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Bioefficacy of crude extract of Cyperus aromaticus(Family:Cyperaceae)cultured cells,against Aedes aegypti and Aedes albopictus mosquitoes
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作者 Fatemeh Kamiabi Zairi Jaal chan lai keng 《Asian Pacific Journal of Tropical Biomedicine》 SCIE CAS 2013年第10期767-775,共9页
Objective:To evaluate the growth inhibition activity of the crude extract of Cyperus aromaticus(C.aromaticus)cultured cells against the 3rd instar larvae of Aedes aegypti(Linn.)and Aedes albopictus Skuse(Ae.albopictus... Objective:To evaluate the growth inhibition activity of the crude extract of Cyperus aromaticus(C.aromaticus)cultured cells against the 3rd instar larvae of Aedes aegypti(Linn.)and Aedes albopictus Skuse(Ae.albopictus)under laboratory conditions,and determine the sublethal effects(EI_(50))of the crude extract of C.aromaticus cultured cells on some biological and morphological parameters of both Aedes mosquito species during two generations as well.Methods:The cell suspension cultures of C.aromaticus were activated from five callus lines(P4,Pa,Z1,Z6 and Ml)derived from the root explants of in vitro plantlets.The cultured cells were extracted in chloroform and used as plant material for the present study.For detection of juvenile hormone III.the crude extracts were analyzed by HPLC.Then the crude extracts of the three C.aromaticus cultured cell lines which contained varied amounts of juvenile hormone III[high level(P4 cell line),medium level(Z1 cell line)and low level(Ml cell line)]were tested against Aedes mosquito species.Laboratory evaluation was performed against late third instar larvae of the Vector Control Research Unit strains of Ae.aegypti and Ae.albopictus using the standard WHO method.The effects of EI_(50)of the C.aromaticus cultured P4 cells on fecundity,fertility,growth period,sex ratio,adult size and longevity of Aedes mosquitoes were assessed.Results:Bioassay tests presented the remarkable growth inhibition activity of the crude extracts of C.aromaticus cultured cells against the two Aedes mosquitoes.Between the two mosquito species,Ae.albopictus was more susceptible to the crude extracts with lower EI_(50)values.EI_(50)of the crude extract of C.aromaticus cultured cells(P4)increased the sterility indices in the parental generation females in both Aedes mosquito species.A significant delay in the pupal formation and adult emergence were observed in the parental generation of the both mosquito species.The sex ratio of the adult population either parental or F1 generation of the Aedes mosquito species was not significantly affected by the EI_(50)dosage of the crude extract of C.aromaticus cultured P4 cells.A significant decrease in the wing length of the treated adult(female and male)of Aedes aegypti as well as the treated female of Ae.albopictus were observed.Longevity of the adult female of the parental generation of both Aedes mosquitoes as well as females of F1 generation of Ae.albopictus were significantly decreased.Conclusions:The present study revealed the potential of the crude extract of C.aromaticus cultured cells in controlling vector mosquito populations in the effort to reduce the transmission of vector borne diseases. 展开更多
关键词 AEDES aegypti AEDES ALBOPICTUS CYPERUS aromaticus Plant cell culture JUVENILE hormone Emergence inhibition
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番木瓜无性繁殖的问题与展望
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作者 chan lai keng 陈传琴 《世界热带农业信息》 1989年第1期16-16,共1页
本文简要地叙述了番木瓜组织培养的成绩、现状和存在问题。
关键词 无性繁殖 小植株 实生苗 番木瓜 组织培养 外植体 体细胞胚 固体培养基 愈伤组织诱导 茎尖培养
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