3D photoacoustic computed tomography(3D-PACT)has made great advances in volumetric imaging of biological tissues,with high spatial-temporal resolutions and large penetration depth.The development of 3D-PACT requires h...3D photoacoustic computed tomography(3D-PACT)has made great advances in volumetric imaging of biological tissues,with high spatial-temporal resolutions and large penetration depth.The development of 3D-PACT requires high-performance acoustic sensors with a small size,large detection bandwidth,and high sensitivity.In this work,we present a new high-frequency 3D-PACT system that uses a microring resonator(MRR)as the acoustic sensor.The MRR sensor has a size of 80μm in diameter and was fabricated using the nanoimprint lithography technology.Using the MRR sensor,we have developed a transmission-mode 3D-PACT system that has achieved a detection bandwidth of~23 MHz,an imaging depth of~8 mm,a lateral resolution of 114μm,and an axial resolution of 57μm.We have demonstrated the 3D PACT’s performance on in vitro phantoms,ex vivo mouse brain,and in vivo mouse ear and tadpole.The MRR-based 3D-PACT system can be a promising tool for structural,functional,and molecular imaging of biological tissues at depths.展开更多
基金sponsored by American Heart Association Collaborative Sciences Award (18CSA34080277)the United States National Institutes of Health (NIH)grants R21EB027981,R21 EB027304,RF1 NS115581 (BRAIN Initiative),R01 NS111039,and R01 EB028143+2 种基金Chan Zuckerberg Initiative Grant (2020-226178),all to J.YaoNIH grant P41GM135018 was awarded to H.Zhang and C.Sunsupported by the U.S.Department of Energy,Office of Science,under Contract No.DEAC02-06CH11357.
文摘3D photoacoustic computed tomography(3D-PACT)has made great advances in volumetric imaging of biological tissues,with high spatial-temporal resolutions and large penetration depth.The development of 3D-PACT requires high-performance acoustic sensors with a small size,large detection bandwidth,and high sensitivity.In this work,we present a new high-frequency 3D-PACT system that uses a microring resonator(MRR)as the acoustic sensor.The MRR sensor has a size of 80μm in diameter and was fabricated using the nanoimprint lithography technology.Using the MRR sensor,we have developed a transmission-mode 3D-PACT system that has achieved a detection bandwidth of~23 MHz,an imaging depth of~8 mm,a lateral resolution of 114μm,and an axial resolution of 57μm.We have demonstrated the 3D PACT’s performance on in vitro phantoms,ex vivo mouse brain,and in vivo mouse ear and tadpole.The MRR-based 3D-PACT system can be a promising tool for structural,functional,and molecular imaging of biological tissues at depths.
