Traditional Chinese medicine(TCM)has been increasingly employed in the last decades in China for both preventing and treating a variety of cancers.3-epi-bufotalin is an active ingredient of TCM“Chanpi”with anti-tumo...Traditional Chinese medicine(TCM)has been increasingly employed in the last decades in China for both preventing and treating a variety of cancers.3-epi-bufotalin is an active ingredient of TCM“Chanpi”with anti-tumor potential.However,the effect and mechanism of 3-epi-bufotalin on colorectal cancers were not well disclosed.The present study demonstrated that 3-epi-bufotalin could reduce viability,trigger apoptosis,and block the cell cycle at the G2/M stage in colorectal cancer cell lines HT29,RKO,and COLO205 in vitro.Moreover,3-epi-bufotalin inhibited the JAK1/STAT3 signaling pathway.These results indicated the anti-proliferation ability of 3-epi-bufotalin in colorectal cancer cells.展开更多
Doxorubicin(DOX)is a widely employed tumor therapy,yet its substantial toxic side effects pose a considerable challenge.Bletilla striata has demonstrated efficacy in preventing and treating these toxic side effects in...Doxorubicin(DOX)is a widely employed tumor therapy,yet its substantial toxic side effects pose a considerable challenge.Bletilla striata has demonstrated efficacy in preventing and treating these toxic side effects in clinical practice,with polysaccharides identified as the principal active component.In the present study,16 fractions of B.striata polysaccharides(BsPs)were extracted using diverse methods,including hot-water extraction(HWE),ultrasonic-assisted extraction(UAE),enzyme-assisted extraction(EAE),dilute acid-water extraction(ACWE),and dilute alkali-water extraction(ALWE).These extractions were subsequently precipitated at final ethanol concentrations of 80%and 95%,respectively.The investigation encompassed yields,total carbohydrate content(TCC),total protein content(TPC),preliminary structural properties,and anti-DOX myocardial cytotoxic activity.Results indicated that the extraction method significantly influenced the physicochemical properties,associated functional properties,and anti-DOX myocardial cytotoxic activity of BsPs.HWE and UAE yielded higher BsPs quantities.The relative molecular weight(RMW)distribution of BsPs differed notably between HWE or UAE,EAE,ACWE,and ALWE.The RMW of primary BsPs obtained from HWE and UAE(1.9×10^(7)-1.7×10^(7) Da)exceeded that from EAE(7.5×10^(3)-2.8×10^(4) Da)and ALWE(5.1×10^(4)-1.7×10^(4) Da),with smaller molecular weights primarily precipitated by higher ethanol concentrations.BsPs were composed of Man and Glu,with partial fractions containing small amounts of Gal or Ara,displaying varying molar ratios.Notably,BsPs from ACWE exhibited the most significant structural differences,lacking 1,4-α-D-Glcp and a triple-helical structure.Furthermore,BsPs obtained from HWE,UAE,and EAE demonstrated heightened anti-DOX myocardial cytotoxic activity compared to other methods.This study underscored the influence of extraction methods on BsPs’structure and myocardial protective activity,offering a foundation for exploring structural diversity and employing specific extraction methods to extract polysaccharides with robust myocardial protective properties efficiently.展开更多
基金This work was supported by the Guizhou Provincial Science and Technology Program(QKHZC[2020]4Y154)Science and Technology Plan of Zunyi(ZSKHSZ[2018]18)+3 种基金Science and Technology Plan of Zunyi(ZSKHHZ[2020]83)the Xinmiao Funding of Zunyi Medical University(QKPTRC[2019]022)City School Joint Fund of Zunyi(ZSKHHZ[2021]277)the Innovation Talent Team of Zunyi(ZSKRC[2019]1).
文摘Traditional Chinese medicine(TCM)has been increasingly employed in the last decades in China for both preventing and treating a variety of cancers.3-epi-bufotalin is an active ingredient of TCM“Chanpi”with anti-tumor potential.However,the effect and mechanism of 3-epi-bufotalin on colorectal cancers were not well disclosed.The present study demonstrated that 3-epi-bufotalin could reduce viability,trigger apoptosis,and block the cell cycle at the G2/M stage in colorectal cancer cell lines HT29,RKO,and COLO205 in vitro.Moreover,3-epi-bufotalin inhibited the JAK1/STAT3 signaling pathway.These results indicated the anti-proliferation ability of 3-epi-bufotalin in colorectal cancer cells.
基金Science and Technology Foundation of Guizhou Province(Grant No.QKHJC-ZK[2023]YB524)Science and Technology Plan Project of Guizhou,China(Grant No.QKPTRC[2019]035)+2 种基金Science and Technology Foundation of Guizhou Province(Grant No.QKH[2019]1346)Science and Technology Department of Zunyi city of Guizhou province of China(Grant No.[2020]7)Undergraduate Training Program for Innovation and Entrepreneurship of Zunyi Medical University(Grant No.S202310661248).
文摘Doxorubicin(DOX)is a widely employed tumor therapy,yet its substantial toxic side effects pose a considerable challenge.Bletilla striata has demonstrated efficacy in preventing and treating these toxic side effects in clinical practice,with polysaccharides identified as the principal active component.In the present study,16 fractions of B.striata polysaccharides(BsPs)were extracted using diverse methods,including hot-water extraction(HWE),ultrasonic-assisted extraction(UAE),enzyme-assisted extraction(EAE),dilute acid-water extraction(ACWE),and dilute alkali-water extraction(ALWE).These extractions were subsequently precipitated at final ethanol concentrations of 80%and 95%,respectively.The investigation encompassed yields,total carbohydrate content(TCC),total protein content(TPC),preliminary structural properties,and anti-DOX myocardial cytotoxic activity.Results indicated that the extraction method significantly influenced the physicochemical properties,associated functional properties,and anti-DOX myocardial cytotoxic activity of BsPs.HWE and UAE yielded higher BsPs quantities.The relative molecular weight(RMW)distribution of BsPs differed notably between HWE or UAE,EAE,ACWE,and ALWE.The RMW of primary BsPs obtained from HWE and UAE(1.9×10^(7)-1.7×10^(7) Da)exceeded that from EAE(7.5×10^(3)-2.8×10^(4) Da)and ALWE(5.1×10^(4)-1.7×10^(4) Da),with smaller molecular weights primarily precipitated by higher ethanol concentrations.BsPs were composed of Man and Glu,with partial fractions containing small amounts of Gal or Ara,displaying varying molar ratios.Notably,BsPs from ACWE exhibited the most significant structural differences,lacking 1,4-α-D-Glcp and a triple-helical structure.Furthermore,BsPs obtained from HWE,UAE,and EAE demonstrated heightened anti-DOX myocardial cytotoxic activity compared to other methods.This study underscored the influence of extraction methods on BsPs’structure and myocardial protective activity,offering a foundation for exploring structural diversity and employing specific extraction methods to extract polysaccharides with robust myocardial protective properties efficiently.