A poor biocompatibility and bioactivity of invasive materials remains major problems for biomaterialbased therapy. In this study, we introduced gelatin scaffolds carrying both bone morphogenetic protein-2(BMP-2) biomi...A poor biocompatibility and bioactivity of invasive materials remains major problems for biomaterialbased therapy. In this study, we introduced gelatin scaffolds carrying both bone morphogenetic protein-2(BMP-2) biomimetic peptide and vascular endothelial growth factor-165(VEGF) that achieved controlled release, cell attachment, proliferation and differentiation. To promote osteogenesis with VEGF, we designed the BMP-2 biomimetic peptide that comprised BMP-2 core sequence oligopeptide(SSVPT), phosphoserine, and synthetic cell adhesion factor(RGDS). In vitro cell experiments, the scaffold was conducive to the adhesion and proliferation of rat bone marrow mesenchymal stem cells(r BMSCs). The micro-CT3 D reconstruction of the rat cranial bone defect model showed that bone regeneration patterns occurred from one side edge towards the center area implanted with the prepared cryogel, and tissue section staining analysis demonstrated that the scaffold with double-growth factor can synergistically accelerate bone regeneration. These findings suggested that the obtained gelatin cryogel could serve as a cell-responsive platform for biomaterial-based nonbearing bone repair.展开更多
基金financially supported by the Scientific Research Start-up Funds of Hexi University(KYQD2022004)the 13th Innovation Program Science and Technology for college students of Hexi University(138)。
基金supported by grants from Science and Technology Program of Guangzhou,China (No. 201804010146)Natural Science Foundation of Guangdong Province,China (No.2016A030313341)+2 种基金Science and Technology Planning Project of Guangdong Province,China (No. 2014B020215001)National Natural Science Foundation of China (No. 51973243)Science and Technology Planning Project of Shenzhen (No. JCYJ20190807155801657)。
文摘A poor biocompatibility and bioactivity of invasive materials remains major problems for biomaterialbased therapy. In this study, we introduced gelatin scaffolds carrying both bone morphogenetic protein-2(BMP-2) biomimetic peptide and vascular endothelial growth factor-165(VEGF) that achieved controlled release, cell attachment, proliferation and differentiation. To promote osteogenesis with VEGF, we designed the BMP-2 biomimetic peptide that comprised BMP-2 core sequence oligopeptide(SSVPT), phosphoserine, and synthetic cell adhesion factor(RGDS). In vitro cell experiments, the scaffold was conducive to the adhesion and proliferation of rat bone marrow mesenchymal stem cells(r BMSCs). The micro-CT3 D reconstruction of the rat cranial bone defect model showed that bone regeneration patterns occurred from one side edge towards the center area implanted with the prepared cryogel, and tissue section staining analysis demonstrated that the scaffold with double-growth factor can synergistically accelerate bone regeneration. These findings suggested that the obtained gelatin cryogel could serve as a cell-responsive platform for biomaterial-based nonbearing bone repair.
