Phytochemicalinvestigation onthe whole plants of Ypsilandra yunnanensiswas carried out forthe firsttime andledtothe isolation of five new cholestanol glycosides,ypsiyunnosides A–E(1–5),and one known analogue.Their s...Phytochemicalinvestigation onthe whole plants of Ypsilandra yunnanensiswas carried out forthe firsttime andledtothe isolation of five new cholestanol glycosides,ypsiyunnosides A–E(1–5),and one known analogue.Their structures were determined mainly by detailed spectroscopic analysis,including extensive 1D and 2D NMR,MS and UV,as well as chemical methods.Among them,compound 1 possessed a rare 6/6/6/5/5 fused-rings cholestanol sketelon,which was identified as(23R,25R)-3β,16α,26-triol-16,23-cyclocholest-5,17(20)-dien-22-one.Their induced platelet aggregation activities and cytotoxicities were evaluated.Graphical Abstract Five new cholestanol glycosides,ypsiyunnosides A–E(1–5),were isolated from the whole plants of Ypsilandra yunnanensis.Compound 1 possessed a rare 6/6/6/5/5 fused-rings cholestanol sketelon.Their structures were elucidated by a combination of 1D and 2D NMR,MS and chemical analysis.展开更多
BACKGROUND:Edaravone can alleviate brain injury and improve neurological functions and symptoms. This study aimed to investigate the effect of edaravone on the p38Mitogen-activated protein kinases/Caspase-3 (p38MAPK...BACKGROUND:Edaravone can alleviate brain injury and improve neurological functions and symptoms. This study aimed to investigate the effect of edaravone on the p38Mitogen-activated protein kinases/Caspase-3 (p38MAPK/Caspase-3) pathway after diffuse brain injury (DBI) in rats. METHODS: DBI models were established according to the description of Marmarou's method. A total of 250 rats were divided (random number) into four groups: control group (CG, n=45), model group (MG, n=77), low-dose edaravone group (n=67, dosage 5 mg/kg) and high-dose edaravone group (n=61, dosage 10 mg/kg). After 1,6, 24, 48, and 72 hours after injury, brain tissues were collected. The changes of neuron morphous in the hippocampal region were observed through Nissl staining. The expression levels of phosphorylated p38MAPK and caspase-3 were detected by immunohistochemistry and Western blotting respectively. Learning and memory function were tested with Morris water maze from the 3rd to 7th day after injury. RESULTS: Some neurons had histopathologic changes of necrosis and apoptosis in the model group compared with the control group. The phosphorylated p38MAPK expressions increased at 1,6, 4, and 48 hours (P〈0.05), but no significant difference was observed at 72 hours (0.54±0.19 vs. 0.40±0.14, P〉0.05). Caspase-3 expressions increased at 6, 24, 48, and 72 hours respectively (P〈0.05), but there was no significant difference at 1 hour (0.59±0.29 vs. 0.40±0.17, P〉0.05). From the 3rd to 6th day during the Morris water maze test, the latency to find the platform was significantly prolonged (P〈0.05) and times of rats crossing the platform was decreased on the 7th day (2.28±1.18 vs. 8.20±1.52, P〈0.05). The phosphorylated p38MAPK expressions decreased at 6, 24 and 48 hours respectively in the low dose edaravone group compared with the model group (P〈0.05), whereas no significant difference was seen at 1 hour (1.66±0.80 vs. 1.85±0.86, P〉0.05). Caspase-3 expression decreased at 6, 24, 48, and 72 hours (P〈0.05). The latency to find the platform was significantly shortened (P〈0.05), and times of rats crossing the platform increased (4.17±1.15 vs. 2.28±1.18, P〈0.05). The above mentioned parameters changed more significantly in the high-dose edaravone group than in the low-dose edaravone group. CONCLUSION:Edaravone can alleviate brain tissue damage after DBI, inhibit p38MAP signal activation after early injury, reduce the expression of caspase-3, and promote the recovery of neurological function in the late period.展开更多
Rhizoma Paridis(RP,M®),a traditional Chinese medicine,is the rhizoma of Paris polyphylla var.yunnanensis(PPY)or P.polyphylla var.chinensis which are widely used as important raw materials for several Chinese pate...Rhizoma Paridis(RP,M®),a traditional Chinese medicine,is the rhizoma of Paris polyphylla var.yunnanensis(PPY)or P.polyphylla var.chinensis which are widely used as important raw materials for several Chinese patent drugs.However,the wild resources of these herbs have become less and less due to their slow-growing characteristics and previously excessive excavation.This review covers untiring investigations on alternative resources of RP by our research group over the past decades,including non-medicinal parts of PPY as well as other plants of Liliaceae and Liliflorae families.The arial parts of PPY and the whole plants of Trillium kamtschaticum might be alternative resources for RP based on the fact that they shared the same or similar saponins and bioactivities.展开更多
The whole plants of Ypsilandra thibetica have been analyzed as part of a systematic study on saponin constituents of medicinal plants.This has resulted in the isolation of two new bisdesmosidic furostanol saponins,nam...The whole plants of Ypsilandra thibetica have been analyzed as part of a systematic study on saponin constituents of medicinal plants.This has resulted in the isolation of two new bisdesmosidic furostanol saponins,named ypsilandroside P(1)and ypsilandroside Q(2),and one new pregnane glycoside,named ypsilandroside R(3),together with nine known steroidal glycosides.Their structures were elucidated on the basis of extensive spectroscopic analysis,including that of 2D NMR data,and the results of acidic hydrolysis.Ypsilandroside P(1)was cytotoxicity against two human tumor cell lines.展开更多
Objective:To observe the effect of visual training on cognitive function in stoke patients.Methods:Eighty stroke patients with cognitive dysfunction were divided into two groups(n?40 in each group).The control group r...Objective:To observe the effect of visual training on cognitive function in stoke patients.Methods:Eighty stroke patients with cognitive dysfunction were divided into two groups(n?40 in each group).The control group received conventional rehabilitation therapies.The experimental group received visual training in addition to the conventional therapies.This training was administered for 30 min once a day,five times a week,for four weeks.All patients were screened with the Montreal Cognitive Assessment(MoCA)both before and after the four weeks of intervention.Results:After the four-week intervention,patients in the experimental group increased their scores for attention and concentration,executive function,memory,visual skills,abstract thinking,calculation,and directional force,as well as their total standard score(p<0.01).The patients in the control group also increased their scores in the executive function,visual skills and abstract thinking,as well as their total standard score(p<0.05).However,the experimental group scored higher than the control group for both the individual and total standard scores(p<0.05).Conclusion:Visual training could improve cognitive function of patients with stroke.展开更多
Purpose:To determine the correlation between socioeconomic status and health selfmanagement in the elderly and to provide a scientific basis for the promotion of healthy ageing.Methods:We recruited 6173 people aged60...Purpose:To determine the correlation between socioeconomic status and health selfmanagement in the elderly and to provide a scientific basis for the promotion of healthy ageing.Methods:We recruited 6173 people aged60 years from three regions in Hebei Province using the multi-stage stratified cluster sampling method.The participants were surveyed using questionnaires.Results:The health self-management behaviours of elderly people differed with the area of residence,education level,and personal monthly income.An ordered probit model showed that behaviours were better in elderly people who lived in urban areas,who were educated to college level or higher,and with higher monthly incomes(p<0.05).Conclusion:Socioeconomic status plays a decisive role in health self-management in the elderly.We suggest appropriate measures for promoting health self-management among the elderly.展开更多
基金This work was supported by the National Natural Science Funding of China(Grants 31170333 and 31570363).
文摘Phytochemicalinvestigation onthe whole plants of Ypsilandra yunnanensiswas carried out forthe firsttime andledtothe isolation of five new cholestanol glycosides,ypsiyunnosides A–E(1–5),and one known analogue.Their structures were determined mainly by detailed spectroscopic analysis,including extensive 1D and 2D NMR,MS and UV,as well as chemical methods.Among them,compound 1 possessed a rare 6/6/6/5/5 fused-rings cholestanol sketelon,which was identified as(23R,25R)-3β,16α,26-triol-16,23-cyclocholest-5,17(20)-dien-22-one.Their induced platelet aggregation activities and cytotoxicities were evaluated.Graphical Abstract Five new cholestanol glycosides,ypsiyunnosides A–E(1–5),were isolated from the whole plants of Ypsilandra yunnanensis.Compound 1 possessed a rare 6/6/6/5/5 fused-rings cholestanol sketelon.Their structures were elucidated by a combination of 1D and 2D NMR,MS and chemical analysis.
文摘BACKGROUND:Edaravone can alleviate brain injury and improve neurological functions and symptoms. This study aimed to investigate the effect of edaravone on the p38Mitogen-activated protein kinases/Caspase-3 (p38MAPK/Caspase-3) pathway after diffuse brain injury (DBI) in rats. METHODS: DBI models were established according to the description of Marmarou's method. A total of 250 rats were divided (random number) into four groups: control group (CG, n=45), model group (MG, n=77), low-dose edaravone group (n=67, dosage 5 mg/kg) and high-dose edaravone group (n=61, dosage 10 mg/kg). After 1,6, 24, 48, and 72 hours after injury, brain tissues were collected. The changes of neuron morphous in the hippocampal region were observed through Nissl staining. The expression levels of phosphorylated p38MAPK and caspase-3 were detected by immunohistochemistry and Western blotting respectively. Learning and memory function were tested with Morris water maze from the 3rd to 7th day after injury. RESULTS: Some neurons had histopathologic changes of necrosis and apoptosis in the model group compared with the control group. The phosphorylated p38MAPK expressions increased at 1,6, 4, and 48 hours (P〈0.05), but no significant difference was observed at 72 hours (0.54±0.19 vs. 0.40±0.14, P〉0.05). Caspase-3 expressions increased at 6, 24, 48, and 72 hours respectively (P〈0.05), but there was no significant difference at 1 hour (0.59±0.29 vs. 0.40±0.17, P〉0.05). From the 3rd to 6th day during the Morris water maze test, the latency to find the platform was significantly prolonged (P〈0.05) and times of rats crossing the platform was decreased on the 7th day (2.28±1.18 vs. 8.20±1.52, P〈0.05). The phosphorylated p38MAPK expressions decreased at 6, 24 and 48 hours respectively in the low dose edaravone group compared with the model group (P〈0.05), whereas no significant difference was seen at 1 hour (1.66±0.80 vs. 1.85±0.86, P〉0.05). Caspase-3 expression decreased at 6, 24, 48, and 72 hours (P〈0.05). The latency to find the platform was significantly shortened (P〈0.05), and times of rats crossing the platform increased (4.17±1.15 vs. 2.28±1.18, P〈0.05). The above mentioned parameters changed more significantly in the high-dose edaravone group than in the low-dose edaravone group. CONCLUSION:Edaravone can alleviate brain tissue damage after DBI, inhibit p38MAP signal activation after early injury, reduce the expression of caspase-3, and promote the recovery of neurological function in the late period.
基金supported by the National Natural Science Foundation of China(Nos.31570363,31770391,and 31600283)the Natural Science Foundation of Yunnan Province(2015FA031 and 2017FB128)+2 种基金the Science and Technology Research Program(Grant No.KIB2016001)of Kunming Institute of Botany,CAS,Guiding Program of Interdisciplinary Studies from Kunming Institute of Botany,CAS(Grant No.KIB2017004)the Foundation of State Key Laboratory of Phytochemistry and Plant Resources in West China(P2017-ZZ04)Kunming Institute of Botany,Chinese Academy of Sciences.
文摘Rhizoma Paridis(RP,M®),a traditional Chinese medicine,is the rhizoma of Paris polyphylla var.yunnanensis(PPY)or P.polyphylla var.chinensis which are widely used as important raw materials for several Chinese patent drugs.However,the wild resources of these herbs have become less and less due to their slow-growing characteristics and previously excessive excavation.This review covers untiring investigations on alternative resources of RP by our research group over the past decades,including non-medicinal parts of PPY as well as other plants of Liliaceae and Liliflorae families.The arial parts of PPY and the whole plants of Trillium kamtschaticum might be alternative resources for RP based on the fact that they shared the same or similar saponins and bioactivities.
基金supported by National Natural Science Funding of China(No.31170333)the Natural Sci-ence Foundation of Yunnan Province(No.2009CC019)+1 种基金the West Light Foundation of the Chinese Academy of Sciences(No.2908025712W1)a fund(No.540806321211)of State Key Laboratory of Phytochemistry and Plant Resources in West China,Germplasm Bank of Wild Species and CAS Innovation Program of Kunming Institute of Botany.
文摘The whole plants of Ypsilandra thibetica have been analyzed as part of a systematic study on saponin constituents of medicinal plants.This has resulted in the isolation of two new bisdesmosidic furostanol saponins,named ypsilandroside P(1)and ypsilandroside Q(2),and one new pregnane glycoside,named ypsilandroside R(3),together with nine known steroidal glycosides.Their structures were elucidated on the basis of extensive spectroscopic analysis,including that of 2D NMR data,and the results of acidic hydrolysis.Ypsilandroside P(1)was cytotoxicity against two human tumor cell lines.
基金the Ethics Committee of Hebei United University(approval number 14057/2014),and the patients voluntarily joined this study with informed consents.
文摘Objective:To observe the effect of visual training on cognitive function in stoke patients.Methods:Eighty stroke patients with cognitive dysfunction were divided into two groups(n?40 in each group).The control group received conventional rehabilitation therapies.The experimental group received visual training in addition to the conventional therapies.This training was administered for 30 min once a day,five times a week,for four weeks.All patients were screened with the Montreal Cognitive Assessment(MoCA)both before and after the four weeks of intervention.Results:After the four-week intervention,patients in the experimental group increased their scores for attention and concentration,executive function,memory,visual skills,abstract thinking,calculation,and directional force,as well as their total standard score(p<0.01).The patients in the control group also increased their scores in the executive function,visual skills and abstract thinking,as well as their total standard score(p<0.05).However,the experimental group scored higher than the control group for both the individual and total standard scores(p<0.05).Conclusion:Visual training could improve cognitive function of patients with stroke.
基金supported by the National Social Science Fund Project(ID:12BRK017).
文摘Purpose:To determine the correlation between socioeconomic status and health selfmanagement in the elderly and to provide a scientific basis for the promotion of healthy ageing.Methods:We recruited 6173 people aged60 years from three regions in Hebei Province using the multi-stage stratified cluster sampling method.The participants were surveyed using questionnaires.Results:The health self-management behaviours of elderly people differed with the area of residence,education level,and personal monthly income.An ordered probit model showed that behaviours were better in elderly people who lived in urban areas,who were educated to college level or higher,and with higher monthly incomes(p<0.05).Conclusion:Socioeconomic status plays a decisive role in health self-management in the elderly.We suggest appropriate measures for promoting health self-management among the elderly.