There was a mistake in affiliation a,the correct one should be“MOE Key Laboratory of Marine Genetics and Breeding and Sars-Fang Center,Ocean University of China,Qingdao 266003,China”as updated above;Affiliations c a...There was a mistake in affiliation a,the correct one should be“MOE Key Laboratory of Marine Genetics and Breeding and Sars-Fang Center,Ocean University of China,Qingdao 266003,China”as updated above;Affiliations c and d should be reversed:Affiliation c should be“Laboratory for Marine Fisheries Science and Food Production Processes,Pilot Qingdao National Laboratory for Marine Science and Technology,Qingdao 266237,China,”and the affiliation d should be“Laboratory of Tropical Marine Germplasm Resources and Breeding Engineering,Sanya Oceanographic Institution,Ocean University of China,Sanya 572000,China.”展开更多
Targeted genotyping is an extremely powerful approach for the detection of known genetic variations that are biologically or clinically important.However,for non-model organisms,large-scale target geno-typing in a cos...Targeted genotyping is an extremely powerful approach for the detection of known genetic variations that are biologically or clinically important.However,for non-model organisms,large-scale target geno-typing in a cost-effective manner remains a major challenge.To address this issue,we present an ultrahigh-multiplex,in-solution probe array-based high-throughput diverse marker genotyping(HD-Marker)approach that is capable of targeted genotyping of up to 86000 loci,with coverage of the whole gene repertoire,in what is a 27-fold and six-fold multiplex increase in comparison with the conventional Illumina GoldenGate and original HD-Marker assays,respectively.We perform extensive analyses of var-ious ultrahigh-multiplex levels of HD-Marker(30 k-plex,56 k-plex,and 86 k-plex)and show the power and excellent performance of the proposed method with an extremely high capture rate(about 96%)and genotyping accuracy(about 96%).With great advantages in terms of cost(as low as 0.0006 USD per geno-type)and high technical flexibility,HD-Marker is a highly efficient and powerful tool with broad appli-cation potential for genetic,ecological,and evolutionary studies of non-model organisms.展开更多
文摘There was a mistake in affiliation a,the correct one should be“MOE Key Laboratory of Marine Genetics and Breeding and Sars-Fang Center,Ocean University of China,Qingdao 266003,China”as updated above;Affiliations c and d should be reversed:Affiliation c should be“Laboratory for Marine Fisheries Science and Food Production Processes,Pilot Qingdao National Laboratory for Marine Science and Technology,Qingdao 266237,China,”and the affiliation d should be“Laboratory of Tropical Marine Germplasm Resources and Breeding Engineering,Sanya Oceanographic Institution,Ocean University of China,Sanya 572000,China.”
基金the grant support from National Natural Science Foundation of China (32130107, 32002446 and 32102778)Project of Sanya Yazhouwan Science and Technology City Management Foundation (SKJC-KJ-2019KY01)+1 种基金China Agriculture Research System of MOF and MARATaishan Scholar Project Fund of Shandong Province of China
文摘Targeted genotyping is an extremely powerful approach for the detection of known genetic variations that are biologically or clinically important.However,for non-model organisms,large-scale target geno-typing in a cost-effective manner remains a major challenge.To address this issue,we present an ultrahigh-multiplex,in-solution probe array-based high-throughput diverse marker genotyping(HD-Marker)approach that is capable of targeted genotyping of up to 86000 loci,with coverage of the whole gene repertoire,in what is a 27-fold and six-fold multiplex increase in comparison with the conventional Illumina GoldenGate and original HD-Marker assays,respectively.We perform extensive analyses of var-ious ultrahigh-multiplex levels of HD-Marker(30 k-plex,56 k-plex,and 86 k-plex)and show the power and excellent performance of the proposed method with an extremely high capture rate(about 96%)and genotyping accuracy(about 96%).With great advantages in terms of cost(as low as 0.0006 USD per geno-type)and high technical flexibility,HD-Marker is a highly efficient and powerful tool with broad appli-cation potential for genetic,ecological,and evolutionary studies of non-model organisms.
