Nerve injury repair has been an intractable issue wor.ldwide due to the non-renew able property of neurons in vivo.Cell therapy adopting me senchymal stem cells(MSC)induced neurons stands for a promising substitute tr...Nerve injury repair has been an intractable issue wor.ldwide due to the non-renew able property of neurons in vivo.Cell therapy adopting me senchymal stem cells(MSC)induced neurons stands for a promising substitute treatment,which is hindered by the low diffe rentiation efficiency of current approaches.To improve the efficiency of MSC differentiation in vitro,we constructed a three-dimensional(3D)culture system by seeding human bone marrow-de rived MSC(hBMSCs)into collagen-nano fiber scaffolds which are a mixed hydrogel of collagen 1 and silk nanofiber solution and testified the diffe rentiation efficiency from MSC into neurons by chemical or cocktail induction methods.Results indicated MSCs cultured in 3D system showed good viability with neuron-like morphology in both groups.展开更多
文摘Nerve injury repair has been an intractable issue wor.ldwide due to the non-renew able property of neurons in vivo.Cell therapy adopting me senchymal stem cells(MSC)induced neurons stands for a promising substitute treatment,which is hindered by the low diffe rentiation efficiency of current approaches.To improve the efficiency of MSC differentiation in vitro,we constructed a three-dimensional(3D)culture system by seeding human bone marrow-de rived MSC(hBMSCs)into collagen-nano fiber scaffolds which are a mixed hydrogel of collagen 1 and silk nanofiber solution and testified the diffe rentiation efficiency from MSC into neurons by chemical or cocktail induction methods.Results indicated MSCs cultured in 3D system showed good viability with neuron-like morphology in both groups.
