Deep learning-assisted common temperature measurement based on visible light imaging

Real-time,contact-free temperature monitoring of low to medium range(30℃-150℃)has been extensively used in industry and agriculture,which is usually realized by costly infrared temperature detection methods.This paper proposes an alternative approach of extracting temperature information in real time from the visible light images of the monitoring target using a convolutional neural network(CNN).A mean-square error of<1.119℃was reached in the temperature measurements of low to medium range using the CNN and the visible light images.Imaging angle and imaging distance do not affect the temperature detection using visible optical images by the CNN.Moreover,the CNN has a certain illuminance generalization ability capable of detection temperature information from the images which were collected under different illuminance and were not used for training.Compared to the conventional machine learning algorithms mentioned in the recent literatures,this real-time,contact-free temperature measurement approach that does not require any further image processing operations facilitates temperature monitoring applications in the industrial and civil fields.

Therapeutic effect of secretome from TNF-αstimulated mesenchymal stem cells in an experimental model of corneal limbal stem cell deficiency

AIM:To explore the secretome efficacy in tumor necrosis factor(TNF)-αstimulated mouse mesenchymal stem cells(MSCs)in a murine model of corneal limbal alkali injury.METHODS:Corneal limbal stem cell deficiency(LSCD)was created in the eyes of male C57 mice.Concentrated conditioned medium from TNF-αstimulated MSCs(MSC-CMT)was applied topically for 4 wk,with basal medium and conditioned medium from MSCs as controls.Corneal opacification,corneal inflammatory response,and corneal neovascularization(NV)were evaluated.Corneal epithelial cell apoptosis,corneal conjunctivation,and inflammatory cell infiltration were assessed with TUNEL staining,CK3 and Muc-5 AC immunostaining,and CD11 b immunofluorescence staining,respectively.The effect of TSG-6 was further evaluated by knockdown with short hairpin RNA(sh RNA).RESULTS:Compared to the controls,topical administration of MSC-CMT significantly ameliorated the clinical symptoms of alkali-induced LSCD,with restrained corneal NV,reduced corneal epithelial cell apoptosis,and inhibition of corneal conjunctivation.In addition,MSC-CMT treatment significantly reduced CD11 b+inflammatory cell infiltration,and inhibited the expression of pro-inflammatory cytokines(IL-1β,TNF-αand IL-6).Furthermore,the promotion of corneal epithelial reconstruction by MSC-CMT was largely abolished by TSG-6 knockdown.CONCLUSION:Our study provides evidence that MSCCMT enhances the alleviation of corneal alkali injuries,partially through TSG-6-mediated anti-inflammatory protective mechanisms.MSC-CMT may serve as a potential strategy for treating corneal disorders.

Exosomes exist in nipple discharge of breast cancer

Exosomes are a class of membranous vesicles,which are approximately 50 to 100 nm in diameter;they are released into the extracellular matrix from various cells.[1]Exosomes are rich in proteins,lipids,and nucleic acids.They have been reported to be involved in various physiological processes and are important tools for intercellular material and information communication.[2,3]Increasing evidence has indicated that exosomes can be extracted from human saliva,urine,blood,and other body fluids.[4]However,whether exosomes exist in nipple discharge remains unknown.In this study,exosomes from nipple discharge of breast cancer patients and colostrum of postpartum women were isolated using ultracentrifugation.The exosomes were identified by transmission electron microscopy(TEM),Western blot,and nanoparticle tracking analysis(NTA).The detailed methods are shown in Supplementary File 1,http://links.lww.com/CM9/A286.The study was approved by the Ethics Committee of Qilu Hospital of Shandong University(No.KYLL-2018-096),and all patients provided written informed consent.