两起发热伴血小板减少综合征聚集性疫情流行病学及病原学分析

发热伴血小板减少综合征(severe fever with thrombocytopenia syndrome,SFTS)是一种新发传染病,主要通过蜱虫叮咬传播,其病原体为发热伴血小板减少综合征病毒(severe fever with thrombocytopenia syndrome virus,SFTSV)。人际传播引发的SFTS聚集性疫情在国内外均有报道,人们重点关注了人际传播的感染途径,然而SFTS聚集性疫情和病毒基因型之间的相关性研究却未见报道。本文主要报道了2022~2023年河南省信阳市发生的两起SFTS聚集性疫情,探讨了SFTSV出现人际传播感染的可能途径,并对SFTS聚集性疫情与病毒基因型进行了关联分析。通过4例确诊患者的病毒序列分析,发现两起聚集性疫情中的2组SFTSV分别聚集在隶属于不同基因型的两个分支。将本研究病毒序列与GenBank中获得的SFTS聚集性疫情报道过的病毒序列进行系统发育分析,进一步发现人际传播病例报道的SFTSV涉及3种基因型,提示SFTS聚集性疫情的发生可能和病毒基因型无明显关联。本研究表明血液接触感染可能是SFTS聚集性疫情发生的主要传播途径,为揭示SFTS聚集性疫情的人际传播链提供了遗传学证据,为SFTS的人际传播防控提供了科学数据支撑。

Mechanism of gas pressure action during the initial failure of coal containing gas and its application for an outburst inoculation

Faced with the continuous occurrence of coal and gas outburst(hereinafter referred to as“outburst”)disasters,as a main controlling factor in the evolution process of an outburst,for gas pressure,it is still unclear about the phased characteristics of the coupling process with in situ stress,which induce coal damage and instability.Therefore,in the work based on the mining stress paths induced by typical outburst accidents,the gradual and sudden change of three-dimensional stress is taken as the background for the mechanical reconstruction of the disaster process.Then the true triaxial physical experiments are conducted on the damage and instability of coal containing gas under multiple stress paths.Finally,the response characterization between coal damage and gas pressure has been clarified,revealing the mechanism of action of gas pressure during the initial failure of coals.And the main controlling mechanism during the outburst process is elucidated in the coupling process of in situ stress with gas pressure.The results show that during the process of stress loading and unloading,the original gas pressure enters the processes of strengthening and weakening the action ability successively.And the strengthening effect continues to the period of large-scale destruction of coals.The mechanical process of gas pressure during the initial failure of coals can be divided into three stages:the enhancement of strengthening action ability,the decrease of strengthening action ability,and the weakening action ability.The entire process is implemented by changing the dominant action of in situ stress into the dominant action of gas pressure.The failure strength of coals is not only affected by its original mechanical strength,but also by the stress loading and unloading paths,showing a particularly significant effect.Three stages can be divided during outburst inoculation process.That is,firstly,the coals suffer from initial damage through the dominant action of in situ stress with synergy of gas pressure;secondly,the coals with spallation of structural division are generated through the dominant action of gas pressure with synergy of in situ stress,accompanied by further fragmentation;and finally,the fractured coals suffer from fragmentation and pulverization with the gas pressure action.Accordingly,the final broken coals are ejected out with the gas action,initiating an outburst.The research results can provide a new perspective for deepening the understanding of coal and gas outburst mechanism,laying a theoretical foundation for the innovation of outburst prevention and control technologies.

Accumulation of carotenoids and expression of carotenoid biosynthesis genes in fruit flesh during fruit development in two Cucurbita maxima inbred lines

Mesocarp color is an important agronomic trait of Cucurbita maxima and is determined mainly by the contents and compositions of the carotenoids.The two inbred lines with significant differences in fruit flesh color were used in the study,the orange’312-1’and white’98-2’.Changes in seven carotenoid contents and compositions in the flesh of fruit produced by inbred lines’312-1’and’98-2’were analyzed during fruit development.The expression of eight key carotenoid biosynthesis genes in the fruit flesh were investigated during five fruit development stage in two inbred lines.As the flesh color intensified,the orange flesh color of’312-1’was determined mainly by the increased contents of lutein,β-carotene and zeaxanthin and the lack of carotenoid accumulation led to the formation of white flesh in’98-2’fruit.The expression of the LCY-e and CHYb genes was significantly stronger in’312-1’than in’98-2’,and their expression was strongly correlated with lutein andβ-carotene contents during fruit development.This study provides a deep understanding of the molecular mechanism of carotenoid biosynthesis in fruit flesh and provides a basis for additional studies on the highly refined improvement of squash quality.

Enhanced stability of Pt/Al_(2)O_(3) modified by Zn promoter for catalytic dehydrogenation of ethane

Catalytic ethane dehydrogenation(EDH) to ethylene over Pt-based catalysts has received increasing interests in recent years as it is a potential alternative route to conventional steam cracking. However, the catalysts used in this reaction often suffer from rapid deactivation due to serious coke deposition and metal sintering. Herein, we reported the effects of Zn modification on the stability of Pt/Al2 O3 for EDH.The Zn-modified sample(PtZn2/Al2 O3) exhibits stable ethane conversion(20%) with over 95% ethylene selectivity. More importantly, it exhibits a significantly low deactivation rate of only 0.003 h-1 at 600 °C for70 h, which surpasses most of previously reported catalysts. Detailed characterizations including in situ FT-IR, ethylene adsorption microcalorimetry, and HAADF-STEM etc. reveal that Zn modifier reduces the number of Lewis acid sites on the catalyst surface. Moreover, it could modify Pt sites and preferentially cover the step sites, which decrease surface energy and retard the sintering of Pt particle, then prohibiting the further dehydrogenation of ethylene to ethylidyne. Consequently, the good stability is realized due to anti-sintering and the decrease of coke formation on the Pt Zn2/Al2 O3 catalyst.

A novel carbon cycle process assisted by Ni/La_(2)O_(3) catalyst for enhanced thermochemical CO_(2) splitting

Thermochemical two-step CO_(2) splitting is a potential approach that fixes the sustainable resource into transportable liquid fuels.However,the harsh CO_(2) splitting conditions,the limited oxygen release kinetics and capacity of metal oxides block further promoted the CO yield and solar-to-fuel energy efficiency.Here,we propose a different carbon cycle assisted by Ni/La_(2)O_(3) via coupling methane decomposition with thermochemical CO_(2) splitting,replacing conventional metal oxides cycle.Superior performance was demonstrated with methane conversion reached around 94%with almost pure H_(2) generation.Encouragingly,CO_(2) conversion of 98%and CO yield of 6.9 mmol g^(-1) derived from CO_(2) were achieved,with peak CO evolution rate(402 mL min^(-1) g^(-1))of orders of magnitude higher than that in metal oxide process and outstanding thermodynamic solar-to-fuel energy efficiency(55.5%vs.18.5%).This was relevant to the synergistic activation of La_(2)O_(3) and Ni for CO_(2) in carbon cycle,thus improving CO_(2) splitting reaction with carbon species.

Morphology and Function of the Aortic Valve after Transcatheter Closure of Ventricular Septal Defect with Aortic Valve Prolapse

Objective:This study aims to evaluate the morphology and function of the aortic valve after transcatheter closure of ventricular septal defect(VSD)with aortic valve prolapse(AVP)abased on clinical and radiological outcomes.Methods:From January 2013 to November 2014,164 consecutive patients(97 males,59.1%)with VSD and AVP were treated by transcatheter closure.The patients were divided into the mild AVP group(n=63),moderate AVP group(n=89)and severe AVP group(n=12).The clinical and radiological outcomes of these patients were analyzed retrospectively.Results:In total,146(89.0%)patients were successfully treated with VSD occluders,including 59/63(93.7%)with mild AVP,80/89(89.9%)with moderate AVP and 7/12(58.3%)with severe AVP.The degree of AVP was ameliorated or disappeared in 39(26.7%)patients,and remained unchanged in 103(70.5%)patients after the intervention.In the 35 patients who initially had trivial-to-moderate aortic regurgitation(AR),the degree of AR was ameliorated or disappeared in 25(71.4%)patients,aggravated from trivial to mild AR in 1(2.9%)patient,and remained unchanged in 9(25.7%)patients.In 111 patients without AR,1(0.9%)patient had mild AR and 24(21.6%)patients had trivial AR after intervention.The depth and width of the prolapsed aortic valve decreased after transcatheter closure of VSD in all three groups.During the 70-month(range,54–77)follow-up period,no patients with AVP and AR needed an aortic valve intervention.Conclusions:Transcatheter closure of VSD with AVP is feasible.The morphology and function of the prolapsed aortic valve improved and remained stable for a long period after intervention.

MINS, a Novel Naphthalimide-Polyamine Conjugate, Induced Apoptosis Depending on p53 Status in Human Colon Cancer Cells

Natural polyamine is an ideal antitumor drug carrier because of the great requirement difference between cancer cells and normal cells. Previous data demonstrated that many cytotoxic drugs conjugated with natural or synthetic polyamines have potent antitumor effects. Up to now, the antitumor mechanism of conjugates of naphthalimides with polyamine remains poorly understood in human colon cancer cells. The aim of this study is to evaluate the effect of MINS (a novel naph-thalimide-polyamine conjugate) and mechanism of MINS in human colon cancer cells. Mitochondrial toxicity, which was generated by ROS from mitochondria electron transport chain, might be a major factor in MINS-inducted apoptosis. Our data also demonstrated that MINS-mediated cell apoptosis depend on p53 status, for MINS induced Caco-2 cells (p53 null) only necrosis but not apoptosis. Furthermore, the apoptotic effect of MINS is stronger than that of Amonafide, the parent drug of MINS. Our data suggested that the MINS-mediated cell apoptosis depend on p53 in human colon cancer cells.

PGAM5-Mediated PHB2 Dephosphorylation Contributes to Diabetic Cardiomyopathy by Disrupting Mitochondrial Quality Surveillance

Disruption of the mitochondrial quality surveillance(MQS)system contributes to mitochondrial dysfunction in diabetic cardiomyopathy(DCM).In this study,we observed that cardiac expression of phosphoglycerate mutase 5(PGAM5),a mitochondrial Ser/Thr protein phosphatase,is upregulated in mice with streptozotocin-induced DCM.Notably,DCM-related cardiac structural and functional deficits were negated in cardiomyocyte-specific Pgam5 knockout(Pgam5^(CKO))mice.Hyperglycemic stress impaired adenosine triphosphate production,reduced respiratory activity,and prolonged mitochondrial permeability transition pore opening in acutely isolated neonatal cardiomyocytes from control Pgam5^(f/f) mice,and these effects were markedly prevented in cardiomyocytes from Pgam5^(CKO) mice.Likewise,three main MQS-governed processes—namely,mitochondrial fission/fusion cycling,mitophagy,and biogenesis—were disrupted by hyperglycemia in Pgam5^(f/f),but not in Pgam5^(CKO),cardiomyocytes.On the basis of bioinformatics prediction of interaction between PGAM5 and prohibitin 2(PHB2),an inner mitochondrial membrane-associated scaffolding protein,co-immunoprecipitation,and immunoblot assays demonstrated that PGAM5 dephosphorylates PHB2 on Ser91.Transfection of cardiomyocytes with phosphodefective or phosphomimetic Ser91 mutants of PHB2 confirmed a critical role for PGAM5-mediated dephosphorylation of PHB2 in mitochondrial dysfunction associated with hyperglycemic stress.Furthermore,knockin mice expressing phosphomimetic PHB2^(S91D) were resistant to diabetes-induced cardiac dysfunction.Our findings highlight the PGAM-PHB2 axis as a novel and critical regulator of mitochondrial dysfunction in DCM.

ETME,a novel β-elemene derivative,synergizes with arsenic trioxide in inducing apoptosis and cell cycle arrest in hepatocarcinoma cells via a p53-dependent pathway

Arsenic trioxide(ATO)has been identified as an effective treatment for acute promyelocytic leukemia(APL)but is much less effective against solid tumors such as hepatocellular carcinoma(HCC).In the search for ways to enhance its therapeutic efficacy against solid tumors,we have examined its use in combination with a novel derivative ofβ-elemene,N-(β-elemene-13-yl)tryptophan methyl ester(ETME).Here we report the effects of the combination on cell viability,apoptosis,the cell cycle and mitochondria membrane potential(MMP)in HCC SMMC-7721 cells.We found that the two compounds acted synergistically to enhance antiproliferative activity and apoptosis.The combination also decreased the MMP,down-regulated Bcl-2 and pro-proteins of the caspase family,and up-regulated Bax and BID,all of which were reversed by the p53 inhibitor,pifithrin-α.In addition,the combination induced cell cycle arrest at the G2/M phase and reduced tumor volume and weight in an xenograft model of nude mice.Overall,the results suggest that ETME in combination with ATO may be useful in the treatment of HCC patients particularly those unresponsive to ATO alone.

Adult-repopulating lymphoid potential of yolk sac blood vessels is not confined to arterial endothelial cells

During embryogenesis,hematopoietic stem progenitor cells(HSPCs)are believed to be derived from hemogenic endothelial cells(HECs).Moreover,arterial feature is proposed to be a prerequisite for HECs to generate HSPCs with lymphoid potential.Although the molecular basis of hematopoietic stem cell-competent HECs has been delicately elucidated within the embryo proper,the functional and molecular characteristics of HECs in the extraembryonic yolk sac(YS)remain largely unresolved.In this study,we initially identified six molecularly different endothelial populations in the midgestational YS through integrated analysis of several single-cell RNA sequencing(scRNA-seq)datasets and validated the arterial vasculature distribution of Gja5+ECs using a Gja5-EGFP reporter mouse model.Further,we explored the hemogenic potential of different EC populations based on their Gja5-EGFP and CD44 expression levels.The hemogenic potential was ubiquitously detected in spatiotemporally different vascular beds on embryonic days(E)8.5–E9.5 and gradually concentrated in CD44-positive ECs from E10.0.Unexpectedly,B-lymphoid potential was detected in the YS ECs as early as E8.5 regardless of their arterial features.Furthermore,the capacity for generating hematopoietic progenitors with in vivo lymphoid potential was found in nonarterial as well as arterial YS ECs on E10.0–E10.5.Importantly,the distinct identities of E10.0–E10.5 HECs between YS and intraembryonic caudal region were revealed by further scRNA-seq analysis.Cumulatively,these findings extend our knowledge regarding the hemogenic potential of ECs from anatomically and molecularly different vascular beds,providing a theoretical basis for better understanding the sources of HSPCs during mammalian development.

Design, synthesis and evaluation of genistein-polyamine conjugates as multi-functional anti-Alzheimer agents

A series of genistein-polyamine conjugates(4a–4h) were designed, synthesized and evaluated as multi-functional anti-Alzheimer agents. The results showed that these compounds had significant cholinesterases(Ch Es) inhibitory activity. Compound 4b exhibited the strongest inhibition to acetylcholinesterase(ACh E) with an IC_(50) value of 2.75 μmol/L, which was better than that of rivastigmine(5.60 μmol/L). Lineweaver–Burk plot and molecular modeling study showed that compound 4b targeted both the catalytic active site(CAS) and the peripheral anionic site(PAS) of ACh E. Besides, compound 4b showed potent metal-chelating ability. In addition, it was found that 4a–4h did not affect Hep G-2 cell viability at the concentration of 10 μmol/L.

Synthesis, Cytotoxic Activity Evaluation of Novel 1,2,3-Triazole Linked Quinazoline Derivatives

A series of novel 1,2,3-triazole-quinazoline derivatives were synthesized in five steps starting from anthranila- mide by conventional methods. All the title compounds 10a-10r were evaluated for cytotoxic activity against four human cancer cell lines （MGC-803, EC-109, MCF-7 and HGC-27） using MTT assay in vitro. Some of the synthe- sized compounds exhibited moderate to potent activity against tested cancer cell lines. Among them, compounds 10h and 10q exhibited excellent growth inhibition against HGC-27 and compound 10m also possessed excellent ac- tivity against MCF-7, with IC50 values less than 1 μmol/L. Especially, compound 10h was more cytotoxic than 5-fluorouracil against all tested four human cancer cell lines.

Incorporation of Ag into Cu(In,Ga)Se_(2) films in low-temperature process

Chalcopyrite Cu(In,Ga)Se_(2)(CIGS) thin films deposited in a low-temperature process(450℃) usually produce fine grains and poor crystallinity. Herein, different Ag treatment processes, which can decrease the melting temperature and enlarge band gap of the CIGS films, were employed to enhance the quality of thin films in a low-temperature deposition process. It is demonstrated that both the Ag precursor and Ag surface treatment process can heighten the crystallinity of CIGS films and the device efficiency. The former is more obvious than the latter. Furthermore, the Urbach energy is also reduced with Ag doping. This work aims to provide a feasible Ag-doping process for the high-quality CIGS films in a low-temperature process.