antitumor activity against GBM.SYHA1813 inhibited VEGFR and CSFIR kinase activities with high potency and selectivity and thus blocked the cell viability of HUVECs and macrophages and exhibited anti-angiogenetic effects both in vitro and in vivo.SYHA1813 also displayed potent in vivo antitumor activity against GBM in immune-competent and immune-deficient mouse models,including temozolomide(TMZ)insensitive tumors.Notably,SYHA1813 could penetrate the blood-brain barrier(BBB)and prolong the survival time of mice bearing intracranial GBM xenografts.Moreover,SYHA1813 treatment resulted in a synergistic antitumor efficacy in combination with the PD-1 antibody.As a clinical proof of concept,SYHA1813 achieved confirmed responses in patients with recurrent GBM in an ongoing first-in-human phase I trial.The data of this study support the rationale for an ongoing phase I clinical study(ChiCTR2100045380).展开更多
Quasi-2D layered Cr4Te5 thin film has attracted great attention because it possesses the high Curie temperature close to room temperature and relatively large saturation magnetization.However,the magnetic interactions...Quasi-2D layered Cr4Te5 thin film has attracted great attention because it possesses the high Curie temperature close to room temperature and relatively large saturation magnetization.However,the magnetic interactions and the nature of magnetic phase transition in the Cr4Te5 film have not been explored thoroughly.In this paper,we focused on the critical behavior of its magnetic phase transition through the epitaxial Cr4Te5 film fabricated by pulsed laser deposition(PLD).The final critical exponentsβ=0.359(2)andγ=1.54(2)were obtained by linear extrapolation together with ArrottNoakes equation of state,and their accuracy was confirmed by using the Widom scaling relation and scaling hypothesis.We find that some magnetic disorders exist in the Cr4Te5 film system,which is related to Cr4Te5 critical behavior why its critical behavior is quite far from any conventional universality class.Furthermore,we also determined that the Cr4Te5 film exhibits a quasi-2D long-range magnetic interaction.Finally,the itinerant ferromagnets of Cr4Te5 films were confirmed by the Takahashi’s self-consistent renormalization theory of spin fluctuations.Our work provides a new idea for understanding the mechanism of magnetic interactions in similar 2D layered films.展开更多
The noise reduction effect of noise barriers has been extensively studied,but the effect on pollutant dispersion remains unclear.A computational fluid dynamics(CFD)simulation is conducted to investigate the effects of...The noise reduction effect of noise barriers has been extensively studied,but the effect on pollutant dispersion remains unclear.A computational fluid dynamics(CFD)simulation is conducted to investigate the effects of different heights,lengths,and types of noise barriers and different wind speeds on pollutant dispersion in street canyons with viaducts.The field synergy theory of the convective mass transfer process is used for quantitative analysis of pollutant dispersion in street canyons.The results show that as the height and length of the noise barrier increase,the pollutant dispersion capacity decreases.As the wind speed increases,the rate of decrease in the average CO concentration declines.The effect of the wind speed on the synergistic improvement of the speed and concentration gradient vectors differs for different types of noise barriers.The performance follows the order:fully-closed noise barrier>left noise barrier>right noise barrier>semi-closed noise barrier.The different noise barrier types significantly impact the flow field and pollutant dispersion and reduce the CO concentration to varying degrees,except for the fully-closed type.The average CO concentration in the pedestrian breathing zone is reduced by a maximum of 55.85%on the leeward side and by 53%on the windward side,indicating that an appropriate noise barrier on the viaduct reduces noise pollution and improves the air quality in street canyons,especially in the pedestrian breathing zone.展开更多
Background:Poor sleep quality is associated with a decrease in quality of life in patients with major burn scars,combined with pruritus and pain.Few interventions have been reported to improve the sleep quality of pat...Background:Poor sleep quality is associated with a decrease in quality of life in patients with major burn scars,combined with pruritus and pain.Few interventions have been reported to improve the sleep quality of patients with scars.In the current prospective cohort study,we investigated the efficacy of CO_(2)-ablative fractional laser(AFL)surgery vs conventional surgery in post-burn patients with hypertrophic scars with sleep quality as the primary study outcome.Methods:In total 68 consecutive patients undergoing scar surgical treatment were recruited,including a CO_(2)-AFL surgery cohort(n=35)and a conventional surgery cohort(n=33).A subgroup from the AFL cohort was selected.Sleep quality,pain and pruritus were evaluated.Multiple linear regression analyses were performed to reveal the effect of CO_(2)-AFL surgery.Results:The CO_(2)-AFL surgery cohort had significantly lower Pittsburgh sleep quality index(PSQI)global scores than the conventional surgery cohort after the last surgical treatment.In the subgroup of patients receiving hardware sleep monitoring,CO_(2)-AFL markedly increased deep sleep time,deep sleep efficiency and reduced initial sleep latency.Compared to the conventional surgery cohort,the CO_(2)-AFL cohort presented significantly lower pain and pruritus scores.Correlation analysis showed pain and pruritus were significantly associated with PSQI scores,and there were also significant correlations between pain and pruritus scores.Multiple linear regression analysis showed that surgery method was negatively linearly correlated with visual analog scale(VAS)pain score,brief pain inventory(BPI)total,VAS pruritus score,5-D itch scale total,four-item itch questionnaire(FIIQ)total and PSQI total.Conclusions:CO_(2)-AFL surgery significantly improved sleep quality and reduced pain and pruritus of hypertrophic scar patients.The alleviation of sleep disorder was associated with improvement of deep sleep quality including deep sleep time and deep sleep deficiency.Trial registration:The Chinese Clinical Trial Registry(ChiCTR200035268)approved retrospectively registration on 5 Aug 2020.展开更多
In this paper, anisotropic Crouzeix-Raviart type nonconforming finite element meth- ods are considered for solving the second order variational inequality with displacement obstacle. The convergence analysis is presen...In this paper, anisotropic Crouzeix-Raviart type nonconforming finite element meth- ods are considered for solving the second order variational inequality with displacement obstacle. The convergence analysis is presented and the optimal order error estimates are obtained under the hypothesis of the finite length of the free boundary. Numerical results are provided to illustrate the correctness of theoretical analysis.展开更多
Tyrosine-decahydrofluorene derivatives feature a fused[6.5.6]tricarbocyclic core and a 13-membered para-cyclophane ether.Herein,we identified new xenoacremones A,B,and C(1-3)from the fungal strain Xenoacremonium sinen...Tyrosine-decahydrofluorene derivatives feature a fused[6.5.6]tricarbocyclic core and a 13-membered para-cyclophane ether.Herein,we identified new xenoacremones A,B,and C(1-3)from the fungal strain Xenoacremonium sinensis ML-31 and elucidated their biosynthetic pathway using gene deletion in the native strain and heterologous expression in Aspergillus nidulans.The hybrid polyketide synthaseenonribosomal peptide synthetase(PKS-NRPS)XenE together with enoyl reductase XenG were confirmed to be responsible for the formation of the tyrosine-nonaketide skeleton.This skeleton was subsequently dehydrated by XenA to afford a pyrrolidinone moiety.XenF catalyzed a novel sigmatropic rearrangement to yield a key cyclohexane intermediate as a prerequisite for the formation of the multi-ring system.Subsequent oxidation catalyzed by XenD supplied the substrate for XenC to link the para-cyclophane ether,which underwent subsequent spontaneous Diels-Alder reaction to give the end products.Thus,the results indicated that three novel enzymes XenF,XenD,and XenC coordinate to assemble the[6.5.6]tricarbocyclic ring and para-cyclophane ether during biosynthesis of complex tyrosine-decahydrofluorene derivatives.展开更多
基金supported by the National Key Research and Development Project(2019YFC1906601)China the Scientific and Technological Innovation Project of the Chinese Academy of Chinese Medical Sciences(C12021A04111)the Fundamental Research Funds for the Central Public Welfare Research Institutes(ZZ13-YQ-040).
文摘Herbal extraction residues(HERs)cause serious environmental pollution and resource waste.In this study,a novel green route was designed for the comprehensive reutilization of all components in HERs,taking Magnolia officinalis residues(MOR)as an example.The reluctant structure of MOR was first destroyed by alkali pretreatment to release the functional ingredients(magnolol and honokiol)originally remaining in MOR and to make MOR more accessible for hydrolysis.A metal–organic frame material MIL-101(Cr)with a maximum absorption capacity of 255.64 mg g^(-1)was synthesized to absorb the released honokiol and magnolol from the pretreated MOR solutions,and 40 g L^(-1)reducing sugars were obtained with 81.8%enzymatic hydrolysis rate at 10%MOR solid loading.Finally,382 mg L-1β-amyrin was produced from MOR hydrolysates by an engineered yeast strain.In total,1 kg honokiol,8 kg magnolol,and 7.64 kg β-amyrin could produce from 1 ton MOR by this cleaner process with a total economic output of 170,700 RMB.
基金supported by the National Key Research and Development Program of China(2022YFC2602100)supported by National key research and development program(2021YFC2600602)。
文摘The rapid spread of viral zoonoses can cause severe consequences,including huge economic loss,public health problems or even global crisis of society.Clinical detection technology plays a very important role in the prevention and control of such zoonoses.The rapid and accurate detection of the pathogens of the diseases can directly lead to the early report and early successful control of the diseases.With the advantages of being easy to use,fast,portable,multiplexing and cost-effective,semiconductor biosensors are kinds of detection devices that play an important role in preventing epidemics,and thus have become one of the research hotspots.Here,we summarized the advances of semiconductor biosensors in viral zoonoses detection.By discussing the major principles and applications of each method for different pathogens,this review proposed the directions of designing semiconductor biosensors for clinical application and put forward perspectives in diagnostic of viral zoonoses.
基金supported by the Scientific and Technological Innovation Project of the Chinese Academy of Chinese Medical Sciences (C12021A04111 and C12021A04116)the Fundamental Research Funds for the Central Public Welfare Research Institutes (ZZ14-YQ-031 and ZZ13-YQ-040)+1 种基金the National Key Research and Development Project (2019YFC19066)the National Natural Science Foundation of China (32200308).
文摘Azadirachtin,a complex tetratriterpenoid limonin with potent insecticidal properties,is the most widely used biological pesticide worldwide.Its versatile pharmacological applications include the inhibition of tumor growth and anti-malarial,anti-bacterial,and anti-inflammatory properties.Azadirachtin plays a pivotal role in pest control and novel drug development.The primary source of azadirachtin is the neem tree(Azadirachta indica A.Juss),with an azadirachtin content ranging from 0.3%to 0.5%.Despite the market demand for botanical pesticides reaching approximately 100,000 tons per year,the annual neem production in China is only 1.14 tons.Although azadirachtin can be obtained through plant extraction or chemical synthesis,the quantity obtained does not meet the market demand in China.The sluggish pace of azadirachtin biosynthesis results from the limited availability of genetic information and the complexity of the synthetic pathway.Recent advancements in azadirachtin biosynthesis hold promise as an efficient collection method.In this study,we explored the physicochemical properties,biological activities,mechanisms of action,and acquisition methods of azadirachtin.We also delved into recent progress in azadirachtin biosynthesis and assessed potential future usage challenges.This study aims to establish a theoretical foundation for the scientific application and efficient synthesis of azadirachtin,offering valuable reference information to the industry.
基金The National Key Research and Development Program under contract No.2020YFD0901003the National Natural Science Foundation of China under contract Nos 92051119,42077305 and 32070112+2 种基金the Special National Project on Investigation of Basic Resources of China under contract No.2019FY100700the Key Research Project of Frontier Science of Chinese Academy of Sciences under contract No.QYZDB-SSW-DQC041the Taishan Scholar Project Special Funding under contract No.Tspd20210317。
文摘The productivity and health of seagrass depend on the combined inputs of nutrients from the water and sediments in which they grow and the microbiota with which they live intimately.However,little is known about the composition and diversity pattern of single-celled benthic eukaryotes in seagrass meadows.Here,we investigated how the structure and diversity of the benthic microeukaryotic community vary with respect to season,location,and seagrass colonization,by applying 18S rRNA gene amplicon sequencing for 96 surface sediment samples that were collected from three different seagrass habitats through four seasons.We found that benthic microeukaryotic communities associated with seagrass Zostera japonica exhibited remarkable spatial and seasonal variations,as well as differences between vegetated and unvegetated sediments.Diatoms and dinoflagellates predominated in the benthic microeukaryotic communities,but they were inversely correlated and displaced each other as the dominant microbial group in different seasons or habitats.Mucoromycota was more prevalent in vegetated sediments,whereas Lobulomycetales and Chytridiales had higher proportions in unvegetated sites.Total organic carbon and total organic nitrogen were the most important environmental factors in driving the microeukaryotic assemblages and diversity.Our study expands the available knowledge on the biogeographic distribution patterns and niche preferences for benthic microeukaryotes in seagrass systems.
基金Supported by the National Key R&D Program of China(No.2018YFD0901500)the National Natural Science Foundation(Nos.41876124,61871293,41706147,42007372)。
文摘Harmful algal bloom(HAB)is an ecological disaster to local mariculture.At present,its impact on macrophytes has not been well studied.In this study,we cultivated sexually propagated embryos of S argassum fusiformis-an edible seaweed-in Prorocentrum donghaiense suspensions at different cell densities(0,0.50×10^(5),0.75×10^(5),1.00×10^(5),and 1.50×10^(5) cells/mL)for 10 days,during which growth and photosynthetic activities of the embryos were determined,and a monocultivation was set up for comparison.Results show that the relative growth rate and photosynthetic activities of the embryos co-cultivated with P.donghaiense were inhibited mostly and significantly in the cell densities of 0.75×10^(5),1.00×10^(5),and 1.50×10^(5) cells/mL,and the inhibitory effects increased in overall with increased cell densities.The maximum relative electron transport rates(rETR max)and apparent photosynthetic efficiency(a)of co-cultivated embryos were all significantly lower than monocultivation ones on the 10 th day.Furthermore,the photosynthetic activity detected by chlorophyll-a fluorescence transient(i.e.,OJIP),the electron transport among electron transfer accepters of PSII(photosystem II)and that from PSII to PSI(photosystem I)was restricted,which is probably responsible for the decreases of rETR max andain the co-cultivated embryos.In addition,parts of the photosynthetic reaction centers of PSII in the co-cultivated embryos were inactivated.Therefore,P.donghaiense bloom could restrain the development and photosynthetic activities of S.fusiformis embryos,reduce the seedlings stock,and eventually hinder the development of S.fusiformis production industry.
基金The National Basic Research Program(973 Program)of China under contract No.2015CB453300the Strategic Priority Research Programme of Chinese Academic of Sciences under contract No.XDA11020403+2 种基金the Key Research Project of Frontier Science of Chinese Academy of Sciences under contract No.QYZDB-SSW-DQC041the Technology Service Network Plan of Chinese Academy of Sciences under contract No.STS,ZSYS-006the External Cooperation Program of Chinese Academy of Sciences(supported by the Bureau of International Cooperation,the Chinese Academy of Sciences)under contract No.133337KYSB20180015。
文摘Uncovering the role of environmental factors and finding critical factors which harbor significant fractions in governing microbial communities remain key questions in coastal marine systems.To detect the interactions between environmental factors and distributions of virio-and bacterioplankton in trophic coastal areas,we used flow cytometry to investigate the abundance of virio-and bacterioplankton covering 31 stations in the Bohai Sea of China.Our results suggested that the average abundance of total virus(TV)in winter(~2.29×108 particles/mL)was slightly lower than in summer(~3.83×108 particles/mL).The mean total bacterial abundance(TB)was much lower in winter(~2.54×107 particles/mL)than in summer(~5.43×107 particles/mL).Correlation analysis via redundancy analysis(RDA)and network analysis among virioplankton,bacterioplankton and environmental factors revealed that the abundances of viral and bacterial subpopulations depend on environmental factors.In winter,only temperature significantly influenced the abundances of virio-and bacterioplankton.In summer,in addition to temperature,both salinity and nutrient(SiO2)had a remarkable impact on the distribution of virioand bacterioplankton.Our results showed a clear seasonal and trophic pattern throughout the whole water system,which revealed that temperature and eutrophication may play crucial roles in microbial distribution pattern.
文摘Currently,people pay more and more attention to the transitional resettlement of victims after various natural disasters.There is an urgent need for a large number of temporary houses to resettle the victims after natural disasters.Disaster-relief temporary houses(DTHs)played an important role in the post-disaster resettlement in the past,which can not only be produced on a large scale,but also can be quickly and conveniently erected,which were the main means to solve the problem of transitional resettlement.However,due to their temporary nature,there was no extra energy consuming system installed in the DTHs generally.Hence the indoor thermal environment inside the DTHs was severe in summer.In this study,combined with the field experimental tests of the DTHs in Wenchuan Earthquake and Lushan Earthquake and the experimental study of the full-size DTH,it found that the thermal environment inside the DTH was intolerably high in summer.It had negative impact on victims.In order to improve the thermal environment inside DTHs during post-disaster period which lacked of extra energy resources,this study used the method of combining phase change materials(PCMs)with walls of the DTH to explore its feasibility and effectiveness.The results showed that PCMs could effectively improve the thermal environment inside the DTH in summer.Furthermore,the difference of the composite positions between PCMs and the wall affected the improvement effect.The energy release rate of the PCMs assembly system(PAS)varied according to the positions of the PCMs.
基金supported by grants from the Innovative Clinical Technique of Guangzhou,China(No.2019GX04 and 2023C-GX01 to YPZ and SQW,respectively)2019 Annual Research Project of The China Marrow Donor Program(No.CMDP201902 to SQW)+1 种基金Guangzhou Municipal Science and Technology project(China)(No.202002030035 to SQW)the Natural Science Foundation of Guangdong Province,China(No.2018A0303130179 to MZ).
文摘Increasing evidence supports the hypothesis of autologous immune attack in severe aplastic anemia(SAA):the predominant role of activated cytotoxic T cells(CTL)expressing-interferon in inhibiting the growth of bone marrow(BM)cells,putative autoantigens,and oligoclonal expansion of CD8+T cells.1 For SAA patients,the definitive therapies are immunosuppressive therapy(IST)or hematopoietic stem transplantation(HSCT).
基金supported by grants from the Natural Science Foundation of China for Innovation Research Group(81821005)the National Natural Science Foundation of China(82273948 and 81573271)+2 种基金the"Personalized Medicines,Molecular Signaturebased Drug Discovery and Development",Strategic Priority Research Program of the Chinese Academy of Sciences(XDA12020203 and XDA12020228,China)the National Science&Technology Major Project"Key New Drug Creation and Manufacturing Program",China(2018ZX09711002-011-016)the Youth Innovation Promotion Association of CAS(2018324,China).
文摘Glioblastoma(GBM)is the most common and aggressive malignant brain tumor in adults and is poorly controlled.Previous studies have shown that both macrophages and angiogenesis play significant roles in GBM progression,and co-targeting of CSF1R and VEGFR is likely to be an effective strategy for GBM treatment.Therefore,this study developed a novel and selective inhibitor of CSFIR and VEGFR,SYHA1813,possessing potent antitumor activity against GBM.SYHA1813 inhibited VEGFR and CSFIR kinase activities with high potency and selectivity and thus blocked the cell viability of HUVECs and macrophages and exhibited anti-angiogenetic effects both in vitro and in vivo.SYHA1813 also displayed potent in vivo antitumor activity against GBM in immune-competent and immune-deficient mouse models,including temozolomide(TMZ)insensitive tumors.Notably,SYHA1813 could penetrate the blood-brain barrier(BBB)and prolong the survival time of mice bearing intracranial GBM xenografts.Moreover,SYHA1813 treatment resulted in a synergistic antitumor efficacy in combination with the PD-1 antibody.As a clinical proof of concept,SYHA1813 achieved confirmed responses in patients with recurrent GBM in an ongoing first-in-human phase I trial.The data of this study support the rationale for an ongoing phase I clinical study(ChiCTR2100045380).
基金the National Natural Science Foundation of China(Grant Nos.11974181,12074386,and 11874358)the Postgraduate Research&Practice Innovation Program of Jiangsu Province(KYCX21_0177)the Top-notch Academic Programs Project of Jiangsu Higher Education Institutions(TAPP),and the Hongque Innovation Center(No.HQ202102003).
文摘Quasi-2D layered Cr4Te5 thin film has attracted great attention because it possesses the high Curie temperature close to room temperature and relatively large saturation magnetization.However,the magnetic interactions and the nature of magnetic phase transition in the Cr4Te5 film have not been explored thoroughly.In this paper,we focused on the critical behavior of its magnetic phase transition through the epitaxial Cr4Te5 film fabricated by pulsed laser deposition(PLD).The final critical exponentsβ=0.359(2)andγ=1.54(2)were obtained by linear extrapolation together with ArrottNoakes equation of state,and their accuracy was confirmed by using the Widom scaling relation and scaling hypothesis.We find that some magnetic disorders exist in the Cr4Te5 film system,which is related to Cr4Te5 critical behavior why its critical behavior is quite far from any conventional universality class.Furthermore,we also determined that the Cr4Te5 film exhibits a quasi-2D long-range magnetic interaction.Finally,the itinerant ferromagnets of Cr4Te5 films were confirmed by the Takahashi’s self-consistent renormalization theory of spin fluctuations.Our work provides a new idea for understanding the mechanism of magnetic interactions in similar 2D layered films.
基金This research was supported by the National Key Research and De-velopment Plan(Grant No.2019YFE0197500)the Scientific Research Foundation of Wuhan University of Technology(Grant No.40120237 and 40120551)the National Natural Science Foundation of China(Grant No.51778511).
文摘The noise reduction effect of noise barriers has been extensively studied,but the effect on pollutant dispersion remains unclear.A computational fluid dynamics(CFD)simulation is conducted to investigate the effects of different heights,lengths,and types of noise barriers and different wind speeds on pollutant dispersion in street canyons with viaducts.The field synergy theory of the convective mass transfer process is used for quantitative analysis of pollutant dispersion in street canyons.The results show that as the height and length of the noise barrier increase,the pollutant dispersion capacity decreases.As the wind speed increases,the rate of decrease in the average CO concentration declines.The effect of the wind speed on the synergistic improvement of the speed and concentration gradient vectors differs for different types of noise barriers.The performance follows the order:fully-closed noise barrier>left noise barrier>right noise barrier>semi-closed noise barrier.The different noise barrier types significantly impact the flow field and pollutant dispersion and reduce the CO concentration to varying degrees,except for the fully-closed type.The average CO concentration in the pedestrian breathing zone is reduced by a maximum of 55.85%on the leeward side and by 53%on the windward side,indicating that an appropriate noise barrier on the viaduct reduces noise pollution and improves the air quality in street canyons,especially in the pedestrian breathing zone.
基金supported by the Special Scientific Research Fund of the Public Welfare Profession of China(201502028)the Excellent Discipline Leader Training Program of Shanghai Health System(No.2017BR037)+2 种基金the NationalNatural Science Foundation of China(No.81772091)Clinical Research Plan of Shanghai Hospital Devel-opment Center(No.SHDC2020CR3039B)Shanghai Rising-Star Program(17QB1403300).
文摘Background:Poor sleep quality is associated with a decrease in quality of life in patients with major burn scars,combined with pruritus and pain.Few interventions have been reported to improve the sleep quality of patients with scars.In the current prospective cohort study,we investigated the efficacy of CO_(2)-ablative fractional laser(AFL)surgery vs conventional surgery in post-burn patients with hypertrophic scars with sleep quality as the primary study outcome.Methods:In total 68 consecutive patients undergoing scar surgical treatment were recruited,including a CO_(2)-AFL surgery cohort(n=35)and a conventional surgery cohort(n=33).A subgroup from the AFL cohort was selected.Sleep quality,pain and pruritus were evaluated.Multiple linear regression analyses were performed to reveal the effect of CO_(2)-AFL surgery.Results:The CO_(2)-AFL surgery cohort had significantly lower Pittsburgh sleep quality index(PSQI)global scores than the conventional surgery cohort after the last surgical treatment.In the subgroup of patients receiving hardware sleep monitoring,CO_(2)-AFL markedly increased deep sleep time,deep sleep efficiency and reduced initial sleep latency.Compared to the conventional surgery cohort,the CO_(2)-AFL cohort presented significantly lower pain and pruritus scores.Correlation analysis showed pain and pruritus were significantly associated with PSQI scores,and there were also significant correlations between pain and pruritus scores.Multiple linear regression analysis showed that surgery method was negatively linearly correlated with visual analog scale(VAS)pain score,brief pain inventory(BPI)total,VAS pruritus score,5-D itch scale total,four-item itch questionnaire(FIIQ)total and PSQI total.Conclusions:CO_(2)-AFL surgery significantly improved sleep quality and reduced pain and pruritus of hypertrophic scar patients.The alleviation of sleep disorder was associated with improvement of deep sleep quality including deep sleep time and deep sleep deficiency.Trial registration:The Chinese Clinical Trial Registry(ChiCTR200035268)approved retrospectively registration on 5 Aug 2020.
文摘In this paper, anisotropic Crouzeix-Raviart type nonconforming finite element meth- ods are considered for solving the second order variational inequality with displacement obstacle. The convergence analysis is presented and the optimal order error estimates are obtained under the hypothesis of the finite length of the free boundary. Numerical results are provided to illustrate the correctness of theoretical analysis.
基金supported in part by National Key Research and Development Program of China(2020YFA0907800 and 2018YFC1706104)National Natural Science Foundation of China(31861133004 and 81502968)+1 种基金the Deutsche Forschungsgemeinschaft(DFG,German Research Foundation Li844/11-1,Germany)Key Research Program of Frontier Sciences,CAS(ZDBS-LY-SM016,China)。
文摘Tyrosine-decahydrofluorene derivatives feature a fused[6.5.6]tricarbocyclic core and a 13-membered para-cyclophane ether.Herein,we identified new xenoacremones A,B,and C(1-3)from the fungal strain Xenoacremonium sinensis ML-31 and elucidated their biosynthetic pathway using gene deletion in the native strain and heterologous expression in Aspergillus nidulans.The hybrid polyketide synthaseenonribosomal peptide synthetase(PKS-NRPS)XenE together with enoyl reductase XenG were confirmed to be responsible for the formation of the tyrosine-nonaketide skeleton.This skeleton was subsequently dehydrated by XenA to afford a pyrrolidinone moiety.XenF catalyzed a novel sigmatropic rearrangement to yield a key cyclohexane intermediate as a prerequisite for the formation of the multi-ring system.Subsequent oxidation catalyzed by XenD supplied the substrate for XenC to link the para-cyclophane ether,which underwent subsequent spontaneous Diels-Alder reaction to give the end products.Thus,the results indicated that three novel enzymes XenF,XenD,and XenC coordinate to assemble the[6.5.6]tricarbocyclic ring and para-cyclophane ether during biosynthesis of complex tyrosine-decahydrofluorene derivatives.