Fe_(3)O_(4) nanoparticles(NPs)are widely used in the construction of drug and gene delivery vectors because of their particular physicochemical properties.Surface modification can not only reduce the cytotoxicity of F...Fe_(3)O_(4) nanoparticles(NPs)are widely used in the construction of drug and gene delivery vectors because of their particular physicochemical properties.Surface modification can not only reduce the cytotoxicity of Fe_(3)O_(4),but also further improve the biocompatibility and delivery efficiency.In this work,firstly,polydopamine(PDA)-coated Fe_(3)O_(4) NPs(named Fe_(3)O_(4)@PDA)were prepared by using the self-polymerization characteristics of dopamine in alkaline environment.Then,polyamidoamine(PAMAM)was modified by the Michael addition reaction to prepare water-soluble core shell magnetic NPs of Fe_(3)O_(4)@PDA@PAMAM,and its potential as gene vector was further evaluated.The results revealed that Fe_(3)O_(4)@PDA@PAMAM had the ability to condense and protect DNA,and showed lower cytotoxicity,higher cell uptake and transfection efficiency than those of PAMAM.It has the potential to become a magnetic targeted gene vector for further study.展开更多
基金This work was supported in part by the National Natural Science Foundation of China(Grant No.21602166)the Natural Science Foundation of Hubei Province(Grant No.2020CFB760)the Research and Innovation Initiatives of WHPU(Grant No.2021Y11).
文摘Fe_(3)O_(4) nanoparticles(NPs)are widely used in the construction of drug and gene delivery vectors because of their particular physicochemical properties.Surface modification can not only reduce the cytotoxicity of Fe_(3)O_(4),but also further improve the biocompatibility and delivery efficiency.In this work,firstly,polydopamine(PDA)-coated Fe_(3)O_(4) NPs(named Fe_(3)O_(4)@PDA)were prepared by using the self-polymerization characteristics of dopamine in alkaline environment.Then,polyamidoamine(PAMAM)was modified by the Michael addition reaction to prepare water-soluble core shell magnetic NPs of Fe_(3)O_(4)@PDA@PAMAM,and its potential as gene vector was further evaluated.The results revealed that Fe_(3)O_(4)@PDA@PAMAM had the ability to condense and protect DNA,and showed lower cytotoxicity,higher cell uptake and transfection efficiency than those of PAMAM.It has the potential to become a magnetic targeted gene vector for further study.
