Study on the Effect and Mechanism of Green Plum Citron Double Strain Fermentation Filtrate on Skin Barrier

To study the effect of Green plum citron double strain fermentation filtrate(DSF)on the proliferation rate of HaCaT keratinocytes by CCK8 assay.The effect of DSF on the mRNA transcription levels of filagolin(FLG),loricrin(LOR),and occludin 1(CLD1)in HaCaT cells in sodium dodecyl sulfate(SLS)model was investigated by qPCR.The effect of DSF on the content of TNF-αin the supernatant of HaCaT cells in the model was investigated by Elisa.The results showed that DSF had no toxicity to HaCaT cells,but it could increase the mRNA transcription levels of FLG,LOR and CLD1 in HaCaT cells in the model,and reduce the content of TNF-αin the supernatant of HaCaT cells in the model.DSF had a certain effect on skin care of strengthening skin barrier.

西安市7所高校学生艾滋病知识知晓率及教育需求

目的了解西安市7所高校学生艾滋病知识知晓率及对艾滋病教育的需求,为合理开展大学生艾滋病健康教育提供科学依据。方法采用多阶段整群抽样方法抽取西安市七所高校12 955名学生进行匿名封闭式问卷调查。结果联合国大会艾滋病特别会议(UNGASS)核心指标问题的绝对正确回答占8.94%;量化赋分的知晓率为18.54%;医学类院校知晓率最高,各高校间有统计学差异;四年级学生的知晓率最高,各年级间有统计学差异。91.03%的人认为学校开展艾滋病健康教育活动应从大一开始;84.34%的人非常和比较愿意参加艾滋病知识培训;参加过的健康教育活动类型最主要的是宣传栏和传单(60.80%);艾滋病知识来源途径主要为互联网(70.44%)等;艾滋病预防和治疗措施(77.23%)等是最希望了解的艾滋病信息;缺乏有效的健康教育模式(53.78%)等是学校艾滋病健康教育的主要不足之处。结论西安市大学生对艾滋病知识的知晓率较低,不及UNGASS的目标和国内防治艾滋病要求。应从学生需求角度出发,有针对地开展健康教育,提高学生艾滋病相关知识知晓率。