Poly(ethylene oxide)(PEO)and Li_(6.75)La_(3)Zr_(1.75)Ta_(0.25)O_(12)(LLZTO)-based composite polymer electrolytes(CPEs)are considered one of the most promising solid electrolyte systems.However,agglomeration of LLZTO w...Poly(ethylene oxide)(PEO)and Li_(6.75)La_(3)Zr_(1.75)Ta_(0.25)O_(12)(LLZTO)-based composite polymer electrolytes(CPEs)are considered one of the most promising solid electrolyte systems.However,agglomeration of LLZTO within PEO and lack of Li^(+)channels result in poor electrochemical properties.Herein,a functional supramolecular combination(CD-TFSI)consisting of activeβ-cyclodextrin(CD)supramolecular with self-assembled LiTFSI salt is selected as an interface modifier to coat LLZTO fillers.Benefiting from vast H-bonds formed betweenβ-CD and PEO matrix and/or LLZTO,homogeneous dispersion and tight interface contact are obtained.Moreover,^(6)Li NMR spectra confirm a new Li^(+)transmission pathway from PEO matrix to LLZTO ceramic then to PEO matrix in the as-prepared PEO/LLZTO@CD-TFSI CPEs due to the typical cavity structure ofβ-CD.As a proof,the conductivity is increased from 5.3×10^(-4)S cm^(-1)to 8.7×10^(-4)S cm^(-1)at 60℃,the Li^(+)transference number is enhanced from 0.38 to 0.48,and the electrochemical stability window is extended to 5.1 V versus Li/Li^(+).Li‖LiFePO_(4)CR2032 coin full cells and pouch cells prove the practical application of the as-prepared PEO/LLZTO@CD-TFSI CPEs.This work offers a new strategy of interface modifying LLZTO fillers with functional supramolecular combination to optimize PEO/LLZTO CPEs for solid lithium batteries.展开更多
为建立鸡新城疫病毒La Sota株在LMH细胞上的全悬浮培养工艺,以获得高滴度的新城疫疫苗抗原,采用LMH贴壁细胞悬浮培养驯化方法,获得了形态良好、能稳定传代的LMH悬浮细胞株;以LMH悬浮细胞在摇瓶培养NDV La Sota株为基础,对接毒细胞密度...为建立鸡新城疫病毒La Sota株在LMH细胞上的全悬浮培养工艺,以获得高滴度的新城疫疫苗抗原,采用LMH贴壁细胞悬浮培养驯化方法,获得了形态良好、能稳定传代的LMH悬浮细胞株;以LMH悬浮细胞在摇瓶培养NDV La Sota株为基础,对接毒细胞密度、接毒剂量、胰酶添加浓度、收获时间等工艺参数进行了摸索和优化,并在14 L生物反应器中进一步进行3个批次的培养验证。结果显示:LMH悬浮细胞以初始密度1.5×10^(6)/mL左右接种,培养72 h可增殖到8.0×10^(6)~9.0×10^(6)/mL,细胞活率达97%以上。确定NDV La Sota株在LMH悬浮细胞株上的培养工艺:LMH细胞悬浮培养至不低于4.5×10^(6)/mL按照感染复数不低于0.2接种病毒,并添加终浓度为5μg/mL的胰酶,于37℃培养72 h左右,细胞活率在30%左右收获病毒液,NDV HA均可达1∶2048~1∶4096,病毒含量≥10^(7.63)EID50/0.1 mL。结果表明成功建立了LMH细胞全悬浮培养工艺,并能在生物反应器扩大培养,为ND相关疫苗研发提供了技术支撑。展开更多
The digital economy has become an important driver for stimulating economic growth.The digital economy has now widely penetrated the fields of economy and society,providing new opportunities for the develop‐ment of u...The digital economy has become an important driver for stimulating economic growth.The digital economy has now widely penetrated the fields of economy and society,providing new opportunities for the develop‐ment of urban-rural integration.Based on panel data for 30 provinces in China from 2011 to 2020,this study constructed an index system for the integration of the digital economy and the development of urban-rural areas and conducted a systematic measurement analysis.Additionally,we used a two-step system of GMM estimation to analyze the impact of the digital economy on the development of urban-rural integra‐tion.The findings demonstrate the significant imbalance paradox of China’s digital economy development,which is shown in a gradient where the eastern region is higher than the center and the central region is higher than the west.Urban-rural integration levels in China fluctuate and display geographical variance,typically displaying high levels in the east and low levels in the west.Urban-rural integration is significantly encouraged by the digital economy,yet it varies in variability between different areas and dimensions.Addi‐tionally,rural human capital moderates the favorable effects of the digital economy on urban-rural integra‐tion.As a result,in order to achieve the integrated development of urban and rural areas,it is imperative to fully exploit the active role of the digital economy,better support the development of rural revitalization,bridge the“digital divide”between urban and rural development,and build a strong foundation for the for‐mation of a digital urban-rural integrated development pattern with urban and rural areas and common con‐struction and sharing.展开更多
基金the financial support of the National Natural Science Foundation of China(Nos.21773167,51972220)the National Key Research and Development Program of China(No.2021YFE0107200)+1 种基金the Natural Science Foundation of the Jiangsu Higher Education Institutions of China(No.20KJA480003)the Key R&D Project funded by Department of Science and Technology of Jiangsu Province(No.BE2020003)
文摘Poly(ethylene oxide)(PEO)and Li_(6.75)La_(3)Zr_(1.75)Ta_(0.25)O_(12)(LLZTO)-based composite polymer electrolytes(CPEs)are considered one of the most promising solid electrolyte systems.However,agglomeration of LLZTO within PEO and lack of Li^(+)channels result in poor electrochemical properties.Herein,a functional supramolecular combination(CD-TFSI)consisting of activeβ-cyclodextrin(CD)supramolecular with self-assembled LiTFSI salt is selected as an interface modifier to coat LLZTO fillers.Benefiting from vast H-bonds formed betweenβ-CD and PEO matrix and/or LLZTO,homogeneous dispersion and tight interface contact are obtained.Moreover,^(6)Li NMR spectra confirm a new Li^(+)transmission pathway from PEO matrix to LLZTO ceramic then to PEO matrix in the as-prepared PEO/LLZTO@CD-TFSI CPEs due to the typical cavity structure ofβ-CD.As a proof,the conductivity is increased from 5.3×10^(-4)S cm^(-1)to 8.7×10^(-4)S cm^(-1)at 60℃,the Li^(+)transference number is enhanced from 0.38 to 0.48,and the electrochemical stability window is extended to 5.1 V versus Li/Li^(+).Li‖LiFePO_(4)CR2032 coin full cells and pouch cells prove the practical application of the as-prepared PEO/LLZTO@CD-TFSI CPEs.This work offers a new strategy of interface modifying LLZTO fillers with functional supramolecular combination to optimize PEO/LLZTO CPEs for solid lithium batteries.
基金Ministry of Education Humanities and Social Science Foundation Youth Project“Micro-quantification,Action Mechanism and Impact Research on Financialization of Entity Enterprises”[Grant number.19YJC790106]National Social Science Fund“Mechanism Analysis and Optimization Path Research of Digital Finance Supporting the Im‐provement of Development Efficiency of SMEs”[Grant number.21BJY047]+2 种基金Science and Technology Research Program of Chongqing Education Commission of China:“Optimization Path Research of Or‐ganizational Effectiveness of SOEs in Chongqing Based on Multi-Level Organizational Citizenship Behavior”[Grant number.17SKG036]Chongqing Social Science Planning Major Project“Research on the Technological Progress Path and Countermeasure System of Innovation-driven Manufacturing Upgrade in Chongqing”[Grant num‐ber.2020ZDJJ01]Chongqing Municipal Education Commission Hu‐manities and Social Sciences Research Project“Western Region Finan‐cial Development and Manufacturing Traditional Comparative Advan‐tage Transformation:Efficiency Measurement,Action Mechanism and Research on Spatial Effects”[Grant number.20SKGH040].
文摘The digital economy has become an important driver for stimulating economic growth.The digital economy has now widely penetrated the fields of economy and society,providing new opportunities for the develop‐ment of urban-rural integration.Based on panel data for 30 provinces in China from 2011 to 2020,this study constructed an index system for the integration of the digital economy and the development of urban-rural areas and conducted a systematic measurement analysis.Additionally,we used a two-step system of GMM estimation to analyze the impact of the digital economy on the development of urban-rural integra‐tion.The findings demonstrate the significant imbalance paradox of China’s digital economy development,which is shown in a gradient where the eastern region is higher than the center and the central region is higher than the west.Urban-rural integration levels in China fluctuate and display geographical variance,typically displaying high levels in the east and low levels in the west.Urban-rural integration is significantly encouraged by the digital economy,yet it varies in variability between different areas and dimensions.Addi‐tionally,rural human capital moderates the favorable effects of the digital economy on urban-rural integra‐tion.As a result,in order to achieve the integrated development of urban and rural areas,it is imperative to fully exploit the active role of the digital economy,better support the development of rural revitalization,bridge the“digital divide”between urban and rural development,and build a strong foundation for the for‐mation of a digital urban-rural integrated development pattern with urban and rural areas and common con‐struction and sharing.
