Efficacy and safety of ulinastatin in the treatment of septic shock:a systematic review and meta-analysis

Background:Septic shock is a common systemic inflammatory response syndrome for critical patients in the intensive care unit.Ulinastatin is currently used for the treatment of septic shock.Our study sought to evaluate the efficacy and safety of ulinastatin in the treatment of septic shock patients.Methods:Three English databases(Embase,Medline,and Cochrane Library)and four Chinese databases(China National Knowledge Infrastructure,Wanfang data,SinoMed,and VIP)were searched for published randomized controlled trials.Stata 16.0 software was used to conduct the meta-analysis.Results:A total of 48 articles were included(Chinese article 47,1 in English).The results show that the treatment of ulinastatin could reduce mortality(risk ratio=0.63,95%confidence interval(CI)(0.55,0.72)),multiple organ dysfunction syndrome(risk ratio=0.6,95%CI(0.53,0.68)),length of intensive care unit stay(mean difference(MD)=-3.92,95%CI(-4.65,-3.18)),length of hospital stay(MD=-4.39,95%CI(-6.63,-2.15))and decrease Acute Physiology and Chronic Health Evaluation II score(MD=-4.55,95%CI(-5.63,-3.47))and Sequential Organ Failure Assessment score(MD=-2.02,95%CI(-2.59,-1.44))with P<0.001.Moreover,it lowers TNF-α(standardized mean difference(SMD)=-1.78,95%CI(-2.24,-1.32)),Interleukin-6(SMD=-1.17,95%CI(-1.55,-0.8)),C reactive protein(SMD=-1.49,95%CI(-1.99,-0.99)),hypersensitive C-reactive protein(SMD=-1.9,95%CI(-2.87,-0.94))and procalcitonin(SMD=-0.89,95%CI(-1.12,-0.67))levels in the body.Conclusions:Available evidence shows that ulinastatin reduces case mortality rate,multiple organ dysfunction syndrome,length of intensive care unit stay,and length of hospital stay and decreases Acute Physiology and Chronic Health Evaluation II score and Sequential Organ Failure Assessment score.Moreover,it also lowers TNF-α,Interleukin-6,C reactive protein,hypersensitive C-reactive protein,and procalcitonin levels in the body.

探究农村金融与乡村振兴的关系

基于2010-2020年30个省域面板数据,运用熵值法构建农村金融与乡村振兴指标体系。采用面板向量自回归(VAR)模型、格兰杰(Granger)因果检验、脉冲响应函数和方差分解等方法,探究农村金融与乡村振兴的相互关系及其动态变化特征。实证研究表明:农村金融与乡村振兴存在高度融合发展;两个体系之间存在较强的双向因果关系。

具有套管结构NaYF4:Yb^3+,Tm^3+@NaGdF4:Ce^3+,Eu^3+晶体的制备及在双模式荧光防伪中的应用

通过聚丙烯酸(PAA)调介的水热法在NaYF4:Yb^3+,Tm^3+微管上外延生长NaGdF4:Ce^3+,Eu^3+壳层,制备新型亲水性NaYF4:Yb^3+,Tm^3+@NaGdF4:Ce^3+,Eu^3+双模式荧光套管(DJMT)。研究表明,PAA配体在NaYF4:Yb^3+,Tm^3+微管表面形成NaGdF4:Ce^3+,Eu^3+壳层中起到重要的结构导向作用。NaYF4:Yb^3+,Tm^3+@NaGdF4:Ce^3+,Eu^3+双模式荧光双夹层微管的生长经历由β-NaGdF4和β-NaYF4两相混合物到β-NaYF4@NaGdF4复合晶体的晶相转变过程,同时,颗粒形貌由β-NaGdF4:Ce^3+,Eu^3+纳米棒与β-NaYF4:Yb^3+,Tm^3+微管的混合物演变为NaYF4:Yb^3+,Tm^3+@NaGdF4:Ce^3+,Eu^3+套管结构。DJMTs的生长机理遵循Ostwald熟化机制,生长过程中原位生成的β-NaGdF4:Ce^3+,Eu^3+纳米棒经过溶解−再成核以及表面沉积等过程,在NaYF4:Yb^3+,Tm^3+微管的表面附着β-NaGdF4:Ce^3+,Eu^3+壳层。制备的DJMT具有蓝色上转换和红色下转换荧光特性,可制成环保的荧光油墨,通过喷墨打印实现高度安全的荧光防伪图案的输出。

A new species of Triplophysa(Cypriniformes,Nemacheilidae) from Weihe River in Gansu Province, China

DEAR EDITOR,A new species of Tibetan loach,Triplophysa weiheensis sp.nov.,is described from the Weihe River in Gansu Province,China,based on morphological and molecular analyses.The new species can be distinguished from all known congeners by a unique combination of the following characters:scaleless;snout abruptly sloping downward,anterior to anterior nostril;lower jaw crescentic,not sharp;body without obvious mottling;lateral line interrupted on posterior trunk at pelvic-fin distal extremity.

子午线轮胎成型机胎体接取传送装置轻量化设计研究

针对子午线轮胎成型机胎体接取传送装置存在的不足,从轻量化结构角度,提出了导柱和夹持爪组件的轻量化设计方案。应用Netfabb软件,对二者进行轻量化设计分析。分析结果表明,导柱和夹持爪组件的轻量化设计方案均可行,导柱和夹持爪组件分别减重51.9%和30.2%。

优化学科布局,推动医学科学创新发展

近年来,国家自然科学基金委员会(简称“基金委”)全面贯彻落实党中央、国务院关于加强基础研究的决策部署,以“构建理念先进、制度规范、公正高效的新时代科学基金体系”为目标,将优化学科布局作为科学基金深化改革的三大核心任务之一.学科布局是促进科技发展的“软基础设施”,承担着重要的使命.

Roles of MAP kinase signaling pathway in oocyte meiosis

Mitogen-activated protein kinase (MAPK) is a family of Ser/Thr protein kinases expressed widely in eu-karyotic cells. MAPK is activated by a cascade of protein kinase phosphorylation and plays pivotal roles in regulating meiosis process in oocytes. As an important physical substrate of MAPK, p90rsk mediates numerous MAPK functions. MAPK was activated at G2/M transition during meiosis. Its activity reached the peak at M I stage and maintained at this level until the time before the pronuclear formation after fertilization. There is complex interplay between MAPK and MPF in the meiosis regulation. Furthermore, other intracel-lular signal transducers, such as cAMP, protein kinase C and protein phosphotase, ect., also regulated the activity of MAPK at different stages during meiosis in oocytes. In the present article, the roles of MAPK signaling pathway in oo-cyte meiosis are reviewed and discussed.

CircRNA expression profiles in decidual tissue of patients with early recurrent miscarriage

Circular RNAs(circRNAs)are a novel class of endogenous noncoding RNAs that play important roles in gene expression regulation.This study aimed to evaluate the potential role of circRNAs in decidual tissue of patients with early recurrent miscarriage(RM).We constructed circRNA expression profiles in decidual tissue using microarray data.A total of 123 differentially expressed circRNAs,including 78 upregulated and 45 downregulated circRNAs were detected in the early RM group compared with the control group(P<0.05).Gene Ontology(GO)and Kyoto Encyclopedia of Genes and Genomes(KEGG)pathway analysis also revealed the enrichment of specific circRNAs.The verified circRNA-targeted miRNA-mRNA network was constructed,most of the circRNAs harbored miRNA binding sites.The network involved 3 circRNAs,27 microRNAs and 82 mRNAs.Hsa_circRNA_103092-miR-224-PRLR network was selected to verify by qRT-PCR.These results showed that circRNAs are aberrantly expressed in the decidual tissue in early RM patients and play potential roles in the development of early RM.It gives new insights into the mechanism of recurrent miscarriage.

The metabolic role of LncZBTB39-1:2 in the trophoblast mobility of preeclampsia

Preeclampsia is characterized by new onset of hypertension and proteinuria after 20 weeks’gestation and is a leading cause of maternal and neonatal morbidity and mortality.The pathogenesis of preeclampsia is often associated with aberrant trophoblast function that leads to shallow placental implantation.However,the exact underlying mechanisms remain unclear.Placental LncZBTB39-1:2 expression level was investigated in 20 healthy placentae and 20 placentae with preeclampsia using qRT-PCR,and the metabolic profile of trophoblasts overexpressing LncZBTB39-1:2 in vitro was analysed using gas chromatography-mass spectrometry(GCeMS).In this study,we found that the expression of LncZBTB39-1:2 was significantly higher in preeclamptic placentae than in healthy placentae.Our metabolomics results have shown that tricarboxylic acid cycle intermediates and metabolites related to carbohydrate metabolism were decreased with the overexpression of LncZBTB39-1:2 in HTR8/SVneo cells.These findings were validated by detecting a lower level of intracellular ATP in HTR8/Vneo cells.Furthermore,the migration of HTR8/SVneo cells was compromised when cells were transfected with a plasmid encompassing LncZBTB39-1:2 overexpression.From these results,we conclude that abnormal levels of LncZBTB39-1:2 expression might lead to aberrant conditions in HTR-8/SVneo trophoblast cells.Aberrant conditions might be associated with dysregulated trophoblast migration and subsequent failure of uterine spiral artery remodelling,a pathogenesis recognised as a contributing factor in the aetiology of preeclampsia.

Dynamin Regulates Autophagy by Modulating Lysosomal Function

Autophagy is a central lysosomal degradation pathway required for maintaining cellular homeostasis and its dysfunction is associated with numerous human diseases. To identify players in autophagy, we tested ~ 1200 chemically induced mutations on the X chromosome in Drosophila fat body clones and discovered that shibire （shi） plays an essential role in starvation-induced autophagy, shi encodes a dynamin protein required for fission of clathrin-coated vesicles from the plasma membrane during endocytosis. We showed that Shi is dispensable for autophagy initiation and autophagosome--lysosome fusion, but required for lysosomal/autolysosomal acidification. We also showed that other endocytic core machinery components like clathrin and AP2 play similar but not identical roles in regulating autophagy and lysosomal function as dynamin. Previous studies suggested that dynamin directly regulates autophagosome formation and autophagic lysosome reformation （ALR） through its excision activity, Here, we provide evidence that dynamin also regulates autophagy indirectly by regulating lysosomal function.

Fragmentation for selection: how the deleterious mt DNA is removed in the female germline

Mitochondria are the energy factories and metabolic centers of cells,and their malfunction can lead to multiple human diseases.As highly dynamic organelles,mitochondria undergo frequent fission and fusion processes[1].The fusion process enables the exchange of inner materials between individual mitochondrion.The materials from healthy mitochondria could potentially compensate for the defects found in the defective mitochondria.Fission processes not only increase the mitochondrial number but also separate the damaged parts of mitochondria from the healthy ones,thus,facilitating the elimination of the defective mitochondria through a process known as mitophagy.Excessive fusion leads to extra connections and branching of the mitochondria,whereas extra fission results in fragmentation of mitochondria.The fusion and fission processes are differently tuned in various tissues to adapt to different physiological requirements[2].