The clinical characterization of Bacillus amy-loliquefacien contaminating 75%alcohol disinfectants were studied.The bacteria were cultured and observed by using bacterial examination under the hospital infec-tion moni...The clinical characterization of Bacillus amy-loliquefacien contaminating 75%alcohol disinfectants were studied.The bacteria were cultured and observed by using bacterial examination under the hospital infec-tion monitor.According to the regulations,the resistance of bacterial to physical and chemical factors was tested.Drug sensitivity tests for 20 commonly used medicines were carried out using a K-B method.The bacterial plas-mids were analyzed using the Birnboim method.The bac-teria were found after being cultured in the clinically-used 75%alcohol disinfectant fluid.Their total number was more than 800 cfu/mL and they were identified as Bacillus amyloliquefacien.The bacteria were also found to be resistant to boiling for 5 min.It grew well in 95%alcohol disinfectant and was insensitive to 84 disinfectant fluids containing chlorine(1000 mg/L)and such disinfec-tants as ozone.They were able to be sterilized better through routine ultraviolet exposure for 30 min or gas pressure.The bacteria contained a 2.5 kb plasmid and were sensitive to 13 drugs and insensitive to 7 drugs of the 20 drugs tested.It was suggested that alcohol dis-infectant fluid was easily contaminated by Bacillus amy-loliquefacien,and the bacteria was resistant to disinfectant fluids such as alcohol and 84 disinfectants.展开更多
基金This study was supported by a grant from Science Foundation of Health Department in Hubei,China(No.JX2B73).
文摘The clinical characterization of Bacillus amy-loliquefacien contaminating 75%alcohol disinfectants were studied.The bacteria were cultured and observed by using bacterial examination under the hospital infec-tion monitor.According to the regulations,the resistance of bacterial to physical and chemical factors was tested.Drug sensitivity tests for 20 commonly used medicines were carried out using a K-B method.The bacterial plas-mids were analyzed using the Birnboim method.The bac-teria were found after being cultured in the clinically-used 75%alcohol disinfectant fluid.Their total number was more than 800 cfu/mL and they were identified as Bacillus amyloliquefacien.The bacteria were also found to be resistant to boiling for 5 min.It grew well in 95%alcohol disinfectant and was insensitive to 84 disinfectant fluids containing chlorine(1000 mg/L)and such disinfec-tants as ozone.They were able to be sterilized better through routine ultraviolet exposure for 30 min or gas pressure.The bacteria contained a 2.5 kb plasmid and were sensitive to 13 drugs and insensitive to 7 drugs of the 20 drugs tested.It was suggested that alcohol dis-infectant fluid was easily contaminated by Bacillus amy-loliquefacien,and the bacteria was resistant to disinfectant fluids such as alcohol and 84 disinfectants.
