Taking the combustor composite structure of a high-strength diesel engine as the main research object,dedicated tests have been conducted to verify the accuracy of three distinct cylinder gasket pressure simulation mo...Taking the combustor composite structure of a high-strength diesel engine as the main research object,dedicated tests have been conducted to verify the accuracy of three distinct cylinder gasket pressure simulation models.Using the measured cylinder gasket compression rebound curve,a gasket unit has been designed and manufactured.For this unit,the influence of the bolt pretension,cylinder body and cylinder head material on gasket sealing pressure has been investigated systematically in conditions of thermo-mechanical coupling.The results show that the bolt pretension force is one of the most important factors affecting the cylinder gasket sealing pressure.The change of the body material has little effect on this pressure.The cylinder gasket seal pressure decreases progressively with the reduction of the elastic module of the cylinder head material.展开更多
Dendritic cells （DCs） are crucial for the induction and maintenance of tumor-specific immune responses. Studies have shown that tumor-associated DCs are immunosuppressed in some human tumors. However, phenotype and ...Dendritic cells （DCs） are crucial for the induction and maintenance of tumor-specific immune responses. Studies have shown that tumor-associated DCs are immunosuppressed in some human tumors. However, phenotype and function of DCs in retinoblastoma （RB） remain unclear. RB cell supernatant （RBcs） was used to treat DCs in vitro to explore the effect of RB cells on DCs. DCs were generated from peripheral blood mono- nuclear cells of healthy donors. On day 5 of culture, DCs were treated with RBcs for 24 h, and then purified using magnetic beads. The maturation of DCs was induced by TNF-a or LPS. After treatment with RBcs, expression of co-stimulatory molecules CD80 and CD86 was elevated in DCs, accompanied by increased production of IL- 12p70, TNF-a, IL-6, IL-113, and IL-8 but decreased pro- duction of IL-10. RBcs neither inhibited DC maturation nor promoted DC apoptosis. Moreover, RBcs-exposed DCs stimulated allogenetic T cell proliferation and T cell- derived cytokine production. These results indicate that RBcs can improve DCs＇ antigen presenting function and capability to activate T cells, suggesting that RB cells may have an immunostimulatory effect on DCs, and DC- based immunotherapy may be adopted in the treatment of RB.展开更多
基金the Medium and Heavy Duty High Power-Density Clean Energy Power of National 863 Project。
文摘Taking the combustor composite structure of a high-strength diesel engine as the main research object,dedicated tests have been conducted to verify the accuracy of three distinct cylinder gasket pressure simulation models.Using the measured cylinder gasket compression rebound curve,a gasket unit has been designed and manufactured.For this unit,the influence of the bolt pretension,cylinder body and cylinder head material on gasket sealing pressure has been investigated systematically in conditions of thermo-mechanical coupling.The results show that the bolt pretension force is one of the most important factors affecting the cylinder gasket sealing pressure.The change of the body material has little effect on this pressure.The cylinder gasket seal pressure decreases progressively with the reduction of the elastic module of the cylinder head material.
文摘Dendritic cells （DCs） are crucial for the induction and maintenance of tumor-specific immune responses. Studies have shown that tumor-associated DCs are immunosuppressed in some human tumors. However, phenotype and function of DCs in retinoblastoma （RB） remain unclear. RB cell supernatant （RBcs） was used to treat DCs in vitro to explore the effect of RB cells on DCs. DCs were generated from peripheral blood mono- nuclear cells of healthy donors. On day 5 of culture, DCs were treated with RBcs for 24 h, and then purified using magnetic beads. The maturation of DCs was induced by TNF-a or LPS. After treatment with RBcs, expression of co-stimulatory molecules CD80 and CD86 was elevated in DCs, accompanied by increased production of IL- 12p70, TNF-a, IL-6, IL-113, and IL-8 but decreased pro- duction of IL-10. RBcs neither inhibited DC maturation nor promoted DC apoptosis. Moreover, RBcs-exposed DCs stimulated allogenetic T cell proliferation and T cell- derived cytokine production. These results indicate that RBcs can improve DCs＇ antigen presenting function and capability to activate T cells, suggesting that RB cells may have an immunostimulatory effect on DCs, and DC- based immunotherapy may be adopted in the treatment of RB.
