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Optimization of Nitrogen, Phosphorus and Salt for Lipid Accumulation of Microalgae: Towards the Viability of Microalgae Biodiesel
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作者 carolina t. miranda Daniel V. N. de Lima +2 位作者 Georgia C. Atella Paula F. de Aguiar Sandra M. F. O. Azevedo 《Natural Science》 2016年第12期557-573,共18页
In recent years, microalgae biodiesel has attracted expressive attention and investment, once it was considered a potential resource for energy. Although the wide use of microalgae biodiesel is restricted by its high ... In recent years, microalgae biodiesel has attracted expressive attention and investment, once it was considered a potential resource for energy. Although the wide use of microalgae biodiesel is restricted by its high production cost. For cost-efficient and sustainable production of biodiesel from microalgae, a proper understanding of the variables and their impacts on physiology of the strains is required. In this study, a simple factorial design 23 was used to find optimal conditions for the cultivation of Ankistrodesmus sp. and Chlamydomonas sp. in batch culture. The three components considered were nitrate, phosphate and sodium chloride, used to assess the metabolic versatility of the strains in brackish conditions. The results showed that culture medium with 0.04 g·L?1 nitrate, 0.01 g·L?1 phosphate and 5.0 g·L?1 sodium chloride resulted to be the most effective condition to growth and fatty acids accumulation. Using this optimal condition, Ankistrodesmus sp. and Chlamydomonas sp. increased in 2.1 and 2.4 folds their fatty acids yield, respectively. Importantly, this protocol reduced 75% of the nitrate and phosphate concentrations of the original medium (ASM-1). Additionally, fatty acids analysis found that these strains were mainly constituted of C16-C18, in accordance with the requirements for biodiesel production. The simple factorial design applied here proved to be an important tool towards a better understanding of synergistic effects of tested factors on microalgae metabolism, and the resulting information could be used effectively to improve microalgae cultivation. 展开更多
关键词 OPTIMIZATION MICROALGAE LIPIDS Ankistrodesmus CHLAMYDOMONAS Experimental Design
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Microalgae Lipid and Biodiesel Production: A Brazilian Challenge
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作者 carolina t. miranda Roberta F. Pinto +3 位作者 Daniel V. N. de Lima carolina V. Viegas Simone M. da Costa Sandra M. F. O. Azevedo 《American Journal of Plant Sciences》 2015年第15期2522-2533,共12页
Global increases in atmospheric CO2 and climate change are drawing considerable attention to identify sources of energy with lower environmental impact than those currently in use. Biodiesel production from microalgae... Global increases in atmospheric CO2 and climate change are drawing considerable attention to identify sources of energy with lower environmental impact than those currently in use. Biodiesel production from microalgae lipids can, in the future, occupy a prominent place in energy generation because it represents a sustainable alternative to petroleum-based fuels. Several species of microalgae produce large amounts of lipids per biomass unit. Triacylglycerol is the fatty acid used for biodiesel production and the main source of energy reserves in microalgae. The current literature indicates that nutrient limitations can lead to triacylglycerol accumulation in different species of microalgae. Further efforts in microalgae screening for biodiesel production are needed to discover a native microalgae that will be feasible for biodiesel production in terms of biomass productivity and oil. This revision focuses in the biotechnological potential and viability of biodiesel production from microalgae. Brazil is located in a tropical region with high light rates and adequate average temperatures for the growth of microalgae. The wide availability of bodies of water and land will allow the country to produce renewable energy from microalgae. 展开更多
关键词 BIODIESEL MICROALGAE LIPIDS CULTIVATION
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