Lithium-sulfur(Li-S)batteries hold great promises to serve as next-generation energy storage devices because of their high theoretical energy density and environmental benignity.However,the shuttle effect of the solub...Lithium-sulfur(Li-S)batteries hold great promises to serve as next-generation energy storage devices because of their high theoretical energy density and environmental benignity.However,the shuttle effect of the soluble lithium polysulfides(LiPS)and intrinsic insulating nature of sulfur lead to low sulfur utilization and coulombic efficiency,leading to poor cycling performance.The impeded charge transportation and retard LiPS catalytic conversion also endows the Li-S batteries with sluggish redox reaction,leading to unsatisfied rate capability.In this study,Co-based MOF material ZIF-67 is used as the precursor to prepare Co nano-dots decorated three-dimensional graphene aerogel as sulfur immobilizer.This porous architecture establishes a highly conductive interconnected framework for fast charge/mass transportation.The exposed Co nano-dots serve as active sites to strongly trap LiPS,which endows CoNDs@G with low decomposition energy barrier for fast LiPS conversion reaction and promote the completely Li2 S catalytic transformation.Li-S cells based on the Co-NDs@G cathode exhibits excellent cyclability and a high capacity retention rate of 91.1%in 100 cycles.This strategy offers a new direction to design sulfur immobilizer for accelerated LiPS conversion kinetics of Li-S batteries.展开更多
The monkeypox virus(MPXV)has triggered a current outbreak globally.Genome sequencing of MPXV and rapid tracing of genetic variants will benefit disease diagnosis and control.It is a significant challenge but necessary...The monkeypox virus(MPXV)has triggered a current outbreak globally.Genome sequencing of MPXV and rapid tracing of genetic variants will benefit disease diagnosis and control.It is a significant challenge but necessary to optimize the strategy and application of rapid full-length genome identification and to track variations of MPXV in clinical specimens with low viral loads,as it is one of the DNA viruses with the largest genome and the most AT-biased,and has a significant number of tandem repeats.Here we evaluated the performance of metagenomic and amplicon sequencing techniques,and three sequencing platforms in MPXV genome sequencing based on multiple clinical specimens of five mpox cases in Chinese mainland.We rapidly identified the full-length genome of MPXV with the assembly of accurate tandem repeats in multiple clinical specimens.Amplicon sequencing enables cost-effective and rapid sequencing of clinical specimens to obtain high-quality MPXV genomes.Third-generation sequencing facilitates the assembly of the terminal tandem repeat regions in the monkeypox virus genome and corrects a common misassembly in published sequences.Besides,several intra-host single nucleotide variations were identified in the first imported mpox case.This study offers an evaluation of various strategies aimed at identifying the complete genome of MPXV in clinical specimens.The findings of this study will significantly enhance the surveillance of MPXV.展开更多
Dear Editor,Mpox(formerly known as monkeypox)is a zoonotic disease caused by infection with the monkeypox virus(MPXV).Mpox cases have been sporadic over the past few decades,with outbreaks occurring in only a limited ...Dear Editor,Mpox(formerly known as monkeypox)is a zoonotic disease caused by infection with the monkeypox virus(MPXV).Mpox cases have been sporadic over the past few decades,with outbreaks occurring in only a limited number of countries,primarily as a result of imported cases(El Eid et al.,2022;Tan and Gao,2022).展开更多
In 2022,a global outbreak of Mpox(formerly monkeypox)occurred in various countries across Europe and America and rapidly spread to more than 100 countries and regions.The World Health Organization declared the outbrea...In 2022,a global outbreak of Mpox(formerly monkeypox)occurred in various countries across Europe and America and rapidly spread to more than 100 countries and regions.The World Health Organization declared the outbreak to be a public health emergency of international concern due to the rapid spread of the Mpox virus.Consequently,nations intensified their efforts to explore treatment strategies aimed at combating the infection and its dissemination.Nevertheless,the available therapeutic options for Mpox virus infection remain limited.So far,only a few numbers of antiviral compounds have been approved by regulatory authorities.Given the high mutability of the Mpox virus,certain mutant strains have shown resistance to existing pharmaceutical interventions.This highlights the urgent need to develop novel antiviral drugs that can combat both drug resistance and the potential threat of bioterrorism.Currently,there is a lack of comprehensive literature on the pathophysiology and treatment of Mpox.To address this issue,we conducted a review covering the physiological and pathological processes of Mpox infection,summarizing the latest progress of anti-Mpox drugs.Our analysis encompasses approved drugs currently employed in clinical settings,as well as newly identified small-molecule compounds and antibody drugs displaying potential antiviral efficacy against Mpox.Furthermore,we have gained valuable insights from the process of Mpox drug development,including strategies for repurposing drugs,the discovery of drug targets driven by artificial intelligence,and preclinical drug development.The purpose of this review is to provide readers with a comprehensive overview of the current knowledge on Mpox.展开更多
Monkeypox virus(MPXV),a DNA virus belonging to the Orthopoxvirus genus,causes a self-limiting zoonotic disease known as mpox.The human monkeypox infection was first recorded in a 9-month-old child in the Republic of C...Monkeypox virus(MPXV),a DNA virus belonging to the Orthopoxvirus genus,causes a self-limiting zoonotic disease known as mpox.The human monkeypox infection was first recorded in a 9-month-old child in the Republic of Congo in the 1970s(Ladnyj et al.,1972).For a long time,mpox was mainly prevalent in the humid forests of Central Africa and parts of West Africa(Kabugae and El Zowalaty,2019).In recent years,MPXV has been spread to other countries through trade and travel.In 2003,a group of rare pets carrying MPXV was exported from Africa to the United States,and an outbreak of animal-to-human transmission occurred(Di Giulio and Eckburg,2004).In 2018,two cases of mpox were confirmed in travelers from Nigeria to the United Kingdom and one case was confirmed in Israel(Vaughan et al.,2018;Erez et al.,2019).Then,in 2019,one case was confirmed in Singapore(Ng et al.,2019).The outbreak of mpox in multiple non-endemic countries in North America and Europe started in May 2022(WHO,2022b).On July 23,2022,the WHO declared MPXV a Public Health Emergency of International Concern(PHEIC)(WHO,2022a).As of March 5,2023,86,309 confirmed mpox cases have been reported from 107 countries/regions worldwide(WHO,2023).In the mainland of China,the first imported case was confirmed by the Chinese Center for Disease Control and Prevention(Zhao et al.,2022),making this the fifth confirmed mpox infection in China.Neglected zoonotic mpox has been restricted in Africa but now it is back in the spotlight worldwide(Tan and Gao,2022).展开更多
Introduction:The severe acute respiratory syndrome coronavirus 2(SARS-CoV-2)has generated 2,431 variants over the course of its global transmission over the past 3 years.To better evaluate the genomic variation of SAR...Introduction:The severe acute respiratory syndrome coronavirus 2(SARS-CoV-2)has generated 2,431 variants over the course of its global transmission over the past 3 years.To better evaluate the genomic variation of SARS-CoV-2 before and after the optimization of coronavirus disease 2019(COVID-19)prevention and control strategies,we analyzed the genetic evolution branch composition and genomic variation of SARS-CoV-2 in both domestic and imported cases in China(the data from Hong Kong and Macao Special Administrative Regions and Taiwan,China were not included)from September 26,2022 to January 29,2023.Methods:Analysis of the number of genome sequences,sampling time,dynamic changes of evolutionary branches,origin,and clinical typing of SARS-CoV-2 variants submitted by 31 provincial-level administrative divisions(PLADs)and Xinjiang Production and Construction Corps(XPCC)was conducted to assess the accuracy and timeliness of SARS-CoV-2 variant surveillance.Results:From September 26,2022 to January 29,2023,20,013 valid genome sequences of domestic cases were reported in China,with 72 evolutionary branches.Additionally,1,978 valid genome sequences of imported cases were reported,with 169 evolutionary branches.The prevalence of the Omicron variants of SARS-CoV-2 in both domestic and imported cases was consistent with that of international epidemic variants.Conclusions:This study provides an overview of the prevalence of Omicron variants of SARS-CoV-2 in China.After optimizing COVID-19 prevention and control strategies,no novel Omicron variants of SARSCoV-2 with altered biological characteristics or public health significance have been identified since December 1,2022.展开更多
Metagenomic next-generation sequencing(mNGs)has been widely applied to identify pathogens associated with infectious diseases.However,limited studies have explored the use of mNGs-based dynamic pathogen monitoring in ...Metagenomic next-generation sequencing(mNGs)has been widely applied to identify pathogens associated with infectious diseases.However,limited studies have explored the use of mNGs-based dynamic pathogen monitoring in intensive care unit patients with severe pneumonia.Here,we present a clinical case of an 86-year-old male patient with severe pneumonia caused by a fungal infection.During the clinical treatment,four mNGS analyses were performed within two consecutive weeks.Various respiratory fungal pathogens,including Candida orthopsilosis,Candida albicans,and Aspergillus fumigatus were detected by mNGS of bronchoalveolar lavage fluid(BALF).Based on conventional pathogen identification and clinical symptoms,the patient was diagnosed with severe pneumonia caused by a fungal infection.The abundance of fungal species decreased gradually in response to antifungal and empirical therapies,and the fungal infections were effectively con-trolled.In summary,our results demonstrated that mNGS could effectively identify pathogens in patients with severe pneumonia.Additionally,dynamic pathogen monitoring based on mNGS could assist in the precise diag-nosis of complex infections and may facilitate rapid induction of the most appropriate therapy.展开更多
Severe acute respiratory syndrome coronavirus 2(SARS-CoV-2),a single-stranded positive RNA virus,exhibits a high rate of genome mutation and recombination(1–2).Recombinant strains of this virus could potentially affe...Severe acute respiratory syndrome coronavirus 2(SARS-CoV-2),a single-stranded positive RNA virus,exhibits a high rate of genome mutation and recombination(1–2).Recombinant strains of this virus could potentially affect their binding ability to the ACE2 receptor,which may in turn diminish the protection offered by vaccines and neutralizing antibodies.Notably,the Delta and Omicron recombinations have been collectively referred to as Deltacron(3).Furthermore,XBB.1.5,a recombinant strain resulting from the combination of the BA.2 sublineages BA.2.10.1 and BA.2.75,has demonstrated enhanced transmissibility(4).As such,it is imperative to monitor the genome recombination of SARS-CoV-2 in order to provide valuable insights regarding epidemic and transmission trends.展开更多
Introduction:Since 2019,numerous variants of concern for severe acute respiratory syndrome virus 2(SARS-CoV-2)have emerged,leading to significant outbreaks.The development of novel,highly accurate,and rapid detection ...Introduction:Since 2019,numerous variants of concern for severe acute respiratory syndrome virus 2(SARS-CoV-2)have emerged,leading to significant outbreaks.The development of novel,highly accurate,and rapid detection techniques for these new SARSCoV-2 variants remains a primary focus in the ongoing efforts to control and prevent the coronavirus disease 2019(COVID-19)pandemic.Methods:Reverse transcription-recombinase polymerase amplification combined with the clustered regularly interspaced short palindromic repeatsassociated protein 12a(CRISPR/Cas12a)system was used to validate the detection of the Omicron BA.2,BA.4,and BA.5 variants of SARS-CoV-2.Results:Our results demonstrate that the CRISPR/Cas12a assay is capable of effectively detecting the SARS-CoV-2 BA.2,BA.4,and BA.5 variants with a limit of detection of 10,1,and 10 copies/μL,respectively.Importantly,our assay successfully differentiated the three SARS-CoV-2 Omicron strains from one another.Additionally,we evaluated 46 SARS-CoV-2 positive clinical samples consisting of BA.2(n=20),BA.4(n=6),and BA.5(n=20)variants,and the sensitivity of our assay ranged from 90%to 100%,while the specificity was 100%.Discussion:This research presents a swift and reliable CRISPR-based method that may be employed to track the emergence of novel SARS-CoV-2 variants.展开更多
Autonomous driving is a promising way to future safe,efficient,and low-carbon transportation.Real-time ac-curate target detection is an essential precondition for the generation of proper following decision and contro...Autonomous driving is a promising way to future safe,efficient,and low-carbon transportation.Real-time ac-curate target detection is an essential precondition for the generation of proper following decision and control signals.However,considering the complex practical scenarios,accurate recognition of occluded targets is a major challenge of target detection for autonomous driving with limited computational capability.To reveal the overlap and difference between various occluded object detection by sharing the same available sensors,this paper presents a review of detection methods for occluded objects in complex real-driving scenarios.Considering the rapid development of autonomous driving technologies,the research analyzed in this study is limited to the recent five years.The study of occluded object detection is divided into three parts,namely occluded vehicles,pedes-trians and traffic signs.This paper provided a detailed summary of the target detection methods used in these three parts according to the differences in detection methods and ideas,which is followed by the comparison of advantages and disadvantages of different detection methods for the same object.Finally,the shortcomings and limitations of the existing detection methods are summarized,and the challenges and future development prospects in this field are discussed.展开更多
Monkeypox is a zoonotic viral disease caused by the monkeypox virus(MPXV),and historically,all outbreaks have been linked to Africa;however,monkeypox has been posing an alarming challenge to the world in 2022(1)as app...Monkeypox is a zoonotic viral disease caused by the monkeypox virus(MPXV),and historically,all outbreaks have been linked to Africa;however,monkeypox has been posing an alarming challenge to the world in 2022(1)as approximately 60,000 cases have been reported in more than 100 nations and regions worldwide(2).Currently,many cases of monkeypox were identified in many nonendemic countries outside of Central and West Africa,and human-to-human transmission has occurred frequently,especially among men who have sex with men(MSM)presenting new clinical symptoms similar to syphilis and other sexually transmitted infections(3).展开更多
On April 27,2022,an international flight KL857 from Amsterdam,the Netherlands arrived at Pudong International Airport,Shanghai Municipality.Passengers were transferred to the quarantine hotel for a routine 14-day medi...On April 27,2022,an international flight KL857 from Amsterdam,the Netherlands arrived at Pudong International Airport,Shanghai Municipality.Passengers were transferred to the quarantine hotel for a routine 14-day medical observation in Songjiang District and regularly tested for severe acute respiratory syndrome coronavirus 2(SARS-CoV-2).One of the passengers(a 37-year-old Chinese male)was reported positive and diagnosed as a mild case on April 29.The case set out from Uganda(flight KL535)on April 25 and transferred at Amsterdam,the Netherlands on April 26 and Seoul,the Republic of Korea(KL857)on April 27.The patient has been vaccinated in four doses against coronavirus disease 2019(COVID-19)(Beijing Institute of Biological Products Co.,Ltd)in China and Uganda.After diagnosis,he was transferred to Shanghai Public Health Clinical Center for treatment.He recovered after treatment and was discharged on May 12.展开更多
Dear Editor,The coronavirus disease 2019(COVID-19)caused by the SARS-CoV-2 coronavirus has become a global pandemic.The SARS-CoV-2 genome has a similarity of 96.2%to that of RaTG13,a bat SARS-CoV-2-related coronavirus...Dear Editor,The coronavirus disease 2019(COVID-19)caused by the SARS-CoV-2 coronavirus has become a global pandemic.The SARS-CoV-2 genome has a similarity of 96.2%to that of RaTG13,a bat SARS-CoV-2-related coronavirus detected in Rhinolophus affinis(Paraskevis et al.,2020;Zhou et al.,2020).The SARS-CoV-2 genome also has 85.5%−92.4%sequence similarity to SARS-CoV-2-related coronaviruses from Malayan pangolins that have been seized in anti-smuggling operations in southern China(Guangdong-Pangolin(GD-Pangolin-CoV)and Guangxi-Pangolin(GX-Pangolin-CoV)genomes)(Liu et al.,2019;Lam et al.,2020).展开更多
An ongoing multi-country outbreak of monkeypox was reported in May 2022 with several deaths,affecting 107 countries of all six World Health Organization(WHO)regions.The WHO has declared the current monkeypox outbreak ...An ongoing multi-country outbreak of monkeypox was reported in May 2022 with several deaths,affecting 107 countries of all six World Health Organization(WHO)regions.The WHO has declared the current monkeypox outbreak to be a Public Health Emergency of International Concern.It is,thus,necessary to rapidly and accurately detect and distinguish different monkeypox virus(MPXV)clades.We designed primers and probes based on the alignment of 138 complete genomes of poxviruses.In Panel 1,we mixed one pair of primers and three probes to detect and differentiate the MPXV Western Africa(IIa,IIb clade)and Congo Basin(I clade)and other orthopoxviruses.In Panel 2,we mixed one pair of primers and two probes to detect the 2022 MPXV(B.1 lineage and its descendant lineages).In addition,we tested the specificity and sensitivity of the assay using real-time PCR.In Panel 1,the assay reproducibly identified various concentrations of two plasmids of the monkeypox virus,whereas other orthopoxviruses did not cross-react.In Panel 2,the probe annealed well to MPXV B.1 and showed the expected linearity.These two multiple real-time assays are inclusive and highly specific for identifying different clades of MPXV.展开更多
On April 29,2022,a flight arrived at Baiyun International Airport,Guangzhou City,Guangdong Province,and departed from Amsterdam Schiphol Airport,Netherlands.After the first test of severe acute respiratory syndrome co...On April 29,2022,a flight arrived at Baiyun International Airport,Guangzhou City,Guangdong Province,and departed from Amsterdam Schiphol Airport,Netherlands.After the first test of severe acute respiratory syndrome coronavirus 2(SARS-CoV-2)nucleic acid at the Baiyun International Airport,all passengers were admitted to a quarantine hotel for a routine 14-day medical observation.On April 30,one of the passengers(a 20-year-old Chinese female)was reported positive for coronavirus disease 2019(COVID-19),and then a nasopharyngeal swab sample was immediately retested on May 1 and reported positive.展开更多
Introduction:The severe acute respiratory syndrome coronavirus 2(SARS-CoV-2)Omicron variant is the dominant circulating strain worldwide.To assess the importation of SARS-CoV-2 variants in the mainland of China during...Introduction:The severe acute respiratory syndrome coronavirus 2(SARS-CoV-2)Omicron variant is the dominant circulating strain worldwide.To assess the importation of SARS-CoV-2 variants in the mainland of China during the Omicron epidemic,the genomic surveillance data of SARS-CoV-2 from imported coronavirus disease 2019(COVID-19)cases in the mainland of China during the first half of 2022 were analyzed.Methods:Sequences submitted from January to July 2022,with a collection date before June 30,2022,were incorporated.The proportions of SARS-CoV-2 variants as well as the relationships between the origin and destination of each Omicron imported case were analyzed.Results:4,946 sequences of imported cases were submitted from 27 provincial-level administrative divisions(PLADs),and the median submission interval was within 1 month after collection.In 3,851 Omicron sequences with good quality,1 recombinant(XU)and 4 subvariants under monitoring(BA.4,BA.5,BA.2.12.1,and BA.2.13)were recorded,and 3 of them(BA.4,BA.5,and BA.2.12.1)caused local transmissions in the mainland of China later than that recorded in the surveillance.Omicron subvariants dominated in the first half of 2022 and shifted from BA.1 to BA.2 then to BA.4 and BA.5.The percentage of BA.2 in the imported SARS-CoV-2 surveillance data was far higher than that in the Global Initiative on Sharing All Influenza Data(GISAID).The imported cases from Hong Kong Special Administrative Region,China,accounted for 32.30%of Omicron cases sampled,and 98.71%of them were BA.2.Conclusions:The Omicron variant showed the intra-Omicron evolution in the first half of 2022,and all of the Omicron subvariants were introduced into the mainland of China multiple times from multiple different locations.展开更多
The smallest cyclic ammonium salt reported to date, N,N-dimethylpyrrolidinium tetrafluoroborate (P11-BF4), was successively synthesized using a synthesis route without metal ions and halogen ions, then investigated as...The smallest cyclic ammonium salt reported to date, N,N-dimethylpyrrolidinium tetrafluoroborate (P11-BF4), was successively synthesized using a synthesis route without metal ions and halogen ions, then investigated as the electrolyte with Propylene carbonate in EDLCs. The electrochemical characteristics of EDLCs assembled by 1 mol/L P11-BF4/PC paired with activated carbon electrodes were compared to traditional electrolytes. P11-BF4 has proven to have superior voltage resistance by using cyclic voltammetry and constant current charge-discharge testing. Moreover, P11-BF4 exhibits a more brilliant rate performance due to its high conductivity. These results demonstrate that P11-BF4 is an ideal electrolyte to improve the energy density and power density of supercapacitors.展开更多
To facilitate the recovery of Pb/SiO_(2) catalyst,magnetic Pb/Fe_(3)O_(4)/SiO_(2) samples were prepared separately by emulsification,sol-gel and incipient impregnation methods.The catalyst samples were characterized b...To facilitate the recovery of Pb/SiO_(2) catalyst,magnetic Pb/Fe_(3)O_(4)/SiO_(2) samples were prepared separately by emulsification,sol-gel and incipient impregnation methods.The catalyst samples were characterized by means of X-ray diffraction and N_(2) adsorption-desorption,and their catalytic activity was investigated in the reaction for synthesizing propylene carbonate from urea and 1,2-propylene glycol.When the gelatin was applied in the preparation of Fe_(3)O_(4) at 60℃ and the pH value was controlled at 4 in the preparation of Fe_(3)O_(4)/SiO_(2),the Pb/Fe_(3)O_(4)/SiO_(2) sample shows good catalytic activity and magnetism.Under the reaction conditions of a reaction temperature of 180℃,reaction time of 2h,catalyst percentage of 1.7 wt-% and a molar ratio of urea to PG of 1∶4,the yield of propylene carbonate attained was 87.7%.展开更多
基金the financial support from the Natural Science Foundation of Hebei Province(B2020202069)。
文摘Lithium-sulfur(Li-S)batteries hold great promises to serve as next-generation energy storage devices because of their high theoretical energy density and environmental benignity.However,the shuttle effect of the soluble lithium polysulfides(LiPS)and intrinsic insulating nature of sulfur lead to low sulfur utilization and coulombic efficiency,leading to poor cycling performance.The impeded charge transportation and retard LiPS catalytic conversion also endows the Li-S batteries with sluggish redox reaction,leading to unsatisfied rate capability.In this study,Co-based MOF material ZIF-67 is used as the precursor to prepare Co nano-dots decorated three-dimensional graphene aerogel as sulfur immobilizer.This porous architecture establishes a highly conductive interconnected framework for fast charge/mass transportation.The exposed Co nano-dots serve as active sites to strongly trap LiPS,which endows CoNDs@G with low decomposition energy barrier for fast LiPS conversion reaction and promote the completely Li2 S catalytic transformation.Li-S cells based on the Co-NDs@G cathode exhibits excellent cyclability and a high capacity retention rate of 91.1%in 100 cycles.This strategy offers a new direction to design sulfur immobilizer for accelerated LiPS conversion kinetics of Li-S batteries.
基金supported by the National Key Research and Development Program of China(2022YFC2303401,2022YFC2304100,2016YFD0500301,2021YFC0863300)the Beijing Science and Technology Plan(Z211100002521017)the National Natural Science Foundation of China(82241080)。
文摘The monkeypox virus(MPXV)has triggered a current outbreak globally.Genome sequencing of MPXV and rapid tracing of genetic variants will benefit disease diagnosis and control.It is a significant challenge but necessary to optimize the strategy and application of rapid full-length genome identification and to track variations of MPXV in clinical specimens with low viral loads,as it is one of the DNA viruses with the largest genome and the most AT-biased,and has a significant number of tandem repeats.Here we evaluated the performance of metagenomic and amplicon sequencing techniques,and three sequencing platforms in MPXV genome sequencing based on multiple clinical specimens of five mpox cases in Chinese mainland.We rapidly identified the full-length genome of MPXV with the assembly of accurate tandem repeats in multiple clinical specimens.Amplicon sequencing enables cost-effective and rapid sequencing of clinical specimens to obtain high-quality MPXV genomes.Third-generation sequencing facilitates the assembly of the terminal tandem repeat regions in the monkeypox virus genome and corrects a common misassembly in published sequences.Besides,several intra-host single nucleotide variations were identified in the first imported mpox case.This study offers an evaluation of various strategies aimed at identifying the complete genome of MPXV in clinical specimens.The findings of this study will significantly enhance the surveillance of MPXV.
基金supported in part by the National Key Research and Development Program of China (2022YFC2303401, 2021YFA1201003, 2021YFC2301605, 2022YFC2304100, 2022YFC2304101, 2022YFC0869900)the National Natural Science Foundation of China (82241066)。
文摘Dear Editor,Mpox(formerly known as monkeypox)is a zoonotic disease caused by infection with the monkeypox virus(MPXV).Mpox cases have been sporadic over the past few decades,with outbreaks occurring in only a limited number of countries,primarily as a result of imported cases(El Eid et al.,2022;Tan and Gao,2022).
基金supported by National Natural Science Foundation of China(82002192)Natural Science Foundation of Hubei Province(2022CFB539,2022CFD107)+2 种基金Young and middle-aged Talents Project of Hubei Provincial Education Department(Q20222605)Scientific Research Ability Cultivation Fund of Hubei University of Arts and Science(2021KPGJ06)Science and Technology Plan(in the field of Medical and health care)of Xiangyang(2022YL05B,2022YL12A).
文摘In 2022,a global outbreak of Mpox(formerly monkeypox)occurred in various countries across Europe and America and rapidly spread to more than 100 countries and regions.The World Health Organization declared the outbreak to be a public health emergency of international concern due to the rapid spread of the Mpox virus.Consequently,nations intensified their efforts to explore treatment strategies aimed at combating the infection and its dissemination.Nevertheless,the available therapeutic options for Mpox virus infection remain limited.So far,only a few numbers of antiviral compounds have been approved by regulatory authorities.Given the high mutability of the Mpox virus,certain mutant strains have shown resistance to existing pharmaceutical interventions.This highlights the urgent need to develop novel antiviral drugs that can combat both drug resistance and the potential threat of bioterrorism.Currently,there is a lack of comprehensive literature on the pathophysiology and treatment of Mpox.To address this issue,we conducted a review covering the physiological and pathological processes of Mpox infection,summarizing the latest progress of anti-Mpox drugs.Our analysis encompasses approved drugs currently employed in clinical settings,as well as newly identified small-molecule compounds and antibody drugs displaying potential antiviral efficacy against Mpox.Furthermore,we have gained valuable insights from the process of Mpox drug development,including strategies for repurposing drugs,the discovery of drug targets driven by artificial intelligence,and preclinical drug development.The purpose of this review is to provide readers with a comprehensive overview of the current knowledge on Mpox.
基金supported by funding from the National Key R&D Program of China"Risk Identification of Potential New Pathogens and Development of Broad-spectrum Antibodies"(2022YFC2303401).
文摘Monkeypox virus(MPXV),a DNA virus belonging to the Orthopoxvirus genus,causes a self-limiting zoonotic disease known as mpox.The human monkeypox infection was first recorded in a 9-month-old child in the Republic of Congo in the 1970s(Ladnyj et al.,1972).For a long time,mpox was mainly prevalent in the humid forests of Central Africa and parts of West Africa(Kabugae and El Zowalaty,2019).In recent years,MPXV has been spread to other countries through trade and travel.In 2003,a group of rare pets carrying MPXV was exported from Africa to the United States,and an outbreak of animal-to-human transmission occurred(Di Giulio and Eckburg,2004).In 2018,two cases of mpox were confirmed in travelers from Nigeria to the United Kingdom and one case was confirmed in Israel(Vaughan et al.,2018;Erez et al.,2019).Then,in 2019,one case was confirmed in Singapore(Ng et al.,2019).The outbreak of mpox in multiple non-endemic countries in North America and Europe started in May 2022(WHO,2022b).On July 23,2022,the WHO declared MPXV a Public Health Emergency of International Concern(PHEIC)(WHO,2022a).As of March 5,2023,86,309 confirmed mpox cases have been reported from 107 countries/regions worldwide(WHO,2023).In the mainland of China,the first imported case was confirmed by the Chinese Center for Disease Control and Prevention(Zhao et al.,2022),making this the fifth confirmed mpox infection in China.Neglected zoonotic mpox has been restricted in Africa but now it is back in the spotlight worldwide(Tan and Gao,2022).
文摘Introduction:The severe acute respiratory syndrome coronavirus 2(SARS-CoV-2)has generated 2,431 variants over the course of its global transmission over the past 3 years.To better evaluate the genomic variation of SARS-CoV-2 before and after the optimization of coronavirus disease 2019(COVID-19)prevention and control strategies,we analyzed the genetic evolution branch composition and genomic variation of SARS-CoV-2 in both domestic and imported cases in China(the data from Hong Kong and Macao Special Administrative Regions and Taiwan,China were not included)from September 26,2022 to January 29,2023.Methods:Analysis of the number of genome sequences,sampling time,dynamic changes of evolutionary branches,origin,and clinical typing of SARS-CoV-2 variants submitted by 31 provincial-level administrative divisions(PLADs)and Xinjiang Production and Construction Corps(XPCC)was conducted to assess the accuracy and timeliness of SARS-CoV-2 variant surveillance.Results:From September 26,2022 to January 29,2023,20,013 valid genome sequences of domestic cases were reported in China,with 72 evolutionary branches.Additionally,1,978 valid genome sequences of imported cases were reported,with 169 evolutionary branches.The prevalence of the Omicron variants of SARS-CoV-2 in both domestic and imported cases was consistent with that of international epidemic variants.Conclusions:This study provides an overview of the prevalence of Omicron variants of SARS-CoV-2 in China.After optimizing COVID-19 prevention and control strategies,no novel Omicron variants of SARSCoV-2 with altered biological characteristics or public health significance have been identified since December 1,2022.
基金supported by the National Key Research and Development Program of China(2021YFC2300101).
文摘Metagenomic next-generation sequencing(mNGs)has been widely applied to identify pathogens associated with infectious diseases.However,limited studies have explored the use of mNGs-based dynamic pathogen monitoring in intensive care unit patients with severe pneumonia.Here,we present a clinical case of an 86-year-old male patient with severe pneumonia caused by a fungal infection.During the clinical treatment,four mNGS analyses were performed within two consecutive weeks.Various respiratory fungal pathogens,including Candida orthopsilosis,Candida albicans,and Aspergillus fumigatus were detected by mNGS of bronchoalveolar lavage fluid(BALF).Based on conventional pathogen identification and clinical symptoms,the patient was diagnosed with severe pneumonia caused by a fungal infection.The abundance of fungal species decreased gradually in response to antifungal and empirical therapies,and the fungal infections were effectively con-trolled.In summary,our results demonstrated that mNGS could effectively identify pathogens in patients with severe pneumonia.Additionally,dynamic pathogen monitoring based on mNGS could assist in the precise diag-nosis of complex infections and may facilitate rapid induction of the most appropriate therapy.
基金Supported by National Key R&D Program of China(2022YFC230410,2022 YFC2303401,2021YFC2300101 and 2021YFA 1201003).
文摘Severe acute respiratory syndrome coronavirus 2(SARS-CoV-2),a single-stranded positive RNA virus,exhibits a high rate of genome mutation and recombination(1–2).Recombinant strains of this virus could potentially affect their binding ability to the ACE2 receptor,which may in turn diminish the protection offered by vaccines and neutralizing antibodies.Notably,the Delta and Omicron recombinations have been collectively referred to as Deltacron(3).Furthermore,XBB.1.5,a recombinant strain resulting from the combination of the BA.2 sublineages BA.2.10.1 and BA.2.75,has demonstrated enhanced transmissibility(4).As such,it is imperative to monitor the genome recombination of SARS-CoV-2 in order to provide valuable insights regarding epidemic and transmission trends.
基金Supported by the National Key Research and Development Program of China(2021YFC2300101,2021YFC0863300,2022YFC 2304101,2022YFC2303401).
文摘Introduction:Since 2019,numerous variants of concern for severe acute respiratory syndrome virus 2(SARS-CoV-2)have emerged,leading to significant outbreaks.The development of novel,highly accurate,and rapid detection techniques for these new SARSCoV-2 variants remains a primary focus in the ongoing efforts to control and prevent the coronavirus disease 2019(COVID-19)pandemic.Methods:Reverse transcription-recombinase polymerase amplification combined with the clustered regularly interspaced short palindromic repeatsassociated protein 12a(CRISPR/Cas12a)system was used to validate the detection of the Omicron BA.2,BA.4,and BA.5 variants of SARS-CoV-2.Results:Our results demonstrate that the CRISPR/Cas12a assay is capable of effectively detecting the SARS-CoV-2 BA.2,BA.4,and BA.5 variants with a limit of detection of 10,1,and 10 copies/μL,respectively.Importantly,our assay successfully differentiated the three SARS-CoV-2 Omicron strains from one another.Additionally,we evaluated 46 SARS-CoV-2 positive clinical samples consisting of BA.2(n=20),BA.4(n=6),and BA.5(n=20)variants,and the sensitivity of our assay ranged from 90%to 100%,while the specificity was 100%.Discussion:This research presents a swift and reliable CRISPR-based method that may be employed to track the emergence of novel SARS-CoV-2 variants.
基金supported by the National Key Research and Devel-opment Program of China under Grant No.2022YFE0102700Dr Yuhan Huang is a recipient of the ARC Discovery Early Career Research Award(DE220100552).
文摘Autonomous driving is a promising way to future safe,efficient,and low-carbon transportation.Real-time ac-curate target detection is an essential precondition for the generation of proper following decision and control signals.However,considering the complex practical scenarios,accurate recognition of occluded targets is a major challenge of target detection for autonomous driving with limited computational capability.To reveal the overlap and difference between various occluded object detection by sharing the same available sensors,this paper presents a review of detection methods for occluded objects in complex real-driving scenarios.Considering the rapid development of autonomous driving technologies,the research analyzed in this study is limited to the recent five years.The study of occluded object detection is divided into three parts,namely occluded vehicles,pedes-trians and traffic signs.This paper provided a detailed summary of the target detection methods used in these three parts according to the differences in detection methods and ideas,which is followed by the comparison of advantages and disadvantages of different detection methods for the same object.Finally,the shortcomings and limitations of the existing detection methods are summarized,and the challenges and future development prospects in this field are discussed.
文摘Monkeypox is a zoonotic viral disease caused by the monkeypox virus(MPXV),and historically,all outbreaks have been linked to Africa;however,monkeypox has been posing an alarming challenge to the world in 2022(1)as approximately 60,000 cases have been reported in more than 100 nations and regions worldwide(2).Currently,many cases of monkeypox were identified in many nonendemic countries outside of Central and West Africa,and human-to-human transmission has occurred frequently,especially among men who have sex with men(MSM)presenting new clinical symptoms similar to syphilis and other sexually transmitted infections(3).
基金the National Key Research and Development Program of China(2021YFC0863300)Three-Year Initiative Plan for Strengthening Public Health System Construction in Shanghai(GWV-2).
文摘On April 27,2022,an international flight KL857 from Amsterdam,the Netherlands arrived at Pudong International Airport,Shanghai Municipality.Passengers were transferred to the quarantine hotel for a routine 14-day medical observation in Songjiang District and regularly tested for severe acute respiratory syndrome coronavirus 2(SARS-CoV-2).One of the passengers(a 37-year-old Chinese male)was reported positive and diagnosed as a mild case on April 29.The case set out from Uganda(flight KL535)on April 25 and transferred at Amsterdam,the Netherlands on April 26 and Seoul,the Republic of Korea(KL857)on April 27.The patient has been vaccinated in four doses against coronavirus disease 2019(COVID-19)(Beijing Institute of Biological Products Co.,Ltd)in China and Uganda.After diagnosis,he was transferred to Shanghai Public Health Clinical Center for treatment.He recovered after treatment and was discharged on May 12.
基金Supported by grants from the National Natural Science Foundation of China(U1902201)the CAS Light of West China Program to X.L,and from the National Natural Science Foundation of China(91731301)to J.L.
文摘Dear Editor,The coronavirus disease 2019(COVID-19)caused by the SARS-CoV-2 coronavirus has become a global pandemic.The SARS-CoV-2 genome has a similarity of 96.2%to that of RaTG13,a bat SARS-CoV-2-related coronavirus detected in Rhinolophus affinis(Paraskevis et al.,2020;Zhou et al.,2020).The SARS-CoV-2 genome also has 85.5%−92.4%sequence similarity to SARS-CoV-2-related coronaviruses from Malayan pangolins that have been seized in anti-smuggling operations in southern China(Guangdong-Pangolin(GD-Pangolin-CoV)and Guangxi-Pangolin(GX-Pangolin-CoV)genomes)(Liu et al.,2019;Lam et al.,2020).
基金supported by the National Key Research and Development Program of China(2016YFD0500301,2021YFC0863300).
文摘An ongoing multi-country outbreak of monkeypox was reported in May 2022 with several deaths,affecting 107 countries of all six World Health Organization(WHO)regions.The WHO has declared the current monkeypox outbreak to be a Public Health Emergency of International Concern.It is,thus,necessary to rapidly and accurately detect and distinguish different monkeypox virus(MPXV)clades.We designed primers and probes based on the alignment of 138 complete genomes of poxviruses.In Panel 1,we mixed one pair of primers and three probes to detect and differentiate the MPXV Western Africa(IIa,IIb clade)and Congo Basin(I clade)and other orthopoxviruses.In Panel 2,we mixed one pair of primers and two probes to detect the 2022 MPXV(B.1 lineage and its descendant lineages).In addition,we tested the specificity and sensitivity of the assay using real-time PCR.In Panel 1,the assay reproducibly identified various concentrations of two plasmids of the monkeypox virus,whereas other orthopoxviruses did not cross-react.In Panel 2,the probe annealed well to MPXV B.1 and showed the expected linearity.These two multiple real-time assays are inclusive and highly specific for identifying different clades of MPXV.
文摘On April 29,2022,a flight arrived at Baiyun International Airport,Guangzhou City,Guangdong Province,and departed from Amsterdam Schiphol Airport,Netherlands.After the first test of severe acute respiratory syndrome coronavirus 2(SARS-CoV-2)nucleic acid at the Baiyun International Airport,all passengers were admitted to a quarantine hotel for a routine 14-day medical observation.On April 30,one of the passengers(a 20-year-old Chinese female)was reported positive for coronavirus disease 2019(COVID-19),and then a nasopharyngeal swab sample was immediately retested on May 1 and reported positive.
基金supported by the National Natural Science Foundation of China(91731301 and U1902201)the Ministry of Science and Technology of the People’s Republic of China(2020YFC0847000)the Light of West China Program of the Chinese Academy of Sciences.
文摘Introduction:The severe acute respiratory syndrome coronavirus 2(SARS-CoV-2)Omicron variant is the dominant circulating strain worldwide.To assess the importation of SARS-CoV-2 variants in the mainland of China during the Omicron epidemic,the genomic surveillance data of SARS-CoV-2 from imported coronavirus disease 2019(COVID-19)cases in the mainland of China during the first half of 2022 were analyzed.Methods:Sequences submitted from January to July 2022,with a collection date before June 30,2022,were incorporated.The proportions of SARS-CoV-2 variants as well as the relationships between the origin and destination of each Omicron imported case were analyzed.Results:4,946 sequences of imported cases were submitted from 27 provincial-level administrative divisions(PLADs),and the median submission interval was within 1 month after collection.In 3,851 Omicron sequences with good quality,1 recombinant(XU)and 4 subvariants under monitoring(BA.4,BA.5,BA.2.12.1,and BA.2.13)were recorded,and 3 of them(BA.4,BA.5,and BA.2.12.1)caused local transmissions in the mainland of China later than that recorded in the surveillance.Omicron subvariants dominated in the first half of 2022 and shifted from BA.1 to BA.2 then to BA.4 and BA.5.The percentage of BA.2 in the imported SARS-CoV-2 surveillance data was far higher than that in the Global Initiative on Sharing All Influenza Data(GISAID).The imported cases from Hong Kong Special Administrative Region,China,accounted for 32.30%of Omicron cases sampled,and 98.71%of them were BA.2.Conclusions:The Omicron variant showed the intra-Omicron evolution in the first half of 2022,and all of the Omicron subvariants were introduced into the mainland of China multiple times from multiple different locations.
基金financially supported by the Guangdong Power Grid Co., Ltd. (No. GDKJXM20160000)
文摘The smallest cyclic ammonium salt reported to date, N,N-dimethylpyrrolidinium tetrafluoroborate (P11-BF4), was successively synthesized using a synthesis route without metal ions and halogen ions, then investigated as the electrolyte with Propylene carbonate in EDLCs. The electrochemical characteristics of EDLCs assembled by 1 mol/L P11-BF4/PC paired with activated carbon electrodes were compared to traditional electrolytes. P11-BF4 has proven to have superior voltage resistance by using cyclic voltammetry and constant current charge-discharge testing. Moreover, P11-BF4 exhibits a more brilliant rate performance due to its high conductivity. These results demonstrate that P11-BF4 is an ideal electrolyte to improve the energy density and power density of supercapacitors.
基金This work has been supported by the National Natural Science Foundation of China(Grant No.20576025)the Hebei Provincial Fund for Natural Science(B2007000010).
文摘To facilitate the recovery of Pb/SiO_(2) catalyst,magnetic Pb/Fe_(3)O_(4)/SiO_(2) samples were prepared separately by emulsification,sol-gel and incipient impregnation methods.The catalyst samples were characterized by means of X-ray diffraction and N_(2) adsorption-desorption,and their catalytic activity was investigated in the reaction for synthesizing propylene carbonate from urea and 1,2-propylene glycol.When the gelatin was applied in the preparation of Fe_(3)O_(4) at 60℃ and the pH value was controlled at 4 in the preparation of Fe_(3)O_(4)/SiO_(2),the Pb/Fe_(3)O_(4)/SiO_(2) sample shows good catalytic activity and magnetism.Under the reaction conditions of a reaction temperature of 180℃,reaction time of 2h,catalyst percentage of 1.7 wt-% and a molar ratio of urea to PG of 1∶4,the yield of propylene carbonate attained was 87.7%.