The failure of cement sheath integrity can be easily caused by alternating pressure during large-scale multistage hydraulic fracturing in shale-gas well.An elastic-plastic mechanical model of casing-cement sheath-form...The failure of cement sheath integrity can be easily caused by alternating pressure during large-scale multistage hydraulic fracturing in shale-gas well.An elastic-plastic mechanical model of casing-cement sheath-formation(CSF)system under alternating pressure is established based on the Mohr-Coulomb criterion and thick-walled cylinder theory,and it has been solved by MATLAB programming combining global optimization algorithm with Global Search.The failure mechanism of cement sheath integrity is investigated,by which it can be seen that the formation of interface debonding is mainly related to the plastic strain accumulation,and there is a risk of interface debonding under alternating pressure,once the cement sheath enters plasticity whether in shallow or deep well sections.The matching relationship between the mechanical parameters(elastic modulus and Poisson's ratio)of cement sheath and its integrity failure under alternating pressure in whole well sections is studied,by which it has been found there is a“critical range”in the Poisson's ratio of cement sheath.When the Poisson's ratio is below the“critical range”,there is a positive correlation between the yield internal pressure of cement sheath(SYP)and its elastic modulus.However,when the Poisson's ratio is above the“critical range”,there is a negative correlation.The elastic modulus of cement sheath is closely related to its Poisson's ratio,and restricts each other.Scientific and reasonable matching between mechanical parameters of cement sheath and CSF system under different working conditions can not only reduce the cost,but also protect the cement sheath integrity.展开更多
Objective:Spontaneous hepatocellular carcinoma(HCC)rupture can be fatal,and hepatic resection could achieve a favorable long-term survival among all strategies of tumor rupture.However,there is no available prognostic...Objective:Spontaneous hepatocellular carcinoma(HCC)rupture can be fatal,and hepatic resection could achieve a favorable long-term survival among all strategies of tumor rupture.However,there is no available prognostic scoring system for patients with ruptured HCC who underwent partial hepatectomy.Methods:From January 2005 to May 2015,129 patients with spontaneous HCC rupture underwent partial hepatectomy.Preoperative clinical data were collected and analyzed.Independent risk factors affecting overall survival(OS)were used to develop the new scoring system.Harrell’s C statistics,Akaike information criterion(AIC),the relative likelihood,and the log likelihood ratio were calculated to measure the homogeneity and discriminatory ability of a prognostic system.Results:In the multivariable Cox regression analysis,three factors,including tumor size,preoperativeα-fetoprotein level,and alkaline phosphatase level,were chosen for the new tumor-associated antigen(TAA)prognostic scoring system.The 1-year OS rates were 88.1%,43.2%,and 30.2%for TAA scores of 0–5 points(low-risk group),6–9 points(moderate-risk group),and 10–13points(high-risk group),respectively.The TAA scoring system had superior homogeneity and discriminatory ability(Harrell’s C statistics,0.693 vs.0.627 and 0.634;AIC,794.79 vs.817.23 and 820.16;relative likelihood,both<0.001;and log likelihood ratio,45.21 vs.22.77 and 21.84)than the Barcelona Clinic Liver Cancer staging system and the Cancer of the Liver Italian Program in predicting OS.Similar results were found while predicting disease-free survival(DFS).Conclusions:The new prognostic scoring system is simple and effective in predicting both OS and DFS of patients with spontaneous ruptured HCC.展开更多
An improved single image dehazing method based on dark channel prior and wavelet transform is proposed. This pro-posed method employs wavelet transform and guided filter instead of the soft matting procedure to estima...An improved single image dehazing method based on dark channel prior and wavelet transform is proposed. This pro-posed method employs wavelet transform and guided filter instead of the soft matting procedure to estimate and refine the depth map of haze images. Moreover, a contrast enhancement method based on just noticeable difference (JND) and quadratic function is adopted to enhance the contrast for the dehazed image, since the scene radiance is usual y not as bright as the atmospheric light, and the dehazed image looks dim. The experimental results show that the proposed approach can effectively enhance the haze ima-ge and is wel suitable for implementing on the surveil ance and obstacle detection systems.展开更多
Introduction:Parallel stent-stent grafting is a major endovascular technique used to preserve the supra-aortic branches during thoracic endovascular aortic repair(TEVAR)of aortic pathologies involving the aortic arch....Introduction:Parallel stent-stent grafting is a major endovascular technique used to preserve the supra-aortic branches during thoracic endovascular aortic repair(TEVAR)of aortic pathologies involving the aortic arch.The short-and mid-term results of this technique are satisfactory;however,endoleak remains a major concern.Thus,here we designed a new chimney stent-graft to decrease the endoleak rate.Aim:To testify the feasibility and safety of the new chimney stent-graft system in a canine model.Material and methods:Six Labrador retrievers were used.Pre-operative data were collected and all operations were performed under general anesthesia.The main and chimney stent-grafts were implanted through the abdominal aorta and left subclavian artery approaches,respectively.Completion digital subtraction angiography(DSA)was performed to confirm the immediate outcomes.All dogs were fed separately for 6 months and sacrificed after aortic angiography.The thoracic aorta and the main and chimney stent-grafts were harvested for histopathologic examination.Results:No complications were found in follow-up DSA.All branch arteries were patent.In?ammatory responses were observed around the stent-grafts in 3 experimental animals,and slight hyperplasia was observed in the surrounding tissues compared with the normal vessels.There was no mural thrombus in the stent,endothelial cells were noted on the inner surface of the stent,and thrombus was formed in the outer skirt and gutter area.The histopathologic examinations revealed similar results to those of gross necropsy observations.Conclusions:This study demonstrated the feasibility and safety of the Longuette stent-graft and the first to report a revised stent-graft specific for chimney technique.展开更多
Nanoparticle-mediated drug delivery is recognized as a promising option for targeted treatment of atherosclerosis. In this paper, the Eulerian-Lagrangian technique is adopted to simulate the delivery of drug-loaded na...Nanoparticle-mediated drug delivery is recognized as a promising option for targeted treatment of atherosclerosis. In this paper, the Eulerian-Lagrangian technique is adopted to simulate the delivery of drug-loaded nanoparticles to patient-specific atherosclerotic plaque with the aid of an external magnetic field. Plaques and vascular walls are introduced as porous media formulated by the Darcy-Forchheimer model in this targeted transport process. The results demonstrate that the delivery efficiency of particles to atherosclerosis depends on the external magnetic field, such as configuration and intensity, in which the configuration angle of the current wire is a key factor and the double current wires have advantages over the single current wire. Meanwhile, the delivery efficiency gradually decreases as the distance between the plaque cap and the current wire increases. Further, although augmenting the current or magnetic susceptibility can generally improve the delivery efficiency of nanoparticles, this increase is not apparent when small-sized nanoparticles are employed as drug transport particles. The results obtained can potentially serve as the guideline to optimize regimens for the targeted therapy of atherosclerosis.展开更多
A coupled immersed boundary-lattice Boltzmann method (IB-LBM) is introduced to solve biomimetic problems. Compared to the conventional IB-LBM, the strict satisfaction of no-slip boundary condition is implemented in ...A coupled immersed boundary-lattice Boltzmann method (IB-LBM) is introduced to solve biomimetic problems. Compared to the conventional IB-LBM, the strict satisfaction of no-slip boundary condition is implemented in the current method. Consequently, the phenomenon of flow penetration that is frequently observed in the conventional IB-LBM is fully prevented, and subsequently the force on the boundary can be calculated more accurately. This feature is of importance for the simulation of biomimetic problems. Moreover, by applying the relationship between the velocity correction and forcing term, the boundary force can be calculated easily. Several biomimetic problems are then simulated. Based on the good agreement between the current results and those in the literature, it may be concluded that the present IB-LBM has the capabilitv to handle various biomimetic oroblems.展开更多
The analysis of growth bands in the eyestalk has been increasingly used for estimating crustacean ageing and molting.In this study,we developed an effective method to process and observe the eyestalk microstructure of...The analysis of growth bands in the eyestalk has been increasingly used for estimating crustacean ageing and molting.In this study,we developed an effective method to process and observe the eyestalk microstructure of the swimming crab(Portunus trituberculatus).We found that dark pigmentation as a result of boiling has an influence on the observation of the eyestalk microstructure.Choosing an unboiled eyestalk,this study compared the cross section and longitudinal section,and concluded that the cross section is suitable for the observation of growth increments with 6.1%CV(coefficient of variation),and growth bands are suitable for the observation of the longitudinal section with 5.4%CV.The width of growth increments near the edge of the endocuticle is small,and the width of growth increments of the middle part of the endocuticle is large.Relationship of number of growth bands to molting time was fitted to a linear function with the slope not significantly different from 1,indicating that growth bands are formed associated with molting.Periodicity of growth increment formation was calculated as 3.7 d,however was not verified.Our results provide a new improved technique for identification of crustacean molting and growth.展开更多
In this paper,we use machine learning techniques to form a cancer cell model that displays the growth and promotion of synaptic and electrical signals.Here,such a technique can be applied directly to the spiking neura...In this paper,we use machine learning techniques to form a cancer cell model that displays the growth and promotion of synaptic and electrical signals.Here,such a technique can be applied directly to the spiking neural network of cancer cell synapses.The results show that machine learning techniques for the spiked network of cancer cell synapses have the powerful function of neuron models and potential supervisors for different implementations.The changes in the neural activity of tumor microenvironment caused by synaptic and electrical signals are described.It can be used to cancer cells and tumor training processes of neural networks to reproduce complex spatiotemporal dynamics and to mechanize the association of excitatory synaptic structures which are between tumors and neurons in the brain with complex human health behaviors.展开更多
A simple and rapid method using liquid chromatography–mass spectrometry(LC-MS) for the determination of metaxalone in human plasma has been developed and validated.Letrozole was used as the internal standard(IS). The...A simple and rapid method using liquid chromatography–mass spectrometry(LC-MS) for the determination of metaxalone in human plasma has been developed and validated.Letrozole was used as the internal standard(IS). The plasma samples were simply treated with acetonitrile which allowed the precipitation of plasma proteins. The chromatographic separation was achieved on a Sapphire C18(2.1 mm × 150 mm, 5 μm, Newark, USA)column using the mobile phase(5 mM ammonium acetate containing 0.01% formic acid:acetonitrile(45:55, v/v)) at a flow rate of 0.3 ml/min. The selected ion monitoring(SIM) in the positive mode was used for the determination of [M + H]+m/z 222.1 and 286.1 for metaxalone and letrozole, respectively. The standard curve obtained was linear(r2≥ 0.99)over the concentration range of 30.24-5040 ng/ml. Meanwhile, no interfering peaks or matrix effect was observed. The method established was simple and successfully applied to a pharmacokinetic study of metaxalone in healthy Chinese volunteers after a single oral dose administration of 800 mg metaxalone. The main pharmacokinetic parameters of metaxalone were as follow: Cmax,(1664 ± 1208) ng/ml and(2063 ± 907) ng/ml;AUC0-36,(13925 ± 6590) ng/ml h and(18620 ± 5717) ng/ml h;t1/2,(13.6 ± 7.7) h and(20.3 ± 7.7) h for the reference and test tablets, respectively. These pharmacokinetic parameters of metaxalone in healthy Chinese volunteers were reported for the first time.展开更多
Objective: To evaluate the effectiveness of transvaginal cervical cerclage in singleton pregnancies with cervical incompetence, determine the predictive factors of success and failure, and then compare elective and em...Objective: To evaluate the effectiveness of transvaginal cervical cerclage in singleton pregnancies with cervical incompetence, determine the predictive factors of success and failure, and then compare elective and emergency cerclage. Study Design: This was a retrospective study of 62 patients who underwent cervical cerclage in The First Hospital of Jilin University, China, between May 2015 and January 2018. Successful group was defined as those who delivered live babies and failure group who experienced abortion or stillbirth. Results: Out of 104 patients, 62 met inclusion criteria. In 62 cases, 47 (75.8%) succeeded and 15 (24.2%) failed. In successful group, 21 (44.7%) women delivered pretermly and 26 (55.3%) termly. No severe complications occurred except cervical laceration (2, 3.23%), premature rupture of membranes (13, 20.97%). In 62 cases, 40 (64.5%) have ≤2 previous second-trimester losses and 22 (35.5%) have >2 previous second-trimester losses. No significant differences were found in neonatal outcomes. Analysis revealed that higher postoperative C-reactive protein and presence of premature rupture of membranes were the strongest predictors of cerclage failure. Among 62 cases, 48 (77.4%) were allocated in elective and 14 (22.6%) in emergency cerclage. Pregnancy prolongation was significantly more (P = 0.014) in elective group with no significant differences in premature rupture of membranes, neonatal outcomes (all P > 0.05) except Apgar score at 5 min (P = 0.042). Conclusion: Achieving 75.8% live births proves that transvaginal cervical cerclage is an effective and safe technique in prolonging the gestational age, improving the obstetric outcomes in singleton pregnancies with cervical incompetence under various cerclage indications. Postoperative C-reactive protein and premature rupture of membranes are the predictive factors related to success or failure. Elective cerclage is more effective in prolonging the pregnancy compared to emergency cerclage, no significant differences are seen regarding neonatal outcomes and complications.展开更多
Objective:autism spectrum disorder(ASD),a serious disorder that begins early in life,con-tinues throughout the lifespan.Children with ASD who are diagnosed early are more responsive to therapeutic interventions and ha...Objective:autism spectrum disorder(ASD),a serious disorder that begins early in life,con-tinues throughout the lifespan.Children with ASD who are diagnosed early are more responsive to therapeutic interventions and have less social and language impair-ment than children diagnosed later;however,current di-agnostic measures are mostly applied to children older than one year and lack the appropriate biological markers for early diagnosis of ASD.Using bioinformatic analy-sis,this study explores the molecular information mech-anism of ASD.Method:in this study,we used dataset GSE6575 from Gene Expression Omnibus(GEO)to an-alyze the mRNA expression profile of ASD,including 35 ASD samples and 12 normal control samples looking for different genes and we did enrichment analysis of those genes.We then used the STRING database to construct a protein-protein interaction(PPI)network of differential genes.Finally,Cytoscape plug-in cytoHubba was used to search for hub genes.The diagnostic value of the hub genes was verified by subject operating characteristic curves.Result:we looked for 50 different genes and did an enrichment analysis of those genes.The results of the enrichment analysis showed that these differential genes were mainly concentrated in the response to viruses,the immune regulation of inflammation and energy metabo-lism.Using Cytoscape plug-in cytoHubba,we found ten different genes.We drew ROC curves for all ten genes among which two genes,interleukin 2 receptor subunit beta(IL2Rβ)and perforin 1(PRF1),had good sensitivity and specificity for the early diagnosis of autism.The ar-eas under the ROC curves were 0.855,0.830 for IL2Rβ,PRF1.Conclusion:data analysis using the GEO data-base can provide new insights into the etiology of ASD as well as some possible biomarkers and therapeutic targets for early diagnosis and treatment of ASD.展开更多
基金Research work was financed by the National Natural Science Foundation of China(No.52074232)Sichuan Science and Technology Program(No.2022NSFSC0028,No.2022NSFSC0994).Without their support,this work would not have been possible.
文摘The failure of cement sheath integrity can be easily caused by alternating pressure during large-scale multistage hydraulic fracturing in shale-gas well.An elastic-plastic mechanical model of casing-cement sheath-formation(CSF)system under alternating pressure is established based on the Mohr-Coulomb criterion and thick-walled cylinder theory,and it has been solved by MATLAB programming combining global optimization algorithm with Global Search.The failure mechanism of cement sheath integrity is investigated,by which it can be seen that the formation of interface debonding is mainly related to the plastic strain accumulation,and there is a risk of interface debonding under alternating pressure,once the cement sheath enters plasticity whether in shallow or deep well sections.The matching relationship between the mechanical parameters(elastic modulus and Poisson's ratio)of cement sheath and its integrity failure under alternating pressure in whole well sections is studied,by which it has been found there is a“critical range”in the Poisson's ratio of cement sheath.When the Poisson's ratio is below the“critical range”,there is a positive correlation between the yield internal pressure of cement sheath(SYP)and its elastic modulus.However,when the Poisson's ratio is above the“critical range”,there is a negative correlation.The elastic modulus of cement sheath is closely related to its Poisson's ratio,and restricts each other.Scientific and reasonable matching between mechanical parameters of cement sheath and CSF system under different working conditions can not only reduce the cost,but also protect the cement sheath integrity.
基金the Major Project of the Ministry of Science and Technology of Changsha,China(No.kh2003014)the Hunan Provincial Natural Science Foundation,China(Nos.2018JJ2584,2018JJ3507)+1 种基金the Beijing Municipal Science and Technology Comission,China(No.D171100002917004)the Guangxi Science and Technology Plan Project,China(No.AD16380019).
文摘Objective:Spontaneous hepatocellular carcinoma(HCC)rupture can be fatal,and hepatic resection could achieve a favorable long-term survival among all strategies of tumor rupture.However,there is no available prognostic scoring system for patients with ruptured HCC who underwent partial hepatectomy.Methods:From January 2005 to May 2015,129 patients with spontaneous HCC rupture underwent partial hepatectomy.Preoperative clinical data were collected and analyzed.Independent risk factors affecting overall survival(OS)were used to develop the new scoring system.Harrell’s C statistics,Akaike information criterion(AIC),the relative likelihood,and the log likelihood ratio were calculated to measure the homogeneity and discriminatory ability of a prognostic system.Results:In the multivariable Cox regression analysis,three factors,including tumor size,preoperativeα-fetoprotein level,and alkaline phosphatase level,were chosen for the new tumor-associated antigen(TAA)prognostic scoring system.The 1-year OS rates were 88.1%,43.2%,and 30.2%for TAA scores of 0–5 points(low-risk group),6–9 points(moderate-risk group),and 10–13points(high-risk group),respectively.The TAA scoring system had superior homogeneity and discriminatory ability(Harrell’s C statistics,0.693 vs.0.627 and 0.634;AIC,794.79 vs.817.23 and 820.16;relative likelihood,both<0.001;and log likelihood ratio,45.21 vs.22.77 and 21.84)than the Barcelona Clinic Liver Cancer staging system and the Cancer of the Liver Italian Program in predicting OS.Similar results were found while predicting disease-free survival(DFS).Conclusions:The new prognostic scoring system is simple and effective in predicting both OS and DFS of patients with spontaneous ruptured HCC.
基金This research was supported by the Natural Science Foundation of Fujian Province under Grant Nos. 2015J01012 and 2015J01019.
文摘An improved single image dehazing method based on dark channel prior and wavelet transform is proposed. This pro-posed method employs wavelet transform and guided filter instead of the soft matting procedure to estimate and refine the depth map of haze images. Moreover, a contrast enhancement method based on just noticeable difference (JND) and quadratic function is adopted to enhance the contrast for the dehazed image, since the scene radiance is usual y not as bright as the atmospheric light, and the dehazed image looks dim. The experimental results show that the proposed approach can effectively enhance the haze ima-ge and is wel suitable for implementing on the surveil ance and obstacle detection systems.
文摘Introduction:Parallel stent-stent grafting is a major endovascular technique used to preserve the supra-aortic branches during thoracic endovascular aortic repair(TEVAR)of aortic pathologies involving the aortic arch.The short-and mid-term results of this technique are satisfactory;however,endoleak remains a major concern.Thus,here we designed a new chimney stent-graft to decrease the endoleak rate.Aim:To testify the feasibility and safety of the new chimney stent-graft system in a canine model.Material and methods:Six Labrador retrievers were used.Pre-operative data were collected and all operations were performed under general anesthesia.The main and chimney stent-grafts were implanted through the abdominal aorta and left subclavian artery approaches,respectively.Completion digital subtraction angiography(DSA)was performed to confirm the immediate outcomes.All dogs were fed separately for 6 months and sacrificed after aortic angiography.The thoracic aorta and the main and chimney stent-grafts were harvested for histopathologic examination.Results:No complications were found in follow-up DSA.All branch arteries were patent.In?ammatory responses were observed around the stent-grafts in 3 experimental animals,and slight hyperplasia was observed in the surrounding tissues compared with the normal vessels.There was no mural thrombus in the stent,endothelial cells were noted on the inner surface of the stent,and thrombus was formed in the outer skirt and gutter area.The histopathologic examinations revealed similar results to those of gross necropsy observations.Conclusions:This study demonstrated the feasibility and safety of the Longuette stent-graft and the first to report a revised stent-graft specific for chimney technique.
基金Project supported by the National Natural Science Foundation of China(Nos.11772046 and 81870345)。
文摘Nanoparticle-mediated drug delivery is recognized as a promising option for targeted treatment of atherosclerosis. In this paper, the Eulerian-Lagrangian technique is adopted to simulate the delivery of drug-loaded nanoparticles to patient-specific atherosclerotic plaque with the aid of an external magnetic field. Plaques and vascular walls are introduced as porous media formulated by the Darcy-Forchheimer model in this targeted transport process. The results demonstrate that the delivery efficiency of particles to atherosclerosis depends on the external magnetic field, such as configuration and intensity, in which the configuration angle of the current wire is a key factor and the double current wires have advantages over the single current wire. Meanwhile, the delivery efficiency gradually decreases as the distance between the plaque cap and the current wire increases. Further, although augmenting the current or magnetic susceptibility can generally improve the delivery efficiency of nanoparticles, this increase is not apparent when small-sized nanoparticles are employed as drug transport particles. The results obtained can potentially serve as the guideline to optimize regimens for the targeted therapy of atherosclerosis.
基金supported by the National Natural Science Foundation of China(11272153)
文摘A coupled immersed boundary-lattice Boltzmann method (IB-LBM) is introduced to solve biomimetic problems. Compared to the conventional IB-LBM, the strict satisfaction of no-slip boundary condition is implemented in the current method. Consequently, the phenomenon of flow penetration that is frequently observed in the conventional IB-LBM is fully prevented, and subsequently the force on the boundary can be calculated more accurately. This feature is of importance for the simulation of biomimetic problems. Moreover, by applying the relationship between the velocity correction and forcing term, the boundary force can be calculated easily. Several biomimetic problems are then simulated. Based on the good agreement between the current results and those in the literature, it may be concluded that the present IB-LBM has the capabilitv to handle various biomimetic oroblems.
基金The National Key R&D Program of China under contract No.2019YFD0901404the National Natural Science Foundation of China under contract No.41876141+3 种基金the Program for Professor of Special Appointment(Eastern Scholar)at Shanghai Institutions of Higher Learning under contract No.0810000243the Shanghai Pujiang Program under contract No.18PJ1404100the Shanghai Science and Technology Innovation Action Plan under contract No.19DZ1207502Programs Foundation of Ministry of Education of China under contract No.20133104120001。
文摘The analysis of growth bands in the eyestalk has been increasingly used for estimating crustacean ageing and molting.In this study,we developed an effective method to process and observe the eyestalk microstructure of the swimming crab(Portunus trituberculatus).We found that dark pigmentation as a result of boiling has an influence on the observation of the eyestalk microstructure.Choosing an unboiled eyestalk,this study compared the cross section and longitudinal section,and concluded that the cross section is suitable for the observation of growth increments with 6.1%CV(coefficient of variation),and growth bands are suitable for the observation of the longitudinal section with 5.4%CV.The width of growth increments near the edge of the endocuticle is small,and the width of growth increments of the middle part of the endocuticle is large.Relationship of number of growth bands to molting time was fitted to a linear function with the slope not significantly different from 1,indicating that growth bands are formed associated with molting.Periodicity of growth increment formation was calculated as 3.7 d,however was not verified.Our results provide a new improved technique for identification of crustacean molting and growth.
基金Project supported by the National Natural Science Foundation of China(Nos.11772046 and 81870345)。
文摘In this paper,we use machine learning techniques to form a cancer cell model that displays the growth and promotion of synaptic and electrical signals.Here,such a technique can be applied directly to the spiking neural network of cancer cell synapses.The results show that machine learning techniques for the spiked network of cancer cell synapses have the powerful function of neuron models and potential supervisors for different implementations.The changes in the neural activity of tumor microenvironment caused by synaptic and electrical signals are described.It can be used to cancer cells and tumor training processes of neural networks to reproduce complex spatiotemporal dynamics and to mechanize the association of excitatory synaptic structures which are between tumors and neurons in the brain with complex human health behaviors.
文摘A simple and rapid method using liquid chromatography–mass spectrometry(LC-MS) for the determination of metaxalone in human plasma has been developed and validated.Letrozole was used as the internal standard(IS). The plasma samples were simply treated with acetonitrile which allowed the precipitation of plasma proteins. The chromatographic separation was achieved on a Sapphire C18(2.1 mm × 150 mm, 5 μm, Newark, USA)column using the mobile phase(5 mM ammonium acetate containing 0.01% formic acid:acetonitrile(45:55, v/v)) at a flow rate of 0.3 ml/min. The selected ion monitoring(SIM) in the positive mode was used for the determination of [M + H]+m/z 222.1 and 286.1 for metaxalone and letrozole, respectively. The standard curve obtained was linear(r2≥ 0.99)over the concentration range of 30.24-5040 ng/ml. Meanwhile, no interfering peaks or matrix effect was observed. The method established was simple and successfully applied to a pharmacokinetic study of metaxalone in healthy Chinese volunteers after a single oral dose administration of 800 mg metaxalone. The main pharmacokinetic parameters of metaxalone were as follow: Cmax,(1664 ± 1208) ng/ml and(2063 ± 907) ng/ml;AUC0-36,(13925 ± 6590) ng/ml h and(18620 ± 5717) ng/ml h;t1/2,(13.6 ± 7.7) h and(20.3 ± 7.7) h for the reference and test tablets, respectively. These pharmacokinetic parameters of metaxalone in healthy Chinese volunteers were reported for the first time.
文摘Objective: To evaluate the effectiveness of transvaginal cervical cerclage in singleton pregnancies with cervical incompetence, determine the predictive factors of success and failure, and then compare elective and emergency cerclage. Study Design: This was a retrospective study of 62 patients who underwent cervical cerclage in The First Hospital of Jilin University, China, between May 2015 and January 2018. Successful group was defined as those who delivered live babies and failure group who experienced abortion or stillbirth. Results: Out of 104 patients, 62 met inclusion criteria. In 62 cases, 47 (75.8%) succeeded and 15 (24.2%) failed. In successful group, 21 (44.7%) women delivered pretermly and 26 (55.3%) termly. No severe complications occurred except cervical laceration (2, 3.23%), premature rupture of membranes (13, 20.97%). In 62 cases, 40 (64.5%) have ≤2 previous second-trimester losses and 22 (35.5%) have >2 previous second-trimester losses. No significant differences were found in neonatal outcomes. Analysis revealed that higher postoperative C-reactive protein and presence of premature rupture of membranes were the strongest predictors of cerclage failure. Among 62 cases, 48 (77.4%) were allocated in elective and 14 (22.6%) in emergency cerclage. Pregnancy prolongation was significantly more (P = 0.014) in elective group with no significant differences in premature rupture of membranes, neonatal outcomes (all P > 0.05) except Apgar score at 5 min (P = 0.042). Conclusion: Achieving 75.8% live births proves that transvaginal cervical cerclage is an effective and safe technique in prolonging the gestational age, improving the obstetric outcomes in singleton pregnancies with cervical incompetence under various cerclage indications. Postoperative C-reactive protein and premature rupture of membranes are the predictive factors related to success or failure. Elective cerclage is more effective in prolonging the pregnancy compared to emergency cerclage, no significant differences are seen regarding neonatal outcomes and complications.
文摘Objective:autism spectrum disorder(ASD),a serious disorder that begins early in life,con-tinues throughout the lifespan.Children with ASD who are diagnosed early are more responsive to therapeutic interventions and have less social and language impair-ment than children diagnosed later;however,current di-agnostic measures are mostly applied to children older than one year and lack the appropriate biological markers for early diagnosis of ASD.Using bioinformatic analy-sis,this study explores the molecular information mech-anism of ASD.Method:in this study,we used dataset GSE6575 from Gene Expression Omnibus(GEO)to an-alyze the mRNA expression profile of ASD,including 35 ASD samples and 12 normal control samples looking for different genes and we did enrichment analysis of those genes.We then used the STRING database to construct a protein-protein interaction(PPI)network of differential genes.Finally,Cytoscape plug-in cytoHubba was used to search for hub genes.The diagnostic value of the hub genes was verified by subject operating characteristic curves.Result:we looked for 50 different genes and did an enrichment analysis of those genes.The results of the enrichment analysis showed that these differential genes were mainly concentrated in the response to viruses,the immune regulation of inflammation and energy metabo-lism.Using Cytoscape plug-in cytoHubba,we found ten different genes.We drew ROC curves for all ten genes among which two genes,interleukin 2 receptor subunit beta(IL2Rβ)and perforin 1(PRF1),had good sensitivity and specificity for the early diagnosis of autism.The ar-eas under the ROC curves were 0.855,0.830 for IL2Rβ,PRF1.Conclusion:data analysis using the GEO data-base can provide new insights into the etiology of ASD as well as some possible biomarkers and therapeutic targets for early diagnosis and treatment of ASD.