The analysis of circulating tumor cells(CTCs)allows a noninvasive method of“real-time liquid biopsy”from the blood samples of cancer patients for the diagnosis of early-stage cancer,prognosis,and monitoring therapeu...The analysis of circulating tumor cells(CTCs)allows a noninvasive method of“real-time liquid biopsy”from the blood samples of cancer patients for the diagnosis of early-stage cancer,prognosis,and monitoring therapeutic response.In this study,we develop a simple,inexpensive,and reliable method that utilizes a small molecule peptide,the asparagine-glycine-arginine(NGR),as a capture probe for the selective enrichment and isolation of circulating tumor cells(CTCs).The multiscale TiO_(2) nanofibers are obtained by electrospinning and calcination.Bovine serum albumin(BSA)is decorated onto TiO_(2) nanofiber surfaces to inhibit non-target cell adhesion,while NGR peptides are conjugated onto the TiO_(2)-BSA surface through the glutaraldehyde(GA)to specifically capture the target cells.The TiO_(2)-BSA-NGR substrate exhibits a high capture sensitivity and efficiency from the mimical blood samples with PC-3 cancer cells as low as 10 cells/mL.The TiO_(2) nanofiber substrate can be a promising strategy for the capture and enumeration of CTCs in cancer progression monitoring.展开更多
基金This work was financially supported by the National Natural Science Foundation of China(21904135,21575154,21775160)the Natural Science Foundation of Jiangsu Province(BK20180250)+2 种基金the Science Foundation of Jiangxi Province(20192ACB21033)the CAS International Cooperation Key program(121E32KYSB20170025)the Jiangsu Province Six Talent Peaks program and the CAS/SAFEA International Innovation Teams program.
文摘The analysis of circulating tumor cells(CTCs)allows a noninvasive method of“real-time liquid biopsy”from the blood samples of cancer patients for the diagnosis of early-stage cancer,prognosis,and monitoring therapeutic response.In this study,we develop a simple,inexpensive,and reliable method that utilizes a small molecule peptide,the asparagine-glycine-arginine(NGR),as a capture probe for the selective enrichment and isolation of circulating tumor cells(CTCs).The multiscale TiO_(2) nanofibers are obtained by electrospinning and calcination.Bovine serum albumin(BSA)is decorated onto TiO_(2) nanofiber surfaces to inhibit non-target cell adhesion,while NGR peptides are conjugated onto the TiO_(2)-BSA surface through the glutaraldehyde(GA)to specifically capture the target cells.The TiO_(2)-BSA-NGR substrate exhibits a high capture sensitivity and efficiency from the mimical blood samples with PC-3 cancer cells as low as 10 cells/mL.The TiO_(2) nanofiber substrate can be a promising strategy for the capture and enumeration of CTCs in cancer progression monitoring.
