Biomineralization through microbial process has attracted great attention in the field of geotechnical engineering due to its ability to bind granular materials,clog pores,and seal fractures.Although minerals formed b...Biomineralization through microbial process has attracted great attention in the field of geotechnical engineering due to its ability to bind granular materials,clog pores,and seal fractures.Although minerals formed by biomineralization are generally the same as that by mineralization,their mechanical behaviors show a significant discrepancy.This study aims to figure out the differences between biomineralization and mineralization processes by visualizing and tracking the formation of minerals using microfluidics.Both biomineralization and mineralization processes occurred in the Y-shaped sandcontaining microchip that mimics the underground sand layers.Images from different areas in the reaction microchannel of microchips were captured to directly compare the distribution of minerals.Crystal size and numbers from different reaction times were measured to quantify the differences between biomineralization and mineralization processes in terms of crystal kinetics.Results showed that the crystals were precipitated in a faster and more uncontrollable manner in the mineralization process than that in the biomineralization process,given that those two processes presented similar precipitation stages.In addition,a more heterogeneous distribution of crystals was observed during the biomineralization process.The precipitation behaviors were further explained by the classical nucleation crystal growth theory.The present microfluidic tests could advance the understanding of biomineralization and provide new insight into the optimization of biocementation technology.展开更多
Marine heatwaves(MHWs)can cause irreversible damage to marine ecosystems and livelihoods.Appropriate MHW characterization remains difficult,because the choice of a sea surface temperature(SST)temporal baseline strongl...Marine heatwaves(MHWs)can cause irreversible damage to marine ecosystems and livelihoods.Appropriate MHW characterization remains difficult,because the choice of a sea surface temperature(SST)temporal baseline strongly influences MHW identification.Following a recent work suggesting that there should be a communicating baseline for long-term ocean temperature trends(LTT)and MHWs,we provided an effective and quantitative solution to calculate LTT and MHWs simultaneously by using the ensemble empirical mode decomposition(EEMD)method.The long-term nonlinear trend of SST obtained by EEMD shows superiority over the traditional linear trend in that the data extension does not alter prior results.The MHWs identified from the detrended SST data exhibited low sensitivity to the baseline choice,demonstrating the robustness of our method.We also derived the total heat exposure(THE)by combining LTT and MHWs.The THE was sensitive to the fixed-period baseline choice,with a response to increasing SST that depended on the onset time of a perpetual MHW state(identified MHW days equal to the year length).Subtropical areas,the Indian Ocean,and part of the Southern Ocean were most sensitive to the long-term global warming trend.展开更多
A maximal photon number entangled state,namely NOON state,can be adopted for sensing with a quantum enhancedprecision.In this work,we designed silicon quantum photonic chips containing two types of Mach-Zehnder interf...A maximal photon number entangled state,namely NOON state,can be adopted for sensing with a quantum enhancedprecision.In this work,we designed silicon quantum photonic chips containing two types of Mach-Zehnder interferometerswherein the two-photon NOON state,sensing element for temperature or humidity,is generated.Compared with classicallight or single photon case,two-photon NOON state sensing shows a solid enhancement in the sensing resolution andprecision.As the first demonstration of on-chip quantum photonic sensing,it reveals the advantages of photonic chips forhigh integration density,small-size,stability for multiple-parameter sensing serviceability.A higher sensing precision isexpected to beat the standard quantum limit with a higher photon number NOON state.展开更多
The ORB-SLAM2 based on the constant velocity model is difficult to determine the search window of the reprojection of map points when the objects are in variable velocity motion,which leads to a false matching,with an...The ORB-SLAM2 based on the constant velocity model is difficult to determine the search window of the reprojection of map points when the objects are in variable velocity motion,which leads to a false matching,with an inaccurate pose estimation or failed tracking.To address the challenge above,a new method of feature point matching is proposed in this paper,which combines the variable velocity model with the reverse optical flow method.First,the constant velocity model is extended to a new variable velocity model,and the expanded variable velocity model is used to provide the initial pixel shifting for the reverse optical flow method.Then the search range of feature points is accurately determined according to the results of the reverse optical flow method,thereby improving the accuracy and reliability of feature matching,with strengthened interframe tracking effects.Finally,we tested on TUM data set based on the RGB-D camera.Experimental results show that this method can reduce the probability of tracking failure and improve localization accuracy on SLAM(Simultaneous Localization and Mapping)systems.Compared with the traditional ORB-SLAM2,the test error of this method on each sequence in the TUM data set is significantly reduced,and the root mean square error is only 63.8%of the original system under the optimal condition.展开更多
Increasing evidences show that aberrant subchondral bone remodeling plays an important role in the development of osteoarthritis(OA).However,how subchondral bone formation is activated and the mechanism by which incre...Increasing evidences show that aberrant subchondral bone remodeling plays an important role in the development of osteoarthritis(OA).However,how subchondral bone formation is activated and the mechanism by which increased subchondral bone turnover promotes cartilage degeneration during OA remains unclear.Here,we show that the mechanistic target of rapamycin complex 1(mTORC1)pathway is activated in subchondral bone preosteoblasts(Osterix+)from OA patients and mice.Constitutive activation of mTORC1 in preosteoblasts by deletion of the mTORC1 upstream inhibitor,tuberous sclerosis 1,induced aberrant subchondral bone formation,and sclerosis with little-to-no effects on articular cartilage integrity,but accelerated posttraumatic OA development in mice.In contrast,inhibition of mTORC1 in preosteoblasts by disruption of Raptor(mTORC1-specific component)reduced subchondral bone formation and cartilage degeneration,and attenuated post-traumatic OA in mice.Mechanistically,mTORC1 activation promoted preosteoblast expansion and Cxcl12 secretion,which induced subchondral bone remodeling and cartilage degeneration during OA.A Cxcl12-neutralizing antibody reduced cartilage degeneration and alleviated OA in mice.Altogether,these findings demonstrate that mTORC1 activation in subchondral preosteoblasts is not sufficient to induce OA,but can induce aberrant subchondral bone formation and secrete of Cxcl12 to accelerate disease progression following surgical destabilization of the joint.Pharmaceutical inhibition of the pathway presents a promising therapeutic approach for OA treatment.展开更多
A numerical model for jellyfish Rhopilema esculentum stock enhancement is developed for the first time. The model is based on an operational ocean circulation-surface wave coupled forecasting system for the seas off C...A numerical model for jellyfish Rhopilema esculentum stock enhancement is developed for the first time. The model is based on an operational ocean circulation-surface wave coupled forecasting system for the seas off China and adjacent areas and uses a Lagrangian particle-tracking scheme to track the trajectories of released jellyfish. The Jellyfish are modeled as particles with diel vertical migration and are passively drifted by the current and dispersion due to the sub-grid processes. A comparison between the simulation and survey results demonstrate that the model can capture the primary distribution patterns of the released jellyfish. The model results show that the ocean current and indirect wind impact are the main drivers controlling the jellyfish transport. A connectivity matrix between the release sites and fishing grounds indicates the top of the bay is better than the eastern and western coasts for jellyfish fishing. The matrix also shows that only 45% and 27% of the jellyfish released from Wafangdian(WFD) can enter the fishing ground in 2008 and 2010; thus, the site near WFD is not an advisable location for jellyfish release. A Lagrangian probability density function based on a nine-year tracing experiment validates the results and further provides a "climatology" distribution of the released jellyfish.Several experiments are conducted to examine the sensitivity of the model to random walk schemes and to release conditions. The model requires a random walk but is insensitive to the random walk scheme. The experiments with different habitat depths show that if the jellyfish are fixed on the bottom of the water, most of them will be transported to the center, or even out of the bay, by the bottom circulation.展开更多
Simultaneously improving the efficiency and stability on a large scale is significant for the development of photoelectrochemical(PEC)water splitting systems.Here,we demonstrated a novel design of GaP/GaPN core/shell ...Simultaneously improving the efficiency and stability on a large scale is significant for the development of photoelectrochemical(PEC)water splitting systems.Here,we demonstrated a novel design of GaP/GaPN core/shell nanowire(NW)decorated p-Si photocathode for improved PEC hydrogen production performance compared to that of bare p-Si photocathode.The formation of the p-n junction between p-Si and GaP NW promotes charge separation,and the lower conduction band position of GaPN relative to that of GaP further facilitates the transfer of photogenerated electrons to the electrode surface.In addition,the NW morphology both shortens the carrier collection distance and increases the specific surface area,which result in superior reaction kinetics.Moreover,introduction of N in GaP is beneficial for enhancing the light absorption as well as stability.Our efficient and facile strategy can be applied to other solar energy conversion systems as well.展开更多
Owing to high electrical conductivity and ability to reversibly host a variety of inserted ions,2D metallic molybdenum disulfide(1 T-MoS_(2))has demonstrated promising energy storage performance when used as a superca...Owing to high electrical conductivity and ability to reversibly host a variety of inserted ions,2D metallic molybdenum disulfide(1 T-MoS_(2))has demonstrated promising energy storage performance when used as a supercapacitor electrode.However,its charge storage mechanism is still not fully understood,in particular,how the interlayer spacing of 1 T-MoS_(2)would affect its capacitive performance.In this work,molecular dynamics simulations of 1 T-MoS_(2)with interlayer spacing ranging from 0.615 to 1.615 nm have been performed to investigate the resulting charge storage capacity in ionic liquids.Simulations reveal a camel-like capacitance-potential relation,and MoS_(2)with an interlayer spacing of 1.115 nm has the highest volumetric and gravimetric capacitance of118 F cm^(-3)and 42 F g^(-1),respectively.Although ions in MoS_(2)with an interlayer spacing of 1.115 nm diffuse much faster than with interlayer spacings of 1.365 and 1.615 nm,the MoS_(2)with larger interlayer spacing has a much faster-charging process.Our analyses reveal that the ion number density and its charging speed,as well as ion motion paths,have significant impacts on the charging response.This work helps to understand how the interlayer spacing affects the interlayer ion structures and the capacitive performance of MoS_(2),which is important for revealing the charge storage mechanism and designing MoS_(2)supercapacitor.展开更多
BACKGROUND Insertions in exon 19 in the epidermal growth factor receptor gene(EGFR)is a rarely seen mutation in non-small cell lung cancer.These patients have been effectively treated with sequential EGFR tyrosine kin...BACKGROUND Insertions in exon 19 in the epidermal growth factor receptor gene(EGFR)is a rarely seen mutation in non-small cell lung cancer.These patients have been effectively treated with sequential EGFR tyrosine kinase inhibitors(TKIs).CASE SUMMARY Here,we presented a case of non-small cell lung cancer,stage IIIB,with EGFR exon 19 insertion mutation as detected in the right lower lobe by next-generation sequencing.The patient was sequentially treated with first,second,and thirdgeneration EGFR TKIs after the surgical operation.The overall survival of the patient was 21.3 mo.There was no dynamic analysis of drug resistance mechanisms in targeted therapy.CONCLUSION This case emphasized the importance of following the guidelines.In patients with EGFR mutations,repeated and dynamic next-generation sequencing monitoring is necessary to prescribe a personalized treatment plan.展开更多
The incidence of Inactive ovaries of dairy cows in China is relatively high. There is no complete early warning system for the occurrence of ovarian quiescence in clinical cows. This test provides early warning indica...The incidence of Inactive ovaries of dairy cows in China is relatively high. There is no complete early warning system for the occurrence of ovarian quiescence in clinical cows. This test provides early warning indicators for clinical prediction of ovary cessation in dairy cows. This experiment selected blood samples of dairy cows from 60 to 90 days postpartum in the inactive ovaries group and control group. Differential proteins were selected on the basis of proteomics, three energy indexes: AST, Glu, NEFA. Four reproductive hormones: E2, P4, FSH, LH, and four differentially expressed proteins: IGFBP-2, AHSG, APO-A4, and RBP-4. Key enzyme activities: ALDOB, LDHB, ITIH3, GPX3, SPAM1, PKM2. The ELISA test kit was used to detect the content and activity of the above markers in the test bovine serum. Through correlation analysis, binary logistic regression modeling and ROC analysis, a single indicator early warning technique for APOA4 and ITIH3 was established. The early warning values were APOA4 > 28.825 μg/L and ITIH3 > 195.07 ng/L. A multi-index early warning system based on potential biomarkers of APOA4 + ITIH3 and APOA4 + ITIH3 + E2 was established. The former had an early warning value of: APOA4 > 19.55 μg/I;ITIH3 > 191.14 ng/L;the latter has an early warning value: APOA4 > 47.56 μg/L, ITIH4 > 187.80 ng/L, E2 < 69.63 ng/L.展开更多
Neighborhood gardens serve as sensitive sites for human microbial encounters,with phyllosphere microbes directly impacting our respiratory health.Yet,our understanding remains limited on how factors like season,garden...Neighborhood gardens serve as sensitive sites for human microbial encounters,with phyllosphere microbes directly impacting our respiratory health.Yet,our understanding remains limited on how factors like season,garden age,and land use shape the risk of respiratory diseases(RDs)tied to these garden microbes.Here we examined the microbial communities within the phyllosphere of 72 neighborhood gardens across Shanghai,spanning different seasons(warm and cold),garden ages(old and young),and locales(urban and rural).We found a reduced microbial diversity during the cold season,except for Gammaproteobacteria which exhibited an inverse trend.While land use influenced the microbial composition,urban and rural gardens had strikingly similar microbial profiles.Alarmingly,young gardens in the cold season hosted a substantial proportion of RDs-associated species,pointing towards increased respiratory inflammation risks.In essence,while newer gardens during colder periods show a decline in microbial diversity,they have an increased presence of RDs-associated microbes,potentially escalating respiratory disease prevalence.This underscores the pivotal role the garden age plays in enhancing both urban microbial diversity and respiratory health.展开更多
基金We acknowledge the funding support from the National Natural Science Foundation of China(Grant Nos.51922024 and 52078085)Chongqing Talents Program,China(Grant No.cstc2021ycjhbgzxm0051).
文摘Biomineralization through microbial process has attracted great attention in the field of geotechnical engineering due to its ability to bind granular materials,clog pores,and seal fractures.Although minerals formed by biomineralization are generally the same as that by mineralization,their mechanical behaviors show a significant discrepancy.This study aims to figure out the differences between biomineralization and mineralization processes by visualizing and tracking the formation of minerals using microfluidics.Both biomineralization and mineralization processes occurred in the Y-shaped sandcontaining microchip that mimics the underground sand layers.Images from different areas in the reaction microchannel of microchips were captured to directly compare the distribution of minerals.Crystal size and numbers from different reaction times were measured to quantify the differences between biomineralization and mineralization processes in terms of crystal kinetics.Results showed that the crystals were precipitated in a faster and more uncontrollable manner in the mineralization process than that in the biomineralization process,given that those two processes presented similar precipitation stages.In addition,a more heterogeneous distribution of crystals was observed during the biomineralization process.The precipitation behaviors were further explained by the classical nucleation crystal growth theory.The present microfluidic tests could advance the understanding of biomineralization and provide new insight into the optimization of biocementation technology.
基金Supported by the National Natural Science Foundation of China(Nos.41821004,42276025)the Natural Science Foundation of Shandong Province(No.ZR2021MD027)+1 种基金the National Key Research and Development Program of China(No.2022YFE0140500)the Project of“Development of China-ASEAN blue partnership”started in 2021.
文摘Marine heatwaves(MHWs)can cause irreversible damage to marine ecosystems and livelihoods.Appropriate MHW characterization remains difficult,because the choice of a sea surface temperature(SST)temporal baseline strongly influences MHW identification.Following a recent work suggesting that there should be a communicating baseline for long-term ocean temperature trends(LTT)and MHWs,we provided an effective and quantitative solution to calculate LTT and MHWs simultaneously by using the ensemble empirical mode decomposition(EEMD)method.The long-term nonlinear trend of SST obtained by EEMD shows superiority over the traditional linear trend in that the data extension does not alter prior results.The MHWs identified from the detrended SST data exhibited low sensitivity to the baseline choice,demonstrating the robustness of our method.We also derived the total heat exposure(THE)by combining LTT and MHWs.The THE was sensitive to the fixed-period baseline choice,with a response to increasing SST that depended on the onset time of a perpetual MHW state(identified MHW days equal to the year length).Subtropical areas,the Indian Ocean,and part of the Southern Ocean were most sensitive to the long-term global warming trend.
基金supported by the National Key R&D Program of China(Grant No.2022YFF0712800)Innova-tion Program for Quantum Science and Technology(Grant No.2021ZD0301500).
文摘A maximal photon number entangled state,namely NOON state,can be adopted for sensing with a quantum enhancedprecision.In this work,we designed silicon quantum photonic chips containing two types of Mach-Zehnder interferometerswherein the two-photon NOON state,sensing element for temperature or humidity,is generated.Compared with classicallight or single photon case,two-photon NOON state sensing shows a solid enhancement in the sensing resolution andprecision.As the first demonstration of on-chip quantum photonic sensing,it reveals the advantages of photonic chips forhigh integration density,small-size,stability for multiple-parameter sensing serviceability.A higher sensing precision isexpected to beat the standard quantum limit with a higher photon number NOON state.
基金This work was supported by The National Natural Science Foundation of China under Grant No.61304205 and NO.61502240The Natural Science Foundation of Jiangsu Province under Grant No.BK20191401 and No.BK20201136Postgraduate Research&Practice Innovation Program of Jiangsu Province under Grant No.SJCX21_0364 and No.SJCX21_0363.
文摘The ORB-SLAM2 based on the constant velocity model is difficult to determine the search window of the reprojection of map points when the objects are in variable velocity motion,which leads to a false matching,with an inaccurate pose estimation or failed tracking.To address the challenge above,a new method of feature point matching is proposed in this paper,which combines the variable velocity model with the reverse optical flow method.First,the constant velocity model is extended to a new variable velocity model,and the expanded variable velocity model is used to provide the initial pixel shifting for the reverse optical flow method.Then the search range of feature points is accurately determined according to the results of the reverse optical flow method,thereby improving the accuracy and reliability of feature matching,with strengthened interframe tracking effects.Finally,we tested on TUM data set based on the RGB-D camera.Experimental results show that this method can reduce the probability of tracking failure and improve localization accuracy on SLAM(Simultaneous Localization and Mapping)systems.Compared with the traditional ORB-SLAM2,the test error of this method on each sequence in the TUM data set is significantly reduced,and the root mean square error is only 63.8%of the original system under the optimal condition.
基金supported by grants from National Natural Science Foundation of China (Grant Nos.81371990,81625015,81601945,and 81530070)the Program for Changjiang Scholars and Innovative Research Team in University (IRT_16R37)
文摘Increasing evidences show that aberrant subchondral bone remodeling plays an important role in the development of osteoarthritis(OA).However,how subchondral bone formation is activated and the mechanism by which increased subchondral bone turnover promotes cartilage degeneration during OA remains unclear.Here,we show that the mechanistic target of rapamycin complex 1(mTORC1)pathway is activated in subchondral bone preosteoblasts(Osterix+)from OA patients and mice.Constitutive activation of mTORC1 in preosteoblasts by deletion of the mTORC1 upstream inhibitor,tuberous sclerosis 1,induced aberrant subchondral bone formation,and sclerosis with little-to-no effects on articular cartilage integrity,but accelerated posttraumatic OA development in mice.In contrast,inhibition of mTORC1 in preosteoblasts by disruption of Raptor(mTORC1-specific component)reduced subchondral bone formation and cartilage degeneration,and attenuated post-traumatic OA in mice.Mechanistically,mTORC1 activation promoted preosteoblast expansion and Cxcl12 secretion,which induced subchondral bone remodeling and cartilage degeneration during OA.A Cxcl12-neutralizing antibody reduced cartilage degeneration and alleviated OA in mice.Altogether,these findings demonstrate that mTORC1 activation in subchondral preosteoblasts is not sufficient to induce OA,but can induce aberrant subchondral bone formation and secrete of Cxcl12 to accelerate disease progression following surgical destabilization of the joint.Pharmaceutical inhibition of the pathway presents a promising therapeutic approach for OA treatment.
基金The National Natural Science Foundation of China under contract No.41506044the Scientific and Technological Innovation Project financially supported by Qingdao National Laboratory for Marine Science and Technology of China under contract No.2016ASKJ02+2 种基金the National Basic Research Program(973 Program)of China under contract No.2015CB453303the National Natural Science Foundation of China-Shandong Joint Fund for Marine Science Research Centers under contract No.U1606405the International Cooperation Project of Indo-Pacific Ocean Environment Variation and Air-Sea Interaction under contract No.GASI-03-IPOVAI-05
文摘A numerical model for jellyfish Rhopilema esculentum stock enhancement is developed for the first time. The model is based on an operational ocean circulation-surface wave coupled forecasting system for the seas off China and adjacent areas and uses a Lagrangian particle-tracking scheme to track the trajectories of released jellyfish. The Jellyfish are modeled as particles with diel vertical migration and are passively drifted by the current and dispersion due to the sub-grid processes. A comparison between the simulation and survey results demonstrate that the model can capture the primary distribution patterns of the released jellyfish. The model results show that the ocean current and indirect wind impact are the main drivers controlling the jellyfish transport. A connectivity matrix between the release sites and fishing grounds indicates the top of the bay is better than the eastern and western coasts for jellyfish fishing. The matrix also shows that only 45% and 27% of the jellyfish released from Wafangdian(WFD) can enter the fishing ground in 2008 and 2010; thus, the site near WFD is not an advisable location for jellyfish release. A Lagrangian probability density function based on a nine-year tracing experiment validates the results and further provides a "climatology" distribution of the released jellyfish.Several experiments are conducted to examine the sensitivity of the model to random walk schemes and to release conditions. The model requires a random walk but is insensitive to the random walk scheme. The experiments with different habitat depths show that if the jellyfish are fixed on the bottom of the water, most of them will be transported to the center, or even out of the bay, by the bottom circulation.
基金financial support for this work from the National Natural Science Foundation of China (21422303, 21573049, 21872043, 81602643)Beijing Natural Science Foundation (2142036)+1 种基金Youth Innovation Promotion AssociationSpecial Program of “One Belt One Road” of CAS~~
文摘Simultaneously improving the efficiency and stability on a large scale is significant for the development of photoelectrochemical(PEC)water splitting systems.Here,we demonstrated a novel design of GaP/GaPN core/shell nanowire(NW)decorated p-Si photocathode for improved PEC hydrogen production performance compared to that of bare p-Si photocathode.The formation of the p-n junction between p-Si and GaP NW promotes charge separation,and the lower conduction band position of GaPN relative to that of GaP further facilitates the transfer of photogenerated electrons to the electrode surface.In addition,the NW morphology both shortens the carrier collection distance and increases the specific surface area,which result in superior reaction kinetics.Moreover,introduction of N in GaP is beneficial for enhancing the light absorption as well as stability.Our efficient and facile strategy can be applied to other solar energy conversion systems as well.
基金the financial support from the National Natural Science Foundation of China(51876072)the Hubei Provincial Natural Science Foundation of China(2019CFA002,2020CFA093)+1 种基金Sichuan Science and Technology Program(2019YFG0457)the support from the National Energy Research Scientific Computing Center,a DOE Office of Science User Facility supported by the Office of Science of the U.S.Department of Energy under Contract No.DE-AC0205CH11231
文摘Owing to high electrical conductivity and ability to reversibly host a variety of inserted ions,2D metallic molybdenum disulfide(1 T-MoS_(2))has demonstrated promising energy storage performance when used as a supercapacitor electrode.However,its charge storage mechanism is still not fully understood,in particular,how the interlayer spacing of 1 T-MoS_(2)would affect its capacitive performance.In this work,molecular dynamics simulations of 1 T-MoS_(2)with interlayer spacing ranging from 0.615 to 1.615 nm have been performed to investigate the resulting charge storage capacity in ionic liquids.Simulations reveal a camel-like capacitance-potential relation,and MoS_(2)with an interlayer spacing of 1.115 nm has the highest volumetric and gravimetric capacitance of118 F cm^(-3)and 42 F g^(-1),respectively.Although ions in MoS_(2)with an interlayer spacing of 1.115 nm diffuse much faster than with interlayer spacings of 1.365 and 1.615 nm,the MoS_(2)with larger interlayer spacing has a much faster-charging process.Our analyses reveal that the ion number density and its charging speed,as well as ion motion paths,have significant impacts on the charging response.This work helps to understand how the interlayer spacing affects the interlayer ion structures and the capacitive performance of MoS_(2),which is important for revealing the charge storage mechanism and designing MoS_(2)supercapacitor.
文摘BACKGROUND Insertions in exon 19 in the epidermal growth factor receptor gene(EGFR)is a rarely seen mutation in non-small cell lung cancer.These patients have been effectively treated with sequential EGFR tyrosine kinase inhibitors(TKIs).CASE SUMMARY Here,we presented a case of non-small cell lung cancer,stage IIIB,with EGFR exon 19 insertion mutation as detected in the right lower lobe by next-generation sequencing.The patient was sequentially treated with first,second,and thirdgeneration EGFR TKIs after the surgical operation.The overall survival of the patient was 21.3 mo.There was no dynamic analysis of drug resistance mechanisms in targeted therapy.CONCLUSION This case emphasized the importance of following the guidelines.In patients with EGFR mutations,repeated and dynamic next-generation sequencing monitoring is necessary to prescribe a personalized treatment plan.
文摘The incidence of Inactive ovaries of dairy cows in China is relatively high. There is no complete early warning system for the occurrence of ovarian quiescence in clinical cows. This test provides early warning indicators for clinical prediction of ovary cessation in dairy cows. This experiment selected blood samples of dairy cows from 60 to 90 days postpartum in the inactive ovaries group and control group. Differential proteins were selected on the basis of proteomics, three energy indexes: AST, Glu, NEFA. Four reproductive hormones: E2, P4, FSH, LH, and four differentially expressed proteins: IGFBP-2, AHSG, APO-A4, and RBP-4. Key enzyme activities: ALDOB, LDHB, ITIH3, GPX3, SPAM1, PKM2. The ELISA test kit was used to detect the content and activity of the above markers in the test bovine serum. Through correlation analysis, binary logistic regression modeling and ROC analysis, a single indicator early warning technique for APOA4 and ITIH3 was established. The early warning values were APOA4 > 28.825 μg/L and ITIH3 > 195.07 ng/L. A multi-index early warning system based on potential biomarkers of APOA4 + ITIH3 and APOA4 + ITIH3 + E2 was established. The former had an early warning value of: APOA4 > 19.55 μg/I;ITIH3 > 191.14 ng/L;the latter has an early warning value: APOA4 > 47.56 μg/L, ITIH4 > 187.80 ng/L, E2 < 69.63 ng/L.
基金supported by the Natural Science Foundation of China(Project number:32371843)the Science and Technology Commission of Shanghai Municipality(Project number:22230713300).
文摘Neighborhood gardens serve as sensitive sites for human microbial encounters,with phyllosphere microbes directly impacting our respiratory health.Yet,our understanding remains limited on how factors like season,garden age,and land use shape the risk of respiratory diseases(RDs)tied to these garden microbes.Here we examined the microbial communities within the phyllosphere of 72 neighborhood gardens across Shanghai,spanning different seasons(warm and cold),garden ages(old and young),and locales(urban and rural).We found a reduced microbial diversity during the cold season,except for Gammaproteobacteria which exhibited an inverse trend.While land use influenced the microbial composition,urban and rural gardens had strikingly similar microbial profiles.Alarmingly,young gardens in the cold season hosted a substantial proportion of RDs-associated species,pointing towards increased respiratory inflammation risks.In essence,while newer gardens during colder periods show a decline in microbial diversity,they have an increased presence of RDs-associated microbes,potentially escalating respiratory disease prevalence.This underscores the pivotal role the garden age plays in enhancing both urban microbial diversity and respiratory health.
