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Macrophage exosomes modified by miR-365-2-5p promoted osteoblast osteogenic differentiation by targeting OLFML1
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作者 caiyao hou Yujue Zhang +7 位作者 Zhaoyong Lv Yurun Luan Jun Li Chunxiu Meng Kun Liu Xin Luo Liyu Chen Fengzhen Liu 《Regenerative Biomaterials》 SCIE EI CSCD 2024年第3期134-147,共14页
In the bone immune microenvironment,immune cells can regulate osteoblasts through a complex communication network.Macrophages play a central role in mediating immune osteogenesis,exosomes derived from them have osteog... In the bone immune microenvironment,immune cells can regulate osteoblasts through a complex communication network.Macrophages play a central role in mediating immune osteogenesis,exosomes derived from them have osteogenic regulation and can be used as cariers in bone tissue engineering.However,there are problems with exosomal therapy alone,such as poor targeting,and the content of loaded molecules cannot reach the therapeutic concentration.In this study,macrophage-derived exosomes modified with miR-365-2-5p were developed to accelerate bone healing.MC3T3-E1 cells were incubated with the culture supermatants of Mo,M1 and M2 macrophages,and it was found that the culture medium of M2 macrophages had the most significant effects in contributing to osteogenesis.High-throughput sequencing identified that miR-365-2-5p was significantly expressed in exosomes derived from M2 macrophages.We incubated MC3T3-E1 with exosomes overexpressing or kmocking down miR-365-2-5p to examine the biological function of exosome miR-365-2-5p on MC3T3-E1 differentiation.These findings suggested that miR-365-2-5p secreted by exosomes increased the osteogenesis of MC3T3-E1.Moreover,miR-365-2-5p had a direct influence over osteogenesis for MC3T3-Ei.Sequencing analysis combined with dual luciferase detection indicated that miR-365-2-5p binded to the 3'-UTR of OLFML1.In summary,exosomes secreted by M2 macrophages targeted OLFML1 through miR-365-2-5p to facilitate osteogenesis. 展开更多
关键词 MACROPHAGE EXOSOMES OSTEOGENESIS OSTEOIMMUNOLOGY
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CaP-coated Zn-Mn-Li alloys regulate osseointegration via influencing macrophage polarization in the osteogenic environment 被引量:1
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作者 Huifen Qiang caiyao hou +7 位作者 Yujue Zhang Xin Luo Jun Li Chunxiu Meng Kun Liu Zhaoyong Lv Ximeng Chen Fengzhen Liu 《Regenerative Biomaterials》 SCIE EI CSCD 2023年第1期1096-1110,共15页
Immune response is an important factor in determining the fate of bone replacement materials,in which macrophages play an important role.It is a new idea to design biomaterials with immunomodulatory function to reduce... Immune response is an important factor in determining the fate of bone replacement materials,in which macrophages play an important role.It is a new idea to design biomaterials with immunomodulatory function to reduce inflammation and promote bone integration by regulating macrophages polarization.In this work,the immunomodulatory properties of CaP Zn-Mn-Li alloys and the specific mechanism of action were investigated.We found that the CaP Zn0.8Mn0.1Li alloy promoted the polarization of macrophages toward M2 and reduced inflammation,which could effectively upregulate osteogenesis-related factors and promote new bone formation,indicating the important role of macrophages polarization in biomaterial induction of osteogenesis.In vivo studies further demonstrated that CaP Zn0.8Mn0.1Li alloy could stimulate osteogenesis better than other Zn-Mn-Li alloys implantations by regulating macrophages polarization and reducing inflammation.In addition,transcriptome results showed that CaP Zn0.8Mn0.1Li played an important regulatory role in the life process of macrophages,activating Toll-like receptor signaling pathway,which participated in the activation and attenuation of inflammation,and accelerated bone integration.Thus,by preparing CaP coatings on the surface of Zn-Mn-Li alloys and combining the bioactive ingredient with controlled release,the biomaterial will be imbibed with beneficial immunomodulatory properties that promote bone integration. 展开更多
关键词 Zn-Mn-Li alloys CaP coatings macrophages polarization OSTEOINTEGRATION inflammation
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MicroRNA-21a-5p-modified macrophage exosomes as natural nanocarriers promote bone regeneration by targeting GATA2 被引量:1
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作者 Xin Luo Chunxiu Meng +7 位作者 Yujue Zhang Qicui Du caiyao hou Huifen Qiang Kun Liu Zhaoyong Lv Jun Li Fengzhen Liu 《Regenerative Biomaterials》 SCIE EI CSCD 2023年第1期1413-1428,共16页
Bone immune responses based on macrophages are critical in the osteogenesis of bone abnormalities.In general,M2 macrophage facilitate the promotion of osteogenesis,as well,M1 macrophage play an important role in early... Bone immune responses based on macrophages are critical in the osteogenesis of bone abnormalities.In general,M2 macrophage facilitate the promotion of osteogenesis,as well,M1 macrophage play an important role in early bone healing,as confirmed by previous studies.However,it is not clear how M1 macrophage are involved in the bone immune response.MiR-21a-5p is a highly expressed microRNA in M1 macrophage in contrast to M2.Therefore,the current work sought to ascertain the influence of M1 macrophage on bone healing via exosomal miR-21a-5p and the probable mechanism.We discovered that injecting M1 macrophage exosomes overexpressing miR-21a-5p into bone defect locations enhanced bone regeneration in vivo.Furthermore,by directly targeting GATA2,miR-21a-5p accelerated MC3T3-E1 osteogenic differentiation.Our findings showed that exosomal miR-21a-5p from M1 macrophage may be transported to osteoblasts and target GATA2 to enhance bone defect healing. 展开更多
关键词 EXOSOMES miR-21a-5p GATA2 MACROPHAGES bone regeneration
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