We proposed a new saccharides sensor developed by symmetrical optical waveguide(SOW)-based surface plasmon resonance(SPR).This unique MgF_(2)/Au/MgF_(2)/Analyte film structure results in longer suface plasmon wave(SPW...We proposed a new saccharides sensor developed by symmetrical optical waveguide(SOW)-based surface plasmon resonance(SPR).This unique MgF_(2)/Au/MgF_(2)/Analyte film structure results in longer suface plasmon wave(SPW)propagation lengths and depths,leading to an increment of resolution.In this paper,we managed to decorate the dielectric interface(MgF_(2) layer)by depositing a thin polydopamine film as surface adherent that provides a platform for secondary reactions with the probe molecule.3-Aminophenylboronic acid(3-PBA)is chosen to be the saccharides sense probe molecule in the present work.The aqueous huumnor of Diabetes and Cataract patient whose blood glucose level is normal are analyzed and the results dermonstrated that this sensor shows great potential in monitoring the blood siugar and can be adapted in the field of biological monitoring in the future.展开更多
We developed a biosensor that is capable for simultaneous surface plasmon resonance(SPR)sensing and hyperspectral fuores cence analysis in this paper.A symmetrical metal-dielectric slabscheme is employed for the excit...We developed a biosensor that is capable for simultaneous surface plasmon resonance(SPR)sensing and hyperspectral fuores cence analysis in this paper.A symmetrical metal-dielectric slabscheme is employed for the excitation of coupled plasnon waveguide resonance(CPWR)in thepresent work.Resonance bet ween surface plasmon mode and the guided waveguide mode gen-erates narrower full width half-maximum of the refective curves which leads to increased pre.cision for the determination of refractive index over conventional SPR sensors.In addition,CPWR also fers longer surface propagation depths and higher surface electric field strengthsthat enable the excitation of fluorescence with hyperspectral technique to maintain an appreci-able signal-to-noise ratio.The refractive index information obtained from SPR sensing and thechemical properties obt ained through hyperspectral fluorescence analysis confirm each other toexclude false-positive or false-negative cases.The sensor provides a comprehensive understandingof the biological events on the sensor chips.展开更多
基金support from NSFC China (grants 61275188,61378089 and 81171375)the key project of Guangdong province (2012A080203008)+1 种基金the Basic Research Program of Shenzhen City (JC201105201121A)State Key Laboratory Open Foundation Issue,China (grant 12K05ESPCT).
文摘We proposed a new saccharides sensor developed by symmetrical optical waveguide(SOW)-based surface plasmon resonance(SPR).This unique MgF_(2)/Au/MgF_(2)/Analyte film structure results in longer suface plasmon wave(SPW)propagation lengths and depths,leading to an increment of resolution.In this paper,we managed to decorate the dielectric interface(MgF_(2) layer)by depositing a thin polydopamine film as surface adherent that provides a platform for secondary reactions with the probe molecule.3-Aminophenylboronic acid(3-PBA)is chosen to be the saccharides sense probe molecule in the present work.The aqueous huumnor of Diabetes and Cataract patient whose blood glucose level is normal are analyzed and the results dermonstrated that this sensor shows great potential in monitoring the blood siugar and can be adapted in the field of biological monitoring in the future.
基金the financial support from NSFC Chima(grants 61275188,81171375 and 61361160416)the key prjet of Guangdong province(2012A080203008)+1 种基金the Basic Research Pro-gram of Shenzhen City(jC20110520121A)State Key Laboratory Open Foundation Issue,China(grant 12K05ESPCT).
文摘We developed a biosensor that is capable for simultaneous surface plasmon resonance(SPR)sensing and hyperspectral fuores cence analysis in this paper.A symmetrical metal-dielectric slabscheme is employed for the excitation of coupled plasnon waveguide resonance(CPWR)in thepresent work.Resonance bet ween surface plasmon mode and the guided waveguide mode gen-erates narrower full width half-maximum of the refective curves which leads to increased pre.cision for the determination of refractive index over conventional SPR sensors.In addition,CPWR also fers longer surface propagation depths and higher surface electric field strengthsthat enable the excitation of fluorescence with hyperspectral technique to maintain an appreci-able signal-to-noise ratio.The refractive index information obtained from SPR sensing and thechemical properties obt ained through hyperspectral fluorescence analysis confirm each other toexclude false-positive or false-negative cases.The sensor provides a comprehensive understandingof the biological events on the sensor chips.
