目的探索程序性细胞死亡蛋白4(PDCD4)在脑出血(ICH)后血肿周围域的表达、作用及机制,为ICH疾病的后期康复治疗提供分子靶点。方法 Western blot、免疫组织化学检测蛋白质的表达变化;免疫荧光技术检测蛋白质的空间定位情况;siRNA转染进...目的探索程序性细胞死亡蛋白4(PDCD4)在脑出血(ICH)后血肿周围域的表达、作用及机制,为ICH疾病的后期康复治疗提供分子靶点。方法 Western blot、免疫组织化学检测蛋白质的表达变化;免疫荧光技术检测蛋白质的空间定位情况;siRNA转染进行细胞内蛋白质的干预。结果 PDCD4在大鼠ICH后血肿周围组织中表达上调,最高点位于ICH后第2天;上调的PDCD4主要与神经元Neu N存在共定位,并且与上调的凋亡蛋白活化Caspase-3之间也存在共定位;体外ICH模型中(即hemin诱导的神经元凋亡模型),通过siRNA靶向干预PDCD4的表达,则活化caspase-3明显降低,而存活因子p-Akt上调。结论 ICH后血肿周围域PDCD4的表达上调与神经元的凋亡相关,并且是通过p-Akt通路发挥作用的。展开更多
By a novel controlled combustion synthesis method, a large amount of ZnO nano-whiskers with different morphologies like nanotetrapods, long-leg nanotetrapod and multipods, were prepared without any catalysts and addit...By a novel controlled combustion synthesis method, a large amount of ZnO nano-whiskers with different morphologies like nanotetrapods, long-leg nanotetrapod and multipods, were prepared without any catalysts and additives in open air at high temperature. Their morphologies, structures and optical properties were in-vestigated by using SEM, XRD and PL spectrum. The possible growth mechanisms on the ZnO nano-whiskers were proposed in this paper.展开更多
基金the National Natural Science Foundation of China (Grant Nos. 50572010, 50742007 and 10672020)National Defense Founds of China (Grant Nos. 51420205BQ0154 and A2220061080)+1 种基金"863" Project of China (Grant No. 2007AA03Z103)the Scientific Research Foun-dation of Graduate School of BIT (Grant No. AA200802)
文摘By a novel controlled combustion synthesis method, a large amount of ZnO nano-whiskers with different morphologies like nanotetrapods, long-leg nanotetrapod and multipods, were prepared without any catalysts and additives in open air at high temperature. Their morphologies, structures and optical properties were in-vestigated by using SEM, XRD and PL spectrum. The possible growth mechanisms on the ZnO nano-whiskers were proposed in this paper.
